• 치위생과학회지
• /
• 제10권2호
• /
• pp.79-83
• /
• 2010

소염진통제인 Ketorolac tromethamine에 치자엑스 복합제제를 혼합하여 4종의 하이드로겔을 제조한 다음 주성분의 확인, 점도, 표면장력, 인장강도 및 생체부착성 실험을 통해 다음과 같은 결론을 얻었다. 1. KGE 및 KGH gel 제제 중 Ketorolac tromethamine의 함량은 1.02~0.97%이었고, Geniposide의 함량은 KGE gel A와 C에서 0.34%이었으나, KGH gel B와 D에서 0.11%로 낮아졌고, Genipin의 함량은 KGE gel A와 C에서는 나타나지 않았으나, KGH gel B와 D에서 0.13%로 나타났다. 2. Gel 제제의 온도에 따른 점도는 gel 화제로 Carbopol 940을 단독으로 사용한 gel이 Poloxamer 407을 추가한 gel보다 더 높은 점도를 유지하였으며, 각 제제에서의 표면장력은 $37^{\circ}C$에서 34.77~40.58 dyne/cm를 나타냈다. 제제의 인장강도는 KGH gel B에서 대조군에 비하여 3.5배 정도 높은 인장강도를 나타냈다. 3. 생체부착성은 등피부 상부(표피층)와 배피부의 경우 KGH gel B 에서 50.62 N으로 나타나 대조군에 비해 5배 정도 높은 수치를 나타내었고, 등피부 하부(진피층)와 배피부의 경우 KGH gel B의 에서 35.93 N으로 나타나 대조군에 비해 3.5배 정도 높은 수치를 나타냈다.

• 치위생과학회지
• /
• 제5권3호
• /
• pp.113-117
• /
• 2005

소염진통제인 케토롤락트로메타민에 치자엑스 복합제제를 혼합하여 4종의 하이드로겔 제제를 제조한 후 인장강도측정, 피부투과 및 치주낭 감소율측정 실험을 통해 다음과 같은 결론을 얻었다. 1. 제제의 인장강도는 KGH gel B에서 대조군에 비하여 3.5배 정도 높은 인장강도를 나타내었다. 2. 케토롤락트로메타민의 피부투과량은 8시간 동안 KGH gel B에서 $105.62{\mu}m/cm^2$로 가장 높았고, geniposide 투과량은 KGE gel A에서 $73.8{\mu}m/cm^2$ 비교적 높았으나 가수분해물인 genipin의 투과량은 KGH gel B에서$50.17{\mu}m/cm^2$ 가장 높은 투과량을 나타내었다. 3. 치주낭 감소율은 4주 후 KGE gel A는 대조군에 비하여 23.85%의 감소율을 나타내었으나 유의성 있는 차이는 나타내지 못하였으며, KGH gel B는 대조군에 비하여 29.46%의 감소율을 보여 유의성 있는 치료효과를 나타내었다.

• 동의생리병리학회지
• /
• 제22권5호
• /
• pp.1106-1111
• /
• 2008

Sa-Hwang-San, which is invented by Jhun Eul for the treatment of tongue thrust of Spleen heat, is first mentioned on 'So Ah Yak Jeung Jik Gyul'. It is consisted of Gardeniae Fructus, Agastache rugosa, Glycyrrhizae Radix, Gypsum Fibrosum, Ledebouriellae Radix. According to 'Dong Ui Bo Gam', this medicine cures spleen heat, sore mouth and halitosis. In the viewpoint of pharmacology, this medicine as a whole can treat spleen stagnancy, spleen heat and stomach heat. However, there are some differences between the opinions of 'So Ah Yak Jeung Jik Gyul' and 'Dong Ui Bo Gam' the principal medicine of the former is Ledebouriellae Radix, while that of the latter is Gardeniae Fructus. Spleen stagnancy is the malfunction of spleen under the presence of the body fluid. Symptoms are abdominal dropsy and powerlessness. Spleen heat is the insufficiency of body fluid due to the heat of the spleen, while the function of the spleen is normal. Symptoms are sore mouth, halitosis and maxillary stagnancy. In clinical cases, Sa-Hwang-San has been found effective to the individuals with large, lowered, soft, and slanted mouths and lips. It was also beneficial for the YangMyong type. In clinical cases, Sa-Hwang-San is proven to be effective to the following symptoms soremouth, halitosis and maxillary stagnancy due to spleen heat abdominal dropsy and powerlessness due to spleen stagnancy.

