• Journal of Oriental Neuropsychiatry
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• v.3 no.1
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• pp.62-65
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• 1992

120 health korean and 43 korean with cancer (gastric, rectal, breast, aged 20 to C9 years in Yanbian district) were measured by atomic absorbtion spectrometer(Type WFX-II). The results obtained are as follows; in heath1 person hair, Zn $103.87{\pm}13.9$ppm, Cu $10.77{\pm}2.62$, Cr$0.288{\pm}0.121$ppm, Zn/Cu $10.77{\pm}3.24$. There is no significant difference betwen male and female, and korean and Han. Zn contents in hairs of patients with cancer significantly lower than that of health persons ; Cu and Cr contents are nearly same in both groups.

• Journal of Pharmaceutical Investigation
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• v.33 no.4
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• pp.267-272
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• 2003

Herpes simplex virus (HSV) thymidine kinase (TK) has been widely used for suicidal gene therapy in combination with nucleoside analogs such as ganciclovir (GCV). The use of HSV-TK is limited due to the side effect of GCV at high concentrations. Previous studies showed that stable transfectants of mutant HSV-TK with enhanced affinity to GCV were killed at lower GCV concentrations. In this study, we tested whether mutant HSV-TK can provide enhanced suicidal effect when transiently transfected with Epstein-Barr virus (EBV)-based plasmid. EBV-based plasmid which contains OriP and EBNA-1 sequence is well known for a stable episomal maintenance in human cells. Optimal transfection condition was assessed for SNU-638 gastric cancer cell line using polyetylnimine (PEI). Maximum transfection efficiency was achieved when DNA:PEI was 1:3 (w/v). Cytotoxicities of mutant and wild type HSV-TK were compared before and after partially selecting transfected cells. The cells were sensitive to $100\;{\mu}g/ml$ hygromycin. Following GCV treatment, more cells were killed after hygromycin selection than before selection. The mutant HSV-TK showed enhanced cytotoxicity compared with the wild type HSV-TK. Our results suggest that the EBV-based plasmid encoding mutant HSV-TK may be useful to treat the diseases caused by uncontrolled cell proliferation such as cancer and rheumatoid arthritis.

• Journal of Microbiology and Biotechnology
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• v.19 no.12
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• pp.1557-1564
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• 2009

Human CDX2 is known as a caudal-related homeodomain transcription factor that is expressed in the intestinal epithelium and is important in differentiation and maintenance of the intestinal epithelial cells. The caudal-related homeobox proteins bind DNA according to a helix-turn-helix structure, thereby increasing the structural stability of DNA. A cancer-tumor suppressor role for Cdx2 has been shown by a decrease in the level of the expression of Cdx2 in colorectal cancer, but the mechanism of transcriptional regulation has not been examined at the molecular level. We developed a large-scale system for expression of the recombinant, novel CDX2, in Escherichia coli. A highly purified and soluble CDX2 protein was obtained in E. coli strain BL21(DE3)RIL and a hexahistidine fusion system using Ni-NTA affinity column, anion exchange, and gel filtration chromatographies. The identity and secondary structure of the purified CDX2 protein were confirmed by MALDI-TOF MS, Western blot, and a circular dichroism analyses. In addition, we studied the DNA-binding activity of recombinant CDX2 by ELISA experiment and isolated human CDX2-binding proteins derived from rat cells by an immobilized GST-fusion method. Three CDX2-binding proteins were found in the gastric tissue, and those proteins were identified to the homeobox protein Hox-D8, LIM homeobox protein 6, and SMC1L1 protein.

• Journal of Applied Biological Chemistry
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• v.57 no.1
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• pp.41-45
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• 2014

In the present study, red ginseng extracts were fermented by Paecilomyces tenuipes and the protopanaxdiol-type ginsenosides in the extracts were bio-transformed to F2, Rg3, Rg5, Rk1, Rh2, and CK determined by a high-pressure liquid chromatography analysis. It indicates that P. tenuipes is a microorganism to biotransform protopanaxdiol-type ginsenosides to their less glucosidic metabolites. Other biotransformed metabolites during fermentation were also analyzed using a GC-MS and identified as 2-methyl-benzaldehyde, 4-vinyl-2-methylphenol, palmitic acid, and linoleic acid. Antiulcerogenic activity of the fermented red ginseng extract (FRGE) on gastric mucosal damage induced by 0.15 M HCl in ethanol in rats was evaluated. FRGE was shown to have a potent protective effect on gastritis with 60.5% of inhibition rate at the dose of 40 mg/kg when compared to 54.5% of the inhibition rate at the same dose for stillen, the currently used medicine for treating gastritis. Linoleic acid showed a strong inhibition on gastritis with 79.3% of inhibition rate at the dose of 40.0 mg/kg. FRGE exhibited a distinct anticancer activity including growth inhibition of the two human colon cancer cells HT29 and HCT116. HT29 cells were less susceptible to FRGE in comparison with HCT116 cells. Taken together, fungal fermentation of the red ginseng extract induced hydrolysis of some ginsenosides and FRGE exhibited potent antiulcerogenic and anticancer activities. These results refer to use FRGE as a new source for treating human diseases.

