• Korean Journal of Ecology and Environment
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• v.41 no.4
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• pp.466-471
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• 2008

Little is known about DNA-level and physiological levels for health assessments of stream or river environments. Recently, comet assay, so called Single Cell Gel Electrophoresis (SCGE) is introduced for assessments of DNA damage in the medical science, food science and mammal toxicology. The comet assay is known as a biomarker which is one of the best barometers in assessing the DNA damage by oxidative stress. In this study, we conducted the comet assay using sentinel species, Zacco platypus, as one of the pre-warning alarm systems for the aquatic ecosystem health assessments and also applied it to Gap Stream as a model system. Tail extent moments in the S1 and S2 were 5.20 and 9.90 respectively and the moment was 19.89 in the S3. Statistical ANOVA in the tail moments showed a significant difference (n=75, p<0.05) between S1 and S3. Also, the proportions of DNA in the tail were 14.47, 23.64, and $30.04{\mu}m$ in the upstream (control site), midstream, downstream sites, respectively. Our results in the downstream were accord with previous studies of individual-level, population-level, and community-level in Gap Stream. Our results suggest that the comet assay may be used as an important tool for diagnosing ecological health of aquatic ecosystems in the level of DNA.

• Korean Journal of Ecology and Environment
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• v.43 no.4
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• pp.503-512
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• 2010

This study was conducted to evaluate fish fauna, species composition, and various guilds against stream orders along with analysis of fish community structure and diversity in Geum River watershed from 2005 to 2007. The total number of fish collected was 4,216 representing 12 families with 56 species. Zacco platypus was the most abundant fish species with 26% in relative abundance (RA). Korean endemic species were 24 species including Zacco koreanus, Microphysogobio yaluensis, Gobiobotia nakdongensis, and Iksookimia koreensis, etc. We also collected endangered fish species such as G. nakdongensis, Liobagrus obesus, and Pseudopungtungia nigra, etc., and their new distribution sites were found in the survey, providing some sites of the fish conservation and protection. Fish tolerance and trophic guilds analysis showed that the proportion of sensitive species, intermediate species, and tolerant species were 33.4%, 29.3%, and 37.3%, respectively and omnivores and insectivores were 48.1 % and 38.4%, respectively. Analysis of site-base study indicated that tolerant species and omnivore species were high in some polluted tributary streams (i.e., Gap and Miho stream) and sensitive and insectivore species were low. In the functional relations, expressed as simple linear regression equations, of stream order on fish metric attributes, showed that the number of species and the number of individuals increased as the stream order increases. This phenomenon was explained by greater availability of stable water volume, rich food, and higher physical habitat capacity. Such guild compositions and stream order characteristics of the river influenced the community structures, based on species diversity, dominance and evenness index in the study. This study may be used as important data in the future for comparisons of fish fauna and compositions before and after two weir (dam) constructions in the middle of Geum River by the government.

• Wind and Structures
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• v.29 no.1
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• pp.1-13
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• 2019

A numerical investigation on forces and flow around three square cylinders in side-by-side arrangement is conducted at a Reynolds number Re = 150 with the cylinder center-to-center spacing ratio L/W = 1.1 ~ 9.0, where W is the cylinder side width. The flowat this Re is assumed to be two-dimensional, incompressible, and Newtonian. The flow simulation is conducted by using ANSYS-Fluent. The flow around the three side-by-side cylinders entails some novel flow physics, involving the interaction between the gap and free-stream side flows as well as that between the two gap flows. An increase in L/W from 1.1 to 9.0 leads to five distinct flow regimes, viz., base-bleed flow (L/W < 1.4), flip-flopping flow (1.4 < L/W < 2.1), symmetrically biased beat flow (2.1 < L/W < 2.6), non-biased beat flow (2.6 < L/W < 7.25) and weak interaction flow (7.25 < L/W < 9.0). The gap flow behaviors, time-averaged and fluctuating fluid forces, time-averaged pressure, recirculation bubble, formation length, and wake width in each flow regime are discussed in detail.

• Journal of Wetlands Research
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• v.4 no.1
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• pp.63-85
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• 2002

This study was carried out to provide basic data for water environmental control and conservation, and to elucidate monthly water environmental factor, phytoplankton flora and dominant species that each stations was maked a selection of a sites by represent 3 branch-river in the Taejon area from Nov. 1997 to Dec. 1998. Range of water temperature was $2.4^{\circ}C{\sim}30.9^{\circ}C$(means of $16.02^{\circ}C{\sim}18.92^{\circ}C$), pH was 7.1~10.4(means of 7.40~8.68). BOD was generally under $5mg/{\ell}$ except Gap-chon kyo in Nov. and COD was measured highly by interfer of men at the each sites. Especially, occasion of Gap-chon kyo, data was estimated about 3~13 times higher than another sites. DO was showed 1.03~10.43ppm, SS was showed $1.0{\sim}120.0mg/{\ell}$ TN was that, upper stream was 0.63ppm, middle was 0.35ppm and then lower was 0.44ppm, upper stream was higher than lower. TP was at upper stream 0.03ppm, middle 0.05ppm, lower 0.06ppm, lower stream was higher than another. The phytoplankton flora was identified as total 230 taxa composed of 7 classes, 18 oders 35 families, 75 genara, 184 species, 42 virieties, 4 forms. There are composed of that, Chlorophyceae 94 taxa(40.9%), Bacillariophyceae 66 taxa(28.8%), Euglenophyceae 39 taxa(17.0%), Cyanophyceae 24 taxa(10.4%), Chrysophyceae 4 taxa(1.7%), Rhodophyceae 2 taxa(0.8%) Xanthophyceae 1 taxa(0.4%), composition of Spesies of each sites was Gapchon-kyo 119, Wonchon-kyo 94, Gasuwon-kyo and Mannyon-kyo 87, Ochung-kyo 78, Hyunam-kyo and Suchim-kyo 77, Moonchang-kyo 70 taxa and Sansung-kyo 58 taxa. Number of species was showed tendency of increase lower stream. Taejon-chon and youdung-chon and at the sites of confluence were presented Synedra ulna and Diatom vugare like bacillariophyceae, Gap-chon was dominated variously Oscillatoria princeps and chlorophyceae, confluence of 3 branch-river was presented Euglenophyceae.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.25 no.1
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• pp.108-116
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• 2001

