• Clinical and Experimental Reproductive Medicine
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• 제21권2호
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• pp.201-206
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• 1994

Down syndrome is one of the major chromosomal anomalies in Korea. To decrease incidence of Down syndrome, antenatal diagnosis is essential. At present, antenatal diagnosis of Down syndrome is done by karyotyping from chorionic villus sampling, amniocentesis, and cordocentsis. All these methods have some problems such as a risk of abortion, a long waiting time, difficulties in sampling, and so on. The aim of study was to confirm that PCR(Polymerase Chain Reaction) using D21S11 primer could be a diagnostic tool for Down syndrome. PCR using D21S11 primers with $^{32}P$ labeling at 5' end was done in 21 cases of DNA from 21 Trisomy and 20 cases of DNA from normal karyotype. PCR product was running for 10 hours on the 6% polyacrylamide gel under 1,000 V or for 8 hours under 1,500 V. After X-ray film exposure, it was read by densitometry. Normal group showed 1: 1 band or single band. 21 Trisomy group showed 1.3-2: 1 band or 2.3 times of density compared to normal single band or 3 bands. This method gave the result within 24 hours. It can be an useful diagnostic tool to detect 21 Trisomy antenatally, especially in late pregnancy, and in preimplantation diagnosis.

• 한국환경농학회:학술대회논문집
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• 한국환경농학회 2009년도 정기총회 및 국제심포지엄
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• pp.93-115
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• 2009

There are growing public concerns over crop and food safeties due to the elevated levels of heavy metals grown in contaminated soil. Heavy metals are classified as the chemical harmful risks for crop and food safety. With implementation of GAP, crop safety is controlled by many regulatory options for soil, irrigation water and fertilizers. Any attempt to retard the metal uptake by crops may be the best protocol to secure crop and food safety. This article reviews the management strategies for heavy metals in view of crop safety in Korea and demonstrates results from the field experiments to retard metal translocation from soil to crops by using chemical amendments and soil layer management methods. Major source of soil pollution by heavy metals has been related with mining activities. Risk assessment revealed that rice consumption and groundwater ingestion in the abandoned mining areas were the major exposure pathways for metals to human and the heavy metal showed the toxic effects on human health. Chemical amendments such as lime and slag retarded Cd uptake by rice (Oryza sativa L.) by increasing soil pH, lowering the phytoavailable Cd concentration in soil solution, immobilizing Cd in soil and converting the available Cd fractions into non-available fractions. The soil layer management methods decreased the Cd uptake by 76% and Pb by 60%. Either reversing the surface layer with subsurface layer or immobilization of metals with layer mixing with lime was considered to be the practical option for the in-situ remediation of the contaminated paddy soils. Combination of chemical soil amendments and layer management methods was efficient to retard the metal bioavailability and thus to secure crop safety for heavy metals. This protocol seems to be cheap, relatively easy to practice and practical in the agricultural fields. However, a long term monitoring work should be followed to verify the efficiency of this protocol.

• 한국발생생물학회:학술대회논문집
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• 한국발생생물학회 1998년도 제4차 학술발표대회 및 정기총회
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• pp.12-18
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• 1998

Tremendous progress has been made over the past quarter-century studying the genetics of gametogenesis and the resulting gametes and embryos. Studies merging molecular techniques and conventional cytogenetics are now beginning to bridge the gap between what we have learned about the meiotic process in males and females and what we know of the mitotic chromosomes of zygotes. Numerical abnormalities in sperm, oocytes and embryo can now diagnosed by fluorescence in situ hybridization (FISH). "At risk" couples can, therefore, have only unaffected embryos replaced in the sterus and avoid the possibility of terminating a pregnancy that might only be diagnosed as affected later gestation. Single-cell genetic analysis has also provided powerful tools for studying genetic defects arising during early human development. Recent studies of sperms, oocytes and cleavage-stage human embryos have revealed an unexpectedly high incidence. These genetic abnormalities are likely to contribute to early pregnancy loss and have important implications for improving pregnancy rates in infertile couples by assisted reproduction. The widespread use of preimplantation genetic diagnosis (PGD) awaits further documentatio of safety and accuracy. Other issues also must be addressed. First, the ethical issues regarding germ cell and embryo screening must be addressed including what diseases are serious enough to warrant the procedure. Another concern is the use of this technology for non-genetic disorders such as gender selection. Finally, the experimental nature of these procedure must continually be discussed with patients, and long-term follow-up studies must be undertaken. Development of more accurate and less expensive assays coupled with improved assisted reproductive technology success rates may make PGD a more widely use clinical tool. The future awaits these development.velopment.

