• 대한의생명과학회지
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• 제6권3호
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• pp.187-192
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• 2000

구기자의 한방 약리효과를 보기 위하여 구기자 혼합 생체시료와 세포배양한 캘러스로부터 추출한 열수 추출물을 사염화탄소로 유발된 간장해 흰쥐 (SD계)에 하루 0.5 g/kg을 경구 투여한 결과, 대조군 (GOT 949, GPT 640 karmen unit)에 비하여 혼합 생체시료 (GOT 760.4, GPT 540 karmen unit)와 세포배양 캘러스 (COT 772.1, GPT 556.4 karmen unit) 처리구에서 거의 비슷한 혈청 중 GOT, GPT 활성의 상승을 억제하는 효과를 보였으며, 또한 비교약물 sylimarin 0.1 g/kg 투여군에서도 대조군에 비하여 혼합 생체시료 (GOT 492.6, GPT 320.4 karmen unit)와 세포배양 캘러스 (GOT 492.6, GPT 320.4 karmen unit) 처리구에서 거의 비슷한 혈청 중 GOT, GPT 활성의 상승을 억제하는 효과를 보였다. 이상의 결과를 통하여 볼 때 구기자 생체시료와 세포배양 캘러스는 간손상 흰쥐에 대한 간보호 효과가 동일하게 있는 것으로 판단된다.

• 생약학회지
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• 제23권4호
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• pp.268-275
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• 1992

We studied to screen medicinal plants having hepatoprotective activity with the primary cultured rat hepatocytes intoxicated with carbon tetrachloride cytotoxicity. The lowest concentration and treatment time of carbon tetrachloride giving the greatest intoxication to the primary cultured hepatocytes were observed in 10mM and 60 minutes, respectively. GTP and GOT activity of culture broth of the primary cultured rat hepatocytes intoxicated by $CCl_4$ cytotoxicity at this condition were increased 135.9% and 178.3% compared with that of the primaries cultured hepatocytes not treated with $CCl_4$, respectively. This increased GPT activity was inhibited by glycyrrizin, which was known to have hepatoprotective activity, and the inhibition activity was dependent on the concentration of glycyrrhizin. Forty species among the extracts obtained from 117 species of medicinal plants were shown to have the hepatoprotective activity. Among these 40 species, Prunus persica, Scutellaria baicalensis, Astragalus membranaceus, Tribulus terrestris, Caragana chamlagu, Acanthopanax sessiliflorum and Achyranthes japonica were indicated a lower GPT activity than that of Glycyrrhiza uralensis containing glycyrrhizin and GPT activity of these were indicated 75.5%, 70.0%, 59.0%, 77.5%, 60.0%, 75.0% and 79.0%, respectively.

• Journal of Ginseng Research
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• 제4권1호
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• pp.31-39
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• 1980

This study was prepared to observe some enzyme activities in the liver of mice treated with extracts of Ginseng anticancer compound, separated from the petroleum ether extracts by silicic acid chromatography, has the cytotoxic activity against cancer cells. Swiss mice, 72 heads were used (or this experiment and they were divdied into control, test group I and test group If, that test group I was injected crude extract and test group II was injected anticancer compound, while the control group was injected 0.9% NaCl solution. The injections were carried out 1,2,4 and 8 times once a day for 1-8 day, respectively. The liver was removed carefully from the mice at 24 hours after drugs injected, and homogenized at 4$^{\circ}C$ for enzyme study. The activities of glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase(GPT) were determined by Reitmen and Frankel method and lactic acid dehydrogenase activity was determined by Wroblewski methods in vitro. The results obtained are summarized as follows; 1. The GOT activity was increased 26%, crude extract and 16%, anticancer compound than those of control at 1st injected groups and decreased gradually according to increase of injection time, at 8th injected groups, the GOT activity was decresed by 16%, crude extract and 12%, anticancer compound. 2. The GPT activity was not changed significantly at 1st and 2nd injected groups, but, at 4th injected groups, the GPT activity was decreased 20%, crude extract and 14%, anticancer compound. While the GPT activity was recovered to normal value at 8th injected groups. 3. At 1st injected groups, the LDH activity was increased 17%, anticancer compound, while those of crude extract was shown normal value. At 2nd injected groups, the LDH activity increased 35yo:, crude extract while those of anticancer compound was showed normal value. And the LOH activity was recovered gradually at 4th and 8th injected groups.

• 생약학회지
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• 제26권1호
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• pp.51-56
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• 1995

To devise an in vitro screening method for antihepatotoxic activity, $CCl_4-induced$ cytotoxicities in primary cultures rat hepatocytes were examined. When rat hepatocytes were intoxicated with 0.5, 1.0 or 1.5 mM $CCl_4$ for 1.5, 3 or 19hr, in order of LDH>GOT>GPT release form hepatocytes was increased in a dose-dependent manner. Treatment with 1.5 mM $CCl_4$ for 1.5 hr showed maximum increase in activity of LDH, GOT or GPT released in the medium compared with the control. At this experimental condition, well known antihepatotoxic substances, glycyrrhizin and silybin markedly inhibited $CCl_4-induced$ cytotoxicities. These results demonstrated that the screening method using $CCl_4-induced$ injury in primary cultured rat hepatocytes might be suitable in vitro assay for antihepatotoxic activity.

