• Journal of Plant Biotechnology
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• 제39권1호
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• pp.1-12
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• 2012

With the increasing advances in Brassicaceae genetics and genomics, considerable progress has been made in the transformation of Brassicaceae. Transformation technologies are now being exploited routinely to determine the gene function and contribute to the development of novel enhanced crops. $Agrobacterium$-mediated transformation remains the most widely used approach for the introduction of transgenes into Brassicaceae. In $Brassica$, the transformation relies mainly on $in$ $vitro$ transformation methods. Nevertheless, despite the significant progress made towards enhancing the transformation efficiencies, some genotypes remain recalcitrant to transformation. Advances in our understanding of the genetics behind various transformations have enabled researchers to identify more readily transformable genotypes for use in routine high-throughput systems. These developments have opened up exciting new avenues to exploit model $Brassica$ genotypes as resources for understanding the gene function in complex genomes. Although many other Brassicaceae have served as model species for improving plant transformation systems, this paper summarizes on the recent technologies employed in the transformation of both $Arabidopsis$ and $Brassica$. The use of transformation technologies for the introduction of desirable traits and a comparative analysis of these as well as their future prospects are also important parts of the current research that is reviewed.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2005년도 춘계학술대회 논문집
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• pp.2015-2018
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• 2005

In this paper, we are willing to prepare the reasonable optimization, Combinatorial Optimization and Genetic Algorithm. Thus we define position status of end-effect (or terminative link module) using promised form, (G, M(G), A(G), and so on.). For this preparing step, the reorganizing procedure of Link and Joint Module is necessary, like as enumerating the kinematically identical assembly group of several links and joints. Thus, we draw a G, directed graph in a first step. Because, directed graph contains the path information between adjacent Link Module and Joint Module. From the directed graph,G, we can incite the Incidence Matrix, M(G). The incidence matrix, M(G), contains the contact information of the Link (Joint) Module and the type of Link (Joint). At the end of this paper, we generalize the modular information as a matrix form, A(G). From this matrix, we can make a population of assembly status. That is the finial output of this paper.

• Communications for Statistical Applications and Methods
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• 제19권1호
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• pp.65-75
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• 2012

실험영역을 벗어난 점에서의 분산을 최소화 하는 외삽-최적의 문제는 모형의 불확실성을 내재하고 있다. 즉, 실험영역을 벗어나게 되면 모형의 불확실성이 높아지므로 모형의 타당성 여부를 진단하여야 한다. 본 연구에서는 외삽(extrapolation) 문제하에서 기본적인 3가지 실험, 즉 제약 실험과 최대최소 실험, 그리고 복합실험 등을 융합한 새로운 제약조건형의 실험 2가지를 제안하였다. 그리고 실험영역의 바깥에 위치한 점의 위치가 실험영역을 얼마나 벗어나느냐에 따른 실험결과에 대한 영향력도 고려하여 보았다. 문제의 특성상 유전 알고리즘을 이용하여 해를 구하였다.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2004년도 추계학술대회 논문집
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• pp.818-821
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• 2004

With a rapid development in the area of micro and ultra precision technology, the micro surface machining of small size parts are explosively increased. Especially, to improve efficiency of various beams in lens and reflector, non-rotational symmetric form and several mm level heights changeable surface can be machined at a time. These geometric complex 3D surface cannot be machined by general short stroke FTS. The long stroke FTS if firmly needed to move directly several mm and have nm level positioning accuracy for the complex surface form. The long stroke FTS used linear motors to drive moving unit long and fine, aero static bearings to decrease friction and moving errors in guide way, optical linear scale with nm level resolution to measure position of FTS. Furthermore, to increase the performance of acceleration of FTS, the light material, such as AL is used for the structure and the high stiffness box type structure is selected. In this paper, the genetic algorithm approach is described to determine a set of design parameters for auto tuning. The authors have attempted to model the design problem with the objective of minimizing the error, such as variable pattern change. This method can give the better alternative than existing other method.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1995년도 식물학심포지움 식물로부터 유용 2차대사산물의 생산 PRODUCTION OF USEFUL SECONDARY METABOLITES FROM PLANTS
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• pp.40-56
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• 1995

During the past two decades, China has seen her great progress in plant biotechnology. Since the Chinese market of herb medicine is huge, while the plant resources are shrinking, particular emphasis has been placed in plant tissue and cell cultures of medicinal plants, this includes fast propagation, protoplast isolation and regeneration, cell suspension cultures and large scale fermentation. To optimize culture conditions for producing secondary compounds in vitro, various media, additives and elicitors have been tested. Successful examples of large scale culture for the secondary metabolite biosynthesis are quite limited : Lithospermum ery throrhizon and Arnebia euchroma for shikonin derivatives, Panax ginseng, P. notoginseng, P. quinquefolium for saponins, and a few other medicinal plants. Recent development of genetic transformation systems of plant cells offered a new approach to in vitro production of secondary compounds. Hairy root induction and cultures, by using Ri-plasmid, have been reported from a number of medicinal plant species, such as Artemisia annua that produces little artemisinin in normal cultured cells, and from Glycyrrhiza uralensis. In the coming five years, Chinese scientists will continue their work on large scale cell cultures of a few of selected plant species, including Taxus spp. and A. annua, for the production of secondary metabolites with medicinal interests, one or two groups of scientists will be engaged in molecular cloning of the key enzymes in plant secondary metabolism.

