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Studies on Microflora of the Paddy and Upland Soils of Korea -I. Distribution of Microflora of the Paddy Soils (우리나라 논, 밭토양의 미생물상(微生物相)에 한 연구 -I. 논토양의 미생물(微生物) 분포조사(分布調査))

  • Yoo, Ick-Dong;Yun, Seh-Young;Lee, Myong-Goo;Rhu, Jin-Chang
    • Korean Journal of Soil Science and Fertilizer
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    • v.16 no.2
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    • pp.195-202
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    • 1983
  • Sixty paddy soil samples were collected from the different Korean agricultural climatic area to find relationship between soil physicochemical properties and soil microorganisms. The results are summarized as follows : 1. The mean numbers of microorganisms in collected paddy soils were $121.8{\times}10^5$ in bacteria (B), $22.5{\times}10^5$ in actinomycetes (A) and $32.4{\times}10^3$ in fungi (F) per gr soil. The ratios of B/F, B/A, and A/F were 385, 5.1 and 82, respectively. 2. Number of soil microorganism was lowest in Mountainous area, lower in Eastern Coast, Gyeonggi Bay and Chungcheong Continental area, but higher in Honam Plain and Southern Part. 3. The significant positive correlation were obtained between the number of microorganisms and soil chemical properties, available phosphorous, $K^+$, $Mg^{++}$, T-C and soil pH. 4. The number of soil microorganism was in the order of Clay loamy soil > Clayey soil > Loamy soil > Sandy loamy soil.

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Effects of Artificial Acid Rain on Chemical Properties of Korean Forest Soils (인공산성우(人工酸性雨)가 삼림토양(森林土壤)의 화학적(化學的) 성질(性質)에 미치는 영향(影響))

  • Joo, Yeong Teuk;Kim, Young Chai
    • Journal of Korean Society of Forest Science
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    • v.83 no.3
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    • pp.280-285
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    • 1994
  • This study was conducted to investigate the effects of acid deposition on forest soil, major five Korean forest soils(Brown, Dark red, Gray brown, Red and Yellow, and Volcanic ash forest soils) The samples were subjected to receive 1200mm($100mm{\times}12$ times) of artificial acid rain adjusted to pH5.6, 4.0, 3.0 and 2.0. The results obtained of major importance are summarized as follow ; 1. Ca appeared mostly affected at pH treatment of 2.0, while less affected by other pH treatments. Leaching of Ca rapidly increased with increasing of artificial acid rain acidity and application times in Dark red forest soil. 2. In the cases of Mg, K and Na, they showed gradual increase with the addition of artificial acid rain. Mg and Na losses showed similar leaching patterns, but they didn't show difference among the five forest soils. 3. Exchangeable canon concentrations in the soil leachates, which looked slightly different among the five forest soils, were the highest in pH2.0 treatment. Hydrogen ion comsumption capability by exchangeable canon was the highest in Dark red forest soil followed by Volcanic ash, Red and Yellow, Gray brown and Brown forest soils when artificial acid rain were treated.

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Eliminatory Effect of Mixture including Hot Water Extract of Dendropanax morbifera Lev. on Alcohol-induced Blood Alcohol Concentration and Hangover in Rat (흰쥐에서 황칠나무 열수 추출물을 포함한 혼합물의 혈중 알코올 농도와 숙취 해소 효과)

  • Na, Ju-ryun;Kim, Eun;Park, Soyi;Lee, Kihoon;Jeong, Eui-seon;Kim, Jinseok;Kim, Youngjae;Kim, Sunoh
    • Journal of Chitin and Chitosan
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    • v.23 no.4
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    • pp.267-276
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    • 2018
  • This study was performed to investigate the ameliorating effect of a hangover beverage mixture (SBJ) that contains Dendropanax morbifera Lev. and several medicinal plant extracts, on hepatoprotection and alcohol-metabolizing enzymes in alcohol-induced hangover in both in vitro and in vivo models. In human hepatoma cell line, HepG2, 300 mM of ethanol-induced hepatotoxicity was significantly improved by pretreatment of SBJ by dose-dependent manner. In the in vivo study, administration of alcohol to rats raised to the concentration of blood alcohol and lactate dehydrogenase (LDH). Blood alcohol and LDH levels in SBJ-treated rats significantly decreased at 0.5 h and 8 h after acute ethanol administration (40%, 4.6 g/kg body weight) as compared to alcohol-treated rats. Hepatic alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) activity were significantly higher in SBJ-treated rats than in alcohol-treated rats. SBJ supplementation reduced formation of malondialdehyde (MDA), and inhibited reductions of hepatic superoxide dismutase (SOD), hepatic glutathione (GSH), glutathione-S-transferase (GST), glutathione reductase (GR) and glutathione peroxidase (GPx) levels, compared with rats administered alcohol. Plasma catalase (CAT), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels showed unaltered resulted in all experimental groups compared with the control group. These results suggest that SBJ exhibit hepatoprotective properties by enhancing ADH, ALDH activity and stimulating the antioxidant defense system in alcohol-induced hangover.

