• Asian Pacific Journal of Cancer Prevention
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• 제17권3호
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• pp.1221-1233
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• 2016

Purpose: To investigate any potential association between wine and breast cancer risk. Materials and Methods: We quantitatively assessed associations by conducting a meta-analysis based on evidence from observational studies. In May 2014, we performed electronic searches in PubMed, EmBase and the Cochrane Library to identify studies examining the effect of wine drinking on breast cancer incidence. The relative risk (RR) or odds ratio (OR) were used to measure any such association. Results: The analysis was further stratified by confounding factors that could influence the results. A total of twenty-six studies (eight case-control and eighteen cohort studies) involving 21,149 cases were included in our meta-analysis. Our study demonstrated that wine drinking was associated with breast cancer risk. A 36% increase in breast cancer risk was observed across overall studies based on the highest versus lowest model, with a combined RR of 1.0059 (95%CI 0.97-1.05) in dose-response analysis. However, 5 g/d ethanol from wine seemed to have protective value from our non-linear model. Conclusions: Our findings indicate that wine drinking is associated with breast cancer risk in a dose-dependent manner. High consumption of wine contributes to breast cancer risk with protection exerted by low doses. Further investigations are needed for clarification.

• Asian Pacific Journal of Cancer Prevention
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• 제15권17호
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• pp.7169-7174
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• 2014

Background: NPAS2 is a product of the circadian clock gene. It acts as a putative tumor suppressor by playing an important role in DNA damage responses, cell cycle control and apoptosis. Chronic lymphocytic leukemia (CLL) appears to be an apoptosis related disorder and alteration in the NPAS2 gene might therefore be directly involved in the etiology of CLL. Here, the Ala394Thr polymorphism (rs2305160:G>A) in the NPAS2 gene was genotyped and melatonin concentrations were measured in a total of seventy-four individuals, including thirty-seven CLL cases and an equal number of age- and sex-matched healthy controls in order to examine the effect of NPAS2 polymorphism and melatonin concentrations on CLL risk in a Pakistani population. Materials and Methods: Genotyping of rs2305160:G>A polymorphism at NPAS2 locus was carried out by amplification refractory mutation system-polymerase chain reaction (ARMS-PCR). Melatonin concentrations were determined by enzyme linked immunosorbent assay (ELISA). Statistical analysis was performed using Statistical Package for Social Sciences software. Results: Our results demonstrated no association of the variant Thr genotypes (Ala/Thr and Thr/Thr) with risk of CLL. Similarly, no association of rs2305160 with CLL was observed in either females or males after stratification of study population on a gender basis. Moreover, when the subjects with CLL were further stratified into shift-workers and non-shift-workers, no association of rs2305160 with CLL was seen in either case. However, significantly low serum melatonin levels were observed in CLL patients as compared to healthy subjects (p<0.05). Also, lower melatonin levels were seen in shift-workers as compared to non-shift-workers (p<0.05). There was no significant difference (p>0.05) in the melatonin levels across NPAS2 genotypes in all subjects, subjects with CLL who were either shift workers or non-shift-workers. General Linear Model (GLM) univariate analysis revealed no significant association (p>0.05) of the rs2305160 polymorphism of the NPAS2 gene with melatonin levels in any of the groups. Conclusions: While low melatonin levels and shift-work can be considered as one of the risk factors for CLL, the NPAS2 rs2305160 polymorphism does not appear to have any association with risk of CLL in our Pakistani population.

• 사상체질의학회지
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• 제21권3호
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• pp.48-62
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• 2009

1. Objectives: The purpose of this study was to analyze the physical and psychological characteristics of each Sasang type groups which were classified by Sasang medicine specialist. 2. Methods: The subjects were 115 (85.2% of male) university students with $26.8\pm5.4$ yrs. of age and $12.9\pm1.7$ yrs. of education. We can get 30 of Soyang type, 39 of Taeeum type, 46 of Soeum type after the Sasang type classification. Their physical characteristics were measured with Bioelectrical Impedance Analysis (Inbody 720, Biospace, Seoul), and psychological characteristics with Temperament and Character Inventory (Maumsarang, Seoul). The general linear model (SPSS 12.0, SPSS Inc, Chicago) were used for the analysis with gender and age as covariance. 3. Results and Conclusions: Taeeum type group ($75.64\pm13.01$, $26.12\pm4.16$, $15.74\pm2.42$, $11.30\pm1.80$, $3.93\pm0.62$, $18.43\pm7.70$) showed significantly bigger weight, intra-cellular water, extra-cellular water, protein, mineral, body fat mass than Soeum type group ($61.32\pm8.56$, $22.54\pm3.87$, $13.70\pm2.15$, $9.74\pm1.68$, $3.35\pm0.51$, $10.79\pm3.97$). In obesity degree such as Body Mass Index and Waist-hip ratio, and circumference measures in chest, waist and hip, we found significant differences in the order of Taeeum>Soyang>Soeum. As a psychological perspectives Soyang type group ($41.90\pm13.32$) has higher NS (Novelty-Seeking) score than Soeum type group ($35.22\pm11.22$) with marginal significance (p=0.052). In sub-scale measurements Soeum type group ($10.61\pm3.69$) has significantly lower value than other two groups in P3 (Ambition). The results of present study showed that Taeeum type by specialist has same physical characteristics as previously reported QSCC II based studies. Temperament profile of each Sasang type group will serve as an important evidence for further study showing that the Sasang typology has biological basis.

