• 한국작물학회지
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• 제46권1호
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• pp.12-16
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• 2001

In order to develop near isogenic lines (NILs) the heterozygous rice plants for alkali digestibility value(ADV) were selected and tested in every generation from $F_5$to $F_9$ of a cross, Seratus malam/Suwon 345. Finally several sets of NILs, which were six low ADV lines and four medium-high ADV lines, were selected among $F_{10}$ lines. No differences of the plant growth characteristics, amylose content and protein content of rice grain were found between low and medium-high ADV lines. Rice flour of low ADV lines showed longer gel length in gel consistency test than medium-high ADV lines, and also showed different gelatinization characteristics measured by rapid visco analyzer compared with medium-high ADV lines. The result of genetic analysis indicated that ADV-NILs developed were differed in a pair of major gene controlling ADV, and low ADV was dominant over medium-high ADV.

• Journal of Pharmaceutical Investigation
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• 제39권5호
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• pp.381-386
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• 2009

We previously showed that the water extracts of rice wine-baked Scutellaria baicalensis Georgi (RWBS) ameliorated colonic inflammation more than crude Scutellaria baicalensis (CS) after oral administration. The aim of this study is to compare the effect of rectal and oral administration of RWBS in the experimental colitis. Experimental colitis was induced in mice by daily treatment with 5% dextran sulfate sodium (DSS) in the drinking water for 7 days. Water was used as vehicle of oral administration, while Carbopol/PEG mucoadhesive gel was used as vehicle of rectal administration. RWBS and RWBS gel (RWBSG) were administered once per day for 7 days. RWBS and RWBSG significantly attenuated the disease activity index (DAI) calculated as the sum of scores of body weight loss, stool consistency and rectal bleeding. Furthermore, RWBS and RWBSG reduced the mucosal myeloperoxidase activity and COX-2 (cyclooxygenase-2) expression in colon tissue. Anti-inflammatory effect of CS on colonic inflammation was increased by baking with rice wine in both oral and rectal administration. Moreover, anti-inflammatory effects of oral administration on colonic inflammation was greater than those of rectal administration. Further study would be required for the development of intra-rectal formulation.

• Current Research on Agriculture and Life Sciences
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• 제29권
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• pp.1-10
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• 2011

This study was conducted to determine the agronomic characteristics of low amylose lines which were derived from induced mutants by T-DNA insertion. The agronomic and physicochemical properties of the low amylose mutants were analyzed and compared with a donor cultivar 'Dongjin' and a low amylose cultivar 'Baekjinju'. The heading date of the low amylose mutants was similar to the donor cultivar 'Dongjin' and yield index of the mutants was 78~92% compared with 'Dongjin'. The amylose content of four mutants in brown rice was ranged from 16.1 to 16.7%. Among low amylose mutants, 'P50-4-4-5' was lower 3.6%(13.3%) than those of 'Dongjin'(16.9%) in amylose content of milled rice. The grain length of 'P50-4-4-5' was similar to the donor cultivar, however, thousand grain weight(18.9g) was lighter than those of 'Dongjin'. The score of alkali digestion in brown rice of 'P50-4-4-5'(5.5) was lower than that of 'Dongjin'(6.8) and similar to 'Baekjinju'(5.8). The gel consistency of 'P50-4-4-5'(84mm) in milled rice exhibited that was longer than 'Dongjin'(76mm) and 'Baekjinju'(81mm). The result of eating quality showed that 'P50-4-4-5'(78.8) was higher than those of 'Dongjin'(60.3) and 'Baekjinju'(67.2). Thus our data suggest that 'P50-4-4-5' will facilitate the development of a new cultivar with low amylose rice.