• 대한한방부인과학회지
• /
• 제19권3호
• /
• pp.175-190
• /
• 2006

Purpose : This study was conducted to investigate the in vitro inhibitory effects of heat-clearing medicinal on common bacterias in gynecology. Methods : The heat-clearing medicinals ( Trichosanthis Radix, Sophorae Fructus, Phragmitis Rhizoma, Buddleiae Flos, Bambusae Folium, Anemarrhenae Rhizoma, Celosiae Semen, Gardeniae Fructus, Prunellae Spica, Sophorae Radix, Dictamni Radicis Cortex, Coptidis Rhizoma, Gentianae Scabrae Radix, Scutellariae Radix, Phellodendri Cortex) were used in this study. Staphylococcus aureus, MRSA, Candida albicans and Gardnerella vaginalis were used for vaginal pathogenic microorganisms. Streptococcus spp., Escherichia coli HB101, Lactobacillus gasseri were used for normal vaginal florae. We evaluated antibiotic effect by the optical density and the colony test. Results : The optical density and colony test showed that Celosiae Semen, Prunellae Spica, Scutellariae Radix and Phellodendri Cortex of herbs among heat-clearing medicinal had antimircobial effect. Celosiae Semen and Prunellae Spica had antimicrobial susceptibility and selective toxicity in MRSA. Scutellariae Radix and Phellodendri Cortex had antimicrobial susceptibility and selective toxicity in Gardnerella vaginalis. Conclusion : According to the above results, we could suggest that Celosiae Semen, Prunellae Spica, Scutellariae Radix and Phellodendri Cortex among heat-clearing medicinal be available to antimicrobial agent of vaginal pathogenic microbial species in vitro.

• 동의생리병리학회지
• /
• 제19권5호
• /
• pp.1419-1426
• /
• 2005

This study was carried out to investigate the effects of herbal extracts on the skin inflammatory reactions which use the makeup preparations. In experiment 1, among the herbal ingredients of herbal extracts, ethanol extracts and 1,3-BG(Butylene Glycol) extracts of Galla Rhois showed potent radical scavenging activity, more than 91% at all concentrations, tested by DPPH (1,1-diphenyl-2-picryl-hyrazyl) method. In experiment 2, ethanol extracts of Chrysanthemi Flos, Gardenias Flos, Galla Rhois showed potent inhibitory activity of the lipopolysaccharide-induced nitric oxide(NO) production, more than 87% at $10{\mu}g/m{\ell}$, by the macrophage RAW 246.7 cells. And 1,3-BG extracts of Taraxaci Herbs, Corm Fructus, Galla Rhois showed potent inhibitory activity of nitric oxide production, more than 89% at $10{\mu}g/m{\ell}$. In experiment 3, ethanol extracts of Chrysanthemi Flos, Gardeniae Flos, Galla Rhois showed potent inhibitory effects of cyclooxygenase-II activity, more than 78% at $10{\mu}g/m{\ell}$, by using ELISA kit. And 1,3-BG extracts of Galla Rhois, Carthami Flos, Chrysanthemi Flos, Taraxaci Herba, Corm Fructus showed potent inhibitory effects of cyclooxygenase-II(COX-II) activity, more than 80% at $10{\mu}g/m{\ell}$. Therefore, 1 expect that herbal extracts, especially Galla Rhois may be used as a drug for treatment on skin inflammation and a material of the makeup preparations.