HPV 16 E6/E7 Transgenic Mice have Hyperkeratosis and Elevated ROS Related Enzyme Activities

  • Kim, Myoung-Ok;Lee, Eun-Ju;Kim, Sung-Hyun;Park, Jun-Hong;Kyoungin-Cho;Jung, Boo-Kyung;Kim, Hee-Chul;Sol ha Hwang;Kim, Sun-Jung
    • Proceedings of the KSAR Conference
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    • 2003.06a
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    • pp.45-45
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    • 2003
  • Human papillomavirus type 16(HPV16) has been known to the major factor for the development of uterine cervical carcinomas. We have extended these studies to investigate the in vivo activities of HPV-16 E6/E7 when expressed in squamous epithelia of transgenic mice. Grossly, hK14HPV16E6/E7 transgenic mice had multiple phenotypes, including wrinkled skin that was apparent prior to the appearance of hair on neonates, thickened ears, and loss of hair in adults. In the transgenic mice, the wrinkled skin phenotype on the body and legs died at the age of 3∼4 weeks. Histological analysis of demonstrated that E6/E7 causes epidermal hyperplasia in multiple transgenic lineages with high penetrance. This epithelial hyperplasia was characterized by an expansion of the proliferating compartment and an expansion of the keratinocyte and was associated with hyperkeratosis. These transgenic mice expressed E6/E7 transgene mainly in skin, heart, pancreas and kidney. Hyperplasia was found at the skin. The enzyme activities of GR, GPx and CuZnSOD were measured from the transgene cause keratinocyte at the skin. The specific enzyme activities were significantly higher in transgenic mice skin compared to the normal mice skin. Thus these transgenic mice may be useful for the develpment of antioxidant enzymes or other therapies for HPV-associated hyperkeratosis.

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Effect of Gupoongjeseuptang (GPJST) on DNCB (dinitrochlorobenzene)-induced Atopic Dermatitis-like Model NC/Nga Mice (구풍제습탕(驅風除濕湯)이 DNCB로 유도된 NC/Nga mice의 아토피 피부염에 미치는 영향)

  • Yoon, Jae-Eun;Kim, Yun-Hee;Han, Jae-Kyung;Kim, Yun-Hee
    • The Journal of Pediatrics of Korean Medicine
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    • v.22 no.3
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    • pp.105-137
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    • 2008
  • Objectives : The purpose of this study is to investigate the effect of Gupoongjeseuptang (GPJST) on atopic dermatitis by in vivo experiment using NC/Nga atopic dermatitis mouse, which has histological and clinical similarities to the atopic dermatitis of human. Methods : To investigate the effect of GPJST on atopic dermatifis, we evaluated atopic dermatitis-like skin lesions by clinical skin index and analyzed immunological parameters in peripheral blood mononuclear cells (PBMCs), splenocytes, draining lymph node (DLN) and performed skin histology in ears and dorsal skin of atopic dermatitis-like skin NC/Nga mouse in vivo. Results : In vivo, clinical skin severity score were significantly lower in GPJST group than control group. IgE, IL-6, $TNF-{\alpha}$, IgG1, IgM, IgG2a and IgG2b levels in serum decreased remarkably in GPJST group than control group. Also, total absolute number of $CD3^+CD69^+$, and $CCR3^+$ cells recovered as normal in PBMCs and $CD3^+$, $CD3^+CD69^+$ decreased significantly compared with control group in isolated DLN from NC/Nga mouse and total absolute number of $CD11b^+Gr-1^+$, $CCR3^+CD3^+$ in dorsal skin of NC/Nga mouse decreased by GPJST. We analyzed ear and neck-back skin after biopsy and dyeing by hematoxyline/eosin (H&E) and toluidine staining (mast cells marker) and obtained results that GPJST are very effective to histological symptoms (dermal and epidermal thickening, hyperkeratosis and inflammatory cell (CD4, $CCR3^+$) infiltration). Conclusions : This study demonstrates immunological activity of GPJST on atopic dermatitis-like model mice.

