• Journal of the Korean Applied Science and Technology
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• v.10 no.1
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• pp.93-101
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• 1993

This study aimed to search for separate and identify of Quercus acutissima CARRUTHERS tannins. Tannins were extracted with methanol and ethylacetate from acorn powder and identified TLC, UV spectrum, HPLC, IR, GC/MS, and $^{1}H$ NMR. Three spots($R_{f}$ 0.94, 0.84 and 0.29) detected on TLC. These spots gave dark blue color fairly on spraying with 0.3% potassium ferricyanid${\cdot}$0.3% ferricchloride reagent, and these tannins identified as gallic acid, caffeic acid and ellagic acid by UV spectrum, HPLC, IR, GC/ MS, and $^{1}H$ NMR.

• Korean Journal of Pharmacognosy
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• v.2 no.1
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• pp.41-42
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• 1971

Pale yellow microneedles$(m.p.\;228{\sim}230^{\cric})$ and colorless microneedles$(m.p.\;235{\sim}237^{\circ})$ were respectively obtained from the leaves of Cornus kousa $B_{UERGERI}$. These two substances were as iso-quercitrin and gallic acid by paper partition and thin-layer chromatographies and physicochemical tests.

• Bulletin of the Korean Chemical Society
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• v.31 no.4
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• pp.1031-1034
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• 2010

• Bulletin of the Korean Chemical Society
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• v.33 no.6
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• pp.2051-2054
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• 2012

• Archives of Pharmacal Research
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• v.18 no.3
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• pp.203-205
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• 1995

A new $4,5,8-trihydroxy-{\alpha}-tetralone-5-O-{\beta}-D-glucoside$ along with 1,4,8-trihydroxynaphthalene1-O-{\beta}-D-glucoside$ and gallic acid were isolated from the roots of Juglans mandshurica. Their structures were elucidated on the basis of spectroscopic studies.

• Korean Journal of Food Science and Technology
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• v.51 no.5
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• pp.486-491
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• 2019

Phenolic compounds and antioxidant activities of ethanol extracts from barley sprouts were evaluated in this study. Barley sprouts were extracted using water and ethanol in various concentration (25, 50, and 75%) using reflux extraction methods. Ultra performance liquid chromatography (UPLC) analysis showed that barley sprouts are mainly composed of rutin, gallic acid, ferulic acid, and ${\rho}$-coumaric acid. The 75% ethanol extracts had higher total polyphenol contents ($44.01{\pm}1.32mg/g$) and total flavonoid contents ($102.96{\pm}2.49mg/g$). 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity ($EC_{50}$ value: $1.65{\pm}0.02mg/mL$) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical scavenging activity ($EC_{50}$ value: $1.67{\pm}0.02mg/mL$) of the 75% ethanol extracts of barley sprouts were found to be the most effective. The 75% ethanol extracts of barley sprouts exhibited a strong reducing activity and ferric reducing antioxidant activity. As a result, the 75% ethanol extracts of barley sprouts showed stronger antioxidant activity than other extracts.

• Fisheries and Aquatic Sciences
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• v.25 no.6
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• pp.311-319
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• 2022

Hyaluronic acid from Liparis tessellatus eggs (HALTE) was grafted with caffeic acid (CA-g-HALTE), ferulic acid (FA-g-HALTE), gallic acid (GA-g-HALTE), and nisin (Nisin-g-HALTE) and investigated for their anti-inflammatory and antioxidant potential in lipopolysaccharides-stimulated RAW 264.7 mouse macrophages. Nitric oxide (NO) generation and prostaglandin E₂ activity were measured after treatment with the grafted HALTE samples. All grafted HALTE samples exhibited more antioxidant activity against 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radicals than 2,2-diphenyl-1-picrylhydrazyl radicals. Nisin-g-HALTE showed the least antioxidant activity. Additionally, the NO assay results showed that all grafted samples had no cytotoxic effect on RAW 264.7 macrophages and reduced macrophage activity after treatment. The most effective concentrations of CA-g-HALTE and FA-g-HALTE were found to be above 100 ㎍/mL. Increased sample concentration resulted in increased activity except with Nisin-gHALTE at 100 ㎍/mL. CA-g-HALTE, FA-g-HALTE, GA-g-HALTE, and Nisin-g-HALTE were found to have antioxidant and anti-inflammatory potential, which can be further explored for use in food, cosmetic, nutraceutical, and biomedical applications.

