• Journal of Acupuncture Research
• /
• 제24권3호
• /
• pp.175-185
• /
• 2007

Objectives : The purpose of this study is to evaluate the clinical effect of Moxa-Pellet treatment for nasal symptoms and quality of life(QOL) in patients with allergic rhinitis. Methods: Subjects were voluntarily recruited through newspaper and internet advertisement. In this randomized, single blind and controlled study, experimental group (n=19) received real Moxa-Pellet treatment and control group(n=20) received sham Moxa-Pellet treatment for 3 weeks. Acupuncture points used were $LI_4$, $ST_{36}$, $LU_7$, $GV_{14}$ and $GB_{20}$. Patient's nasal symptoms and QOL were assessed before and after 3 weeks treatment by the Nasal Symptom Scores(NSS) and the Medical Outcomes Study 36-Item Short-Form Health Survey(SF-36). Results : The results were as follows; 1. Among items of NSS, Sneezing' Rhinorrhea' Itching scores and Total Nasal Symptom Scores(TNSS) were significantly improved in experimental group after 3 weeks Moxa- Pellet treatment(p<0.05). In control group, there was no significant difference in all items except Headache item of NSS. 2. Among 8 domains of SF- 36, experimental group showed significant difference in Role-Emotional(RE), Mental Health(MH) and Bodily Pain(Bp) after 3 weeks Moxa- Pellet treatment(p<0.05). Control group showed no significant difference in all domains except one domain(Role limitation-physical). Conclusions: These results suggest that Moxa-Pellet treatment can be applicable to improve nasal symptoms and QOL in the patients with allergic rhinitis. Further long tenn studies on the Moxa-Pellets treatment's sustaining power and safety is needed.

• 한국가축번식학회지
• /
• 제17권2호
• /
• pp.85-92
• /
• 1993

In the previous study, we observed that Purine has a time dependent effect in maintaining the oocytes in meiotic arrest, and human fetal cord serum(HFCS) and human mature follicular fluid(HMFF) reverse the GVBD suppressed by purines. And it was reported that purine has a harmful effect on the development of oocytes or embryos, when they were cultured for a long time, in vitro. Therefore this study was performed to investigate the effects of purine on extrusion rates of 1st pb and viability of oocytes cultured for a long time, in vitro. Immature oocytes(GV stage) were collected from ovaries of 25~28 day old ICR mice at 48 hrs after PMSG injection. Cumulus-enclosed and denuded oocytes collected were assigned randomly to one of several culture conditions. Some of the oocytes were cultured in 4mM hypoxanthine for 24hr, and the extrusion rates of 1st pb and viability of the oocytes were assessed at every 12 hrs. In the viability, the oocytes showed granulation, pigmentation of cytoplasm or lysis of 1st pb extruded were regarded as degenerating oocytes. Also some of the oocytes were cultured in hypoxanthine for 12 hrs then the resulting oocytes were transferred to hypoxanthine-free medium and cultured for 12 hrs to determine whether the inhibitory effect of hypoxanthine on the 1st pb extrusion was reversible. The rest of the oocytes were cultured in medium containing hypoxanthine and adenosine for 18 hrs to compare the 1st pb extrusion be attendant upon hte concentration of HFCS or HMFF supplemented. Hypoxanthine suppressed the extrusion of 1st pb and viability of the oocytes significantly, when they were cultured for more than 12 hrs and the harmful effect of hypoxanthine was showed in denuded oocytes, prominently. The suppressive effect of hypoxanthine was reversed by just removal of the hypoxanthine from the cultrue medium. Also there was no difference in reverse the pb extrusion rate suppressed between HFCS and HMFF. The extrusion rate of 1st pb in medium containing adenosine and hypoxanthine was increased in line with the concentration of HFCS or HMFF supplemented. Hypoxanthine suppressed the extrusion of 1st pb and viability of the oocytes significantly, when they were cultured for more than 12 hrs and the harmful effect of hypoxanthine was showed in denuded oocytes, prominetly. The suppressive effect of hypoxanthine was reversed by just removal of the hypoxanthine fromthe culture medium. Also there was no difference in reverse the pb extrusion rate suppressed between HFCS and HMFF. The extrusion rate of 1st pb in medium containing adenosine and hypoxanthine was increased in line with the concentration of HFCS or HMFF supplemented.

