• 대한한방내과학회지
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• 제39권4호
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• pp.717-731
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• 2018

Objectives: The purpose of this research is to investigate clinical studies of essential tremor in China. Methods: We investigated clinical studies of Traditional Chinese Medicine therapies in traditional Chinese medical journals for essential tremor through the China National Knowledge Infrastructure (CNKI) database. In total, 15 clinical articles were analyzed. This study examined the authors; published years; types of study; treatment methods for the treatment and control groups; periods; outcome assessment; and results. Results: Nine articles used acupuncture, 6 articles used herbal therapy. The most commonly used acupoints were GV20, GB20, LR3, LI4, GB34, SP6, and EX-HN1. The most commonly used herbs were Paeoniae Radix Alba, Angelicae Gigantis Radix, Cnidii Rhizoma, Gastrodiae Rhizome and Uncariae Ramulus et Uncus. Total effective rate was 66.67-96.7%; the treatment group values were significantly higher than the control group values in all papers. Tremor score, HAMD, curative effect of TCM syndrome, and incidence of adverse effects were significantly more affected in the treatment group than in the control group. Conclusion: More active clinical studies are needed on essential tremor, and the results of this study can be used as a basis for future research.

• 한국수정란이식학회지
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• 제16권1호
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• pp.1-5
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• 2001

A total of 92 unfertilized human oocytes were treated with ethanol (EtOH), calcium ionophore A23187 (CI) or electric pulse (EP) for activating pronuclear formation and subsequent development. In Experiment 1, there was a significant (P=0.0001) treatment effect on the activation of unfertilized oocytes. No spontaneous activation was occurred in the control, but activation treatments induced PN formation with various efficacy. More unfertilized oocytes (UFOs) were activated after EtOH or EP treatment than after CI treatment. EP was as effective (63.6 %) as EtOH, but fragmentation was observed in 43% of UFOs activated by EP. Proportion of UFOs that formed presumptive haploid PN (2 PNs+1 PB or 1 PN +2 PBs) was 33.3, 0 and 28.6% after EtOH, CI and EP treatments, respectively. In Experiment 2, a significant (P=0.0362) effect of immature oocytes (IOs) status on activation was fecund. IOs at the GVBD-MI oocytes had higher potential to form PN than those at the GV stage or with abnormal morphology (25 vs. 77.8%). The results of this study clearly demonstrated that the treatment of 10% ethanol for 5 min effectively induced the activation of UFOs. IOs could form pronucleus with high efficacy by ethanol treatment, as long as they grew beyond the GVBD stage.

• 대한한의학회지
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• 제16권1호통권29호
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• pp.132-140
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• 1995

Object : This study is designed to evaluate the correlation between the data of DITI(Digital Infrared Thermographic Imaging)examination and the changes of clinical symptom after the therapy of acupuncture in the patients with herniated cervical intervertebral disc. Content : The conservative therapy with acupuncture was performed during $4{\sim}5$ weeks, The acupuncture points of SI3, B62, TE3, G34, LI4, S36, GV26, G21, SII5 were used. In the pre- and post therapy, DITI examinations were performed in patients who had HIVD and were treated by acupuncture simultaneously, and then tried to correlate the results of clinical symptoms with the difference of thermographic findings at pre-acupuncture and post-acupuncture. Setting : The standard routine thermoghaphic examinations were performed with thermography(DITI) in the 15 patients with lumbar disc herniations at pre- and post acupuncture. Patient : Thermographic imaging of 15 cases were analyzed. They has disc herniations in X-ray, CT scan and MRI and they were treated by acupuncture theraphy in our hospital from March, 1994 to January, 1995. Result : 1) The causes were trauma(60%), retention of phlegm and fluid(8.7%) and cold & moisture(33.3%) 2) Levels of herniated cervical disc are C2/3(1 case). C3/4(2 cases), C5/6 & C6/7 (2 cases), C6/7(4 cases), Normal(6 cases). 3) Classifications of thermographic pattern are radiculopathy(80%), spot(13.3%), and nonspecific(6.7%) in order. 4) In evaluation Results of clinical symptom are Excellent(80%), Good(6.7%), and Poor(6.7%). Data of DITI are Excellent(80%), Good(6.7%), Fair(6.7%), and Poor(6.7%). Conclusion ; Acupuncture showed good results over 86.7% in clinical evaluation and 86.7% in DITI. Thermographic examination was valuable in the evaluation of therapeutic effect of acupuncture treatment.

