• Journal of Forest and Environmental Science
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• 제25권2호
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• pp.93-100
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• 2009

In Malaysia, Labisia pumila Benth & Hook f, popularly known as 'Kacip Fatimah' has been used traditionally to treat various elements of the woman's health in Malay community. The objective of this study was to develop randomly amplified polymorphic DNA (RAPD) based DNA markers for the identification of L. pumila and to distinguish its three varieties from each other. Total DNA from nine accessions of L. pumila was extracted by CTAB method and polymerase chain reactions (PCR) were carried out to amplify the segments of DNA using different primers to develop DNA barcode using RAPD technique. To find out variety-specific DNA marker/s, twenty different 10-mer primer sequences with annealing temperature from 36-$40^{\circ}C$ were evaluated in triplicate. Out of 20 random primers, two primers (OPA-1 and OPA-2/A10) were selected which produced reliable RAPD band patterns. To have DNA based handle, two RAPD amplification products were cloned and sequenced to determine the identity of the DNA. RAPD analysis using two random primers generated 72 discrete bands ranging in size 200 bp-3,000 bp. Fifty nine of these were polymorphic loci (82%) and thirteen were non-polymorphic loci (18%). A total of 32 bands polymorphic loci (72%) were amplified with primer OPA-1 and analyzed by cluster analysis and UPGMA (Unweighted Pair Group Method with Arithmetic) to present a dendogram depicting the degree of genetic relationship among nine accessions of L. pumila. Our results shows the reasonable genetic diversity among the L. pumila varieties and within varieties; and two RAPD marker sequences obtained could be used to identify L. pumila at species level.

• Journal of Animal Science and Technology
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• 제54권6호
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• pp.401-409
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• 2012

The objective of this study was to determine genetic variation of five Korean native chicken lines using 30 microsatellite (MS) markers, which were previously recommended by ISAG (International Society for Animal Genetics). The initial study indicated that two microsatellite markers, MCW0284 and LEI0192, were not amplified in these lines and excluded for further analysis. Twenty eight microsatellite markers were investigated in 83 birds from five Korean native chicken lines. The identified mean number of alleles was 4.57. Also, the expected, observed heterozygosity (He, Ho) and polymorphism information content (PIC) values were estimated in these markers and they ranged from 0.31~0.868, 0.145~0.699, and 0.268~0.847, respectively. The results were used for the discrimination of five chicken lines using genetic distance values and also neighbor-joining phylogenetic tree was constructed. Based on the He and PIC values, eighteen markers are enough for the discrimination of these Korean native chicken lines for the expected probability of identity values among genotypes of random individuals (PI), random half sibs ($PI_{half-sibs}$) and random sibs ($PI_{sibs}$). Taken together, these results will help the decision of conservation strategies and establishment of traceability system in this native chicken breed. Also, the use of ISAG-recommended microsatellite markers may indicate that the global comparison with other chicken breeds is possible.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.25-25
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• 2018

The Fukushima Daiichi Nuclear Power Plant accident in March 2011 caused severe radioactive contamination in the surrounding environment. Since the accident, much attention has been paid to the biological and genetic consequences of organism inhabiting the contaminated area. The effect of radiation exposure on genetic mutation rates is little known, especially for low doses and in situ conditions. Evaluating DNA mutation by low levels of radiation dose is difficult due to the rare mutation event and lack of sequence information before the accident. In this study, correlations with air dose levels and somatic DNA mutation rates were evaluated using Next Generation Sequencer for the clonal plant, Phyllostachys edulis. This bamboo is known to spread an identical clone throughout Japan, and it has the advantage that we can compare genetic mutation rate among identical clone growing different air dose levels. We collected 94 samples of P. edulis from 14 sites with air dose rates from $0.04{\sim}7.80{\mu}Gy/h$. Their clonal identity was confirmed by analysis using 24 microsatellite markers, and then, sequences among samples were compared by MIG sequence. The sequence data were obtained from 2,718 loci. About ~200,000 bp sequence (80 bp X 2,718 loci) were obtained for each sample, and this corresponds to about 0.01% of the genome sequence of P. edulis. In these sequences, 442 loci showed polymorphism patterns including recent origin mutation, old mutation, and sequence errors. The number of mutations per sample ranged from 0 to 13, and did not correlate with air dose levels. This result indicated that DNA mutations have not accumulated in P. edulis living in the air doses levels less than $10{\mu}Gy/h$. Our study also suggests that mutation rates can be assessed by selecting an appropriate experimental approach and analyzing with next generation sequencer.