• 한국식품영양과학회지
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• 제34권3호
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• pp.414-419
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• 2005

실온 이상의 온도(40, 50, 50℃)에서 햅쌀과 저장 쌀을 가온 수침하여 습식제분한 쌀가루의 특성을 조사하였다. 습식처리한 쌀가루는 모두 30% 이상의 수분을 지니고 있었으며, 60℃에서 수침을 한 쌀가루가 40℃, 50℃수침에 비해 수분 함량이 높게 나타났다 쌀가루의 L값은 햅쌀로 제조한 쌀가루에서 약간 높았으며 황색도를 나타내는 b값은 저장 쌀이 햅쌀 백미로 만든 쌀가루보다 높게 나타났다. 햅쌀과 저장 쌀 모두에서 쌀가루의 WAI, WSI 및 gel consistency는 수침 온도가 증가함에 따라 증가하였다 Amylograph peak viscosity, hot paste viscosity, 그리고 cold paste viscosity 모두 수침온도가 증가함에 따라 증가하였으며 특히 저장 쌀 시료에서 보다 큰 폭으로 증가하였다. DSC호화개시온도 및 호화정점온도는 수침온도가 높아짐에 따라 증가하였고, 호화엔탈피는 감소하는 경 향을 보였다. 쌀가루의 입도 분석 결과 저장 쌀이 햅쌀로 제조한 쌀가루에 비해 작은 경향을 보여주었으며 햅쌀의 경우 수침온도가 60℃, 50℃, 40℃순으로 입도분포가 큰 경향을 보여주었다. SEM에 의한 쌀가루의 미세구조 관찰 결과 수침 온도의 증가에 따라 입자 크기의 증가와 손상된 쌀가루 표면이 관찰되었다.

• 한국식품영양과학회지
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• 제34권7호
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• pp.1098-1102
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• 2005

일반 백미를 분무수세한 후 건조하여 제조한 무세미와 부산물로 생성된 무세미 쇄립의 이화학적 특성 및 오염미생물을 분석하였다. 무세미 쌀가루는 명도와 황색도가 원료쌀에 비해 다소 낮았고 수분흡수지 수(WAI)와 수분용해지수(WSI)는 원료쌀에 비해 높게 나타났으며 무세미 쇄립에서 gel consistency가 다소 높게 나타났다. 무세미 쌀가루는 amylo-graph 최고점도와 breakdown이 높았으며 setback은 가장 낮은 반면에 무세미 쇄립은 최고점도와 breakdown이 낮았으며 setback이 높은 것으로 나타났다. 무세미의 DSC 측정결과 호화개시온도와 호화최고온도가 원료쌀에 비해 약간 감소하는 경향이었으며 호화엔탈피는 다소 높게 나타났다. 무세미는 원료쌀에 비해 total bacteria, yeast-like strain의 수가 감소하여 쌀 표면 미생물의 세척 효과에 따른 미생물의 오염정도가 낮았다.

• 한국식품영양학회지
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• 제12권4호
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• pp.409-414
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• 1999

In order to investigate the changes of rice qualities during 4 years storage of paddy stored in ware-house of normal temperature condition. Temperature in warehouse was changed more than 3$0^{\circ}C$ under the influence of average temperature outside of a warehouse. Water content of paddy was not increased over 15% But as average temperature in warehouse was gone up 18$^{\circ}C$ from June to September every years it was supposed that these periods were to be deterioration of rice quality. On investigation of change in paddy it was supposed that these periods were to be deterioration of rice quality. On investigation of change in paddy components during the long term storage, reducing, sugar, amylose, crude protein were increased 0.24%, 19.23%, 7.02% at enterance time to 0.5%, 20.31% 7.46% 4 years later respectively. Max viscosity final visocity and set back value by amylograph were increased 449B,U 610B.U, 161B.U to 493B.U, 715B,U 222B.U but breakdown was decreased 125B.U to 76B.U with the increase of storage period. Gel consistency of rice stored was decreased 44.7mm at enterance time to 39,9mm 4years later. Fatty acid was increased remarkably 4.5KOHmg/100g to 24.4KOHmg/100g. Germination ratio and germ activity of paddy during long-term storage were decreased 97%, 100% to 0%, 0.4% respectively, With the increase of storage period contaminated paddy by molds increased and its by bacteria decreas-ed.

