• Korean Journal of Environmental Biology
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• v.36 no.2
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• pp.165-173
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• 2018

We sampled vomit of black-faced spoonbills(Platalea minor) during the brood-rearing season (from June 2011 to June 2014) at the Namdong reservoir in Incheon and analyzed the food components in the vomit using microscopy and next-generation sequencing (NGS). Microscopic observations primarily helped in identifying osteichthyes (bony fishes), crustaceans, and polychaetes. In particular, species belonging to the families Mugilidae and Gobiidae among the fish, and Macrophthalmus japonicas among the crustaceans, were observed at high frequency. Results of NGS analysis revealed the predominant presence of bony fish (42.58% of total reads) and crustaceans (40.75% of total reads), whereas others, such as polychaetes (12.66%), insects (0.24%), and unidentified species (3.78%), occurred in lower proportions. At the species level, results of NGS analysis revealed that Macrophthalmus abbreviates and Macrobrachium sp. among the crustaceans, and Acanthogobius hasta, Tridentiger obscurus, and Pterogobius zacalles among the bony fish, made up a high proportion of the total reads. These food species are frequently found at tidal flats in the Songdo and Sihwa lakes, emphasizing the importance of these areas as potential feeding sites of the black-faced spoonbill. Feed composition of the black-faced spoonbill, as evaluated by analyzing its vomit, differed when the evaluations were done by microscopic observation or by NGS analysis. Evaluation by microscopic observation is difficult and not error free, owing to the degradation of the samples to be analyzed; however, NGS analysis is more accurate, because it makes use of genetic information. Therefore, accurately analyzing food components from morphologically indistinguishable samples is possible by using genetic analysis.

• Journal of Animal Science and Technology
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• v.53 no.4
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• pp.297-302
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• 2011

This study was carried out to estimate average inbreeding coefficients, relatedness and effective population size of breeding bulls and to suggest optimal alternatives on problems of current Hanwoo improvement system. Data on proven and young bulls were obtained from 1,128 heads of Livestock Improvement Main Center from 1983 to 2008. Pedigree information on proven and young bulls was obtained from 3,760 heads of Korea Animal Improvement Association. Average inbreeding coefficients and average relatedness of proven and young bulls were estimated at the range of 0.04-0.07%, 0.10-6.82%, respectively. Effective population size was estimated for 220 heads from the average rate of inbreeding of last 26 years. Average inbreeding coefficient is rising rapidly for the last two years as well as average relatedness. Effective population size was estimated for 47 heads for the last five years. These results suggest that selection criteria of proven bulls should include not only genetic evaluation of carcass performance from progeny-test, but also inbreeding and relationship coefficients, in order to maintain genetic variability of Hanwoo. In addition, effective population size should be increased by increasing the number of proven bulls.

• Korean Journal of Poultry Science
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• v.40 no.3
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• pp.223-234
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• 2013

Poultry industry is abounding day by day as it engrosses less cost of investment per bird as compared to large animals. Poultry have the most copious genomic tool box amongst domestic animals for the detection of quantitative trait loci (QTL) and marker assisted selection (MAS). Use of multiple markers and least square techniques for mapping of QTL affecting quality and production traits in poultry is in vogue. Examples of genetic tests that are available to or used in industry programs are documented and classified into causative mutations (direct markers), linked markers in population-wide linkage disequilibrium (LD) with the QTL (LD markers), and linked markers in population wide equilibrium with the QTL (LE markers). Development of genome-wide SNP assays, role of 42 K, 60 K (Illumina) and 600 K (Affymetrix$^{(R)}$ Axim$^{(R)}$) SNP chip with next generation sequencing for identification of single nucleotide polymorphism (SNP) has been documented. Hybridization based, PCR based, DNA chip and sequencing based are the major segments of DNA markers which help in conducting of MAS in poultry. Economic index-marker assisted selection (EI-MAS) provides platform for simultaneous selection for production traits while giving due weightage to their marginal economic values by calculating predicted breeding value, using information on DNA markers which are normally associated with relevant QTL. Understanding of linkage equilibrium, linkage dis-equilibrium, relation between the markers and gene of interest are quite important for success of MAS. This kind of selection is the most useful tool in enhancing disease resistance by identifying candidate genes to improve the immune response. The application of marker assisted selection in selection procedures would help in improvement of economic traits in poultry.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.26 no.1
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• pp.69-77
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• 2013

When structural design of a tall building is conducted, reduction of wind-induced lateral displacement is one of the most important problem. For this purpose, additional dampers and vibration control devices are generally considered. In this process, control performance of additional devices are usually investigated for optimal design without variation of characteristics of a structure. In this study, multi-objective integrated optimization of structure-smart control device is conducted and possibility of reduction of structural resources of a tall building with additional smart damping device has been investigated. To this end, a 60-story diagrid building structure is used as an example structure and artificial wind loads are used for evaluation of wind-induced responses. An MR damper is added to the conventional TMD to develop a smart TMD. Because dynamic responses and the amount of structural material and additional smart damping devices are required to be reduced, a multi-objective genetic algorithm is employed in this study. After numerical simulation, various optimal designs that can satisfy control performance requirement can be obtained by appropriately reducing the amount of structural material and additional smart damping device.

