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Construction of Infectious cDNA Clone of a Chrysanthemum stunt viroid Korean Isolate

  • Yoon, Ju-Yeon;Cho, In-Sook;Choi, Gug-Seoun;Choi, Seung-Kook
    • The Plant Pathology Journal
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    • v.30 no.1
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    • pp.68-74
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    • 2014
  • Chrysanthemum stunt viroid (CSVd), a noncoding infectious RNA molecule, causes seriously economic losses of chrysanthemum for 3 or 4 years after its first infection. Monomeric cDNA clones of CSVd isolate SK1 (CSVd-SK1) were constructed in the plasmids pGEM-T easy vector and pUC19 vector. Linear positive-sense transcripts synthesized in vitro from the full-length monomeric cDNA clones of CSVd-SK1 could infect systemically tomato seedlings and chrysanthemum plants, suggesting that the linear CSVd RNA transcribed from the cDNA clones could be replicated as efficiently as circular CSVd in host species. However, direct inoculation of plasmid cDNA clones containing full-length monomeric cDNA of CSVd-SK1 failed to infect tomato and chrysanthemum and linear negative-sense transcripts from the plasmid DNAs were not infectious in the two plant species. The cDNA sequences of progeny viroid in systemically infected tomato and chrysanthemum showed a few substitutions at a specific nucleotide position, but there were no deletions and insertions in the sequences of the CSVd progeny from tomato and chrysanthemum plants.

Effects of Heavy Metals on Biomonitoring using Recombinant Bioluminescence Bacteria (유전자재조합균주를 이용한 생물모니터링에 중금속 오염물이 미치는 영향 평가)

  • Kong, In Chul;Kim, Jin Young;Ko, Kyung-Seok
    • Journal of Soil and Groundwater Environment
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    • v.18 no.4
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    • pp.32-39
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    • 2013
  • This research focused on the effects of heavy metals on the biomonitoring activity of genetically engineered bioluminescent bacteria, Pseudomonas putida mt-2 KG1206. KG1206 was exposed to single or binary mixtures of different heavy metals as well as soils contaminated with heavy metals. In case of single exposure with different inducer pollutant, the toxicity order was as followings : As(III) > Cd, As(V) >> Cu, Cr(VI). The toxic effects of the binary mixtures was compared to the expected effect based on a simple theory of probabilities. The interactive effects were mostly additive, while in few cases antgonistic and synergistic mode of action was observed for some concentration combinations. No considerable correlation was found between the total metal contents in soils and the bioluminescence activity of each sample. However, statistically significant differences (p = 0.0102) were observed between two groups, classified based on arsenite contamination. These results demonstrate the usage of recombinant bioluminescent microorganism in biomonitoring and the complex interactive effects of metals.

Synthesis of Zr0.73Y0.27O1.87 Crystals by the Bridgman-Stockbager Method

  • Kim, Won-Sa;Yu, Young-Moon;Lee, Jin-Ho
    • Journal of the Korean earth science society
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    • v.23 no.1
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    • pp.52-58
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    • 2002
  • A colorless and transparent zirconium oxide ($Zr_{0.73}Y_{0.27}O_{1.87}$) crystal has been synthesized by the Bridgman-Stockbager method. The gem-quality material is produced by adding 20${\sim}$25 wt.% $Y_2O_3$ (stabilizer) and 0.04 wt.% $Nd_2O_3$ (decolorising agent) to the $ZrO_2$ powder. It shows a vitreous luster with a slight oily appearance. Under a polarizing microscope, it shows isotropic nature with no appreciable anisotropism. Mohs hardness value and specific gravity is measured to be 8${\sim}$$8{\frac{1}{2}}$ and 5.85, respectively. Under ultraviolet light it shows a faint white glow. The crystal structure of yttria-stabilized zirconia with 0.27 at.% Y has been re-investigated, using single crystal X-ray diffraction, and confirmed to be a cubic symmetry, space group $Fm{\overline{3}}m$ ($O^5_h$) with a=5.1552(5) ${{\AA}}$, V=136.99(5) ${{\AA}}^3$, Z=4. The stabilizer atoms randomly occupy the zirconium sites and there are displacements of oxygen atoms with amplitudes of ${\Delta}/a{\sim}$0.033 and 0.11 along <110> and <111> from the ideal positions of the fluorite structure, respectively.