• 한국수정란이식학회지
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• 제33권1호
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• pp.1-11
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• 2018

Cellular cyclic adenosine-3' 5'-monophosphate (cAMP) modulator is known as meiotic inhibitor and can delays spontaneous maturation in IVM experiment. Among many cAMP modulators, the role of Pituitary adenylate cyclase activating polypeptide (PACAP) on IVM isn't known. The purpose of this study is to improve the maturation of oocytes derived from follicles ${\leq}3mm$ in diameter through PACAP as meiotic inhibitor during pre-in vitro maturation (pre-IVM). First, we checked PACAP and its receptors in cumulus cells and, to establish the optimal phase and concentration of PACAP for pre-IVM, we conducted chromatin configuration assessments. As a result, the rate of GV (Germinal Vesicle) according to duration of pre-IVM was significantly decreased 12 h and 18 h after IVM (87.1 and 84.1%, respectively) compared to 0 h (99.4%). When COC was cultured for 18 h, the GV rate in the $1{\mu}M$ of PACAP treatment group (82.1%) was significantly higher than any other PACAP treatment groups (60.5, 64.1, 74.4 and 69.9 %, respectively). So, we divided into four groups as follows; MF (the conventional IVM group, obtained from follicle from 3 to 6 mm in diameter), SF (the conventional IVM group, obtained from follicle ${\leq}3mm$ in diameter), Pre-SF(-)PACAP (IVM group including 18 h pre-IVM without $1{\mu}M$ of PACAP, obtained from follicle ${\leq}3mm$ in diameter) and Pre-SF(+)PACAP (IVM group including 18 h pre-IVM with $1{\mu}M$ of PACAP, obtained from follicle ${\leq}3mm$ in diameter). To examine the effect of PACAP during pre-IVM, we investigated analysis of nuclear maturation, intracellular glutathione (GSH) and reactive oxygen species (ROS) levels. In cumulus cells, PACAP receptors, ADCYAP1R1 and VIPR1 were detected but were not detected in oocytes. After IVM, the Pre-SF(+)PACAP had the highest Metaphase II rate (91.7%) among all groups (P<0.05). The GSH levels in the MF and Pre-SF(+)PACAP were significantly higher than in the other groups (P<0.05) and ROS levels was no significant difference among all groups. In conclusion, these results indicated that even though the oocytes were derived from SF, pre-IVM application of PACAP improved meiotic and cytoplasmic maturation by regulating intracellular oxidative stress.

• 한국가축번식학회지
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• 제26권2호
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• pp.105-110
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• 2002

본 연구는 소형견의 불임 해결과 번식효율 증진을 위해 소형견 난소 난포로부터 채취한 난자를 활성화 처리후 부고환 정자로 ICSI시켰을 때 체외발생율을 조사하기 위하여 수행하였다. 1. 난포란을 회수 후 24, 48시간 배양하였을 때 배양시간에 따른 GV, MI, MII로의 체외발생율은 각각 14/30(46.7%), 2/30(6.7%), 8/30(26.7%)였고 48시간 배양 시간에 따른 GV, MI, MII로의 체외발생율은 각각 l1/30(36.7%), 3/30(10.0%), 9/30(30.0%)였다. 2. 난포란을 회수 후 48시간 배양하였을 때 배양액에 따른 MII로의 체외발생율은 SOF액(10/30, 30.3%)에서의 배양이 TCM-199액(7/30, 23.3 %)보다 높은 체외발생율을 나타냈다. 3. 활성화 처리 난자에 부고환 정자로 ICSI를 하였을 때 상실배와 배반포로의 체외발생율은 각각 3/16(18.8%), 4/16(25.0%)로서 비활성화 처리 난자군의 3/13(23.1%), 1/13(7.7%)에 비해 높은 체외발생율을 나타냈다. 4. 활성화 처리 난자에 신선정자, 부고환 정자 및 동결 융해한 부고환 정자로 ICSI를 하였을 때 체외발생율은 각각 8/18(44.4%), 5/16(31.3%), 2/14(14.3%)로서 동결 부고환 정자 처리군은 신선정자 처리군에 비해 낮은 체외발생율을 나타냈다.

• 대한중풍순환신경학회지
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• 제8권1호
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• pp.28-33
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• 2007

Objectives and methods : The Euibujipsung is one of the huge-scale encyclopedias about Oriental Medicine. To search the most frequently used aupoints for dysarthria after stroke, we used Euibujipsung CD-ROM database with several chinese character keyword concerned with vernal function(語, 言, 音, 啞, 瘖, etc). Results : We found four popular acupoints(PC5, GV20, GV16, TE6), and five meridians (Governor vessel, Gall Bladder, Heart, Large Intestine and Triple Energizer). We also found that the extra meridians were used more frequently than other type of meridians. Conclusion : We think that these findings can give further ideas to clinical practice and research fields for stroke rehabilitation in Oriental medicine.

