Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
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Effects of Manchurian Trout Gonadotropins on Sexual Maturation in Female Rainbow Trout

  • Park, Woo-Dong (Division of Marine Molecular Biotechnology, Faculty of Marine Bioscience and Technology, Kangnung National University) ;
  • Ko, Hye-Yeon (Division of Marine Molecular Biotechnology, Faculty of Marine Bioscience and Technology, Kangnung National University) ;
  • Kim, Dae-Jung (Aquaculture Research Team, Headquarters for Aquaculture, National Fisheries Research and Development Institute (NFRDI)) ;
  • Lee, Cheul-Ho (Yeongdong Inland Fisheries Research Institute, NFRDI, National Fisheries Research and Development Institute (NFRDI)) ;
  • Sohn, Young-Chang (Division of Marine Molecular Biotechnology, Faculty of Marine Bioscience and Technology, Kangnung National University)
  • Published : 2007.08.31
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Abstract

Manchurian trout (Brachymystax lenok) is an endangered fish species in East Asia including the Korean peninsula. To establish a method for artificial propagation and to improve our understanding of the reproduction in the species, we have produced recombinant gonadotropins, follicle-stimulating hormone (r-mtFSH) and luteinizing hormone (r-mtLH), which may play central roles in reproductive activities. In the present study, the biological activities of the recombinant hormones were analyzed by gonadosomatic index (GSI), ovarian follicle diameter, and sex steroid levels in mature rainbow trout (Oncorhynchus mykiss). In the 6th day post-injection, FSH-injected fish were slightly decreased in the GSI value, although there were no significant differences among those of control, r-mtFSH, and r-mtLH treatments. Injection of the r-mtFSH increased follicle diameters significantly as compared with those of control- and r-mtLH-injected fish. The plasma steroid levels showed wide differences in the groups at 1, 3, or 6th day post-injection. Despite the variable steroid levels, three individuals receiving either r-mtFSH or r-mtLH showed a great increase in a maturation-inducing steroid, $17{\alpha},20{beta}$-dihydroxy-4-pregnen-3-one, at 3 and 6 days. Taken together, these results suggest that biological efficacies of the recombinant FSH and LH should be further studied in the Manchurian trout.