• 한국동물위생학회지
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• 제32권1호
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• pp.1-10
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• 2009

In order to obtain the genetic information of the Korean isolates of porcine circovirus 2 (PCV2), complete genomes of five isolates from Korean PCVD weaned pigs with wasting syndromes were sequenced and compared with those of other published PCV2 isolates. Of the five PCV2 isolates, four (1767 nucleotides) were classified into PCV2b, and one (1,768 nucleotides) was PCV2a. Moreover, it appeared that PCV2b is now the dominant genotype circulating in Korea herds. Total complete genomes of four PCV2b isolates shared $99.1{\sim}99.4%$ nucleotide sequence homology each other, and were only $95.4{\sim}96.2%$ similar to one PCV2a isolate. ORF2 genome of four PCV2b isolates shared over 99% nucleotide sequence and deduced amino acid sequence identity to each other. Nevertheless, those were much divergent with the PCV2a isolate of this study and ranged from $92.3{\sim}92.7%$ nucleotide homology and $91.9{\sim}92.3%$ deduced amino acid sequence homology, respectively. The amino acid sequence alignments of the putative capsid protein identified three major regions of amino acid heterogeneity at residues $59{\sim}91$, $121{\sim}136$ and $190{\sim}210$. Two of those correspond with dominant immunoreactive areas. Phylogenetic analysis based on the complete genome of PCV2 isolates showed that four PCV2b isolates of this study existed the closest relationship with European strains (Netherland, UK and France). One PCV2a isolate was closely related to Japan and North America strains.

• Journal of Animal Science and Technology
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• 제55권3호
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• pp.185-194
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• 2013

MS 마커는 가축의 유전적 다양성, 유연관계 및 품종식별의 연구에 있어서 매우 유용하다. 본 연구는 26개의 MS 마커를 이용하여 토종닭 브랜드 2집단(우리맛닭, 한협3호)과 토착종 순계 2집단(화이트 레그혼, 로드 아일랜드 레드)을 대상으로 집단내 및 집단간의 유전적 다양성, 계통유전학적 관계, 유전적 균일성 등을 검증하여 고유 유전자원으로서의 가치 구명 및 개체식별력이 높은 마커를 선별하여 토종닭 브랜드 계육의 생산이력시스템에 활용 가능한 기초자료를 제시하고자 실시 하였다. 대립 유전자형 분석결과 총 191개 중 47개(24.6%)가 집단 특이 대립 유전자였으며, 다형성지수의 평균은 $H_{Exp}$=0.667, PIC=0.630으로 산출 되었다. 공시된 320수 개체에 대한 요인대응분석(FCA) 결과 4개의 군집을 형성하였지만 2개 토종닭 브랜드 집단은 타 집단에 비해 매우 가까운 거리에 위치하고 있었으며, 집단간의 $D_A$ 유전거리 결과 또한 이와 동일했다. 각 집단에 대한 유전적 균일도는 모든 집단에서 94% 이상으로 높았다. 이상의 결과는 2개 토종닭 브랜드 집단은 유전적으로 유사하지만 토종닭 순계 집단과는 유전적인 차이가 크며, 순계 2집단 또한 유전적으로 확연히 분리됨을 증명 할 수 있다. 26개 MS마커 사용시 동일개체 출현확률(PI)은 $1.17{\times}10^{-49}$였다. 2011년 기준으로 닭 사육수수 149,511,309수를 고려해 PI 값이 높은 마커 9~12개 정도를 선별 및 분석에 이용 한다면 동일개체 출현확률(PI)이 $1.14{\times}10^{-15}$에서 $7.33{\times}10^{-20}$이므로 개체식별 및 친자 감별이 가능할 것으로 사료된다. 향후 경제적 효율성을 고려하여 MS 마커 및 mtDNA의 SNP를 기반으로 multiplexing PCR 시스템을 확립을 위한 연구가 이행된다면 토종닭 브랜드육의 생산이력시스템에 적용이 가능할 것으로 판단된다.