• Asian-Australasian Journal of Animal Sciences
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• 제21권4호
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• pp.532-537
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• 2008

In order to investigate the factors affecting the culture of mouse preantral follicles in vitro, we examined the effect of culture media, protein supplements, and culture period on their growth. The oocyte diameter (initial size: $55.6{\pm}2.5{\mu}m$) was progressively increased during culture, and the maximum size ($72.0{\pm}2.4{\mu}m$) was reached on day 10 of the in vitro culture. The chromatin configuration in the germinal vesicle (GV) oocyte progressively shifted from a non-surrounded nucleolus (NSN) to a surrounded nucleolus (SN). On day 10 of the culture, most of the oocytes progressed to the SN pattern. The survival and metaphase II rates of the oocytes in alpha-minimal essential medium (alpha-MEM) were significantly higher (p<0.05) than those in Waymouth and tissue culture medium (TCM)-199. As a protein source, fetal bovine serum (FBS) was more suitable for the culture of mouse preantral follicles as compared to human follicular fluid (hFF) and bovine serum albumin (BSA); the optimal concentration of FBS was 5%. These results suggest that in a culture of mouse preantral follicles, alpha-MEM and 5% FBS are an optimal medium and a protein source, respectively; further, the 10 days of culture is required for the complete growth of oocytes in this culture system.

• Journal of Acupuncture Research
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• 제19권3호
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• pp.168-179
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• 2002

Objective : To research the trend of the study related to electroacupuncture and to establish the hereafter direction for the study on electroacupuncture. Methods : We have referred to PubMed, with electroacupuncture (Limits: 5 Years, only items with abstracts, English, Human) Results : 1. We have searched 67 papers in 49 journals on electroacupuncture. 2. The pattern of study was as follows: review articles-8 papers in 8 journals, RCT articles-22 papers in 16 journals, meta-analysis-2 papers in 2 journals, and clinical trials-29 papers in 14 journals. 3. How frequently each Meridians was used for electroacupuncture in the searched papers was as follows: Large Intestine Meridian-14 times, Stomach Meridian-12 times, GallBladder Meridian-5 times, Governor Vessel-4 times, Conception Vessel-3 times, Pericardium Meridian-2 times, Triple Energizer Meridian-2 times, and Liver Meridian-2 times in that order. 4. The acupoints used in the searched papers were as follows: LI4-8 times, ST36, LI11, and GB34-3 times, GV20, LR3, and PC6-2 times in that order. 5. The diseases in the searched papers were as follows: pain, stroke, and others. Conclusion : More clinical data would be needed to prove the effects of electroacupuncture for better application.

• 대한한방체열의학회지
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• 제7권1호
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• pp.55-65
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• 2009

Purpose: This study was performed to evaluate DITI (Digital Infrared Thermographic Imaging)'s potential for diagnosis and indicator of abdominal cold hypersensitivity treatment from the clinical case study. Methods: The patient was a 35-year-old woman who was suffered by general coldness, sweating and general pain. The patient was treated by Herb medicine, Acupuncture treatment, Moxa treatment and Physical Treatment. The progress of symptoms was evaluated by checking the change of VAS, and inspecting the DITI. We studied the statistical significance from the difference of DITI results before and after treatment by paired T-test using SPSS windows (version 12.0). Results: The general coldness, sweating and general pain of this patient have declined, especially the abdominal coldness improvement showing. As a results, the temperature of four abdominal sites(GV4,6,12,17) after treatment were statistically higher than those before treatment. With comparison of Indang, DITI difference of abdominal sites before and after treatment were statistically significant from paired T-tests. Conclusion: The results suggest that DITI can be used for the diagnosis and assessment of abdominal cold hypersensitivity treatment.

• 대한약침학회지
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• 제22권4호
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• pp.279-283
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• 2019

Hominis placenta pharmacopuncture, a treatment that injects Hominis placenta extract into acupoints, has been suggested in the literature and researches that it could be used for cognitive decline. We experienced a case of mild neurocognitive disorder treated with Hominis placenta pharmacopuncture. Hominis placenta pharmacopuncture could be a possible treatment modality producing substantial clinical result in cognitive function which is assessed with Mini-Mental State Examination-Dementia Screening (MMSE-DS), Korean Version of Montreal Cognitive Assessment (MoCA-K), and Korean-Dementia Rating Scale (K-DRS). A 84-year-old man with mild neurocognitive disorder received Hominis placenta pharmacopuncture on GV20, CV12, and bilateral ST36 for a month. The results of neuropsychological examination showed increase in scores after treatment of Hominis placenta pharmacopuncture. Before treatment, they were 15 points for MoCA-K, and 120 points for K-DRS (7.6%), but after treatment, they elevated by 21 points for MoCA-K and 137 points for K-DRS (100%). MMSE-DS score was 28 points, unchanged before and after treatment. It did not cause any side-effect. Hominis placenta pharmacopuncture could be a safe option for treating mild neurocognitive disorder.