• 한국치위생학회지
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• 제15권3호
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• pp.487-495
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• 2015

Objectives: The purpose of the study was to investigate the associated factors with oral health behavior in public health majoring students. Methods: Using convenience sampling method, the subjects were 474 health-related majoring students in Jeollanamdo. A self-reported questionnaire was completed from September 1 to 15, 2014. The questionnaire consisted of general characteristics of the subjects, oral health related characteristics, oral health knowledge and behavior. Data were analyzed using SAS 9.3 version. T-test, ANOVA, correlation and multiple linear regression analyses were performed to evaluate the related factors with oral health behavior. Cronbach's ${\alpha}$ in oral health knowledge in this study was 0.52 and that in oral health behavior was 0.80. Results: The overall score of oral health behavior was $3.38{\pm}0.52$. Of all behaviors, the practice in brush of teeth and tongue had the highest mean score. In multiple regression analysis, oral health knowledge in the nursing and dental hygiene students was positively associated with the oral health behavior(${\beta}=0.04$, p=0.003, ${\beta}=0.23$, p=0.003, and ${\beta}=0.18$, p=0.034, respectively). Necessity of dental care, one of oral symptom, and more than two oral symptoms were negatively associated with oral health behavior(${\beta}=-0.14$, p=0.002, ${\beta}=-0.11$, p=0.037, and ${\beta}=-0.17$, p=0.011, respectively). Conclusions: Higher oral health knowledge showed higher levels of oral health behavior. These results will enhance the quality of oral health behavior by increasing the level of oral health knowledge. The optimal oral health education program would be able to improve oral health behavior by increasing the level of oral health knowledge.

• Asian-Australasian Journal of Animal Sciences
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• 제31권10호
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• pp.1565-1574
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• 2018

Objective: The aim of the present study was to identify genetic variants based on RNA-seq data, obtained via transcriptome sequencing of muscle tissue of pigs differing in muscle histological structure, and to verify the variants' effect on histological microstructure and production traits in a larger pig population. Methods: RNA-seq data was used to identify the panel of single nucleotide polymorphisms (SNPs) significantly related with percentage and diameter of each fiber type (I, IIA, IIB). Detected polymorphisms were mapped to quantitative trait loci (QTLs) regions. Next, the association study was performed on 944 animals representing five breeds (Landrace, Large White, Pietrain, Duroc, and native Puławska breed) in order to evaluate the relationship of selected SNPs and histological characteristics, meat quality and carcasses traits. Results: Mapping of detected genetic variants to QTL regions showed that chromosome 14 was the most overrepresented with the identification of four QTLs related to percentage of fiber types I and IIA. The association study performed on a 293 longissimus muscle samples confirmed a significant positive effect of transforming acidic coiled-coil-containing protein 2 (TACC2) polymorphisms on fiber diameter, while SNP within forkhead box O1 (FOXO1) locus was associated with decrease of diameter of fiber types IIA and IIB. Moreover, subsequent general linear model analysis showed significant relationship of FOXO1, delta 4-desaturase, sphingolipid 1 (DEGS1), and troponin T2 (TNNT2) genes with loin 'eye' area, FOXO1 with loin weight, as well as FOXO1 and TACC2 with lean meat percentage. Furthermore, the intramuscular fat content was positively associated (p<0.01) with occurrence of polymorphisms within DEGS1, TNNT2 genes and negatively with occurrence of TACC2 polymorphism. Conclusion: This study's results indicate that the SNP calling analysis based on RNA-seq data can be used to search candidate genes and establish the genetic basis of phenotypic traits. The presented results can be used for future studies evaluating the use of selected SNPs as genetic markers related to muscle histological profile and production traits in pig breeding.