• Archives of Pharmacal Research
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• v.21 no.4
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• pp.391-397
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• 1998

The toxicity evaluation of oriental herbal drugs is of great concern at present. Bojungchisup-tang (BCST, in Korean), a decocted medicine of oriental herbal mixture, is now well used in clinic at oriental hospitals for the treatment of edema of several diseases in practice. However, the toxicity of the oriental herbal decocted medicines such as genetic toxicity is not well defined until now. In this respect, to clarify the genetic toxicity of BCST, in vitro chromosome aberration assay with Chinese hamster lung (CHL) fibroblasts and in vivo supravital micronucleus assay with mouse peripheral reticulocytes were performed in this study. In the chromosome aberration assay, we used 5,000 $\mu\textrm{g}$/ml BCST as maximum concentration because no remarkable cytotoxicity in CHL cells was observed both in the presence and absence of S-9 metabolic activation system. No statistical significant differences of chromosome aberrations were observed in CHL cells treated with 5,000, 2,500 and 1,250 $\mu\textrm{g}$/ml BCST for 6 hour both in the presence and absence of S-9 metabolic activation. However, very weak positive result (6.5-8.0% aberration) of BCST was obtained in the absence of S-9 metabolic activation system at 5,000 $\mu\textrm{g}$/ml BCST when treated for 24 hour, i.e. 1.5 normal cell cycle time. And also, in vivo clastogenicity of BCST was studied by acridine orange-supravital staining micronucleus assay using mouse peripheral reticulocytes. We used 2,000 mg/kg as the highest oral dose in this micronucleus assay because no acute oral toxicity of BCST was observed in mice. The optimum induction time of micronucleated reticulocytes (MNRETS) was determined as 36 hours after oral administration of 2,000 mg/kg BCST. No significant differences of MNRETs between control and BCST treatment groups were observed in vivo micronucieus assay. From these results, BCST revealed very weak positive result in chromosome aberration assay in vitro with CHL cells and no clastogenicity in micronucieus assay in vivo.

• Journal of Animal Science and Technology
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• v.50 no.3
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• pp.285-292
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• 2008

Data were taken from the dairy herd improve- ment program from the year 2000, composed of 10,929 first lactation cows consisting of 290,144 test-day records and 37,723 udder type records. The objective of the study was to estimate genetic and phenotypic correlation between fore udder attachment, rear udder height, rear udder width, udder cleft, udder depth, and somatic cell score (SCS) and to calculate heritability of udder depth, front teat length and SCS in Holstein cattle in Korea. The variance component estima- tion using test day model was determined by a derivative-free algorithm-restricted maximum likeli- hood(DF-REML) analysis method. Generally phenotypic correlations were very low between udder traits and lactation SCS which varied from －0.03 to －0.06. Heritability of all type traits and SCS was smaller than 0.12. The results of this study would be applicable to SCS using linear genetic evaluation for future studies.

• Korean Journal of Food Science and Technology
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• v.34 no.4
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• pp.673-677
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• 2002

To develop a new traditional liquors using purple-fleshed sweet potato, the condition of alcohol fermentation was investigated by adding different concentrations $(5{\sim}75%)$ of cooked purple-fleshed sweet potato into mash and 10% nuruk, and fermenting for $5{\sim}15$ days. The maximum amount of ethanol (15.4%) was produced when 20% cooked purple-fleshed sweet potato and 10% nuruk were added into mash and fermented by S. cerevisiae at $25^{\circ}C$ for 15 days. The acceptability and physiological functionalities of the purple-fleshed sweet potato liquors were also investigated and compared. PSP-10 purple-fleshed sweet potato liquor prepared by adding 10% cooked purple-fleshed sweet potato into mash showed the best acceptability in the sensory evaluation test and color test $(pink{\sim}red)$; its fibrinolytic, electron-donating, and tyrosinase inhibitory activities were better than those of other purple-fleshed sweet potato liquors and wine.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.41-41
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• 2019

