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http://dx.doi.org/10.4333/KPS.2009.39.5.381

Comparison of Oral versus Rectal Administration of Processed-Scutellaria baicalensis on Colonic Inflammation in Mice  

Choi, Yeon-Ah (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University and Wonkwang-Oriental Medicines Research Institute)
Kim, Dae-Ki (Department of Immunology, Chonbuk National University Medical School)
Chun, Myung-Kwan (College of Pharmacy, Chosun University)
Choi, Hoo-Kyun (College of Pharmacy, Chosun University)
Lee, Young-Mi (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University and Wonkwang-Oriental Medicines Research Institute)
Publication Information
Journal of Pharmaceutical Investigation / v.39, no.5, 2009 , pp. 381-386 More about this Journal
Abstract
We previously showed that the water extracts of rice wine-baked Scutellaria baicalensis Georgi (RWBS) ameliorated colonic inflammation more than crude Scutellaria baicalensis (CS) after oral administration. The aim of this study is to compare the effect of rectal and oral administration of RWBS in the experimental colitis. Experimental colitis was induced in mice by daily treatment with 5% dextran sulfate sodium (DSS) in the drinking water for 7 days. Water was used as vehicle of oral administration, while Carbopol/PEG mucoadhesive gel was used as vehicle of rectal administration. RWBS and RWBS gel (RWBSG) were administered once per day for 7 days. RWBS and RWBSG significantly attenuated the disease activity index (DAI) calculated as the sum of scores of body weight loss, stool consistency and rectal bleeding. Furthermore, RWBS and RWBSG reduced the mucosal myeloperoxidase activity and COX-2 (cyclooxygenase-2) expression in colon tissue. Anti-inflammatory effect of CS on colonic inflammation was increased by baking with rice wine in both oral and rectal administration. Moreover, anti-inflammatory effects of oral administration on colonic inflammation was greater than those of rectal administration. Further study would be required for the development of intra-rectal formulation.
Keywords
Scutellaria baicalensis; Colitis; Mucoadhesive gel; Inflammation;
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1 K. Ono and H. Nakane, Mechanisms of inhibition of various cellular DNA and RNA polymerases by several flavonoids, J. Biochem., 108, 609-613 (1990).   DOI
2 B.H. Lee, S.J. Lee, T.H. Kang, D.H. Kim, D.H. Sohn, G. Ko and Y.C. Kim, Baicalein, an in vitro antigenotoxic compound from Scutellaria baicalensis, Planta Med., 66, 70-71 (2000).
3 T. Hong, G.B. Jin and J.C. Cyong, Effect of components of Oren-gedoku-to (Huang-Lian-Jie-Du-Tang) on murine colitis induced by dextran sulfate sodium, J. Trad. Med., 17, 173-179 (2000).
4 B.Y. Kang, S.W. Chung, S.H. Kim, D. Cho, T.S. Kim. Involvement of nuclear factor-kappaB in the inhibition of interleukin-12 production from mouse macrophages by baicalein, a flavonoid in Scutellaria baicalensis, Planta Med., 69, 687-691 (2003).   DOI   ScienceOn
5 Y.C. Shen, W.F. Chiou., Y.C. Chou, C.F. Chen, Mechanisms in mediating the anti-inflammatory effects of baicalin and baicalein in human leukocytes, Eur. J. Pharmacol., 465, 171-181 (2003).   DOI   ScienceOn
6 O.H. Kang, D.K. Kim, Y.A. Choi, H.J. Park, J. Tae, C.S. Kang, S.C. Choi, Y.H. Nah, H.K. Lee and Y.M. Lee, Suppressive effect of non-anaphylactogenic anti-IgE antibody on the development of dextran sulfate sodium-induced colitis, Int. J. Mol. Med., 18, 893-899 (2006).
7 M. Campieri, New steroids and new salicylates in inflammatory bowel disease: a critical appraisal, Gut, 50, III 43-46 (2002).   DOI
8 Z.H. Shao, T.L. Vanden Hoek, Y. Qin, L.B. Becker, P.T. Schumacker, C.Q. Li, L. Dey, E. Barth, H. Halpern, G. M.Rosen and C.S. Yuan, Baicalein attenuates oxidant stress in cardiomyocytes, Am. J. Physiol., 282, H999-H1006 (2002).