• Molecules and Cells
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• 제46권6호
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• pp.337-344
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• 2023

N-glycosylation, a common post-translational modification, is widely acknowledged to have a significant effect on protein stability and folding. N-glycosylation is a complex process that occurs in the endoplasmic reticulum (ER) and requires the participation of multiple enzymes. GlcNAc-1-P-transferase (GPT) is essential for initiating N-glycosylation in the ER. Tunicamycin is a natural product that inhibits N-glycosylation and produces ER stress, and thus it is utilized in research. The molecular mechanism by which GPT triggers N-glycosylation is discussed in this review based on the GPT structure. Based on the structure of the GPT-tunicamycin complex, we also discuss how tunicamycin reduces GPT activity, which prevents N-glycosylation. This review will be highly useful for understanding the role of GPT in the N-glycosylation of proteins, as well as presents a potential for considering tunicamycin as an antibiotic treatment.

• 대한한의학회지
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• 제17권2호
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• pp.214-226
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• 1996

In an attempt to evaluate the effect of high and low concentration of Injinsaryungsan and high and low concentration of Sosihotang on cholestatic liver injery induced by $ANIT({\alpha}-naphthylisothiocyanate)$, biochemical changes in serum transaminase(GOT, GPT), alkaline phosphate, lactate dehydrogenase, total cholesterol, triglyceride, total-bilirubine were studied and the following results were obtained. 1. High concentration of Injinsaryungsan(2.2g／Kg) inhibited significantly the activity increases of GOT, GPT, ALP, LDH, TC, TG, T-Bilirubine induced by $ANIT({\alpha}-naphthylisothiocyanate)$. 2. Low concentration of Injinsaryungsan(1.1g／Kg) inhibited the activity increases of ALP, LDH, TC, TG with statistical significance, while inhibited the activity increase of GOT ,but with no statistical significance. 3. High concentration of Sosihotang(2.4g／Kg) inhibited the activity increases of LDH, TG, TC with statistical significance while inhibited the activity increases of GOT, GPT, ALP, T-bilirubine with no significance. 4. Low concentration of Sosihotang(1.2g／Kg) inhibited the activity increase of TG, while inhibited the activity increase of ALP, TC with no statistical sig-nificance, but didn't inhibite the activity increases of GOT, GPT, LDH, T-Bil. These results suggest that Injinsaryungsan has more significant effect on the liver injury induced by $ANIT({\alpha}-naphthylisothiocyanate)$ compared with Sosihotang and so can be applicable clinically to virus hepatitis and cholestatic liver injury. Further study will be required to evaluate the effect of Sosibotang on cholangitis and cholecystitis.

• 대한수의학회지
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• 제15권1호
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• pp.23-25
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• 1975

Eight cattle and ten sheep were administrated various degree of PMA-B and EPN, and S-GOT, S-GPT and serum cholinesterase activity were tested. Serum cholinesterase activity showed no typical tendency, however, S-GOT/S-GPT ratios were decreased by the administration of PMA-B. It was suggested some degree of liver damage by the administration of the chemicals was recognized.

• Toxicological Research
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• 제7권2호
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• pp.165-171
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• 1991

Effects of forced physical exercise on the CCl4 hepatotoxicity were examined in adult female rats. Rats were treated with CCl4 (2 mmol/kg, ip) and introduced into a cylindrical cage rotating at 9 rpm for 20 min/hr for 6 hr. Eighteen hr following the termination of exercise serum sorbitol dehydrogenase (SDH), glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT) activities and glucose-6-phosphatase activity were determined as parameters for hepatotoxicity. Physical exercise inhibited the $CCI_{4-}$induced increases in SDH, GOT, GPT activity, and glucose-6-phosphatase activity were determined as parameters for hepatotoxicity.

• 대한방사선치료학회지
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• 제5권1호
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• pp.120-125
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• 1992

홍삼 추출물의 방사선(5Gy)에 조사된 생쥐의 효소활성에 미치는 영향을 관찰하기 위하여 ICR계 생쥐를 대상으로 생리적 식염수구를 대구조로 하여, 홍삼 엑기스를 살균수 $10m{\ell}$에 50mg에 녹여, 매일 각각 $0.1m{\ell}$씩 10일간 복강내에 홍삼추출물 투여구와 방사선만을 조사한구 및 홍삼추출물 투여 후 방사선 조사구등 4그로 나누어 1일, 4일, 9일, 17일 및 30일 등 5회에 걸쳐 생체의 조직 중 간, 신장에서의 GOT, GPT의 활성을 측정하여 결과를 얻었다. GOT의 활성도 변화는 간, 신장에서 초기에 현저히 증가했으며 이후 회복하는 경향이었다. 방사선 조사구들에서는 홍삼추출물 투여구가 방사선만 조사한구보다 빠르게 회복하는 경향이었다. GPT 활성도 변화는 간, 신장에서 모두 초기에 증가하다가 점차 회복하는 경향이었으며 홍삼추출물 투여 후 방사선 조사구가 방사선만 조사한구보다 빠르게 회복하는 경향이었다. 이러한 결과로 볼 때 홍삼 추출물은 방사선 조사에 의하여 기인된 간 및 신장의 GOT와 GPT 활성도 회복에 효과가 있다고 생각된다.

• 대한수의학회지
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• 제28권1호
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• pp.199-202
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• 1988

The present study was carried out to investigate the serum enzyme activities of racehorses. The enzymes investigated were aspartate and alanine aminotransferase (GOT, GPT), ${\gamma}$-glutamyl transpeptidase(${\gamma}$-GTP), Lactic dehydrogenase(LDH), creatine phosphokinase(CPK), and alkaline phosphatase(ALP). Animals used were 30 healthy racehorses(♀17, ♂13) average weighing 435kg and were from 2 to 8 years of age. LDH activity was progressively decreased with age and next in the order of GOT and CPK activities. GOT and ${\gamma}$-GPT activities were not changed with age but ALP activity tended to be decreased wish age. Activities of GOT, GPT, ${\gamma}$-GTP and ALP were higher in female than in male. There was no difference in CPK activity by sex, and no difference was found out among breeds.