• The Journal of the Korean life insurance medical association
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• v.4 no.1
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• pp.24-37
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• 1987

During the period from september, 1985 to september, 1986, 1,005 cases(475cases in male, 529 cases in female) of employees and family member were observed for the general physical examination(Human-dock) in Medical Department of Daehan Life Insurance Co. Ltd. The results were as follows. 1) The occurrence of obesity cases were observed as 130 cases(12.9%), and among the 130 cases, 34 cases(26.2%) were male and 96 cases(73.8%) were female. 2) Diabetes mellitus patients were detected as 93 cases(9.3%), and 53 cases(57.1%) were male and 40 cases(43.0%) were female. The most frequent age groups were demonstrated in age of 5th and 6th decades. 3) Hypertension patients were 85 cases(8.5%), and among the 85 cases, 42 cases (49.4%) were male and 43 cases(50.6%) were female. The most frequent age groups were 5th and 6th decades, and complication of hypertensive retinopathy revealed 54 cases(63.5%). 4) Hyperlipidemia cases were observed as 71(7.1%), and 42 cases(59.2%) were male and 29 cases(40.8%) were female. The most frequent age groups were 5th and 6th decades. 5) 69 cases(6.9%) of positive reaction of HBs Ag and 46 cases(4.6%) of abnormal erectrocardiography were detected in the total examination cases. 6) Abnormalities of liver function were observed as 58 cases(5.8%), and 46 cases(79.3%) were male and 12 cases(20.7%) were female. In ultrasonographic study, 25 cases of fatty liver were obtained in the abnormality cases of liver function. 7) Cholelithiasis and gastroduodenal ulcer patients were detected as 2 cases(2.0%) respectively. 8) In the total examination cases, pulmonary tuberculosis, positive reaction of syphilis and renal cysts(ultrasonography) were obtained as 9 cases(0.9%), 7 cases(0.7%) and 4 cases(0.4%) respectively. 9) In the total examination cases, gastric cancer and liver cancer patients were detected as 2 cases(0.2%) respectively.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.7
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• pp.2999-3001
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• 2015

Background: This study aimed to evaluate upper gastrointestinal polyps detected during esophago-gastroduodenoscopy tests. Materials and Methods: We conducted a retrospective analysis on data regarding 55,987 upper gastrointestinal endoscopy tests performed at the endoscopy unit of Istanbul Education and Research Hospital between January 2006 and June 2012. Results: A total of 66 upper gastrointestinal polyps from 59 patients were analyzed. The most common clinical symptom was dyspepsia, observed in 41 cases (69.5%). The localizations of the polyps were as follows: 29 in the antrum (43.9%), 15 in the corpus (22.7%), 11 in the cardia (16.7%), 3 in the fundus (4.54%), 3 in the second portion of the duodenum (4.54%), 2 in the bulbus (3.03%) and 3 in the lower end of the esophagus (4.54%). Histopathological types of polyps included hyperplastic polyps (44) (66.7%), faveolar hyperplasia (8) (12.1%), fundic gland polyps (4) (6.06%), squamous cell polyps (4) (6.06%), hamartomatous polyps (3) (4.54%), and pyloric gland adenoma (3) (4.54%). Histopathological analysis of the gastric mucosa showed chronic atrophic gastritis in 30 cases (50.84%), HP infection in 33 cases (55.9%) and intestinal metaplasia in 19 cases (32.20%). In 3 cases with multiple polyps, adenocarcinoma was detected in hyperplastic polyps. Conclusions: Among polypoid lesions of the upper gastrointestinal tract, the most common histological type is hyperplastic polyps. Generally, HP infection is associated with chronic atrophic gastritis and intestinal metaplasia. The incidence of adenocarcinoma tends to be higher in patients with multiple hyperplastic polyps.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.1
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• pp.53-56
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• 2013