• Korean Journal of Clinical Laboratory Science
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• v.47 no.4
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• pp.194-202
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• 2015

Agaricus brasiliensis, belonging to the family Agaricaceae of Agaricales, Basidiomycota, has been used for edible and medicinal purposes. This study was initiated to evaluate the antioxidant, anti-diabetic, and nitric oxide inhibitory activities of fruiting bodies of A. brasiliensis extracted with methanol. The HPLC analysis of phenolic compounds from the mushroom extracts identified 10 phenolic compounds including gallic acid, procatechuic acid, chlorogenic acid, (-)-epicatechin, vanillin, rutin hydrate, naringin, quercetin, formononetin, and biochanin-A. The free radical scavenging activities of methanol extract were lower than that of positive control, BHT. The chelating effects of methanol extract were significantly higher than those of the positive control, BHT at the all concentrations tested. The methanol extract exhibited the lower reducing power activities compared with the positive control at the 0.5~6.0 mg/mL concentration. The mushroom extract inhibited the ${\alpha}$-glucosidase activity by 54.48% and 78.43% at the 1.0 and 2.0 mg/mL while acarbose, the positive control, inhibited the ${\alpha}$-glucosidase activity by 51.77% and 81.81% at the same concentrations, respectively. Nitric oxide (NO) production in lipopolysaccahride (LPS) induced RAW 264.7 cells were inhibited by the methanol extracts in a concentration dependent manner. Therefore, it is concluded that fruiting bodies of A. brasiliensis contained natural antioxidant, anti-diabetic, and anti-inflammatory substances which can be useful for human health.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.37 no.1
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• pp.61-66
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• 2011

Antioxidant activity of Geranium nepalense subsp. thunbergii extract was evaluated by DPPH free radical scavenging assay. Geranium nepalense subsp. Thunbergii extract contains tannin, (-)epicatechin, kaempferitin, kaepferol -7-rhamnoside, brevifolin, corilagin, pyrogallol, ellagitannin, geraniin, gallic acid, succinic acid, quercetin, protocatechuic acid, etc. Geranium nepalense subsp. Thunbergii showed excellent antioxidant activity compared to positive control, quercetin. Geranium nepalense subsp. thunbergii extract showed a 98.33 % inhibition of DPPH radical at a concentration of $50\;{\mu}g/mL$. Quercetin showed a 78.05 % inhibition of DPPH radical at the same concentration. To investigate reactive oxygen species (ROS) scavenging activity, Geranium nepalense subsp. thunbergii extract was treated to human keratinocytes (HaCaT). $IC_{50}$ value of Geranium nepalense subsp. thunbergii extract was $43.22\;{\mu}g/mL$ and $IC_{50}$ value of quercetin was $102.35\;{\mu}g/mL$. Geranium nepalense subsp. thunbergii extract showed excellent antioxidant activity. Skin irritation test and cytotoxicity test suggested that Geranium nepalense subsp. thunbergii extract is a safe antioxidant ingredient for cosmetics.

• Korean Journal of Food Science and Technology
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• v.45 no.3
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• pp.299-304
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• 2013

The major hydrophilic bioactive compounds (chlorogenic acid, caffeine, total phenolics, and flavonoids) and the antioxidant capacity in a single size were evaluated for regular coffee varieties commercially available in Korea. The content of total phenols (63.83-110.12 mg gallic acid equivalents) and flavonoids (35.27-69.27 mg catechin equivalents) were spectrophotometrically determined, and the content of chlorogenic acid (5.17-69.78 mg) and caffeine (74.38-146.32 mg) were determined using HPLC-UV. All varieties of regular coffee studied showed antioxidant potential (88.78-487.52 mg trolox equivalents/serving size), which was conferred by their concentrations of phenolic compounds, caffeine and chlorogenic acid. There were significant (p<0.01) correlations between the total phenolics ($r^2$=0.732) and flavonoids ($r^2$=0.8705) and the antioxidant capacity, suggesting that these components were likely a significant contributor to the antioxidant capacity of commercial regular coffee brews.