• BMB Reports
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• 제33권6호
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• pp.525-530
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• 2000

The nerve growth factor (NGF) induces neuronal differentiation and neurite outgrowth of PC12 cells, whereas epidermal growth factors (EGF) stimulate growth and proliferation of the cells. In spite of this difference, NGF-or EGF-treated PC12 cells share various properties in cellular-signaling pathways. These include the activation of the phosphoinositide (PI)-3 kinase, 70 kDa S6 kinase, and in the mitogen-activated protein (MAP) kinase pathway, following the binding of these growth factors to intrinsic receptor tyrosine kinases (RTKs). Therefore, many studies have been attempted to access the critical signaling events in determining the differentiation and proliferation of PC12 cells. In this study, we investigated the cytosolic phospholipase $A_2$ ($cPLA_2$) in neurite behavior in order to identify the differences of signaling pathways between the NGF-induced differentiation and the EGF-induced proliferation of PC12 cells. We have showed here that the $cPLA_2$ was translocated from cytosol to membrane only in NGF-treated cells. We also demonstrated that this translocation is associated with NGF-induced activation of phospholipase $C-{\gamma}(PLC-{\gamma})$, which elevates intracellular $Ca^{2+}$ concentration. These results reveal that the translocation of $cPLA_2$ may be a requisite event in the neuronal differentiation of PC12 cells. Various phospholipase inhibitors were used to confirm the importance of these enzymes in the differentiation of PC12 cells. Neomycin B, a PLC inhibitor, dramatically inhibited the neurite outgrowth, and two distinct $PLA_2$ inhibitors, 4-bromophenacyl bromide (BPB) and arachidonyltrifluoro-methyl ketone ($AACOCF_3$) also suppressed the neurite outgrowth of the cells, as well Taken together, these data indicated that $cPLA_2$ is involved in NGF-induced neuronal differentiation and neurite outgrowth of PC12 cells.

• Biomolecules & Therapeutics
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• 제8권4호
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• pp.325-331
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• 2000

The present study was designed to assess the role of spinal adenosine $A_2$ receptor in the regulation of cardiovascular functions such as mean arterial pressure (MAP) and heart rate (HR) in male Sprague-Dawley rats. Rats (250~300 g) were anesthetized with urethane and paralyzed with d-tubocurarine and artificially ventilated. blood pressure and HR were continuously monitored via a femoral catheter connected to a pressure transducer and a polygraph. Drugs were administered intrathecally using injection cannula through guide cannula which was inserted inthrathecally at lower thoracic level through a puncture of an atlantooccipital mombrane. Intrathecal injection of an adenosine $A_2$ receptor agonist, 5'-(N-cyclopropyl)-carboxamaidoadenosine (CPCA; 1, 2 and 3 nmol, respectively), produced a dose-dependent decrease in MAP and HR. Pretreatment with $N^{G}$-nitro-L-arginine methyl ester (L-NAME), a nitric oxide synthase inhibitor or 10 nmol of MDL-12,330, an adenylate cyclase inhibitor blocked significantly the depressor and bradycardic effect of 2 nmol of CPCA. But, Pretreatment with 3 nmol of bicuculline, gamma-aminobutyric acid A (GAB $A_{A}$) receptor antagonist, or 50 nmol of 5-aminovaleric acid, GAB $A_{B}$ receptor antagonist did not inhibit the depressor and bradycardic effect of 2 nmol of CPCA. These results indicate that adenosine $A_2$ receptor in the spinal cord plays an inhibitory role in the regulation of cardiovascular function and that the depressor and bradycardic action of adonosine $A_2$ receptor are mediated via the synthesis of nitric oxide and the activation of adenylate cyclase in the spinal cord of rats.s.s.s.

• 방송공학회논문지
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• 제22권3호
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• pp.327-338
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• 2017

노출 합성은 두 장 이상의 서로 다른 노출 값을 갖는 좁은 동적 영역 영상을 합쳐 한 장의 넓은 동적 영역을 갖는 결과 영상을 생성하는 알고리듬이다. 본 논문은 블록기반의 지역적 특성을 고려한 노출 조정 기법과 개선된 채도 특성 요소를 이용해 가중치 맵을 생성하는 알고리듬을 제안한다. 제안하는 노출 조정 기법은 인간시각체계의 특성을 고려하여 입력 영상의 노출 값을 보정함으로써 노출 합성 결과 영상 내의 세밀한 부분을 효과적으로 보존한다. 개선된 채도 영상은 입력 영상 내의 포화 영역을 효과적으로 반영한 가중치맵을 생성한다. 본 논문은 기존의 대표적인 노출 합성 알고리듬과의 주관적 화질과 MEF-SSIM, 수행 시간 비교를 통해 제안하는 알고리듬의 우수성을 입증하였다.

