• Journal of Microbiology and Biotechnology
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• 제19권7호
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• pp.713-717
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• 2009

The dependence of the catalytic properties of horseradish peroxidase on the structural changes of ionic liquids was investigated with two water-miscible ionic liquids, N-butyl-3methypyridinium tetraftuoroborate ([$BMP_y$][$BF_4$]) and 1-butyl-3-methylimidazolium methylsulfate ([BMIM][$MeSO_4$]), each of which shares an anion ($BF_4^-$) or a cation ($BMIM^+$) with 1-butyl-3-methylimidazolium tetraftuoroborate ([BMIM][$BF_4$]), respectively. The oxidation of guaiacol (2-methoxyphenol) with $H_2O_2$was used as a model reaction. In order to minimize the effect of solution viscosity on the kinetic constants of the enzymatic catalysis, the enzymatic reactions for the kinetic study were performed in water-ionic liquid mixtures containing 25% (v/v) ionic liquid at maximum. Similarly to the previously reported results for [BMIM][$BF_4$], as the concentration of [$BMP_y$][$BF_4$] increased, the $K_m$value increased with a decrease in the $k_{cat}$value: the $K_m$value increased markedly from 2.8 mM in 100% water to 12.6 mM in 25% (v/v) ionic liquid, indicating that ionic liquid significantly weakens the binding affinity of guaiacol to the enzyme. On the contrary, [BMIM][$MeSO_4$] decreased the Km value to 1.4 mM in 25% (v/v) ionic liquid. [BMIM][$MeSO_4$] also decreased $k_{cat}$more than 3-folds [from 13.8 $s^{-1}$in 100% water to 4.1 $s^{-1}$in 25% (v/v) ionic liquid]. These results indicate that the ionic liquids interact with the enzyme at the molecular level as well as at a macroscopic thermodynamic scale. Specifically, the anionic component of the ionic liquids influenced the catalysis of horseradish peroxidase in different ways.

• 정보처리학회논문지D
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• 제10D권1호
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• pp.161-166
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• 2003

제품을 생산하고 공급하는데 소요되는 주문리드타임과 달리 주문이 처리되는데 소요되는데 지연되는 것을 정보리드타임이라고 하는데, 이는 공급체인에서 개별기업들이 수요예측의 변동폭을 높이게 되며 이것이 바로 비용 상승요인으로 작용하게 된다. 본 논문에서는 직렬형 가치사슬에서 정보리드타임이 공급체인 시스템에 주는 영향을 알아본다. 특별히, MIT 시뮬레이션 모형을 동해서 실험을 수행하며, 아래와 같은 두 가지의 이슈를 다룬다. 첫째는 비용-이익 관점에서 물류 리드타임보다 정보리드타임의 개선이 효과적인가\ulcorner 둘째는 정보리드타임이 고객에게 보다 인접한 기업(downstream)이 고객하고 떨어져 있는 기업(upstream)보다 비용을 크게 상승시키고 있는지에 대한 물음이다. 사례 분석에서는 공급체인상의 개변기업들이 갖는 정보리드타임의 중요성을 지적하고, 개별기업간의 정보리드타임의 차이 분석을 통해서 고객의 수요와 요구를 직접 받는 기업에서 정보리드타임의 중요성이 큼을 인증하고 있다.

• 대한환경공학회지
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• 제28권5호
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• pp.573-576
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• 2006

The efficacy of integrated ozone oxidation-biodegradation treatment was examined in the treatment of petrochemical wastewater with a special focus on the overall treatment time. When raw wastewater with chemical oxygen demand(COD) of 70-80 mg/L was oxidized by ozone, approximately 20% of initial COD was removed in less than 1.5 min at a dosing rate of 400 mg $O_3/L{\cdot}h $. No further decrease in COD was observed for the extended ozone treatment up to 30 min. Biological treatment alone showed a rapid reduction of COD to 40-50 mg/L, subsequently resulting in the decreased rate of COD removal. Pre-treatment by ozone before biological treatment did not significantly affect the specific rate of COD removal in a biological treatment. When ozone oxidation followed biological treatment, the extent of COD removal by ozone oxidation was greater compared to that of biologically-treated wastewater for a shorter time. Taken together, it was decided that the biological treatment time could be reduced if the treatment processes of concern will be properly arranged.