Building an Ensemble Machine by Constructive Selective Learning Neural Networks (건설적 선택학습 신경망을 이용한 앙상블 머신의 구축)

  • Kim, Seok-Jun;Jang, Byeong-Tak
    • Journal of KIISE:Software and Applications
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    • v.27 no.12
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    • pp.1202-1210
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    • 2000
  • 본 논문에서는 효과적인 앙상블 머신의 구축을 위한 새로운 방안을 제시한다. 효과적인 앙상블의 구축을 위해서는 앙상블 멤버들간의 상관관계가 아주 낮아야 하며 또한 각 앙상블 멤버들은 전체 문제를 어느 정도는 정확하게 학습하면서도 서로들간의 불일치 하는 부분이 존재해야 한다는 것이 여러 논문들에 발표되었다. 본 논문에서는 주어진 문제의 다양한 면을 학습한 다수의 앙상블 후보 네트웍을 생성하기 위하여 건설적 학습 알고리즘과 능동 학습 알고리즘을 결합한 형태의 신경망 학습 알고리즘을 이용한다. 이 신경망의 학습은 최소 은닉 노드에서 최대 은닉노드까지 점진적으로 은닉노드를 늘려나감과 동시에 후보 데이타 집합에서 학습에 사용할 훈련 데이타를 점진적으로 선택해 나가면서 이루어진다. 은닉 노드의 증가시점에서 앙상블의 후부 네트웍이 생성된다. 이러한 한 차례의 학습 진행을 한 chain이라 정의한다. 다수의 chain을 통하여 다양한 형태의 네트웍 크기와 다양한 형태의 데이타 분포를 학습한 후보 내트웍들이 생성된다. 이렇게 생성된 후보 네트웍들은 확률적 비례 선택법에 의해 선택된 후 generalized ensemble method (GEM)에 의해 결합되어 최종적인 앙상블 성능을 보여준다. 제안된 알고리즘은 한개의 인공 데이타와 한 개의 실세계 데이타에 적용되었다. 실험을 통하여 제안된 알고리즘에 의해 구성된 앙상블의 최대 일반화 성능은 다른 알고리즘에 의한 그것보다 우수함을 알 수 있다.

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The Biodegradation of Mixtures of Benzene,Phenol,and Toluene by Mixed and Monoculture of Bacteria (단일배양 및 혼합배양에 의한 Benzene, Phenol 및 Toluene 혼합물의 생분해)

  • Lee, Chang-Ho;Oh, Hee-Mock;Kwon, Tae-Jong;Kwon, Gi-Seok;Kim, Seong-Bin;Kho, Yung-Hee;Yoon, Byung-Dae
    • Microbiology and Biotechnology Letters
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    • v.22 no.6
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    • pp.685-691
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    • 1994
  • The biodegradation of aromatic compounds by mixed and monoculture was investigated in an artificial wastewater containing 500 mg/l of benzene(B), phenol(P), and toluene(T) in various combinations. None of three strains utilized P-xylene(X) as a carbon source, but they grew well on p-xylene in mixtures with benzene and toluene. In the mixed culture on mixed substrate, the length of lag phase was different depending on the nature of mixture. Cell growths of Flavobac- terium sp. BEN2 and Acinetobacter sp. GEM63 were inhibited in the presence of a 500 mg/l of phenol. When the mixed culture of three strains was cultured in a bench-scale reactor containing artificial wastewater, each of benzene, phenol, and toluene was not detected at 30 hrs, 50 hrs, and 12 hrs after incubation in the treatment. The removal rates of COD$_{t}$(total COD) and COD$_{s}$,(soluble COD) of upper phase after centrifugation during early 50 hrs were ca. 80% and ca. 93.8%, respectively.

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Photoelectric Observation of the Long-Period Eclipsing Binaries at Yonsei University Observatory

  • Nha, Il-Seong;Lee, Yong-Sam;Chun, Yong-Woo;Kim, Ho-Il;Kim, Young-Soo
    • Journal of Astronomy and Space Sciences
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    • v.3 no.1
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    • pp.1-13
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    • 1986
  • A long-term project(ten-year ; 1982-92) for the photoeletric observation in the UBV passbands of selected eclipsing binaries with P$\geq$10 days has initiated at Yonsei University Observatory using 40-cm and 61-cm reflectors. the instrumentatio used and the observation techniques and the reduction procedures applied to this investiagation are described. Out of 39 program stars, successful results have been obtained during our first two years for the 11 stars listed below, with the orbital period in days in the parenthesis, and the incomplete light curves of these stars are presented. SX Cas (36.57) AQ Cas (11.72) UU Cnc (96.71) RY Gem (9.30) V373 Cas (13.42) NY Cep (15.28) RX Cas (32.32) V396 Cas (15.28) ZZ Cnc (25.60) Zet Aur (982.2) Eps Aur (9885.) For the rest of the stars, the observations made in the first two years are not sufficient to attempt any meaning light curve construction ; some of the data are too fragmented and others show large scatter.