• Reproductive and Developmental Biology
• /
• 제29권2호
• /
• pp.127-131
• /
• 2005

The objective of this study was to examine the effect of EGF on meiotic maturation and pronuclear (PN) formation of porcine oocytes. Prepubertal gilt cumulus-oocyte-complexes (COCs) aspirated from $2\~6mm$ follicles of abbatoir ovaries were matured in TCM199 containing 0.1mg/ml cysteine, $0.5{\mu}/ml$ FSH and LH, and EGF (0, 5, 10, 20, 40 ng/ml) for 22 hr at $39^{\circ}C$ in a humidified atmosphere of $5\%$ $CO_2$ in air. They were then cultured for an additional 22hr without hormones. In Experiment 1, to examine the nuclear maturation at 44hr of culture, the expanded cumulus cells were removed by vortexing for 1 min in 3 mg/ml hyaluronidase. The oocytes were fixed in acetic acid: methanol (1:3, v/v) at least for 48 hr and stained with $1\%$ orcein solution for 5 min. Nuclear status was classified as germinal vesicle (GV), germinal vesicle breakdown (GVBD), prophase-metaphase I (PI-MI), and PII-MII under microscope. In Experiment 2, to investigate PN formation, oocytes were fertilized with Percoll-treated freshly ejaculated sperm $(1\times10^5\; cells/ml)$ in mTBM with $0.3\%$ BSA and 2mM caffeine for 5hr, and cultured in NCSU-23 medium with $0.4\%$ BSA. At 6hr of culture, the embryos were fixed in $3.7\%$ formaldehyde for 48hr and stained with 10ug/ml propidium iodide for 30 min. PN status was classified as no or one PN (unfertilized), 2 PN (normal fertilized) and $\geq3$ PN (polyspermy). Differences between groups were analyzed using one-way ANOVA after arc-sine transformation of the proportional data. The rate of oocytes that had reached to PII-MII were significantly (P<0.05) higher in all groups added EGF than that of non-treated group $(67\%)$, but it did not differ among the all added groups $(86\%,\;85\%,\;79\%\;and\;81\%$, in 5, 10, 20 and 40 ng/ml EGF, respectively). No differences on the incidence of 2PN were observed in all treated groups $(25\%,\;30\%,\;33\%,\;29\%\;and\;29\%$, in 0, 5, 10, 20 and 40 ng/ml EGF, respectively), however, in non-treated group, polyspermy tended to be increased ($66\%\;vs\;. 58\%,\;54\%,\;52\%\;and\;55\%$, 0 vs. 5, 10, 20, 40 ng/ml EGF, respectively). These results suggest that EGF can be effectively used as an additive for enhancing oocyte maturation and reducing the incidence of polyspermy in pig.

• Clinical and Experimental Reproductive Medicine
• /
• 제32권4호
• /
• pp.331-336
• /
• 2005

Objective: To investigate the effects of female age on in vitro maturation and fertilization of immature oocytes from controlled ovarian hyperstimulation (COH) in human IVF-ET program. Method: A total of 96 immature oocytes (GV & metaphase I) obtained from 40 cycles of IVF-ET (29 patients). The mean age of female patients was $31.8{\pm}3.1years$. Ovulation was triggered by urinary or recombinant hCG. Immature oocytes were cultured with YS medium containing 30% of patients' human follicular fluids, LH (1 IU/mL), FSH (1 IU/mL) and EGF (10 ng/mL), and then matured oocytes were fertilized by ICSI. In vitro maturation and fertilization of immature oocytes were analyzed according to age of female (< 34 or ${\geq}34years$). Results: The maturation rate was similar between two groups (68% vs 64%). The fertilization rate of in?vitro-matured oocytes was higher in patients < 34 years old, but there was no statistical significance (64% vs 50%, p=0.347). The fertilization rate of in-vitro-matured oocytes was significantly lower compared with those of in-vivo-matured oocytes in both age groups (64% vs 79%, p=0.035, 50% vs 86%, p=0.007). Conclusion: In older female group, fertilization rate of in-vitro-matured oocytes seems to be decreased. Further investigations should be warranted to increase fertilization potential of in-vitro-matured oocytes.