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Optimization of membrane fouling process for mustard tuber wastewater treatment in an anoxic-oxic biofilm-membrane bioreactor

  • Chai, Hongxiang;Li, Liang;Wei, Yinghua;Zhou, Jian;Kang, Wei;Shao, Zhiyu;He, Qiang
    • Environmental Engineering Research
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    • v.21 no.2
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    • pp.196-202
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    • 2016
  • Membrane bioreactor (MBR) technology has previously been used by water industry to treat high salinity wastewater. In this study, an anoxic-oxic biofilm-membrane bioreactor (AOB-MBR) system has been developed to treat mustard tuber wastewater of 10% salinity (calculated as NaCl). To figure out the effects of operating conditions of the AOB-MBR on membrane fouling rate ($K_V$), response surface methodology was used to evaluate the interaction effect of the three key operational parameters, namely time interval for pump (t), aeration intensity ($U_{Gr}$) and transmembrane pressure (TMP). The optimal condition for lowest membrane fouling rate ($K_V$) was obtained: time interval was 4.0 min, aeration intensity was $14.6 m^3/(m^2{\cdot}h)$ and transmembrane pressure was 19.0 kPa. And under this condition, the treatment efficiency with different influent loads, i.e. 1.0, 1.9 and $3.3kgCODm^{-3}d^{-1}$ was researched. When the reactor influent load was less than $1.9kgCODm^{-3}d^{-1}$, the effluent could meet the third discharge standard of "Integrated Wastewater Discharge Standard". This study suggests that the model fitted by response surface methodology can predict accurately membrane fouling rate within the specified design space. And it is feasible to apply the AOB-MBR in the pickled mustard tuber factory, achieving satisfying effluent quality.

Effect of Cr content on the FAC of pipe material at 150℃ (150℃에서 원전 2차측 배관재료의 Cr함량에 따른 유체가속부식 특성)

  • Park, Tae Jun;Kim, Hong Pyo
    • Corrosion Science and Technology
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    • v.12 no.6
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    • pp.274-279
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    • 2013
  • Flow accelerated corrosion (FAC) of the carbon steel piping in nuclear power plants (NPPs) has been major issue in nuclear industry. During the FAC, a protective oxide layer on carbon steel dissolves into flowing water leading to a thinning of the oxide layer and accelerating corrosion of base material. As a result, severe failures may occur in the piping and equipment of NPPs. Effect of alloying elements on FAC of pipe materials was studied with rotating cylinder FAC test facility at $150^{\circ}C$ and at flow velocity of 4m/s. The facility is equipped with on line monitoring of pH, conductivity, dissolved oxygen(DO) and temperature. Test solution was the demineralized water, and DO concentration was less than 1 ppb. Surface appearance of A 106 Gr. B which is used widely in secondary pipe in NPPs showed orange peel appearance, typical appearance of FAC. The materials with Cr content higher than 0.17wt.% showed pit. The pit is thought to early degradation mode of FAC. The corrosion product within the pit was enriched with Cr, Mo, Cu, Ni and S. But S was not detected in SA336 F22V with 2.25wt.% Cr. The enrichment of Cr and Mo seemed to be related with low, solubility of Cr and Mo compared to Fe. Measured FAC rate was compared with Ducreaux's relationship and showed slightly lower FAC rate than Ducreaux's relationship.

Study on the Hydrogen Treatment Effect of Vacuum deposited Pentacene Thin Film Transistors

  • Lee, Joo-Won;Chang, Jae-Won;Kim, Hoon;Kim, Kwang-Ho;Kim, Jai-Kyeong;Kim, Young-Chul;Lee, Yun-Hi;Jang, Jin;Ju, Byeong-Kwon
    • 한국정보디스플레이학회:학술대회논문집
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    • 2003.07a
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    • pp.668-672
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    • 2003
  • In order to reach the high electrical quality of organic thin film transistors (OTFTs) such as high mobility and on-off current ratio, it is strongly desirable to study the enhancement of electrical properties in OTFTs. Here, we report the novel method of hydrogen $(H_{2})$ plasma treatment to improve electrical properties in inverted staggered OTFTs based on pentacene as active layer. To certify the effect of this method, we compared the electrical properties of normal device as a reference with those of device using the novel method. In result, the normal device as a reference making no use of this method exhibited a field effect mobility of 0.055 $cm^{2}/Vs$, on/off current ratio of $10^{3}$, threshold voltage of -4.5 V, and subthreshold slope of 7.6 V/dec. While the device using the novel method exhibited a field effect mobility of 0.174 $cm^{2}/Vs$, on/off current ratio of $10^{6}$. threshold voltage of -0.5 V, and subthreshold slope of 1.49 V/dec. According to these results, we have found the electrical performances in inverted staggered pentacene TFT owing to this novel method are remarkably enhanced. So, this method plays a key role in highly improving the electric performance of OTFTs. Moreover, this method is the first time yet reported for any OTFTs