The vortex tube is a simple device which separates fluid stream into a cold stream and a hot stream without any chemical reaction. The process of energy separation in the vortex tube has caused a great deal of interest. Although many studies on energy separation in the vortex tube using air as the working fluid have been made so far, few experimental studies treated energy separation for incompressible fluid. So, an experimental study for the energy separation in the vortex tube using the water which is essentially an incompressible fluid is presented. When working fluid is the water, the best geometric values of nozzle area ratio and number of nozzle holes are 0.155, 6 respectively. These geometric values are showed by the similar values which are presented by compressible fluid as working fluid. But hot side mass fraction of which maximum temperature drop is happened are different from compressible fluid.

• Korean Journal of Ecology and Environment
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• v.43 no.1
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• pp.24-34
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• 2010

This study was carried out to analyze some influences on ecological health conditions, threaten by various stressors such as physical, chemical and biological parameters. We collected samples in 2008 from three zones of upstream, midstream and downstream, Gap Stream. We applied multi-metric fish assessment index (MFAI), based on biotic integrity model to the three zones along with habitat evaluations based on Qualitative Habitat Evaluation Index (QHEI). We also examined fish fauna and compositions, and analyzed relations with MFAI values, QHEI values, and various guild types. Chemical parameters such as oragnic matter (BOD, COD), nutrients (TP, $NH_3$-N), coli-form number (as MPN), and suspended solids (SS) were analyzed to identify the relationship among multiple stressor effects. Using the sentinel species of Zacco platypus, the population structures and condition factors were analyzed along with DNA damages related with genotoxicant effects by comet assay. This study using all these parameters showed that stream condition was degraded along the longitudinal gradient from upstream to downstream, and the downstream, especially, was impacted by nutrient enrichment and toxicant exposure from the point source, wastewater treatment plant. Overall results indicated that our approaches applying various parameters may be used as a cause-effect technique in the stream health assessments and also used as a pre-warning tool for diagnosis of ecological degradation.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.20 no.3
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• pp.1061-1073
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• 1996

Vortex tube is a simple device which splits a compressed gas stream into a cold stream and a hot stream without any chemical reactions. The phenomena of energy separation taking place in a vortex tube has been investigated experimentally. Recently, vortex tube is widely used to local cooler of industrial equipments and air conditioner of special purpose. In this study, experimental study on vortex tube efficiency was performed with various cold end orifices and nozzles type. The experimental results indicate that there is an optimum diameter of cold end orifice and nozzle type for the best cooling performance. The variation of the maximum wall temperature along the vortex tube surface provides useful information about the location of the stagnation point of the flow field at the axis of the vortex tube. The similarity relation for the prediction of the temperature of the cold exit air was obtained.

• Tribology and Lubricants
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• v.17 no.5
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• pp.391-398
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• 2001

Two dimensional slow viscous flow around two counter-rotating equal cylinders is investigated based on Stokes'approximation. An exact formal expression of the stream function is obtained by using the bipolar cylinder coordinates and Fourier series expansion. From the stream function obtained, the streamline patterns around the cylinders are shown and the pressure distribution in the flow field is determined. By integrating the stress distributions on the cylinder, the force and the moment exerted on the cylinder are calculated. The flow rate through the gap between the two cylinders is also determined as the distance between two cylinders varies. Special attention is directed to the case of very small distance between two cylinders concerned with the lubrication theory and the minimum pressure is calculated to explain a possible cavitation.

• Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
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• 2000.06a
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• pp.142-149
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• 2000

Two dimensional slow viscous flow around two counter-rotating equal cylinders is Investigated based on Stokes' approximation. An exact formal expression of the stream function is obtained by using the bipolar cylinder coordinates and Fourier series expansion. From the stream function obtained, the streamline patterns around the cylinders are shown and the pressure distribution In the flow field is determined. By Integrating the stress distribution on the cylinder, the force and the moment exerted on the cylinder are calculated. The flow rate through the gap between the two cylinders is determined as the distance between two cylinders vary. It Is also revealed that the velocity at the far field has finite non-zero value. Special attention is directed to the case of very small distances between two cylinders by way of the lubrication theory.

• Tribology and Lubricants
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• v.18 no.6
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• pp.411-417
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• 2002

Two dimensional slow viscous flow between a rotating cylinder and a translating plate is investigated using Stokes' approximation. An exact formal expression of the stream function is obtained by using the bipolar cylinder coordinates and Fourier series expansion. From the stream function obtained, the streamline patterns are shown and the pressure distribution in the flow field is determined. By integrating the stress distributions on the cylinder, the farce and the moment exerted on the cylinder are calculated. The flow rate through the gap between the cylinder and the plate is also determined as a function of the distance between the cylinder and the plate. Special attention is directed to the case of very small distance between the cylinder and the plate concerned with the lubrication theory and the minimum pressure is calculated to explain a possible cavitation.