• Journal of Acupuncture Research
• /
• 제21권5호
• /
• pp.123-136
• /
• 2004

Objective : Because the cause and etiology of chronic headache is not yet fully explained, the treatment of this symptom is not simple. This study compares the effects of aroma acupuncture and normal acupuncture applied on chronic headache patients, in order to establish a primary data for further studies of new treatments and developments of new practical acupunctures. Methods : 38 clinical experiment participants were gathered and through a questionnaire patients who experienced headache for more than 4 hours a day and more than 15 days per month were qualified as Chronic Headache patients. The qualified patients were classified in to two groups, aroma acupuncture group(Aroma AT group, n=23) and normal acupuncture group(AT group, n=15). Treatment was applied 2 times a weeks for 8 weeks. The acupoints, GV20, HN23, ST8, HN46, TE17, GB20, LI20, LI11, LI14, ST36, and LR3 were stimulated for 20 minutes in both the groups. The effects of both groups were analyzed using VAS scores and BPI (Brief Pain Inventory). Results : The VAS pain scores improved significantly in both groups. The decrease rate was larger in the Aroma AT group(p<0.05). The BPI scores for the both groups also decreased. The improvement in the Aroma AT group was larger(p<0.05). Conclusion : Aroma acupuncture applied on chronic headache patients showed effects of relieving pain, improving general activities, changing the mood, and improving sleeping behaviors. These effects were analyzed using VAS scores and BPI. The Aroma AT group showed significant changes of VAS and BPI compared to the AT group, suggesting that this new practical acupuncture is very effective when treating chronic headaches. Further researches are needed to evaluate the distinct functional mechanism of aroma acupuncture, but this study is meaningful in the sense that it will act as a fundamental study to build on.

• Journal of Acupuncture Research
• /
• 제29권5호
• /
• pp.109-118
• /
• 2012

Objectives : The aim of this study was to evaluate the effectiveness and safety of acupuncture for chronic fatigue syndrome and idiopathic chronic fatigue. Methods : A randomized, controlled pilot trial was conducted. Twenty-four participants were randomized into one of the two groups: an acupuncture group(n=12) and wait-list group(n=12). The treatment group received 12 sessions of acupuncture over 4 weeks. Eight points($GV_{20}$; bilateral GB20, $BL_{11}$, $BL_{13}$, $BL_{15}$, $BL_{18}$, $BL_{20}$, and $BL_{23}$) were selected for needling. The control group did not receive acupuncture treatment during study period and follow-up were done in the $5^{th}$ and $9^{th}$ weeks after randomization in both groups. The primary outcome was fatigue severity scale(FSS) and the secondary outcomes included a short form of stress response inventory(SRI-short form), beck depression inventory(BDI), and insomnia severity index(ISI). Safety was assessed at every visit. Results : There were statistically significant differences in the between group values of FSS at $5^{th}$ weeks after randomization(p=0.0002), SRI-short form, BDI, and ISI at $5^{th}$, $9^{th}$ weeks after randomization(p<0.01). There were no adverse events. Conclusions : The results suggest that acupuncture is associated with benefits on the short-term outcomes in chronic fatigue syndrome and idiopathic chronic fatigue.

• Journal of Acupuncture Research
• /
• 제24권5호
• /
• pp.105-111
• /
• 2007

Objectives : The electroacupuncture(EA) was often used in Oriental medicine clinic and basic researches. However, there have been neither any national standard of acupuncture needles in Korea nor many studies about the property of acupuncture needles for applying electrical stimulations. Therefore we investigated the electrical corrosion property of acupuncture needles used for EA stimulation in clinic. Methods : We observed the occurrence of electrical corrosion like changes in the surface of acupuncture needles after EA stimulation with various parameters(electrical stimulation intensity, treatment duration, diameter of needle) under a light microscope. We performed EA stimulation on Baekhoe($GV_{20}$) or Joksamli($ST_{36}$) of rats with intensity of 0.025, 0.05, 0.075, 0.1, 0.25 or 0.5mA at a 10Hz frequency. Results : The electrical corrosion-like changes were found on the surface of needles when the EA stimulation was given over 0.05mA of intensity. When electrical stimulation was treated for 10min, the electrical corrosion-like changes were low, but the electrical corrosion-like changes gradually increased by the treatment duration(20, 30min). We also found that acupuncture needles of 0.2mm diameter were produced more extensive electrical corrosion-like changes than acupuncture needles of 0.3 or 0.4mm diameter by electrical stimulation. Conclusions: These results suggested that EA stimulation could produce electrical corrosion-like changes of acupuncture needles through intensity of current, duration of electric stimulation and/or diameter of needle.