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γ-Aminobutyric acid (GABA) confers chromium stress tolerance in mustard (Brassica juncea L.) seedlings by modulating the antioxidant defense and glyoxalase systems

  • Al Mahmud, Jubayer;Hasanuzzaman, Mirza;Nahar, Kamrun;Rahman, Anisur;Hossain, Md. Shahadat;Fujita, Masayuki
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.235-235
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    • 2017
  • Chromium (Cr) toxicity is hazardous to the seed germination, growth, and development of plants. ${\gamma}$-Aminobutyric acid (GABA) is a non-protein amino acid and is involved in stress tolerance in plants. To investigate the effects of GABA in alleviating Cr toxicity, we treated eight-d-old mustard (Brassica juncea L.) seedlings with Cr (0.15 mM and 0.3 mM $K_2CrO_4$, 5 days) alone and in combination with GABA ($125{\mu}M$) in a semi-hydroponic medium. The roots and shoots of the seedlings accumulated Cr in a dose-dependent manner, which led to an increase in oxidative damage [lipid peroxidation; hydrogen peroxide ($H_2O_2$) content; superoxide ($O{_2}^{{\cdot}-}$) generation; lipoxygenase (LOX) activity], MG content, and disrupted antioxidant defense and glyoxalase systems. Chromium stress also reduced growth, leaf relative water content (RWC), and chlorophyll (chl) content but increased phytochelatin (PC) and proline (Pro) content. Furthermore, supplementing the Cr-treated seedlings with GABA reduced Cr uptake and upregulated the non-enzymatic antioxidants (ascorbate, AsA; glutathione, GSH) and the activities of the enzymatic antioxidants including ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), glutathione reductase (GR), glutathione peroxidase (GPX), superoxide dismutase (SOD), catalase (CAT), glyoxalase I (Gly I), and glyoxalase II (Gly II), and finally reduced oxidative damage. Adding GABA also increased leaf RWC and chl content, decreased Pro and PC content, and restored plant growth. These findings shed light on the effect of GABA in improving the physiological mechanisms of mustard seedlings in response to Cr stress.

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Evaluation of Growth, Carcass, Immune Response and Stress Parameters in Naked Neck Chicken and Their Normal Siblings under Tropical Winter and Summer Temperatures

  • Rajkumar, U.;Reddy, M.R.;Rao, S.V. Rama;Radhika, K.;Shanmugam, M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.4
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    • pp.509-516
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    • 2011
  • The performance of naked neck and normal chicken was evaluated with respect to growth, carcass, immune, biochemical and stress parameters under winter and summer seasons to assess the suitability of naked neck birds under high temperatures in the global scenario of climate change. The growth performance was significantly ($p{\leq}0.05$) higher in naked neck chicken in the summer season. The dressing percentage was significantly ($p{\leq}0.05$) higher in naked neck birds in both winter and summer season because of reduced plumage. The thigh, giblet and feather proportion significantly ($p{\leq}0.05$) varied between naked neck and normal chickens in summer season. The humeral immune response to sheep red blood cells (SRBC), Newcastle disease vaccine (NDV) and cutaneous basophil hypersensitivity (CBH) did not show any significant differences among the chicken groups. The protein and cholesterol concentration observed was within the normal ranges. The total cholesterol levels in plasma were significantly ($p{\leq}0.05$) lower in naked neck birds in both the seasons. H:L ratio was significantly ($p{\leq}0.05$) lower in summer season indicating less stress in naked neck chicken. Basophil and eosinophil concentration was significantly ($p{\leq}0.05$) higher in normal chicken in summer. The lipid peroxidation was higher in full feathered birds under summer stress. The enzyme glutathione reductase (GR) levels were significantly higher during the summer and varied significantly ($p{\leq}0.05$) between the normal and naked neck chicken in both seasons. The results indicated that the naked neck birds performed significantly better at high ambient temperatures with respect to growth, carcass and biochemical parameters. It was concluded that the ability of the naked neck chicken to adapt to high temperatures foresees a viable option for the biological mitigation of climate change.