• Asian-Australasian Journal of Animal Sciences
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• 제31권4호
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• pp.480-488
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• 2018

Objective: Average daily gain (ADG) is an important target trait of pig breeding programs. We aimed to identify single nucleotide polymorphisms (SNPs) and genomic regions that are associated with ADG in the Duroc pig population. Methods: We performed a genome-wide association study involving 390 Duroc boars and by using the PorcineSNP60K Beadchip and two linear models. Results: After quality control, we detected 3,5971 SNPs, which included seven SNPs that are significantly associated with the ADG of pigs. We identified six quantitative trait loci (QTL) regions for ADG. These QTLs included four previously reported QTLs on Sus scrofa chromosome (SSC) 1, SSC5, SSC9, and SSC13, as well as two novel QTLs on SSC6 and SSC16. In addition, we selected six candidate genes (general transcription factor 3C polypeptide 5, high mobility group AT-hook 2, nicotinamide phosphoribosyltransferase, oligodendrocyte transcription factor 1, pleckstrin homology and RhoGEF domain containing G4B, and ENSSSCG00000031548) associated with ADG on the basis of their physiological roles and positional information. These candidate genes are involved in skeletal muscle cell differentiation, diet-induced obesity, and nervous system development. Conclusion: This study contributes to the identification of the casual mutation that underlies QTLs associated with ADG and to future pig breeding programs based on marker-assisted selection. Further studies are needed to elucidate the role of the identified candidate genes in the physiological processes involved in ADG regulation.

• Asian Pacific Journal of Cancer Prevention
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• 제14권2호
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• pp.997-1001
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• 2013

Background: Lymphangiogenesis, assessed as lymphovascular density (LVD), is the initial step of generalized tumor lymphovascular invasion (LVI). It also involves VEGF-C as the most important protein family. Lymphangiogenesis among breast cancer cases correlations with several clinicopathological factors are important to determine prognosis and treatment strategies, but results have been controversial and require clarification. Aim: To define correlations between VEGF-C expression, LVD and LVI with several clinicopathological parameters from Indonesian breast cancer patients. Materials and Methods: Using a cross-sectional study, a total of 48 paraffin-embedded tissues of breast cancer from Dr. Sardjito General Hospital Indonesia were assessed for VEGF-C expression, LVD and LVI by immunohistochemistry. Correlations of these markers with clinicopathological parameters like patient age, tumor size, lymph node status, grade, ER/PR and Her-2 status, cell proliferation and p-53 expression were investigated by linear analysis. Correlations of VEGF-C expression and LVI with several clinicopathological parameters were analyzed with Coefficient Contingency Chi-Square test. Results: The mean of patients age was 53.0 year, pre and post-menopausal patients accounting for 56.3% and 43.8%, respectively. Some 10.4% were well, 41.7% moderate and 47.9% poorly differentiated. ER positivity was evident in 50% while PR and Her-2 positivity was found in 31.3% and 33.3%, respectively. Breast cancer cells with over-expression of p-53 was 64.6% and with high cell proliferation was 56.3%. Lymph node metastasis was noted in 63.5%, and LVI in 72.9%. Significant correlations were found between LVD and tumor size (p:0.037), grade (p:0.000), lymphnode status (p:0.036), LVI (p:0.003), as well as with p-53 and cell proliferation. There were also significant correlation of VEGF-C (p:0.011) and LVI (p:0.001) with tumor grade. Only ER status was found to have a correlation with tumor size (p:0.027). Conclusions: This study suggested that in Indonesian breast cancer patients, lymphangiogenesis is correlated with tumor size, grade, lymph node status and tumor lymphovascular invasion, the latter also being related with p-53 over expression and high cell proliferation.

• 한국통신학회논문지
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• 제25권5A호
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• pp.717-725
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• 2000