• 한방재활의학과학회지
• /
• 제23권4호
• /
• pp.59-71
• /
• 2013

Objectives The aim of this review is to figure out which acupoints are frequently used to treat low back pain and which exercises are effective to low back pain. Methods We searched the three electronic database (RISS, KISS, KTKP) and manually checked related Korean journals and reference lists up to June 2013. We investigated the frequency of acupoints for using treatment of low back pain. We Introduce exercise therapies we can do easily at home without professional skills for treatment low back pain. Results We included 72 articles in this study. The most frequently adopted acupoints were BL25, BL23, BL24, GB30, BL40, BL26, BL60, GB34, BL52, BL57, GB39. The most frequently adopted meridian pathways were BL, GB, GV, ST. The most frequently adopted disease causing low back pain were HIVD (Herniated intervertebral disc), Lumbar sprain, Spinal stenosis, Compression fracture, Cauda equina syndrome, Spondylolisthesis, Ankylosing spondylitis. There are many exercise therapies (Lumbar stabilizing exercise, Resistance exercise, Bareunmom physical exercise, Mckenzie exercise, Williams exercise, Emblass exercise, Swiss ball exercise, Thera band exercise, Yoga) for treatment low back pain. Conclusions These results suggest that most frequently adopted acupoints were foot taeyang urinary bladder channel acupoints. Beacuse foot taeyang urinary bladder channel is passes through the waist, it will be effective for treatment of low back pain. Also exercise therapy is effective for enhance Muscular strength. In order to treatment of low back pain, Acupuncture treatment is also important, as well as exercise therapy.

• 대한한방내과학회지
• /
• 제39권2호
• /
• pp.165-175
• /
• 2018

Purpose: To report on a patient with subarachnoid hemorrhage and deep vein thrombosis treated with a combination of Korean and Western medicine. Methods: A patient diagnosed with subarachnoid hemorrhage and deep vein thrombosis was treated with herbal medicine (加減淸熱瀉濕湯 (Gagam-cheongyeolsaseub-tang)), acupuncture (GV2, GB20, LI11, LI4, TE5, GB34, ST36, SP9, ST38, GB39, SP6, LR3), moxibustion (CV6, CV9), and Western medicine for deep vein thrombosis and subarachnoid hemorrhage. To evaluate edema, one of the classic symptoms of deep vein thrombosis, we measured leg circumference at a specified time. Results: After taking Gagam-cheongyeolsaseub-tang and being treated with acupuncture, moxibustion, and Western medicine, the patient showed improvement in leg edema. The left calf circumference decreased from 37.5 cm to 34.5 cm. Conclusions: Korean medicine, acupuncture, moxibustion, and Western medicine may provide effective treatment for deep vein thrombosis in patients with subarachnoid hemorrhage, but further study is needed.

• 한국축산시설환경학회지
• /
• 제13권3호
• /
• pp.195-200
• /
• 2007

무창 육성 비육돈사에 대한 악취 제어 효율 비교분석을 위하여 Biofilter 덕트에 의한 악취 제어 연구를 수행하였다. 본 실험은 서울대 부속목장 실험돈사에서 실시하였으며 실험결과는 다음과 같다. 1. 무창 육성비육돈사에서 여름철 최대 배기시 바이오필터 외부로 배출되는 공기속도는 볏짚(.0.77 m/s), ALC(0.56 m/s)으로 측정되었다. 2. 실험시작 5일 후 가스검지기(GV-100)에 의한 간이측정시 암모니아($NH_3$) 농도는 필터 통과 후 볏짚($2mg/{\ell}$), ALC(3ppm)로 측정되어 부자재 사이의 공극율이 높은 볏짚이 높게 나타났다. 부자재에 따른 필터외부에서의 먼지측정 결과 볏짚($93\;mg/m^3$), ALC($32\;mg/m^3$)으로 측정되어 볏짚은 볏짚 간의 넓은 공극에서 발생하는 먼지 및 돈사내부의 먼지가 혼합되어 필터 외부로 배출되어 돈사내부보다 높게 측정되었다. 3. 황화수소($H_{2}S$) 가스농도 변화는 돈사내부에서는 측정일 7일(6 ppm), 21일(5 ppm) 및 36일(7 ppm) 경과함에 따라 어느 일정 시점에서 황화수소 가스농도는 증가하지 않았으나 다른 필터여재에서는 일정한 경향은 보이지 않았다. 이상의 실험결과를 종합해 볼 때 무창 육성비육 돈사에서 Biofilter를 이용한 악취저감 효과를 확인할 수 있었다.