• Mycobiology
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• v.48 no.3
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• pp.195-203
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• 2020

Marine yeasts have tremendous potential in industrial applications but have received less attention than terrestrial yeasts and marine filamentous fungi. In this study, we have screened marine yeasts for amylolytic activity and identified an amylase-producing strain PH-Gra1 isolated from sea algae. PH-Gra1 formed as a coral-red colony on yeast-peptone-dextrose (YPD) agar; the maximum radial growth was observed at 22 ℃, pH 6.5 without addition of NaCl to the media. Based on the morphology and phylogenetic analyses derived from sequences of internal transcribed spacer (ITS) and a D1/D2 domain of large subunit of ribosomal DNA, PH-Gra1 was designated Sporidiobolus pararoseus. S. pararoseus is frequently isolated from marine environments and known to produce lipids, carotenoids, and several enzymes. However, its amylolytic activity, particularly the optimum conditions for enzyme activity and stability, has not been previously characterized in detail. The extracellular crude enzyme of PH-Gra1 displayed its maximum amylolytic activity at 55 ℃, pH 6.5, and 0%-3.0% (w/v) NaCl under the tested conditions, and the activity increased with time over the 180-min incubation period. In addition, the crude enzyme hydrolyzed potato starch more actively than corn and wheat starch, and was stable at temperatures ranging from 15 ℃ to 45 ℃ for 2 h. This report provides a basis for additional studies of marine yeasts that will facilitate industrial applications.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2004.08a
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• pp.139-145
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• 2004

It is well known that human errors is involved in most of maritime accidents. For the purpose of reducing the influence of human elements on maritime activities, it is necessary to identify the human unsafe acts in those activities. The commonly used methods in identification of human unsafe acts are maritime accident statistics or case analysis. With the statistics data, people could roughly identify what kinds of unsafe acts or human errors have played active role in the accident, however, they often neglected some active unsafe acts while overestimated some mini-unsafe acts because of the inherent shortcoming of the methods. There should be some more accurate approaches for human error identification in maritime accidents. In this paper, the application of technique called grey relational analysis (GRA) into the identification of human unsafe acts is presented. GRA is used to examine the extent of connections between two digits by applying the, methodology of departing and scattering measurement to actual distance measurement. Based on the statistics data of maritime accidents occurred in Chinese waters in last 10years, the relationship between the happening times of maritime accidents and that of unsafe acts are established with GRA. In accordance with the value of grey relational grade, the identified main human unsafe acts involved in maritime accidents are ranked in following orders: improper lookout, improper use of radar and equivalent equipment, error of judgment, act not in time, improper communication, improper shiphandling, use of unsafe speed, violating the rule and ignorance of good seamanship. The result shows that GRA is an effective and practical technique in improving the accuracy of human unsafe acts identification.

• Journal of the Korea Convergence Society
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• v.10 no.11
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• pp.149-159
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• 2019

Six sigma is an innovative management movement which provides improved business process by adapting the paradigm and the trend of market and customers. Suitable selection of six sigma project could highly reduce the costs, improve the quality, and enhance the customer satisfaction. There are existing studies on the selection of Six Sigma projects, but few studies have been conducted to select the correct project under an incomplete information environment. The purpose of this study is to propose the application of integrated MCDM techniques for correct project selection under incomplete information. The project selection process of six sigma involves four steps as follows: 1) determination of project selection criteria 2) calculation of relative importance of team member's competencies 3) assessment with project preference scale 4) finalization of ranking the projects. This study proposes the combination methods by applying group fuzzy Analytical Hierarchy Process (AHP), an easy defuzzified number of Trapezoidal Fuzzy Number (TrFN) and Grey Relational Analysis (GRA). Both of the weight of project selection criteria and the relative importance of team member's competencies can be evaluated by group fuzzy AHP. Project preferences are assessed by easy defuzzified scale of TrFN in case of incomplete information.)

• Journal of Korean Society of Industrial and Systems Engineering
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• v.38 no.2
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• pp.82-90
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• 2015

Location selection of distribution centers is a crucial task for logistics operators and key decision makers of an organization. This is a multi-criteria decision making (MCDM) process which includes both quantitative and qualitative criteria. In order to propose an optimized location selection model, this research suggests a hierarchical group of evaluation criteria : 5 major criteria with 15 sub-criteria. The MCDM approach presented in this research, by integrating Grey Relational Analysis (GRA) with Analytic Hierarchy Process (AHP), tends to rectify the overall quality and uncertainty of the values of evaluation criteria. An example of a location selection case in Korea is illustrated in this study to show the effectiveness of this method.

• Archives of Pharmacal Research
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• v.24 no.3
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• pp.214-218
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• 2001

The mechanisms responsible for the antidiabetic activity of both the white ginseng radix (Ginseng Radix Alba, GRA) and the rootlet (Cinseng Radix Palva, GRP) were investigated. After a four week oral administration, the fasting blood glucose levels in the GRA- and GRP-treated groups were lower when compared to the control group. To elucidate the hypoglycemic mechanism(s) of the ginseng radices, glucose absorption from the small intestine, hepatic hexokinase and glucose-6-phosphatase activities, in addition to PPAR-${\gamma}$ expression in adipose tissue were examined. The results strongly suggest that GRA can improve hyperglycemia in KKAy mice, possibly by blocking intestinal glucose absorption and inhibiting hepatic glucose-6-phosphatase, and GRP through the upregulation of adipocytic PPAR-$\gamma$ protein expression as well as inhibiting intestinal glucose absorption.