• 한방안이비인후피부과학회지
• /
• 제31권4호
• /
• pp.65-82
• /
• 2018

Objectives : The purpose of this study is 1) to explore the general tendency of the herbs or herbal formula for acne treatment in clinical practice 2) and to provide a basis for explaining the principles of acne treatment in Korean Medicine in order to make patients understand more easily based on pharmacological mechanism by analyzing the herbs or herbal formula using for acne treatment in clinical practice. Methods : In the domestic databases (Oriental medicine Advanced Searching Integrated System-OASIS, Korean Traditional Knowledge Portal-KTKP, National Discovery for Science Leader-NDSL, Research Information Sharing Service-RISS), we selected among the papers published using search terms related to "acne". Reports related to sasang constitutional medicine were excluded and a total of 23 papers were finally selected. Results : 29 prescriptions were retrieved from 23 papers. Chungan-tang (淸顔湯) notified formulas ranked first by Cheongsangbangpung- tang (淸上防風湯), Hwangnyeonhaedok-tang (黃連解毒湯), Dangguijakyaksan (當歸芍藥散) and Bipachungpe-eum (枇杷淸肺飮). Commonly used herbs are Forsythiae Fructus (連翹), Scutellariae Radix (黃芩), Gardeniae Fructus (梔子), Ligustici Rhizoma (川芎), and Angelicae Gigantis Radix (當歸). In most patients with inflammatory lesions or excessive flushing, exterior-releasing medicinal (解表藥) and heat-clearing medicinal (淸熱藥) were mainly prescribed for acne treatment, while in patients with acne accompanied hyperkeratinization, Excess sebum secretion, Dysmenorrhea or indigestion, qi-tonifying medicinal (補氣藥) or blood-tonifying medicinal (補血藥) were mainly used. KAGS (Korean Acne Grading System) was the most commonly used method for acne severity assessment. Conclusions : These findings suggest that the herbs or herbal formula for acne treatment in Korean Medicine is prescribed differently according to the cause and symptoms of acne in a unique way for control the whole body balance. Also, our findings could provide a basis for explaining the principles of acne treatment in Korean Medicine in order to make patients understand more easily based on pharmacological mechanism in clinical practice.

• 대한화장품학회:학술대회논문집
• /
• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book II
• /
• pp.1-11
• /
• 2003

Chinese herbs have been used for a long period of time and less side effects than synthesized chemical drugs. Therefore, using Chinese herbs as natural additives in cosmetics becomes popular in recent years. The methanol extracts of Scutellariae Radix, Lithospermi Radix, Lonicerae Flos, Andrographitis. Herba, Angelicae Dahuricae Radix, Ligustici Rhizoma et Radix, Hedyotis Diffusae Herba, Isatidis Folium, Magnoliae Liliflorae Flos, Forsythiae Fructus, Anmarrhenae Rhizoma, Spirodelae Herba, Gardeniae Fructus, Sophorae Flavescentis Radix, Coptidis Rhizoma, Prunellae Spica, Equiseti Hiemalis Herba, Gentianae Radix, Moutan Radicis Cortex, Fraxini Cortex, Lycii Radicis Cortex, Violae Herba, Lophatheri Herba, Matricariae chamomillae Flos, Taraxaci Herba and Scutellariae Barbatae Herba are used to test the efficiency of inhibiting acne pathogens. Twenty-six Chinese herbs are extracted by methanol, and then condensed to dried powder. These extracts are divided into water-soluble part and DMSO soluble part. These two type solutions are tested for the effect on acne pathogens by paper disc diffusion method. The results show that the substances of water soluble part which are Coptidis Rhizoma, Moutan Radicis Cortex, Scutellariae Barbatae Herba have medium to high activity of inhibiting acne pathogents, and the substances of DMSO soluble part which are Coptidis Rhizoma, Ligustici Rhizoma et Radix, Sophorae Flavescentis Radix, Moutan Radicis Cortex, Scutellariae Radix, Scutellariae Barbatae Herba also have medium to high activity of inhibiting acne pathogens. Using Chinese herbs as natural additives in cosmetics is convenience and valuable application in cosmetceutical research and development. Therefore, it is worth that re-investigation and find out the potential of Chinese herbs being use in cosmetics.