9 C.J. Chen, S.L. Raung, S.L. Liao, and S.Y. Chen, Inhibition of inducible nitric oxide synthase expression by baicalein in endotoxin/cytokine-stimulated microglia, Biochem. Pharmacol., 5, 957-965 (2004).   DOI   ScienceOn
10 M. Kimata, M. Shichijo, T. Miura, I. Serizawa, N. Inagaki and H. Nagai, Effects of luteolin, quercetin and baicalein on immunoglobulin E-mediated mediator release from human cultured mast cells, Clin. Exp. Allergy, 30, 501-508 (2000).   DOI   ScienceOn
11 Y.A. Choi, O.H. Kang, H.J. Park, J. Tae, D.K. Kim, C.S. Kang, S.C. Choi, K.J. Yun, S.J. Choi, Y.H. Nah, Y.H. Kim, K.H. Bae and Y.M. Lee, Effect of processed-Scutellaria baicalensis on dextran sulfate sodium-induced colitis in mice, Int .J. Mol. Med., 16, 667-672 (2005).
12 C.C. Lin and D.E. Shieh, The anti-inflammatory activity of Scutellaria rivularis extracts and its active components, baicalin, baicalein and wogonin, Am. J.Chin. Med., 24, 31-36 (1996).   DOI   ScienceOn
13 C.M. Lehr, J.A. Bouwstra, W. Kok, A.G. De Boer, J.J. Tukker, J.C. Verhoef, D.D. Breimer and H.E. Junginger, Effects of the mucoadhesive polymer polycarbophil on the intestinal absorption of a peptide drug in the rat, J. Pharm. Pharmacol., 44, 402-407(1992).   DOI
14 G. Bonacucina, S. Martelli and G.F. Palmieri. Rheological, Mucoadhesive and release properties of Carbopol gels in hydrophilic cosolvents, Int. J. Pharm., 282, 115-130 (2004).   DOI   ScienceOn
15 S.N.S. Murthy, H.S. Cooper, H. Shim, R.S. Shah, S.A. Ibarahim and D.J. Sedergran, Treatment of dextran sulfate sodiuminduced murine colitis by intracolonic cyclosporine, Dig. Dis. Sci., 38, 1722-1734 (1993).   DOI
16 M.Y. Lai, S.L. Hsiu, S.Y. Tsai, Y.C. Hou and P.D. Chao. Comparison of metabolic pharmacokinetics of baicalin and baicalein in rats, J. Pharm. Pharmacol., 55, 205-209 (2003).   DOI   ScienceOn
17 C. Fiocchi, Inflammatory bowel disease: etiology and pathogenesis, Gastroenterology, 115, 182-205 (1998).   DOI   ScienceOn
18 F. Rizzello, P. Gionchetti, A. Venturi and M. Campieri, Review article: medical treatment of severe ulcerative colitis, Aliment. Pharmacol. Ther., 17, 7-10 (2003)   DOI   ScienceOn
19 T. Hong, G.B. Jin, S. Cho and J.C. Cyong, Evaluation of the anti-inflammatory effect of baicalein on dextran sulfate sodium induced colitis in mice, Planta Med., 68, 268-271 (2002).   DOI   ScienceOn
20 N.M. Davies, S.J. Farr, J. Hadgraft and I.W. Kellaway, Evaluation of mucoadhesive polymers in ocular drug delivery. I. Viscous solutions, Pharm. Res., 8, 1039-1043 (1991).   DOI
21 Z. Yang, B. Zhang, L. Cai, Study on processing conditions of wine baked radix Achyranthis bidentatae, Zhong Yao Cai., 23, 681-682 (2000).
22 B.Q. Li, T. Fu, W.H. Gong, N. Dunlop, H.F. Kung, Y. Yan, J. Kang and J.M. Wang, The flavonoid baicalin exhibits antiinflammatory activity by binding to chemokines, Immunopharmacology, 49, 295-306 (2000).   DOI   ScienceOn