• 대한의용생체공학회:의공학회지
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• 제14권1호
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• pp.51-62
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• 1993

An experimental study was performed for the preparation of living skin-equivalent by the using collagen gel contraction with human fibroblasts as neodermls and cultured human keratinocytes as neoderm is . The results were as follows ; 1) The rate of collagen gel contraction was dependent on the number of fibroblasts into the lattice and collagen contraction was progressed according to the increment of the number of the cells. 2) The rate of collagen gel contraction was progressed according to the decrement of the contraction of the collagen. 3) The rate of gel contraction was progressed according to the increment of serum concentration in the fixed concentration of the fibroblasts and collagen. 4) The lattice contraction was decreased according to the increment of the population doublings of the fibroblasts. 5) Macroscopically, the artificial dermis was gray white in color and tissue-like consistency and elas- ticity. 6) Microscopically, three dimensionally contracted artificial dermis showed more dense fibroblasts and its newly formed collagen fibrils in the matrix than one dimensionally contracted one. 7) Finally prepared skin-equivalent showed good attachment of living stratified keratinocytes to the dermal equivalent microscopically. It has been proposed that newly formed skin-equivalent is suitable for the graft of extensively and deeply burned patients. Shortening of the manufacturing period of skin-equivalent and development of conservation technique as a readily usable state are to be solved for our ongoing works.

• 한국작물학회지
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• 제40권2호
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• pp.236-244
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• 1995

율무의 파종기 및 출종후 일수에 따른 종실의 건물중 및 이화학적 성질변화를 구명하고자 4개 품종을 공시 하여 4월 20일부터 20일 간격으로 5월 30일까지 3회 파종하고 출수후 7일 간격으로 8회 수확하여 시험조사한 결과를 요약하면 다음과 같다. 와 수원6호보다 출수직후부터 출수후 42일까지 종실건물중이 항상 높게 유지되었고, 현입중은 출수직후부터 출수후 28일까지는 비슷한 양상을 나타내었으나 출수후 35일부터는 박피종의 현입중이 더 높았다. 2. 파종기별 종실 및 현입 건물중의 변화는 1차 및 3차 파종구에서는 등숙말기에 등숙속도가 완만해졌으나 2차 파종구는 지속적으로 증가되었고 초기출총한 종실의 백립중은 2주후 출수한 종보 보다 항상 낮았다. 3. 율무현입은 박피종이 후피종보다 등숙속도가 빠르고 파종기가 늦을수록 등숙속도가 빠른 경향이며 초기출수한 종빈보다 중기출수한 종실의 둥숙속도가 빨랐다. 4. 총백질함량은 성숙이 진전됨에 따라 감소하였고 알칼리붕괴도는 성숙이 진전됨 에 따라 높아지는 경향을 보였으며 호거집성은 출수 14일 이후 등숙이 진전되면서 큰 변이를 보이지 않았다. 5. 파종기가 늦어질수록 우백질함량은 약간씩 감소하며 알칼리붕괴도는 높아지는 경향이며 gel의 길이는 큰 변이없이 약간씩 짧아졌다. 6. 파종기가 늦어질수록 율무가루의 아델로그람 특성 중 최고, 최저 및 최종정도와 break down은 감소하였고 set back은 증가하였는데 품종에 따라 변이양상에 차이가 있었다

• Food Science and Biotechnology
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• 제15권3호
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• pp.418-423
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• 2006

Egg white powders (EWPs) were produced after pH adjustment (PH 6-9) of fresh egg white, followed by spray-drying, and foaming and gelling properties of EWPs were examined. EWP produced after pH adjustment to 6.5 (EWP-6.5) resulted in significantly higher foaming ability and gel hardness than control and other pH-adjusted EWP. Significant increases in surface -SH content and surface hydrophobicity of EWP-6.5 coincided with improved foaming ability and gel hardness. Significantly higher consistency index for reconstituted EWP-6.5 indicates unfolding of egg white protein was substantially increased in EWP-6.5. Decreased a-helix content in EWP-6.5 was confirmed by circular dichroism spectral analysis. These results indicate pH adjustment prior to spray-drying leads to structural changes in egg white proteins, significantly affecting major functionalities of EWP.