This study was conducted to compare the agronomic traits of well-known colored native soybean germplasms. Recently, we are increasingly interested in colored as various functional ingredient of soybeans have revealed. We used a total of 396 soybean genetic resources, consisting of ten "Seonbijabikong", 110 "Jyinunikong", 276 "Seoritaekong". We sowed on 10th June 2018 at the field of Nongsaensmyeongro in Jeonju city. The average number of days to flowering, days to maturing and days to growth of colored soybean were 53, 84 and 136 days, respectively. Days to flowering of "Seonbijabikong" were ranged from 41 to 50 days with an average 48 days, those of "Jyinunikong" were ranged from 39 to 72 days with an average 52 days. Days to flowering of "Seoritaekong" were ranged from 35 to 63 days with an average 54 days, which were earlier in "Seonbijabikong", and similar with "Seoritaekong" and "Jyinunikong". Days to growth of "Seonbijabikong" were ranged from 125 to 137 days with an average 132 days, those of "Jyinunikong" were ranged from 91 to 144 days with an average 130 days and those of "Seoritaekong" were ranged from 99 to 150 days with an average 139 days. they were shortest in "Jyinunikong" and longest in "Seoritaekong". The distribution of maturity period was from 6th September to 5th November. The maturity period was as early as September and yields were more than 100g per plant, which were all three accessions(IT161905, IT162602, IT269617), "Seoritaekong". They would be useful as breeding materials of colored soybean with early maturity. The 100-seed weight is important characteristics that distinguish the usage of soybeans. "Seoritaekong" and "Seonbijabikong" have large seed characteristics for cooking with rice, "Jyinunikong" has small seed it for medicine. The average 100 seed weight was 35.0g of "Seonbijabikong", 30.8g of "Seoritaekong" and 13.4g of "Jyinunikong", respectively. As for seed coat lust, the ratio of dull was as high as 100% of "Seonbijabikong" and 91% of "Seoritaekong", that of shiny was as high as 77% of "Jyinunikong". Cotyledon color of "Seonbijabikong" were all yellow, that of "Seoritaekong" were 94% of green. The other name of "Seoritaekong" is "Sokcheong", which means that cotyledon color is green. Therefore if cotyledon color of Seoritaekong is not green, it might be misidentified. In the future, we will increase the utilization through evaluation of functional component such as isoflavones and anthocyanins of colored soybean landraces.

• Clinical and Experimental Pediatrics
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• v.61 no.12
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• pp.403-406
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• 2018

Floating-Harbor syndrome is a rare autosomal dominant genetic disorder associated with SRCAP mutation. To date, approximately 50 cases of Floating-Harbor syndrome have been reported, but none have been reported in Korea yet. Floating-Harbor syndrome is characterized by delayed bony maturation, unique facial features, and language impairment. Here, we present a 6-year-old boy with a triangular face, deep-set protruding eyes, low-set ears, wide nose with narrow nasal bridge, short philtrum, long thin lips, clinodactyly, and developmental delay that was transferred to our pediatric clinic for genetic evaluation. He showed progressive delay in the area of language and cognition-adaption as he grew. He had previously undergone chromosomal analysis at another hospital due to his language delay, but his karyotype was normal. We performed targeted exome sequencing, considering several syndromes with similar phenotypes. Library preparation was performed with the TruSight One sequencing panel, which enriches the sample for about 4,800 genes of clinical relevance. Massively parallel sequencing was conducted with NextSeq. An identified variant was confirmed by Sanger sequencing of the patient and his parents. Finally, the patient was confirmed as the first Korean case of Floating-Harbor syndrome with a novel SRCAP (Snf2 related CREBBP activator protein) mutation (c.7732dupT, p.Ser2578Phefs*6), resulting in early termination of the protein; it was not found in either of his healthy parents or a control population. To our knowledge, this is the first study to describe a boy with Floating-Harbor syndrome with a novel SRCAP mutation diagnosed by targeted exome sequencing in Korea.

• Korean Journal of Plant Taxonomy
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• v.49 no.2
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• pp.118-126
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• 2019

The taxonomic rank of the tiny-leaved terrestrial orchid Cephalanthera subaphylla Miyabe & $Kud{\hat{o}}$ has been somewhat controversial, as it has been treated as a species or as an infraspecific taxon, under C. erecta (Thunb.) Blume [C. erecta var. subaphylla (Miyabe & $Kud{\hat{o}}$) Ohwi and C. erecta f. subaphylla (Miyabe & $Kud{\hat{o}}$) M. Hiro]. Allozyme markers, traditionally employed for delimiting species boundaries, are used here to gain information for determining the taxonomic status of C. subaphylla. To do this, we sampled three populations of five taxa (a total of 15 populations) of Cephalanthera native to the Korean Peninsula [C. erecta, C. falcata (Thunb.) Blume, C. longibracteata Blume, C. longifolia (L.) Fritsch, and C. subaphylla]. Among 20 putative loci resolved, three were monomorphic (Dia-2, Pgi-1, and Tpi-1) across the five species. Apart from C. longibracteata, there was no allozyme variation within the remaining four species. Of the 51 alleles harbored by these 17 polymorphic loci, each of the 27 alleles at 14 loci was unique to a single species. Accordingly, we found low average values of Nei's genetic identities (I) between ten species pairs (from I = 0.250 for C. erecta versus C. longifolia to I = 0.603 for C. falcata vs. C. longibracteata), with C. subaphylla being genetically clearly differentiated from the other species (from I = 0.349 for C. subaphylla vs. C. longifolia to 0.400 for C. subaphylla vs. C. falcata). These results clearly indicate that C. subaphylla is not genetically related to any of the other taxa of Cephalanthera that are native to the Korean Peninsula, including C. erecta. In a principal coordinate analysis (PCoA), C. subaphylla was positioned distant not only from C. falcata, C. longibracteata, and C. longifolia, but also from C. erecta. Finally, K = 5 was the best clustering scheme using a Bayesian approach, with five clusters precisely corresponding to the five taxa. Thus, our allozyme results strongly suggest that C. subaphylla merits the rank of species.