• 대한임상검사과학회지
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• 제48권2호
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• pp.137-143
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• 2016

본 연구는 22명의 남자 아마추어 선수를 대상으로 100 km 울트라 마라톤에서의 전해질 대사 반응의 안전성을 평가하기 위해 실시하였다. 대상자의 평균 연령은 $50.91{\pm}4.77$세, $VO_2max$는 $48.19{\pm}6.43mL/kg/min$, 그리고 평균 경기 완주시간은 $829.64{\pm}89.31$분이었다. 혈액검사에 포함된 전해질 지표로는 칼슘, 무기인, 마그네슘, 소디움, 포타슘, 염소, 총이산화탄소, 음이온 차이, 혈장량 변화와 삼투압 농도를 경기 전, 50 km 지점, 경기 종료 직후 측정하였다. 본 연구결과 소디움 농도는 경기 전보다 50 km 지점에서 감소하였고 50 km 지점보다 100 km 지점에서 감소하였으나, 고나트륨혈증 및 저나트륨혈증은 나타나지 않았다. 100 km 울트라 마라톤 경기에서 다른 모든 전해질 지표들의 거리별 변화는 참고범위 내에서 이루어졌으며, 혈장량 변화는 나타나지 않았다. 결론적으로 아마추어 지구력 선수들의 전해질 대사에 있어서 경기중 통계적인 차이는 있었으나 임상적으로 의미 있는 삼투압 농도와 전해질의 변화는 나타나지 않았으며 안전한 경기로 나타났다.

• Journal of Welding and Joining
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• 제31권5호
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• pp.35-40
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• 2013

In this paper, we propose the Tangible Virtual Reality Representation Method to using haptic device and feature to morphology of created bead from Flux Cored Arc Welding. The virtual reality was started to rising for reduce to consumable materials and welding training risk. And, we will expected maximize virtual reality from virtual welding training. In this paper proposed method is get the database to changing the input factor such as work angle, travelling angle, speed, CTWD. And, it is visualization to bead from extract to optimal morphological feature information to using the Neural Network algorithm. The database was building without error to extract data from automatic robot welder. Also, the Neural Network algorithm was set a dataset of the highest accuracy from verification process in many times. The bead was created in virtual reality from extract to morphological feature information. We were implementation to final shape of bead and overlapped in process by time to using bead generation algorithm and calibration algorithm for generate to same bead shape to real database in process of generating bead. The best advantage of virtual welding training, it can be get the many data to training evaluation. In this paper, we were representation bead to similar shape from generated bead to Flux Cored Arc Welding. Therefore, we were reduce the gap to virtual welding training and real welding training. In addition, we were confirmed be able to maximize the performance of education from more effective evaluation system.

• 보건행정학회지
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• 제30권2호
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• pp.199-210
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• 2020

Background: Based on the importance of ceasing smoking programs to control the regional disparity of smoking behavior in Korea, this study aims to reveal the variation of smoke rate and determinants of it for 229 provinces. An evaluation of the relative efficiency of the cease smoking program under the consideration of regional characteristics was followed. Methods: The main sources of data are the Korean Statistical Information Service and a national survey on the expenditure of public health centers. Multivariate regression is performed to figure the determinants of regional variation of smoking rate. Based on the result of the regression model, clustering analysis was conducted to group 229 regions by their characteristics. Three clusters were generated. Using data envelopment analysis (DEA), relative efficiency scores are calculated. Results from the pooled model which put 229 provinces in one model to score relative efficiency were compared with the cluster-separated model of each cluster. Results: First, the maximum variation of the smoking rate was 16.9%p. Second, sex ration, the proportion of the elder, and high risk drinking alcohol behavior have a significant role in the regional variation of smoking. Third, the population and proportion of the elder are the main variables for clustering. Fourth, dissimilarity on the results of relative efficiency was found between the pooled model and cluster-separated model, especially for cluster 2. Conclusion: This study figured regional variation of smoking rate and its determinants on the regional level. Unconformity of the DEA results between different models implies the issues on regional features when the regional evaluation performed especially on the programs of public health centers.

• 한국산학기술학회논문지
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• 제14권10호
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• pp.5115-5122
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• 2013

본 논문은 급경사지(산사태 및 사면붕괴, 축대 등)붕괴 등으로 인하여 많은 생명과 재산피해 방지를 목적으로 붕괴 위험성을 감지하고 신속히 대응을 할 수 있도록 하기 위하여 u-IT기반의 급경사지 붕괴예측 감시용 실시간 모니터링 시스템을 개발하였다. 급경사지붕괴 감시에 중요한 계측기로서 강우량 계측기, 간극수압 계측기, 지표변위 계측기, 지중경사 계측기, 함수비계측기, 영상분석 계측기 등을 선정하고 테스트베드에 적용하였다. 각 계측기의 신뢰성 검증에 필요한 동작기능 및 성능확인은 현장에 설치된 계측기 별로 실험을 통하여 확인하였다. 본 연구에서 개발한 급경사지붕괴 감지를 위한 USN기반의 실시간 급경사지 모니터링 시스템을 급경사지 붕괴감지뿐만 아니라 도로변 절개사면과 암반사면 등에 상시계측을 통하여 붕괴위험 예측에도 적용할 수 있으므로 인명피해와 재산피해를 최소할 수 있을 것으로 판단되며, 이 시스템은 본 연구의 시범적용 결과를 바탕으로 급경사지 전역에 확산될 계획에 있다.