Keywords

References

  1. Aida, K., T. Kato and M. Awaji, 1984. Effects of castration on the smoltification of precocious male masu salmon, Oncorhynchus masou. Nippon Suisan Gakkaishi, 50, 565-571 https://doi.org/10.2331/suisan.50.565
  2. Choi, E., H. Ko, J. Shin, M-A. Kim and Y. C. Sohn, 2005. Expression of gonadotropin genes in Manchurian trout Brachymystax lenok and production of recombinant gonadotropins. Fish. Sci., 71, 1193-1200 https://doi.org/10.1111/j.1444-2906.2005.01083.x
  3. Chyung, M. K., 1977. The fishes of Korea. Il Ji Sa Publishing Co., Seoul, Korean, pp 122
  4. Kim, D. J., E. O. Kim, M. W. Park, Y. C. Cho and S. G. Lim, 2006. Plasma sex steroid hormone profiles in artificially maturing wild eel, Anguilla japonica. J. Aquaculture, 19, 267-274
  5. Klein, J., L. Lobel, S. Pollak, B. Lustbader, R. T. Ogden, M. V. Sauer and J.W. Lustbader, 2003. Development and characterization of a long-acting recombinant hFSH agonist. Human Reprod., 18, 50-56 https://doi.org/10.1093/humrep/deg024
  6. Ko, H., W.D. Park, D.J. Kim, M. Kobayashi and Y.C. Sohn, 2007. Biological activities of recombinant Manchurian trout FSH and LH: their receptor specificity, steroidogenic and vitellogenic potencies. J. Mol. Endocrinol., 38, 99-111 https://doi.org/10.1677/jme.1.02163
  7. Kobayashi, M., K. Aida and L. Hanyu, 1988. Hormone changes during ovulatory cycle in goldfish. Gen. Comp. Endocrinol., 69, 301-307 https://doi.org/10.1016/0016-6480(88)90018-4
  8. Kobayashi, M., T. Morita, K. Ikeguchi, G. Yoshizaki, T. Suzuki and S. Watabe, 2006. In vivo biological activity of recombinant goldfish gonadotropins produced by baculovirus in silkworm larvae. Aquaculture, 256, 433-442 https://doi.org/10.1016/j.aquaculture.2006.01.016
  9. Nagahama, Y., M. Yoshikuni, M. Yamashita, N. Sakai and M. Tanaka, 1993. Molecular endocrinology of oocyte growth and maturation in fish. Fish Physiol. Biochem., 11, 3-14 https://doi.org/10.1007/BF00004545
  10. Okada, T., L. Kawazoe, S. Kimura, Y. Sasamoto, K. Aida and H. Kawauchi, 1994. Purification and characterization of gonadotropin I and II from pituitary glands of tuna (Thunnus obesus). Int. J. Peptide Protein Res., 43, 69-80 https://doi.org/10.1111/j.1399-3011.1994.tb00377.x
  11. Pankhurst, N. W. and M. J. R. Porter, 2003. Cold and dark or warm and light: variations on the theme of environmental control of reproduction. Fish Physiol. Biochem., 28, 385-389
  12. Patino, R., 1997. Manipulations of the reproductive system of fishes by means of exogenous chemicals. Progressive Fish- Culturist, 59, 118-128 https://doi.org/10.1577/1548-8640(1997)059<0118:MOTRSO>2.3.CO;2
  13. Planas, J. V., J. Athos, F. W. Goetz and P. Swanson, 2000. Regulation of ovarian steroidogenesis in vitro by follicle-stimulating hormone and luteinizing hormone during sexual maturation in salmonid fish. Biol. Reprod., 62, 1262-1269 https://doi.org/10.1095/biolreprod62.5.1262
  14. Reshetnikov, Y. S., N. G. Bogutskaya, E. D. Vasil'eva, E. A. Dorofeeva, A. M. Naseka, O. A. Popova, K. A. Savvaitova, V. G. Sideleva and L. I. Sokolov 1997. An annotated check-list of the freshwater fishes of Russia. J. Ichthyol., 37, 687-736
  15. Sullivan, C. V., N. Hiramatsu, A. M. Kennedy, R. W. Clark, G. M. Weber, T. Matsubara and A. Hara, 2003. Induce maturation and spawning: opportunities and applications for research on oogenesis. Fish Physiol. Biochem., 28, 481-486 https://doi.org/10.1023/B:FISH.0000030635.92568.0a
  16. Suzuki, K., H. Kawauchi and Y. Nagahama, 1988a. Isolation and characterization of subunits of two distinct gonadotropins from chum salmon pituitary glands. Gen. Comp. Endocrinol., 71, 302-306 https://doi.org/10.1016/0016-6480(88)90258-4
  17. Suzuki, K., Y. Nagahama and H. Kawauchi, 1988b. Steroidogenic activities of two distinct salmon gonadotropins. Gen. Comp. Endocrinol., 71, 452-458 https://doi.org/10.1016/0016-6480(88)90274-2
  18. Swanson, P., J. T. Dickey and B. Campbell, 2003. Biochemistry and physiology of fish gonadotropins. Fish Physiol. Biochem., 28, 53-59 https://doi.org/10.1023/B:FISH.0000030476.73360.07
  19. Tanaka, H., H. Kagawa, K. Okuzawa and K. Hirose, 1993. Purification of gonadotropins (pmGTH I and II) from red seabream (Pagrus major) and development of homologous radioimmunoassay for pmGTH II. Fish Physiol. Biochem., 10, 409-418 https://doi.org/10.1007/BF00004507
  20. Van Der Kraak, G., K. Suzuki, R. E. Peter, H. Itoh and H. Kawauchi, 1992. Properties of carp gonadotropin I and gonadotropin II. Gen. Comp. Endocrinol., 85, 217-229 https://doi.org/10.1016/0016-6480(92)90005-5
  21. Yaron, Z., G. Gur, P. Melamed, H. Rosenfeld, A. Elizur and B. Levavi-Sivan, 2003. Regulation of fish gonadotropins. Int. Rev. Cytol., 225, 131-185 https://doi.org/10.1016/S0074-7696(05)25004-0
  22. Zohar, Y. and C. C. Mylonas, 2001. Endocrine manipulations of spawning in cultured fish: from hormones to genes. Aquaculture, 197, 99-136 https://doi.org/10.1016/S0044-8486(01)00584-1