최근 수년간 얼굴인식에 관한 많은 알고리즘이 개발되었고 그 대다수가 view와 투사에 기초한 알고리즘이었다. 본 논문에서의 투사는 비단 직교 기저상에 영상을 투사하는 것으로 국한하지 않고 영상 화소값을 변환하는 일반적인 선형 변환으로써 상관관계, 주성분 분석, 클러스트링, gray scale 투사, 그리고 추적 필터매칭을 포함한다. 본 연구에서는 FERET 데이터베이스 상의 얼굴 영상을 평가한 알고리즘들을 세부적으로 분석하고자 한다. 투사에 기초한 알고리즘은 3단계로 구성된다. 첫 번째 단계는 off-line상에서 행하며 알고리즘 설계자에 의해 새로운 기저가 설정되거나 또는 학습을 통해 새로운 기저를 결정한다. 두 번째 단계는 on-line상에서 행해지며 영상을 설정된 새로운 기저상에 투사한다. 세 번째 단계는 on-line상에서 행해지며 영상내의 얼굴은 가장 인접한 이웃 분류자로 인식된다. 대부분의 평가 방법들은 단일 gallery 상에서의 성능 평가가 이루어짐으로써 알고리즘 성능을 충분히 측정하지 못하는 반면 본 연구에서는 독립된 galley들의 집합을 구성함으로써 각각의 다른 galley상에서 가지는 변화와 이들의 상대적 성능을 평가한\ulcorner.

• 대한지역사회영양학회지
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• 제19권6호
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• pp.537-549
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• 2014

Objectives: This study was conducted to examine blood pressure and other characteristics of a high sodium intake group assessed with 24-hr urine analysis and the dietary factors related to the risk of high sodium intake among Korean adults. Methods: A cross-sectional study was conducted with adults aged 20-59 years. Subjects who completed 24-hr urine collection (N = 205) were divided into 3 groups (tertile) according to the sodium intake estimated with 24-hour urine analysis. We compared the blood pressure, BMI and dietary related factors of the 3 groups (low, medium, high sodium intake group) with General Linear Model (GLM) and Duncan's multiple range test (p < 0.05). The risk factors related to high sodium intake were assessed with odds ratio (p < 0.05). Results: The sodium intake (mg/day) of the 3 groups were $3359.8{\pm}627.9$, $4900.3{\pm}395.1$ and $6770.6{\pm}873.9$, respectively, corresponding to daily salt intake (g/day) 8.5, 12.4 and 17.2, respectively. High sodium group showed significantly elevated age, BMI and systolic/diastolic blood pressure. Being male gender was associated with significantly increased risk of sodium intake (OR = 1.972; 95%CI: 1.083-3.593). The other factors related to high sodium intake were higher BMI (${\leq}25$) (OR = 2.619; 95% CI: 1.368-5.015), current alcohol consumption (OR = 1.943; 95%CI: 1.060-3.564), and having salty soybean paste with salt percentage > 14% (OR = 3.99; 95% CI: 1.404-6.841). The dietary attitude related to increased risk of high sodium intake included 'enjoy dried fish and salted mackerel' (p < 0.001) and 'eat all broth of soup, stew or noodle' (p < 0.001). Conclusions: Because high sodium intake was associated with higher blood pressure, nutrition education should focus on alcohol consumption, emphasis on related dietary factors such as using low salt soybean paste, improvements in the habit of eating dried fish and salted mackerel or eating all broth of soup, stew or noodle.

• Asian-Australasian Journal of Animal Sciences
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• 제31권8호
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• pp.1119-1126
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• 2018

Objective: Present investigation was aimed to study the Single Nucleotide Variants of the luteinizing hormone beta ($LH{\beta}$) gene and to analyze their association with the semen quality (fresh and post-thawed frozen semen) and luteinizing hormone (LH) concentrations in Murrah buffalo bulls. Methods: Polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) and Sanger sequencing method is used to study genetic variability in $LH{\beta}$ gene. LH assay was carried out using enzyme-linked immunosorbent assay method. A fixed general linear model was used to analyze association of single nucleotide polymorphism (SNP) of $LH{\beta}$ gene with semen quality in 109 and LH concentrations in 80 Murrah bulls. Results: $LH{\beta}$ gene was found to be polymorphic. Total six SNPs were identified in $LH{\beta}$ gene g C356090A, g C356113T, g A356701G, g G355869A, g G356330C, and g G356606T. Single Stranded Conformational Polymorphism variants of pattern 2 of exon 1+pattern 2 of exon 2+pattern 1 of exon 3 had highly significant (p<0.01) effect on sperm concentration (million/mL), percent mass motility, acrosome integrity and membrane integrity in fresh and frozen semen whereas significant (p<0.05) effect was observed on percent live spermatozoa. SSCP variants of pattern 2 of exon 1+pattern 2 of exon 2+pattern 1 of exon 3 had highly significant (p<0.01) effect on luteinizing hormone concentrations too. Conclusion: The observed association between SSCP variants of $LH{\beta}$ gene with semen quality parameters and LH concentrations indicated the possibilities of using $LH{\beta}$ as a candidate gene for identification of markers for semen quality traits and LH concentrations in Murrah buffaloes.