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Analysis of Soil Properties and Microbial Communities for Mine Soil Vegetation (폐광산지역 토양 식생복원 과정 내 토양특성 및 미생물 군집 변화 분석)

  • Park, Min-Jeong;Yoon, Min-Ho;Nam, In-Hyun
    • Journal of Soil and Groundwater Environment
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    • v.20 no.3
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    • pp.83-91
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    • 2015
  • Mine soil contamination by high levels of metal ions that prevents the successful vegetation poses a serious problem. In the study presented here, we used the microbial biocatalyst of urease producing bacterium Sporosarcina pasteurii or plant extract based BioNeutro-GEM (BNG) agent. The ability of the biocatalysts to bioremediate contaminated soil from abandoned mine was examined by solid-state composting vegetation under field conditions. Treatment of mine soil with the 2 biocatalysts for 5 months resulted in pH increase and electric conductivity reduction compared to untreated control. Further analyses revealed that the microbial catalysts also promoted the root and shoot growth to the untreated control during the vegetation treatments. After the Sporosarcina pasteurii or plant extract based BNG treatment, the microbial community change was monitored by culture-independent pyrosequencing. These results demonstrate that the microbial biocatalysts could potentially be used in the soil bioremediation from mine-impacted area.

Properties of the Natural and CVD Synthetic Diamonds for Identification (천연과 CVD 합성 다이아몬드의 감별을 위한 물성 연구)

  • Kim, Yunwoo;Song, Jeongho;Noh, Yunyoung;Song, Ohsung
    • Journal of the Korean Ceramic Society
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    • v.51 no.4
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    • pp.350-356
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    • 2014
  • Recently, Chemical Vapor Deposition (CVD) synthetic diamonds have been introduced to the jewelry gem market, as CVD technology has been making considerable advances. Unfortunately, CVD diamonds are not distinguishable from natural diamonds when using the conventional gemological characterization method. Therefore, we need to develop a new identification method that is non-destructive, fast, and inexpensive. In our study, we employed optical microscopy and spectroscopy techniques, including Fourier transform infra-red (FT-IR), UV-VIS-NIR, photoluminescence (PL), micro Raman, and cathodoluminescent (CL) spectroscopy, to determine the differences between a natural diamond (0.30 cts) and a CVD diamond (0.43 cts). The identification of a CVD diamond was difficult when using standard gemological techniques, UV-VIS-NIR, or micro-Raman spectroscopy. However, a CVD diamond could be identified using a FT-IR by the Type II peaks. In addition, we identified a CVD diamond conclusively with the uneven UV fluorescent local bands, additional satellite PL peaks, longer phosphorescence life time, and uneven streaks in the CL images. Our results suggest that using FT-IR combined with UV fluorescent images, PL, and CL analysis might be an appropriate method for identifying CVD diamonds.

Antitank Mine Detection with Geophysical Prospecting (물리탐사를 이용한 대전차 지뢰 탐지)

  • Cho, Seong-Jun;Kim, Jung-ho;Son, Jeong-Sul;Bang, Eun-Seok;Kim, Jong-Wook
    • 한국지구물리탐사학회:학술대회논문집
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    • 2007.06a
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    • pp.219-224
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    • 2007
  • We conducted geophysical surveys to detect antitank mine at Namji-eup, Gyeongsangnam-do which had been installed during Korean war. The surveys consisted of 2 stages, at the first stage we divided the survey area into 7 block and carried out magnetic gradient survey and GEM-3 EM survey sequentially for each block. Hence we verified anomaly areas using an excavator and a metal detector. Most of anomalies were found to be garbages such as trash cans, metallic wastes, and so on. And also, the concrete pipe was found at depth of 1 m, which had not referred in any report of that area. At the second stage, after trenching the covered soil down to 75 cm the same surveys were conducted. We could not find the strong signal to be inferred from a antitank mine, but we pointed out some anomalies to need careful handling because demining is very dangerous work even though there is few possibility that is mine.

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Examining the Influence of Age and Gender on Entrepreneurship in VietnamI

  • DO, Quang Hung;TRAN, The Tuan
    • The Journal of Asian Finance, Economics and Business
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    • v.7 no.10
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    • pp.193-199
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    • 2020
  • This objective of this study is to examine the influence of age and gender on entrepreneurship in Vietnam's context. Although various studies have been conducted to examine age and gender differences in the entrepreneurial process, the results reveal inconsistencies. Moreover, the relationship between different factors and their role in explaining age and gender differences in the entrepreneurship activities still has no clear answer. Our analyses were based on data collected for the 2017 Global Entrepreneurship Monitors (GEM) from individuals located in Vietnam with a sample of 2,118. Our findings show that female entrepreneurs had less household income and were likely to have more fear of failure in starting a business. Entrepreneurs tend to be older and young entrepreneurs have better education. The study also indicated that male entrepreneurs were likely to have more perception of the ease to start a business. Moreover, the number of male respondents who are currently entrepreneurs is higher and they are older. The contribution of this study is twofold. First, it provides empirical evidence on the age and gender differences in entrepreneurship. Second, it contributes to the understanding of the entrepreneurship environment in Vietnam, one of the most dynamic emerging countries in the East Asian region.