• Journal of Navigation and Port Research
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• v.44 no.4
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• pp.291-297
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• 2020

In recent years, an increase in deaths and injuries of port cargo handling workers, has raised the need for more effective accident management. The purpose of this study was to evaluate the accident risk for port cargo handling workers and assess ports with high accident risk within the Korean alternative ports using the Entropy & GRA (Grey Relational Analysis). To achieve this purpose, first, 11 Korean ports were selected and the evaluative factors for their outranking evaluation by brainstorming were extracted. Second, the Grey Relational Coefficient of 11 alternative ports was calculated using the GRA. This paper, finally, determined the priority orders of accident risk through calculation of the Grey Relational Grade as the link Grey Relational Coefficient method and the weights of the evaluative factors were calculated by using the Entropy method. In the proposed model, eight criteria such as cargo worker, old cargo worker, work hours, facilities environment, steel cargo volumes, cargo volumes, injury numbers, and death numbers were collected. Busan port was identified as highest accident risk port, and so it should be a top priority to develop a plan to mitigate the risk.

• Journal of the Korean Institute of Gas
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• v.17 no.1
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• pp.56-66
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• 2013

The gas production plants supply the inert gas to production plants for maintaining safe operation and also supply combustible, flammable, explosive and toxic gases as functions of basic materials needed for producing chemical goods. In addition, gas plants need to be safe and reliable operation because they are operated under high temperature, high pressure, cryogenic and catalytic reactions. As these plants have a complex process in operation, there has been a risk that major industrial accidents such as a fire, explosion and toxic gas released, also risks of asphyxiations by inert gases and burns caused by high temperature and cryogenic substances. This study is to carry out the semi quantitative risk assesment method which is the generic risk analysis (GRA). This method is applied to air separation unit(ASU) to identify its initial risk, safety barriers, residual risk and elements important for safety(EIS). The result of this study, suggested the management tools and procedures of implementation for EIS management.

• Journal of Nutrition and Health
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• v.27 no.8
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• pp.805-818
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• 1994

This study investigated the effect of enteral nutrition supplementation on glucose and lipid metabolism in non-insulin dependent diabetes mellitus(NIDDM) patients(n=29). Nutrition formula(400kcal/day) were supplied daily for eight weeks as a substitute for a snack or a meal. Subjects were divided into three groups based on changes of fasting blood glucose(FBG), glucose response area(GRA) on oral glucose tolerance test(OGTT), before and after intake of nutrition formula : group 1(the group of a decrease in FBG and GRA, n=20), group 2(the group of a decrease in FBG and an increase in GRA, n=4), and group 3(the group of an increase in FBF and GRA, n=5). Before nutrition supplementation, group 3 showed a longer tendency of DM duration and a lower tendency of insulin and C-peptide response are than those of group 1 and 2. At 8 weeks after nutrition supplementation, group 1 showed a significant increase in insulin and C-peptide response areas but group 2 and 3 showed no change in those areas. After nutrition supplementation, all three groups showed a tendency of decrease in glycated hemoglobin and no significant changes in the levels of serum triglycerides, HDL, LDL, total cholesterol, albumin, transferrin, creatinine, GOT and GPT. The results suggest that using an enteral nutrition formula in NIDDM patients is a good substitute for a meal or snack and could improve blood glucose control without any changes in lipid levels, and liver and kidney functions. The beneficial effect of nutrition supplementation on glycemic control resulted from components of nutrition formula had such as additional fiber and high monounsaturated fatty acid as the source of fat to be helpful 세 glycemic control in diabetics.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.3
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• pp.2178-2184
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• 2015

With the development of wireless network technologies, mobile users may use various networks easily and expect more advanced services. On the other hand, it may bring on some problems with network resource management that should lead the service provider to improve the current service quality and manage the network resource efficiently. This paper proposes the optimized radio resource management (RRM) scheme that integrates the Grey Relational Analysis (GRA) and game theory. The first applies the GRA to determine the Grey Relation Coefficient (GRC) factors that represent the network preference, and the network provider then selects the requested service that provide maximum payoff through Nash Equilibrium. Six requested services that have one application service among four different types of service classes were considered and the game was played repeatedly. In WiMAX, WLAN 1 and WLAN 2 game, the maximum payoff of each players was 93, 90.6 and 92.8 respectively. The experimental results show that every requested service can be selected by the network provider. Consequently, the proposed radio resource management mechanism is more effective in heterogeneous wireless networks.

• Parasites, Hosts and Diseases
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• v.50 no.3
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• pp.233-238
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• 2012

The precise diagnosis of the acute toxoplasmosis in pregnant women and immunocompromsied patients has critical importance. Most of the commercially available assays use the whole Toxoplasma soluble extract as the antigen. However, the assays currently available for the detection of specific anti-Toxoplasma antibodies may vary in their abilities to detect serum immunoglobulins, due to the lack of a purified standardized antigen. The aim of this study was production and evaluation of the usefulness of the recombinant Toxoplasma gondii GRA7 antigen for the serodiagnosis of Toxoplasma gondii IgM and IgG by ELISA. A total of 70 T. gondii IgM positive sera, 74 T. gondii IgG positive sera, and 60 sera from subjects who were not infected with T. gondii were examined. These sera were shown different absorbance values in ELISA test. To control the specificity of the rGRA7 other parasitic diseases, for example, echinococcosis, malaria, leishmaniasis, fascioliasis, and strongyloidiasis were tested of which none showed positive results. Sensitivity and specificity of the generated recombinant IgG ELISA in comparison with commercial ELISA (com ELISA) were 89% and 90%, and the sensitivity and specificity of the generated recombinant IgM ELISA were 96% and 90%, respectively. The results obtained here show that this antigen is useful for diagnostic purposes.