• 대한원격탐사학회지
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• 제35권1호
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• pp.29-37
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• 2019

Synthetic Aperture Radar (SAR) observations are powerful tools to monitor surface's displacement very accurately, induced by earthquake, volcano, ground subsidence, glacier movement, etc. Especially, radar interferometry (InSAR) which utilizes phase information related to distance from sensor to target, can generate displacement map in line-of-sight direction with accuracy of a few cm or mm. Due to decorrelation effect, however, degradation of coherence in the InSAR application often prohibit from construction of differential interferogram. Offset tracking method is an alternative approach to make a two-dimensional displacement map using intensity information instead of the phase. However, there is limitation in that the offset tracking requires very intensive computation power and time. In this paper, efficiency of parallel computing has been investigated using high performance computer for estimation of glacier velocity. Two TanDEM-X SAR observations which were acquired on September 15, 2013 and September 26, 2013 over the Narsap Sermia in Southwestern Greenland were collected. Atotal of 56 of 2.4 GHz Intel Xeon processors(28 physical processors with hyperthreading) by operating with linux environment were utilized. The Gamma software was used for application of offset tracking by adjustment of the number of processors for the OpenMP parallel computing. The processing times of the offset tracking at the 256 by 256 pixels of window patch size at single and 56 cores are; 26,344 sec and 2,055 sec, respectively. It is impressive that the processing time could be reduced significantly about thirteen times (12.81) at the 56 cores usage. However, the parallel computing using all the processors prevent other background operations or functions. Except the offset tracking processing, optimum number of processors need to be evaluated for computing efficiency.

• Journal of Microbiology and Biotechnology
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• 제18권6호
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• pp.1170-1178
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• 2008

The cyclopentenone prostaglandins (cyPGs) prostaglandin $A_1$ ($PGA_1$) and 15-deoxy-${\Delta}^{12,14}$-prostaglandin $J_2$ (15d-$PGJ_2$) have been reported to exhibit antiinflammatory activity in activated monocytes/macrophages. However, the effects of these two cyPGs on the expression of cytokine genes may differ. In this study, we investigated the mechanism of action of $PGA_1$ in lipopolysaccharide (LPS)-induced expression of inter leu kin (IL)-10 mRNA in mouse peritoneal macrophages. 15d-$PGJ_2$ inhibited expression of LPS-induced IL-10, whereas $PGA_1$ increased LPS-induced IL-10 expression. This synergistic effect of $PGA_1$ on LPS-induced IL-10 expression reached a maximum as early as 2 h after simultaneous $PGA_1$ and LPS treatment ($PGA_1$/LPS), and did not require new protein synthesis. The synergistic effect of $PGA_1$ was inhibited by GW9662, a specific peroxisome proliferator-activated receptor ${\gamma}(PPAR{\gamma})$ antagonist, and Bay-11-7082, a NF-${\kappa}B$ inhibitor. The extracellular signal-regulated kinases (ERK) inhibitor PD98059 increased the expression of $PGA_1$/LPS-induced IL-10 mRNA, rather than inhibiting the IL-10 expression. Moreover, $PGA_1$ inhibited LPS-induced ERK phosphorylation. The synergistic effect of $PGA_1$ on LPS-induced IL-10 mRNA and protein production was inhibited by p38 inhibitor PD169316, and $PGA_1$ increased LPS-induced p38 phosphorylation. In the case of stress-activated protein kinase/c-Jun $NH_2$-terminal kinase (SAPK/JNK), the SAPK/JNK inhibitor SP600125 did not inhibit IL-10 mRNA synthesis but inhibited the production of IL-10 protein remarkably. These results suggest that the synergistic effect of $PGA_1$ on LPS-induced IL-10 expression is NF-${\kappa}B$-dependent and mediated by mitogen-activated protein (MAP) kinases, p38, and SAPK/JNK signaling pathways, and also associated with the $PPAR{\gamma}$ pathway. Our data may provide more insight into the diverse mechanisms of $PGA_1$ effects on the expression of cytokine genes.

