• 한국수산과학회지
• /
• 제42권6호
• /
• pp.633-641
• /
• 2009

Maturity and spawning of the atka mackerel, Pleurogrammus azonus, was investigated based on the samples collected by gill net and set net in the East Sea from January 2006 to March 2009. Gonadosomatic index (GSI) began to increase in October, and reached a maximum between November and December. After spawning GSI began to decrease from January. The spawning period was from October to January, and main spawning period was from November to December. Annual reproductive cycles of this species could be divided into five successive stages in females; early growing stage (June), late growing stage (July to August), mature stage (September to October), ripe and spent stage (November to January), and recovery and resting stage (January to May). Males showed four successive stages: growing stage (June to August), mature stage (September to October), ripe and spent stage (October to December), and recovery and resting stage (January to May). P. azonus could be one of polycyclic species spawning two times or more during one spawning season. Number of total and mature eggs in the fecundity were proportional to fork length. The fork length at 50% group maturity was estimated to be 26.9 cm.

• 한국양식학회지
• /
• 제20권3호
• /
• pp.154-159
• /
• 2007

Manchurian trout (Brachymystax lenok) is an endangered fish species in East Asia including the Korean peninsula. To establish a method for artificial propagation and to improve our understanding of the reproduction in the species, we have produced recombinant gonadotropins, follicle-stimulating hormone (r-mtFSH) and luteinizing hormone (r-mtLH), which may play central roles in reproductive activities. In the present study, the biological activities of the recombinant hormones were analyzed by gonadosomatic index (GSI), ovarian follicle diameter, and sex steroid levels in mature rainbow trout (Oncorhynchus mykiss). In the 6th day post-injection, FSH-injected fish were slightly decreased in the GSI value, although there were no significant differences among those of control, r-mtFSH, and r-mtLH treatments. Injection of the r-mtFSH increased follicle diameters significantly as compared with those of control- and r-mtLH-injected fish. The plasma steroid levels showed wide differences in the groups at 1, 3, or 6th day post-injection. Despite the variable steroid levels, three individuals receiving either r-mtFSH or r-mtLH showed a great increase in a maturation-inducing steroid, $17{\alpha},20{beta}$-dihydroxy-4-pregnen-3-one, at 3 and 6 days. Taken together, these results suggest that biological efficacies of the recombinant FSH and LH should be further studied in the Manchurian trout.

• 한국어류학회지
• /
• 제23권4호
• /
• pp.300-304
• /
• 2011

2008년 10월에서 2009년 9월까지 통영 주변해역에서 출현하는 별망둑 (Chaenogobius gulosus) 333개체를 대상으로 산란기, 성비, 군성숙체장, 포란수를 조사하였다. 별망둑의 체장(standard length, SL)은 2.0~12.6 cm의 범위를 보였다. 별망둑 암컷 생식소중량지수 (GSI)의 월변동을 살펴보면 2월에 연중 가장 높은 값을 보였으며, 그 이후 4월까지 감소하는 양상을 나타내었다. 간중량지수 (HSI)는 1월에 4.58로 가장 높은 값을 나타내었으며, 산란성기인 2월과 3월에 각각 3.14와 3.42로 낮은 값을 보였다. 따라서 별망둑의 산란기는 12월에서 4월로 판단되었다. 별망둑의 월별 성비는 1 : 1.06으로 암.수간 유의한 차이를 나타내지 않았다(${\chi}^2$-test, p>0.05). 별망둑 암컷의 50% 군성숙체장은 7.9 cm로 나타났으며, 9.0 cm 이상에서는 모든 개체가 성숙하였다. 포란수는 1,112~6,059개의 범위를 보였으며, 평균 3,011개를 나타내었다.