Background: It is known that blood group antigens are related to the development of peptic ulcer and gastric carcinoma. Infections due to H. pylori are most widespread among the developing regions due to poor standard of public health. This study sought to determine the association of H. pylori with ABO blood groups, age, gender, and smoking status among inpatients at a public sector hospital in Karachi. Materials and Methods: A cross-sectional study was conducted at endoscopy suite at a public sector hospital in Karachi in the year 2011. All the symptomatic patients coming for upper GIT endoscopy were included in this study. Results: Biopsy for histopathology was taken from 93 patients, with an age range from 15-65 years. Age group of 15 to 20 years was found to be associated with H. pylori infection but without significance (p-value 0.83). In all, 36 (38.7%) turned out to be H. pylori positive with a significant male preponderance (p=0.04). Distribution of ABO blood groups in H. pylori positive group were A=31.4%, B=15.4%, AB=25.0% and O=53.7%, with a statistically significant link for blood group O (p=0.05). Rhesus factor was also compared but significant relationship was evident (p-value 0.73). Conclusions: This study demonstrated that H. pylori infection can be related to ABO blood group, middle age persons and male gender. People of blood group O are more prone to develop infection related gastritis, ulcers, and even perforations, so they should be more cautious against transmission of the bacterium.

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.7
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• pp.1157-1161
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• 2013

The purpose of this work was to measure the changes in ${\gamma}$-aminobutyric acid (GABA) and polyphenolic content of two different rice varieties (Geunnun and Ilpum), as well as the antiproliferative activities of both germinated brown rice varieties in cancer cells. The contents of GABA in Geunnun and Ilpum, especially in the bran of Ilpum increased significantly after germination. The content of polyphenol in Geunnun also increased after germination, but the contents of flavonoid in both varieties decreased after germination. A significant increase in the antiproliferative activity of both varieties on human lung and gastric cancer cell line was observed after germination.

• The Korean Journal of Food And Nutrition
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• v.18 no.2
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• pp.115-126
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• 2005

This Quantitative study was investigated to examine the relationship between self-esteem on body image and disordered eating patterns in some university students. This study used a cross-sectional study design. Total 347 students participated in this study (88 male and 259 female) among three universities. The assessment of eating disorder was conducted by Eating Attitudes Test (EAT-26), a score of >or=20 identified individuals likely have an eating disorder, including anorexia nervosa and bulimia nervosa. Ideal body weight and current body weight were completed with self-evaluation. A distorted body image was independently related to the eating problem in the Logistic regression analysis. There were stronger relationships between their EAT-26 scores and their expected weight changes and weight control behaviors. Compared with the men, women showed highly wishful to loss for current body weight. In the relationship between score of EAT-26 and BMI distance by sex, levels of eating disorder showed linearly elevated toward increased BMI distance (Ideal BMI-Current BMI) (F-value=18.794, p<0.0001) in women, but there were not significant in men (F-value=2.028, p>0.05). In estimate on state-trait anxiety inventory according to quartile of BMI distance by sex, levels of state-trait anxiety were not significant difference by degree of body dissatisfaction in all sex. In addition, higher distorted body image was significantly increased numbers of weight control method and experience of side effects in female, but there were not showed significant relation between two variables in male.

• The Korean Journal of Food And Nutrition
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• v.19 no.2
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• pp.111-125
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• 2006

This quantitative study was conducted to examine the relationship between weight control behaviors and disordered eating patterns in some university students. This study used a cross-sectional study design. A total of 347 students from three universities participated in this study (88 male and 259 female) Eating disorders were assessed using the Eating Attitudes Test (EAT-26); a score of =20 identifies individuals who likely have an eating disorder, including anorexia nervosa and bulimia nervosa. A score for healthy dietary behaviors was obtained by self-assessment on a healthy diet scale (20-item questionnaire), and the severity of any state-trait anxiety was calculated by the state-trait anxiety inventory (40-item questionnaire). In the analyzed results, the percentage of participants with experience of weight control was 58% in male and 73% in female. The subjects with a high risk of an eating disorder (score of =20 of EAT-26) were 44.3% ($mean{\pm}S.D;\;18.9{\pm}13.4$) of the males, and 57.9% ($mean{\pm}S.D;\;23.2{\pm}11.6$) of the females. Higher Body Mass Index (BMI) was significantly related with an increased risk for an eating disorder in females, but not in males. In the group who had attempted weight control of all types, there was a severe risk of an eating disorder. Increased eating disorder risk was significantly related with weight control behaviors such as a higher number of attempts at weight control, having used medication, having experienced side effects, and having experienced disease for both sexes. Therefore, the results of this paper showed that detrimental behaviors of weight control are connected to an increased risk of eating disorders. Consequently, education regarding the correct, behaviors of weight control is necessary to prevent eating disorders in adolescents.