• Journal of Radiation Protection and Research
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• 제39권1호
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• pp.21-29
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• 2014

삼축분광장치는 물질을 이루고 있는 자성 원소들의 거동, 즉 스핀 동역학을 측정하는데 적합한 장치로, 연구용 원자로 '하나로'에는 국내 유일의 냉중성자 삼축분광장치가 최근 설치되었다. 삼축분광장치는 중성자 빔을 제어하는 중성자광학 부품과 중성자 빔으로 인해 발생하는 방사선에 대한 차폐체로 이루어지며 이러한 부품은 수십 톤 중량의 기계구조물을 이룬다. 방사선 차폐는 중성자 빔 경로 이외의 방향으로 진행하는 중성자와 감마선을 효과적으로 막아 신호대 잡음비를 향상시키는 역할을 하며 구조물 내부의 방사화된 부품으로부터 발생하는 감마선을 차폐하여 장치 이용자의 피폭선량을 최소화한다. 그런데 설치된 냉중성자 삼축분광장치의 차폐체 중 전면부의 고하중으로 인해 장치 운영상 여러 가지 문제점이 발생, 전면 세그먼트 차폐체의 하중을 줄이는 구조개선이 불가피하였다. 이에 MCNPX 모의계산을 통해 냉중성자 삼축분광장치의 차폐체 최적화에 필요한 개선방향을 검토하였다. 상부 차폐체의 폴리에틸렌과 납의 추가 설치를 통해 전면 블록 차폐체 하중을 줄일 수 있는 최적 길이를 확인하였다. 그 결과, 전면 블록 차폐체의 높이 20%가 제거된 경우, 구조변경 전 대비 차폐체 상부에서 70% 수준의 감마선속이 나타남을 확인하였다. 하지만 높이를 줄일수록 전면 블록 차폐체의 하중을 줄일 수 있기 때문에, 차폐블록을 추가 제거하고 이에 대한 차폐능을 보상해 줄 방안으로 상부 납 차폐체의 위치 변화에 따른 중성자속과 감마선속을 예측해 보았다. 전면 블록 차폐체 높이의 35% 제거하고 상부 납 차폐체를 최하단부에서 10 cm에 설치한 경우, 전면 블록 차폐체 상부에서 감마선속이 각각 25%, 18% 증가하였다. 증가한 감마선속의 영향을 파악하기 위해 MCNPX 모의계산을 통해 공간의 감마선속 분포를 가시화하였다. 증가한 감마선속은 상부로 향하는 방향성을 띄며 이동하면서 소멸하여 검출기에 이르기 전에 낮아져 검출기와 실험자의 위치에 영향을 끼칠 수 없다고 판단하였다. 그래서 중성자속 및 감마선속과 고하중 문제를 동시에 해결할 수 있는 최적화 조건으로 차폐체 높이가 35% 제거되고 상부 납 차폐체가 10 cm 위치에 있는 경우를 선정하였다. 이 결과를 바탕으로 구조개선 작업을 실시하였으며 열형광선량계를 이용하여 콘크리트 차폐블록 외부에서 중성자와 감마선량을 측정하였다. 측정된 중성자 선량은 0.21 ${\mu}Svhr^{-1}$, 감마선량은 3.69 ${\mu}Svhr^{-1}$로 설계기준을 만족하였으며 피폭으로부터 실험자의 안전성을 확인하였다.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2009년도 학술발표회 초록집
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• pp.939-942
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• 2009