• 화약ㆍ발파
• /
• 제24권1호
• /
• pp.21-28
• /
• 2006

현재 가장 많이 사용되고 있는 암반분류법인 RMR 이나 Q 분류법을 이용하여 조사단계에서 암반평가를 할 때, 평가요소의 하나인 RQD 값을 구하기 위한 시추작업이 제한적으로 이루어지고 있고, 또한 시공단계에서도 시추작업은 거의 이루어지지 않고 있는 실정이다. 실제 현장조사에서는 RQD값은 일반적으로 유추되거나 간접적인 방법을 통해서 이루어지고 있는 실정이다. 또한 암반내의 절리간격조사도 여러 군의 절리가 존재할 경우 그룹별 간격의 측정이 용이하지 않으며 불연속면의 연속성 등 불연속면의 특성에 관한 측정이 쉽지 않다는 것이다. 절리간격 요소도 설제로는 RQD와 중복되는 요소로서 시추 코아에 의존하지 않고 보다 쉽게 암반평가를 실시할 수 있는 새로운 암반분류법의 개발이 필요하다. 이를 위해서 요구되는 요소들을 측정하지 않고도 암반의 구조적인 형태와 절리의 거칠기와 변형정도로 표시되는 불연속면의 표면적인 조건만을 관찰함으로써 암반평가를 실시할 수 있는 방법인 GSI 의 요소들을 RMR 방법과 결합하여 새로운 암반분류법을 제시하고자 하는 것이다.

• 한국수산과학회지
• /
• 제53권1호
• /
• pp.19-26
• /
• 2020

The maturity and spawning of pacific sand eels Ammodytes japonicus, was investigated using samples collected by gill net in coastal waters near Donghae, Gangwon-do, from January 2017 to December 2018. Monthly changes were analyzed in maturity stage, gonadosomatic index (GSI), egg diameter, fecundity (F), and total length (TL) at 50% group maturity. The average TLs of female and male A. japonicus were 18.5 and 18.1 cm, respectively. The spawning period was from November to March, with the peak from December to February based on monthly changes in GSI and maturity stages. The egg diameter during the spawning-capable stage was 300-1000 (main mode 500-600) ㎛. Fecundity ranged from 6,411 eggs at 16.7 cm fork length to 45,771 eggs at 24.7 cm fork length. The relationship between TL and F was F=0.00005TL^4.2715 (R²=0.7216), such that F increased with TL. TL at 50% group maturity was estimated to be 15.1 cm for both sexes.

• 한국발생생물학회지:발생과생식
• /
• 제22권2호
• /
• pp.175-182
• /
• 2018

In order to examine the effects of four different light spectra (white, red, green, and blue) on the oocyte maturation, the change of reproductive parameters, via brain-pituitary-gonad (BPG) axis in grass puffer, were investigated. After exposure four different light spectra for 7 weeks, the abundance of gonadotropin-releasing hormone (GnRH) mRNA which is a type of seabream (sbGnRH) and two different subunit of gonadotropin hormones mRNAs, follicle-stimulating hormone ($fsh{\beta}$) mRNA and luteinizing hormone ($lh{\beta}$) mRNA, were analyzed in the brain and pituitary. Histological analysis showed that the mature oocyte ratio in the green spectrum was higher than other light spectra-exposed groups. Gonadosomatic index (GSI) and oocyte developmental stage were also investigated in the gonad based on histological observations. GSI value with the presence of yolk stage oocytes was significantly increased in the green spectrum-exposed group when compared to that of the other light-exposed groups (white, red, and blue) (p<0.05). The abundances of sbGnRH mRNA and $fsh{\beta}$ mRNA in the green spectrum-exposed group were also significant higher than those of the other light spectra-exposed groups (p<0.05). These results indicate that the maturation of oocyte in grass puffer can be accelerated by exposure to the spectrum of green. To better understand the molecular mechanism for the maturation of oocyte in grass puffer, further study examining the relationship between oocyte development and its related genes is required.

• Fisheries and Aquatic Sciences
• /
• 제13권4호
• /
• pp.332-339
• /
• 2010

Maturity and spawning of the snailfish, Liparis ochotensis was investigated based on samples collected by gill net in the East Sea of Korea from December 2008 to December 2009. The average total length of L. ochotensis was 63.4 cm and 64.1 cm for females and males, respectively. Gonadosomatic index (GSI) began to increase in July, reaching a maximum in November, then began to decrease in December. Monthly changes in the GSI values of L. ochotensis were similar for females and males. Annual reproductive cycle of this species can be divided into five successive stages in females: early growing stage (May), late growing stage (June to July), mature stage (August to September), ripe and spent stage (September to December), and recovery and resting stage (January to April). Males show four successive stages; growing stage (May to July), mature stage (August to September), ripe and spent stage (September to December), and recovery and resting stage (January to April). Relationship between total length (TL) and fecundity (F) was F=$0.00003TL^{4.002}$ ($R^2$ = 0.703), and F increased with TL. Total length at 50% group maturity was estimated to be 55.8 cm. Our findings suggest that the spawning period takes place from September to December, with the main spawning period occurring from October to December.