• 대한산업공학회지
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• 제31권4호
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• pp.303-315
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• 2005

In this paper we address a design problem for hub-and-spoke networks and then consider a capacitated hub locations problem with direct shipment (CHLPwD). We determine the location of hubs, the allocation of nodes to hubs, and direct shipment paths in the network, with the objective of minimizing the total cost in the network. In this paper, CHLPwD is formulated as 0-1 integer programming. We develop an evolutionary algorithm here to solve the large sized CHLPwD. To do this, we present the representation and the genetic operators suitable for the problem and propose a heuristic method for the allocation of nodes to hubs. To enhance the search capability, problem-specific information is used in our evolutionary algorithm. The proposed algorithm is compared with the heuristic method in terms of solution quality and computation time. The experimental results show that our algorithm can provide better solutions than the heuristic.

• 한국항해항만학회:학술대회논문집
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• 한국항해항만학회 2000년도 추계학술대회논문집
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• pp.131-142
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• 2000

Facility layout is the early stage of system design that requires a mid-term or long-term plan. Since improper facility layout might incur substantial logistics cost including material handling and re-installment costs, due consideration must be given to decisions on facility layout. Facility layout is concerned with low to arrange equipment necessary for production in a given space. Its objective is to minimize the sum of all the products of each equipment's amount of flow multiplied by distance. Facility layout also is related to the issue of NP-complete, i.e., calculated amounts exponentially increase with the increase of the number of equipment. This study discusses Hybrid GA developed, as an algorithm for facility layout, to solve the above-mentioned problems. The algorithm, which is designed to efficiently place equipment, automatically produces a horizontal passageway by the block, if a designer provides the width and length of the space to be handled. In addition, this study demonstrates the validity of the Algorithm by comparing with existing algorithms that have been developed. We present a Hybrid GA approach to the facility layout problem that improves on existing work in terms of solution quality and method. Experimental results show that the proposed algorithm is able to produce better solution quality and more practical layouts than the ones obtained by applying existing algorithms.

• 생물정신의학
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• 제10권1호
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• pp.3-19
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• 2003

The use of atypical antipsychotics is limited by occurrence of adverse reactions such as weight gain, despite of their benefits. This article provides a comprehensive review and discussion of the most significant findings regarding obesity-related pathways and integrates these with the known mechanism of atypical antipsychotic action. The focus of this article is primarily on the genetics of obesity related pathways that may be disrupted by atypical antipsychotics. This review also discussed weight gain, hyperglycemia or occurrence of diabetes while being treated with atypical antipsychotics from the point of view of pharmacogenetics. Pharmacogenetic research seeks to uncover genetic factors that will help clinicians identify the best treatment strategies for their patients. It will aid clinically in the prediction of response and side effects, such as antipsychotic-induced weight gain, and minimize the current "trial and error" approach to prescribing in the near future. This article also presents the genetics of both central and peripheral pathways putatively involved in antipsychotic-induced weight gain while providing a comprehensive review of the obesity literature. This article also review obesity related candidate molecules which may be disrupted during atypical antipsychotic drug treatment.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2002년도 추계학술대회논문집
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• pp.422-427
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• 2002

This paper focuses on the formulation and validation of an automatic strategy for the selection of the locations and directions of strain gauges to capture at best the modal response of a blade in a series of modes. These locations and directions are selected to render the strain measurements as robust as possible with respect to random mispositioning of the gauges and gauge failures. The approach relies on the evaluation of the signal-to-noise ratios of the gauge measurements from finite element strain data and includes the effects of gauge size. A genetic algorithm is used to find the strain gauge locations-directions that lead to the largest possible value of the smallest modal strain signal-to-noise ratio, in the absence of gauge failure, or of its expected value when gauge failure is possible. A fan blade is used to exemplify the applicability of the proposed methodology and to demonstrate the effects of the essential parameters of the problem, i.e. the mispositioning level, the probability of gauge failure, and the number of gauges.

• Toxicological Research
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• 제25권2호
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• pp.85-92
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• 2009

4,4'-Methylenedianiline (MDA) is an aromatic amine that is widely used in the industrial synthetic process. Genotoxic MDA forms DNA adducts in the liver and is known to induce liver damage in human and rats. To elucidate the molecular mechanisms associated with MDA-induced hepatotoxicity, we have identified genes differentially expressed by microarray approach. BALB/c male mice were treated once daily with MDA (20 mg/kg) up to 7 days via intraperitoneal injection (i.p.) and hepatic damages were revealed by histopathological observation and elevation of serum marker enzymes such as AST, ALT, ALP, cholesterol, DBIL, and TBIL. Microarray analysis showed that 952 genes were differentially expressed in the liver of MDA-treated mice and their biological functions and canonical pathways were further analyzed using Ingenuity Pathways Analysis (IPA). Toxicological functional analysis showed that genes related to hepatotoxicity such hyperplasia/hyperproliferation (Timp1), necrosis/cell death (Cd14, Mt1f, Timp1, and Pmaip1), hemorrhaging (Mt1f), cholestasis (Akr1c3, Hpx, and Slc10a2), and inflammation (Cd14 and Hpx) were differentially expressed in MDA-treated group. This gene expression profiling should be useful for elucidating the genetic events associated with aromatic amine-induced hepatotoxicity and for discovering the potential biomarkers for hepatotoxicity.