본 연구는 경북지역의 11개 관측소와 인근 9개의 관측소의 강우자료를 이용하여 경북지역에 한정된 확률강우량도를 작성하였다. 최근 행정구역별 치수계획의 수립이 빈번해지고 소규모 유역의 개발로 인한 홍수량 산정 등이 빈번해지고 있다. 그러나 대부분이 강우관측소가 유역내에 위치해 있지 않고 인접한 기상관측소의 자료를 이용하고 있는 실정이고, 공공기관이나 실무를 수행함에 있어 유역의 강우량 적용에 있어 소규모 유역의 강우량이 지점강우량에 의해 결정되므로 어느 정도의 편차를 보이는지 추정이 사실상 곤란하였다. 따라서 본 연구에서는 경북지역에 한정하여 지점강우량을 빈도해석하여 확률강우량도를 작성하여 강우관측소가 인접하지 않아도 소규모 유역의 확률강우량의 근사치를 추정하여 지점빈도해석과 비교할 수 있도록 확률강우량도를 작성하였다. 경북지역인근 강우관측소의 자료를 강우 분석하여 확률분포형을 선정한 결과 거창, 구미, 대구, 문경, 밀양, 봉화, 안동, 영덕, 영주, 울산, 의성, 제천 충주, 추풍령, 합천은 Gumbel 분포가 적합한 것으로 나타났으며, 보은은 2변수 Log-Gumbel 분포가 적합한 것으로 나타났으며, 영천, 울진, 태백은 Gamma 분포가 적합한 것으로 나타나고 포항은 GEV 분포가 적합한 것으로 나타났다.

• 대한수학회지
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• 제42권1호
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• pp.111-127
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• 2005

Let B and S be the unit ball and the unit sphere in $\mathbb{C}^n$, respectively. Let ${\sigma}$ be the normalized Lebesgue measure on S. Define the Privalov spaces $N^P(B)\;(1\;<\;p\;<\;{\infty})$ by $$N^P(B)\;=\;\{\;f\;{\in}\;H(B) : \sup_{0 where H(B) is the space of all holomorphic functions in B. Let ${\varphi}$ be a holomorphic self-map of B. Let ${\mu}$ denote the pull-back measure ${\sigma}o({\varphi}^{\ast})^{-1}$. In this paper, we prove that the composition operator $C_{\varphi}$ is metrically bounded on $N^P$(B) if and only if ${\mu}(S(\zeta,\delta)){\le}C{\delta}^n$ for some constant C and $C_{\varphi}$ is metrically compact on $N^P(B)$ if and only if ${\mu}(S(\zeta,\delta))=o({\delta}^n)$ as ${\delta}\;{\downarrow}\;0$ uniformly in ${\zeta}\;\in\;S. Our results are an analogous results for Mac Cluer's Carleson-measure criterion for the boundedness or compactness of $C_{\varphi}$ on the Hardy spaces $H^P(B)$.

• 한국주거학회:학술대회논문집
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• 한국주거학회 2003년도 정기총회 및 추계학술대회
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• pp.295-299
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• 2003

The development of rapid science technology and data communication technology in 1990 was infiltrated into our real lives deeply and drew forth various changes in various sides. According to that with the new definition of the character of space the users' patterns in the space were changed. We come to have a turning point of change with the introduction of personal computer and digital revolution late in the 20th century. Accordingly, this study is to analyze the connected structure of the space on a basis of the apartment model house since 2000 in order to investigate the aspect of changing the space according to the era of informationization. This analysis of space structure makes the characteristics in the era of informationization objectified. And it will understand the distinct change of space by grasping the relations with the connection of space, and predict the direction that the dwelling space heads for the future.

• 열처리공학회지
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• 제28권4호
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• pp.171-180
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• 2015

The variation in microstructure and texture during continuous annealing was examined in a series of 1.6% Mn-0.1% Cr-0.3% Mo-0.005% B steels with carbon contents in the range of 0.010 to 0.030%. It was found that microstructure of hot band consisted of ferrite and pearlite as a consequence of high coiling temperature, and eutectoid carbon content was between 0.011% and 0.016%. Martensite ranged in volume fraction from 1.5% to 4.0% when annealed at $820{\circ}C$ according to the typical continuous annealing cycle. The critical martensite content for the continuous yielding was about 4% from stress-strain curves. The continuous yielding was obtained in the 0.030% carbon steel and 0.010% to 0.020% carbon steels revealed some yield point elongation ranging from 0.8% to 2.2% in as-annealed conditions. Higher tensile strength in the higher carbon steel is due to both increase in the martensite volume fraction and ferrite grain refinement. Decreasing the carbon content to 0.01% strengthened the intensities of ${\gamma}$-fiber textures, resulting in the increase in the $r_m$ value, which was caused by the lower volume fraction of martensite. The higher carbon steels showed the lower $r_m$ value of about 1.0.