• Fisheries and Aquatic Sciences
• /
• 제17권1호
• /
• pp.115-121
• /
• 2014

Eyestalk ablation (ESA) is commonly used in aquaculture to stimulate ovarian maturation in crustaceans, and methyl farnesoate (MF) affects crustacean molting and reproduction. To investigate the physiological effects of ESA and MF treatments on the shrimp Litopenaeus vannamei, we compared the effects of single eyestalk removal and MF injections. The ESA group had the lowest survival rate (50%), and individuals in the $0.1{\mu}g$ and $1.0{\mu}g$ MF-treated groups had survival rates of 80 and 73.3%, respectively. Conversely, molting numbers were highest in the ESA group, and similar to those of the 1.0-${\mu}g$ MF group. To investigate shrimp growth, we measured body weight during the experimental period and found that individuals in the ESA and $1.0{\mu}g$ MF groups showed significant increases in body weight. Furthermore, to investigate the effects of ESA and MF treatments on gonadal maturation, the gonad somatic index (GSI) was calculated after the experiment. All treated groups (ESA and MF) had higher GSI values than the control group, but the ESA and $1.0{\mu}g$ MF groups were not significantly different. Using histological ovary analysis, we determined that all treated groups showed indications of the previtellogenic stage, unlike the control group (immature stage). These results suggest that the high-MF-concentration treatment produced effects similar to those of ESA with respect to molting number, growth, and ovarian maturation.

• 수산해양교육연구
• /
• 제24권6호
• /
• pp.1003-1012
• /
• 2012

The gonadosomatic index (GSI), sex steroid hormones and gonadal development of roughscale sole Clidoderma asperrimum cultured in indoor tank were investigated to evaluate its sexual maturation and reproductive cycle. The highest GSI values of female and male were $6.91{\pm}4.03$ (May) and $0.16{\pm}0.08$ (August), respectively. The reproductive cycle would be classified into four successive developmental stages: growing stage (December to February), maturation stage (March to April), ripe and spawning stage (May to June), recovery and resting stage (July to November). The highest plasma testosterone (T) and estradiol-$17{\beta}$ ($E_2$) levels of female were $259.4{\pm}76.8$ and $633.3{\pm}182.5$ pg/mL, respectively in May. Also $17{\alpha}$, $20{\beta}$-dihydroxy-4-pregen-3-one ($17{\alpha}$, $20{\beta}$-OHP) levels of female peaked in April before spawning season ($244.2{\pm}42.5$ pg/mL). The highest plasma testosterone (T) and 11-ketotestosterone levels of male were $231.0{\pm}46.0$ and $273.9{\pm}54.5$ pg/mL, respectively in April. But there was no significant difference in $17{\alpha}$, $20{\beta}$-OHP.

• 한국어류학회지
• /
• 제21권2호
• /
• pp.93-99
• /
• 2009

본 연구는 전남바다목장 해역 내에 서식하는 감성돔의 성숙과 산란에 관한 연구로, 2007년 3월부터 2008년 2월까지 낚시어업에 의해 어획된 시료를 대상으로 수행하였다. 감성돔의 50% 성전환 체장은 26.7 cm로 추정되었고, 암컷의 생식소 발달과정 및 생식소중량지수(GSI) 월변화로부터 감성돔의 산란기는 3~7월, 주 산란기는 5~6월로 추정되었다. 포란수는 최소 39,596립(체장 32.2 cm)에서 최대 2,894,913립(체장 42.0 cm)으로 계수되었고, 체장(FL)과 포란수(F)간의 관계식은 $F=1.264FL^{3.869}(R^2=0.736)$으로, 일반적 경골어류에서와 같이 체장이 커질수록 포란수도 증가하는 경향을 보였다. 산란에 참여하는 50% 군성숙도 체장은 암컷은 25.8 cm, 수컷은 19.0 cm이다.