• 대한한의학방제학회지
• /
• 제21권1호
• /
• pp.80-90
• /
• 2013

Objectives : The water extract of Hwangnyeonhaedok-tang (HHT), composed of the Scutellariae Radix, Coptidis Rhizoma, Phellodendri Cortex and Gardeniae Fructus has been traditionally used to treat fever, inflammation, gastritis and hypertension in east asia. However, little is known about the ameliorative effects of HHT on atopic dermatitis. Therefore, the purpose of this study was to investigate the therapeutic effect of HHT on atopic dermatitis Methods : We investigated the inhibitory effects of HHT on the production of proinflammatory cytokines in rat peritoneal mast cells (RPMCs), on the scratching behavior in ICR mice, and on atopic dermatitis symptoms in 2,4-dinitrofluorobenzene (DNFB)-induced atopic dermatitis-like model hairless mice. Results : Levels of TNF-${\alpha}$ and IL-$1{\beta}$ increased by PMA plus A23187 co-treatment were significantly inhibited by HHT in a dose-dependent manner. HHT also inhibited the histamine release from RPMCs stimulated by compound 48/80, which promotes histamine release. The oral administration of HHT reduced the scratching behavior induced by compound 48/80 and histamine in ICR mice. Furthermore, the intradermal treatment of HHT reduced the ear edema, skin lesions, and atopic molecular marker (IgE and IL-4) in DNFB-induced atopic dermatitis model mice. Conclusions : These results suggest that HHT may be used as a potential treatment for AD as a prescription for treatment of atopic dermatitis.

• 동의신경정신과학회지
• /
• 제17권2호
• /
• pp.37-59
• /
• 2006

Objective : This study was designed to asses the effect of Hwangryunhaedoktang and herbs on Mouse neuroblastoma 2a cells damaged by hypoxia-reoxygenation. Method : Mouse neuroblastoma 2a (N2a) cells were measured by MTT assay and LDH assay after 48h hypoxia and 6h reoxygenation. Mouse neuroblastoma 2a (N2a) cells were treated by Hwangryunhaedoktang and herbs. Result : 1. Hwangryunhaedoktang was effective on LDH assay of hypoxia and reoxygenation. 2. All of herbs were generally effective on LDH assay of hypoxia and reoxygenation. In LDH assay of hypoxia, the effects of herbs depended on concentration. In MTT assay of hypoxia, Coptidis Rhizoma and Gardeniae Fructus were effective. In MTT assay of reoxygenation most of herbs were not effective. But Phellodendri Cortex was effective in high concentration. Conclusion : The results imply that Hwangryunhaedoktang and all herbs of it nay have protective effect on dementia and aging.

• 생약학회지
• /
• 제41권3호
• /
• pp.161-165
• /
• 2010

A study was performed to assess the quality equivalence of commercially available Hwang-Ryun-Hae-Dok-Tang granules from various vendors in terms of the contents of characterizing compounds. 'Hwang-Ryun-Hae-Dok-Tang'(or 'Huanglian Jiedu Tang') is the complex medicinal herbal product which is composed of four different herbal drugs; Scutellariae Radix, Coptidis Rhizoma, Phellodendri Cortex and Gardeniae Fructus. Using a high performance liquid chromatography and direct analysis in real time mass spectrometer (DART-MS), five characterizing compounds such as baicalin, baicalein, berberine, palmatine, and geniposide were analyzed to compare the variance of the contents between the products from four different vendors. Although five characterizing compounds were all successfully detected from all the products, the contents of each compound were shown to have significant difference among vendors. For real time monitoring of characterizing compounds in medicinal herbal products, DART-MS can be successfully adopted as a powerful analytical tool.