• Genomics & Informatics
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• 제19권4호
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• pp.39.1-39.8
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• 2021

Tamoxifen (TAM) is an anticancer drug used to treat estrogen receptor (ER)-positive breast cancer. However, its ER-independent cytotoxic and antifungal activities have prompted debates on its mechanism of action. To achieve a better understanding of the ER-independent antifungal action mechanisms of TAM, we systematically identified TAM-sensitive genes through microarray screening of the heterozygous gene deletion library in fission yeast (Schizosaccharomyces pombe). Secondary confirmation was followed by a spotting assay, finally yielding 13 TAM-sensitive genes under the drug-induced haploinsufficient condition. For these 13 TAM-sensitive genes, we conducted a comparative analysis of their Gene Ontology (GO) 'biological process' terms identified from other genome-wide screenings of the budding yeast deletion library and the MCF7 breast cancer cell line. Several TAM-sensitive genes overlapped between the yeast strains and MCF7 in GO terms including 'cell cycle' (cdc2, rik1, pas1, and leo1), 'signaling' (sck2, oga1, and cki3), and 'vesicle-mediated transport' (SPCC126.08c, vps54, sec72, and tvp15), suggesting their roles in the ER-independent cytotoxic effects of TAM. We recently reported that the cki3 gene with the 'signaling' GO term was related to the ER-independent antifungal action mechanisms of TAM in yeast. In this study, we report that haploinsufficiency of the essential vps54 gene, which encodes the GARP complex subunit, significantly aggravated TAM sensitivity and led to an enlarged vesicle structure in comparison with the SP286 control strain. These results strongly suggest that the vesicle-mediated transport process might be another action mechanism of the ER-independent antifungal or cytotoxic effects of TAM.

• 한국양식학회지
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• 제9권3호
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• pp.223-232
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• 1996

산지가 다른 3지역의 바지락, Ruditapes philippinarum 종패를 재료로 전남 승주군 해룡면 신성포의 바지락 양식장에서 산지별 및 방양밀도별로 성장과 생존에 관하여 조사하였다. 실험기간 동안의 수온은 4.5 (1월)${\~}26.0^{\circ}C$ (8월) 범위로 나타났으며, 비중은 1.0216 (8월)${\~}1.0248$ (2월) 범위였다. 산지별 바지락의 양성결과, 각장 성장은 하동산이 $2.30{\pm}0.05$ cm에서 $3.29{\pm}0.02$ cm, 울산산이 $1.32{\pm}0.02$ cm에서 $3.15{\pm}0.02$ cm, 고창산이 $1.24{\pm}0.03$ cm에서 $3.18{\pm}0.02$ cm로 성장하여, 고창산의 성장이 하동산과 울산산에 비해 빠르게 나타났다. 밀도별 양성실험에서 개시시 각장 $1.32{\pm}0.02$ cm 내외였던 것이 실험 종료시 $m^2$당 1,000개체 실험구가 $3.21{\pm}0.02$ cm로 2,000개체 실험구 $2.61{\pm}0.02$ cm 보다 성장이 빨랐으며, 전중도 1,000, 1,500 및 2,000 개체구에서 각각 $5.47{\pm}0.22\;g,\;5.12{\pm}0.788\;g,\;4.62{\pm}1.62\;g$로 나타나 밀도가 낮은 실험구에서 성장이 좋았다. 산지별 바지락의 생존율은 실험 종료시 하동산, 울산산, 고창산이 각각 $69.4\%,\;63.8\%,\;41.7\%$로 고창산이 가장 낮았으며, 밀도별 생존율은 1,000개체, 1,500개체, 2,000개체 실험구가 각각 $70.8\%,\;67.8\%,\;59.0\%$로 밀도가 높을 수록 낮아지는 경향을 보였다.