• 식물병연구
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• 제9권4호
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• pp.189-195
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• 2003

참외의 발효과를 유발하는 세균은 16S rDNA 염기분석에 의해 3 group으로 분류되었다. CM2105균주의 16S rDNA 염기서열은 Microbacterium phyllosphaerae의 염기서열과 99.6%의 높은 상동성을 나타냈고, M. holiorum과도 99.5%의 높은 상동성을 나타냈다. CM2101과 CM2121균주의 16S rDNA 염기서열은 "P. pavonaceae"와 각각 98.9%, 98.8, CM2126균주는 P. costantinii와 99.5%, P. grimontii와 99.0%의 높은 상동성을 나타냈다. CM21131균주의 16S rDNA 염기서열은 Enterobacter cloacae와 99.7%의 높은 상동성 나타냈다. 검정균주의 생리적, 생화학적 특성과 16S rDNA의 염기분석에 따라 CM2105 균주는 M. phyllosphaerae, CM2101, CM2121, CM2126 균주는 Pseudomonas spp., 그리고 CM2113 균주는 E. cloacae로 동정하였다.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2003년도 ICCAS
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• pp.835-838
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• 2003

Computer vision and natural-language dialogue play an important role in friendly human-machine interfaces for service robots. In this paper we describe an integrated face detection and face recognition system for a welfare robot, which has also been combined with the robot's speech interface. Our approach to face detection is to combine neural network (NN) and genetic algorithm (GA): ANN serves as a face filter while GA is used to search the image efficiently. When the face is detected, embedded Hidden Markov Model (EMM) is used to determine its identity. A real-time system has been created by combining the face detection and recognition techniques. When motivated by the speaker's voice commands, it takes an image from the camera, finds the face inside the image and recognizes it. Experiments on an indoor environment with complex backgrounds showed that a recognition rate of more than 88% can be achieved.

• 한국경영과학회:학술대회논문집
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• 한국경영과학회/대한산업공학회 2005년도 춘계공동학술대회 발표논문
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• pp.614-620
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• 2005

Ensemble에서 feature selection은 각 classifier의 학습할 데이터의 변수를 다르게 하여 diversity를 높이며, 이것은 일반적인 성능향상을 가져온다. Feature selection을 할 때 쓰는 방법 중의 하나가 Genetic Algorithm (GA)이며, GA-SVM은 GA를 기본으로 한 wrapper based feature selection mechanism으로 response model과 keystroke dynamics identity verification model을 만들 때 좋은 성능을 보였다. 하지만 population 안의 후보들간의 diversity를 보장해주지 못한다는 단점 때문에 classifier들의 accuracy와 diversity의 균형을 맞추기 위한 heuristic parameter setting이 존재하며 이를 조정해야만 하였다. 우리는 GA-SVM 알고리즘을 바탕으로, population안 후보들의 fitness를 측정할 때 accuracy와 diversity 둘 다 고려하는 fitness function을 도입하여 추가적인 classifier 선택 작업을 제거하면서 성능을 유지시키는 방안을 연구하였으며 결과적으로 알고리즘의 복잡성을 줄이면서도 모델의 성능을 유지시켰다.

• BMB Reports
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• 제32권5호
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• pp.502-505
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• 1999

cDNA fragments of ovine liver pyridoxal kinase were amplified by PCR using degenerate oligonucleotide primers derived from partial amino acids sequences of the enzyme. Using PCR products as probes, several overlapping cDNA clones were isolated independently from an ovine liver and a human brain cDNA library. The largest cDNA clone for each was selected for sequence analysis. The ovine liver cDNA encodes a polypeptide of 297 amino acid residues with Mr of 32,925, whereas the human clone is comprised of an open reading frame encoding 312 amino acid residues with Mr of 35,102. The deduced sequence of the human brain enzyme is completely identical to that of human testes cDNA recently reported (Hanna et al., 1997). The ovine enzymes have approximately 77% sequence identity with the human enzyme although the two sequences are completely different in the N-terminus comprising 32 residues. This result suggests that pyridoxal kinase is highly homologous in mammalian species.

• 대한가정학회지
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• 제36권2호
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• pp.145-160
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• 1998

This study was designed to investigate purchasing and post-purchase behaviors of middle-aged wives in buying townwears, an well as to examine socio-demographic variables, physical variables, psychological variables, and their influences on. The data included 374 housewives in Seoul and Pusan. Statistics used for data analysis were Frequencies, Means, Standard Deviation, t-test, ANOVA, Pearson's Correlation. The major finding are as follows: 1. The level of post-purchase satisfaction marked higher than middle point. 2. Post-purchase satisfaction differed significantly in income, health-state, identity, stress perceptivity, income satisfaction, sense of comparison, and propensity to consume.