• 인터넷정보학회논문지
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• 제21권5호
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• pp.75-85
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• 2020

The application of Internet technology has created enormous impact on banking sector with the implementation of many techno-oriented services like Internet banking, EFT, branchless banking, Automated Clearing House (ACH) transactions etc. Study of customer's attitude in terms of trust, perceived risk and ease of use of a particular technology is as an important parameter for acceptance or rejection of a technology. To explore the customers'attitude for Internet banking this research is undertaken. The research is carried out in Pokhara valley which is the second largest city and tourism capital of Nepal. The study employs descriptive research design with stratified sampling procedure for eight top commercial banks. A set of 25 customers is taken from each selected 8 banks making a sample size of 200 respondents. A fixed set of question related to demographic factors is provided personally or by visiting the location of the customers of Internet banking service and collected accordingly. Reliability test is performed using Cronbach's alpha and data is analyzed using inferential statistics to present the results of the study. This study provides knowledge on the current scenario of Internet banking and helps banks in cost saving, mass customization, product innovation, improved marketing and communication. This study is very important for financial institutions like banks, government agencies and business houses to understand the perception of customers towards Internet banking and technology as a whole. The study also supplements the gap in literature on technology and banking in Nepal and serves as an important knowledge base.

• 디지털융복합연구
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• 제15권1호
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• pp.199-208
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• 2017

본 연구는 기술신용정보의 기술금융공여자가 신뢰할 수 있는 기술신용정보의 구성요소와 등급산출체계를 분석하고 이를 토대로 기술금융 공급확대를 유인할 수 있는 최적의 기술신용평가시스템을 도출하는 것이다. 기술평가등급과 신용평가등급의 결합비율 변화를 통해 최대 AUROC 값이 되는 최적화된 기술신용평가등급을 산출하고 기존의 신용평가등급 및 체계 간의 격차 시뮬레이션을 통해 기술신용평가등급과 신용평가등급 간 대체가능성을 검증해 본 후 금융기관이 활용할 수 있는 등급체계를 제시하였다. 연구결과, 기업 규모별, 업종별로 동일하게 신용평점 : 기술평점의 가중치 결합비율 70% : 30% 일 때 AUROC가 가장 높게 나타났다. 본 연구를 통해 기술신용등급의 부도 유의성이 신용등급 또는 기술등급보다 향상된 결과를 확인함에 따라 기술신용평가정보가 신용등급을 대체 적용 가능성을 발견하였고 나아가서 금융기관에서 여신의사결정 시 기술평가정보와 신용평가정보가 최적화 결합된 기술신용등급을 이용하여 정교한 리스크 관리도 가능함을 시사하고 있다.

• STI Policy Review
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• 제4권1호
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• pp.94-118
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• 2013

Innovation plays a large role in green growth. While it is a widely accepted view that, without innovation, it would be very difficult and costly to address major environmental issues, innovation itself tends to be constrained by limited access to eco-financing and is inherently risky, often requiring a long-term horizon. Although global consensus is more or less established as to the urgency and necessity of accelerating green innovation, the quality and quantity of financing in this area is largely insufficient, with increasing funding gaps in many countries. A new financial mechanism is urgently needed in order to re-orient financial flow and enable innovators to overcome the valleys of death that occur throughout the innovation cycle. A number of different modalities exist in financing the commercialisation of eco-innovation. Existing mechanisms have not been as successful as expected, revealing critical limits to furthering certain types of projects that are essential for economic and environmental progress. Experts' estimations have shown that the funding gap will widen in the coming years as demand for clean energy and green infrastructure rises, and as green technologies and innovation develop faster than the market for it can develop. Against this backdrop, the main purpose of this research is threefold: to identify issues and problems regarding current means of funding for eco-innovation and green projects; to provide insight into securing longterm green financing by looking at European cases; and ultimately to suggest policy implications for designing and implementing eco-specific financial instruments, focusing on governments' roles in sustainable financing for eco-innovation. This study analyses different models of financing mechanisms, a mix of public and private funds, in view of suggesting conditions for the sustainable financing of green projects, especially for large-scale high-risk projects. Based on the findings from the analyses of mechanisms and the shortcomings of the existing funding modalities, this study ultimately suggests policy implications for effectively supporting the commercialisation of eco-innovation.