• 한국식품영양학회:학술대회논문집
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• 한국식품영양학회 2001년도 동계 학술심포지움
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• pp.39-74
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• 2001

The endeavors enhancing the grain quality of high-yielding japonica rice were steadily continued during 1980s∼1990s along with the self-sufficiency of rice production and the increasing demands of high-quality rices. During this time, considerably great, progress and success was obtained in development of high-quality japonica cultivars and qualify evaluation techniques including the elucidation of interrelationship between the physicochemical properties of rice grain and the physical or palatability components of cooked rice. In 1990s, some high-quality japonica rice caltivars and special rices adaptable for food processing such as large kernel, chalky endosperm aromatic and colored rices were developed and its objective preference and utility was also examined by a palatability meter, rapid-visco analyzer and texture analyzer. The water uptake rate and the maximum water absorption ratio showed significantly negative correlations with the K/Mg ratio and alkali digestion value(ADV) of milled rice. The rice materials showing the higher amount of hot water absorption exhibited the larger volume expansion of cooked rice. The harder rices with lower moisture content revealed the higher rate of water uptake at twenty minutes after soaking and the higher ratio of maximum water uptake under the room temperature condition. These water uptake characteristics were not associated with the protein and amylose contents of milled rice and the palatability of cooked rice. The water/rice ratio (in w/w basis) for optimum cooking was averaged to 1.52 in dry milled rices (12% wet basis) with varietal range from 1.45 to 1.61 and the expansion ratio of milled rice after proper boiling was average to 2.63(in v/v basis). The major physicochemical components of rice grain associated with the palatability of cooked rice were examined using japonica rice materials showing narrow varietal variation in grain size and shape, alkali digestibility, gel consistency, amylose and protein contents, but considerable difference in appearance and torture of cooked rice. The glossiness or gross palatability score of cooked rice were closely associated with the peak. hot paste and consistency viscosities of viscogram with year difference. The high-quality rice variety “Ilpumbyeo” showed less portion of amylose on the outer layer of milled rice grain and less and slower change in iodine blue value of extracted paste during twenty minutes of boiling. This highly palatable rice also exhibited very fine net structure in outer layer and fine-spongy and well-swollen shape of gelatinized starch granules in inner layer and core of cooked rice kernel compared with the poor palatable rice through image of scanning electronic mcroscope. Gross sensory score of cooked rice could be estimated by multiple linear regression formula, deduced from relationship between rice quality components mentioned above and eating quality of cooked rice, with high Probability of determination. The ${\alpha}$ -amylose-iodine method was adopted for checking the varietal difference in retrogradation of cooked rice. The rice cultivars revealing the relatively slow retrogradation in aged cooked rice were Ilpumbyeo, Chucheongbyeo, Sasanishiki, Jinbubyeo and Koshihikari. A Tongil-type rice, Taebaegbyeo, and a japonica cultivar, Seomjinbyeo, shelved the relatively fast deterioration of cooked rice. Generally, the better rice cultivars in eating quality of cooked rice showed less retrogiadation and much sponginess in cooled cooked rice. Also, the rice varieties exhibiting less retrogradation in cooled cooked rice revealed higher hot viscosity and lower cool viscosity of rice flour in amylogram. The sponginess of cooled cooked rice was closely associated with magnesium content and volume expansion of cooked rice. The hardness-changed ratio of cooked rice by cooling was negatively correlated with solids amount extracted during boiling and volume expansion of cooked rice. The major physicochemical properties of rice grain closely related to the palatability of cooked rice may be directly or indirectly associated with the retrogradation characteristics of cooked rice. The softer gel consistency and lower amylose content in milled rice revealed the higher ratio of popped rice and larger bulk density of popping. The stronger hardness of rice grain showed relatively higher ratio of popping and the more chalky or less translucent rice exhibited the lower ratio of intact popped brown rice. The potassium and magnesium contents of milled rice were negatively associated with gross score of noodle making mixed with wheat flour in half and the better rice for noodle making revealed relatively less amount of solid extraction during boiling. The more volume expansion of batters for making brown rice bread resulted the better loaf formation and more springiness in rice bread. The higher protein rices produced relatively the more moist white rice bread. The springiness of rice bread was also significantly correlated with high amylose content and hard gel consistency. The completely chalky and large gram rices showed better suitability for fermentation and brewing. Our breeding efforts on rice quality improvement for the future should focus on enhancement of palatability of cooked rice and marketing qualify as well as the diversification in morphological and physicochemical characteristics of rice grain for various value-added rice food processings.