• Title/Summary/Keyword: Gaussian mixture method

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Segmentation Method of Overlapped nuclei in FISH Image (FISH 세포영상에서의 군집세포 분할 기법)

  • Jeong, Mi-Ra;Ko, Byoung-Chul;Nam, Jae-Yeal
    • The KIPS Transactions:PartB
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    • v.16B no.2
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    • pp.131-140
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    • 2009
  • This paper presents a new algorithm to the segmentation of the FISH images. First, for segmentation of the cell nuclei from background, a threshold is estimated by using the gaussian mixture model and maximizing the likelihood function of gray value of cell images. After nuclei segmentation, overlapped nuclei and isolated nuclei need to be classified for exact nuclei analysis. For nuclei classification, this paper extracted the morphological features of the nuclei such as compactness, smoothness and moments from training data. Three probability density functions are generated from these features and they are applied to the proposed Bayesian networks as evidences. After nuclei classification, segmenting of overlapped nuclei into isolated nuclei is necessary. This paper first performs intensity gradient transform and watershed algorithm to segment overlapped nuclei. Then proposed stepwise merging strategy is applied to merge several fragments in major nucleus. The experimental results using FISH images show that our system can indeed improve segmentation performance compared to previous researches, since we performed nuclei classification before separating overlapped nuclei.

Clustering of sediment characteristics in South Korean rivers and its expanded application strategy to H-ADCP based suspended sediment concentration monitoring technique (한국 하천의 지역별 유사특성의 군집화와 H-ADCP 기반 부유사 농도 관측 기법에의 활용 방안)

  • Noh, Hyoseob;Son, GeunSoo;Kim, Dongsu;Park, Yong Sung
    • Journal of Korea Water Resources Association
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    • v.55 no.1
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    • pp.43-57
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    • 2022
  • Advances in measurement techniques have reduced measurement costs and enhanced safety resulting in less uncertainty. For example, an acoustic doppler current profiler (ADCP) based suspended sediment concentration (SSC) measurement technique is being accepted as an alternative to the conventional data collection method. In Korean rivers, horizontal ADCPs (H-ADCPs) are mounted on the automatic discharge monitoring stations, where SSC can be measured using the backscatter of ADCPs. However, automatic discharge monitoring stations and sediment monitoring stations do not always coincide which hinders the application of the new techniques that are not feasible to some stations. This work presents and analyzes H-ADCP-SSC models for 9 discharge monitoring stations in Korean rivers. In application of the Gaussian mixture model (GMM) to sediment-related variables (catchment area, particle size distributions of suspended sediment and bed material, water discharge-sediment discharge curves) from 44 sediment monitoring stations, it is revealed that those characteristics can distinguish sediment monitoring stations regionally. Linking the two results, we propose a protocol determining the H-ADCP-SSC model where no H-ADCP-SSC model is available.