• 대한수의학회지
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• 제31권3호
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• pp.311-318
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• 1991

The DNA fragment representing for Pseudorabies gp50 and gp60(Shope) was cloned by recombinant techniques. The viral DNA was extracted from the infected cells and digested with Bam HI. The 6.8 Kb of Bam HI fragment was isolated from agarose gel and further digested with Nde I followed by Klenow treatment. The blunt ended 4.9Kb fragment was cloned into pTZ18R plasmid vector. The upstream region of gp50 was further manipulated to remove its 5' promoter region and create EcoRl site for possible eukaryotic expression system. The result of partial sequencing of cloned DNA indicated that Shope strain showed 95% homology with gp50 of Rice strain.

• Journal of Pharmaceutical Investigation
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• 제40권6호
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• pp.363-366
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• 2010

Identification of compounds that function as P-glycoprotein (P-gp) substrates or inhibitors can facilitate the selection and optimization of new drug candidates. The purpose of this study is to optimize the experimental conditions for in vitro P-gp assay using LLC-GA5 cells, which is a well-known transformant cell line derived by transfecting LLC-PK1 with human MDR1. The amount of rhodamine123 transported by the LLC-GA5 and LLC-PK1 cells was evaluated under the following experimental conditions: 3 different types of transport media, colchicine pretreatment or nontreatment of the cells in the culture media, and with and without poly-L-lysine coating of the culture plates. The assay sensitivity was found to considerably differ depending on the diluents used in the transport media. P-gp-mediated transport in LLC-GA5 cells was most clearly characterized in the Hanks' balanced salt solution based transport media. The sensitivity of P-gp-mediated transport was not changed by colchicine pretreatment or poly-L-lysine coating of the culture plates.

• Plant Resources
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• 제2권2호
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• pp.88-95
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• 1999

These experiments were conducted to evaluate the variability of seed germination and seedling growth with different levels of polyethylene glycol(PEG 6,000) solution in onion seed. Average germination percentage of seed primed in PEG solution with 1.00 and 0.75 MPa was higher than control, and that of seed primed in 1.50MPa was lower than unprimed control. Germination percentage(GP) of seed primed for 5 days was highest, and as the primed days become long, the GP was decreased. The GP of airation seed during the primed was higher than that of unairation seed, about 5% , respectively. The GP of washed seed after primed was higher than that of unwashed seed, but that of redried seed after primed was lower than that of the others. The highest GP cultivar was Chunjoogoohyung and the lowest GP cultivar was Seouldego in unredried seed after primed, but Chunjoojoonggo was highest and Jungpoonwhang was the lowest cultivar in redried seed after primed. As the PEG concentration increased, the seedling length(SL) was shortened, and seed primed for 15 days was longer than other treatments. The SL of primed seed was similar to GP. The SL of washed seed after primed was longer than that of others, but that of redried seed after primed was shortest among the others. The SL of Chunjoojoonggo and Nongwoodego was longest and Seouldego was shortest among the cultivars in unredried seed after primed, but that of Chunjoogoohyung and Chunjoojoonggo was longest and Seouldego was the shortest cultivars in redried seed after primed.

• 동아시아식생활학회지
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• 제12권2호
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• pp.135-140
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• 2002

1. 관능검사 결과 색은 녹차가루 1.5% 첨가한 청포묵과 무첨가 청포묵이 각각 6.00과 5.82로 높은 선호도를 나타내었고 이들 시료간의 유의적인 차이는 없었다. 향미와 전반적인 기호도는 녹차가루를 1.0%, 1.5%, 0.5%, 무첨가 청포묵 순으로 높게 나타났으나 시료간의 유의적인 차이는 없었다. 맛은 녹차가루를 0.5%와 1.0%첨가한 청포묵이 가장 높은 선호도를 나타내었으며 무첨가 청포묵, 2.0%, 2.0%, 2.5%, 3.0% 순으로 낮은 선호도를 나타내었다. 2. 녹차가루 첨가 청포묵의 색도는 녹차가루를 첨가할수록 L값(명도)은 낮아지는 경향이었고, a (적색도)값은 점점 감소하여 적색은 감소하고 녹색이 증가된 것으로 나타났으며 b(황색도)값은 서서히 증가하여 황색이 증가된 것으로 나타났다. 3. 경도는 녹차가루 2.0%를 첨가한 청포묵이 4617로서 가장 높은 값을 나타내었고 녹차가루를 3.0% 첨가하여 만든 청포묵이 2220으로 가장 낮은 값을 나타내었다. 응집성은 녹차기루 2.5% 첨가한 청포묵이 가장 높은 값을 나타내었으며 1.0% 첨가한 청포묵이 가장 낮은 값을 나타내었다. 점성은 녹차가루 2.0% 첨가한 청포묵이 83.24로 가장 높은 값을 나타내었고 1.5%, 무첨가, 1.0% 청포묵 순으로 낮아졌다. 부서짐성은 2.5% 첨가한 청포묵이 95.52로 가장 높은 값을 나타내었고 0.5% 청포묵이 42.44로 가장 낮은 값을 나타내었다.

• 대한수의학회지
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• 제35권2호
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• pp.287-295
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• 1995

Molecular cloning and nucleotide sequencing were undertaken for the RNA genome of gp53 antigenic region in cytopathic Singer strain of bovine viral diarrhea virus. The cloned cDNA was 939 nucleotides in length having a base composition of 31.0% A, 19.6% C, 25.5% G and 24.0% T. The sequence was corresponded to approximately 77.8%(817 bases) of predicted gp53 region and 122 bases after 3'end of gp53 region in the Singer strain when compared with NADL strain of known sequence. A single open reading frame was found in the sequence of 2nd frame and was deduced as encoding 312 amino acids.

• 약학회지
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• 제54권4호
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• pp.258-269
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• 2010

As people are easy to access the National Health Insurance, medical health service has been increased. It contributed to extend human's average life expectancy and to get better health care. But also increased unnecessary health service or inappropriate drug use. Therefore, DUR (Drug Use Review) is needed to induce appropriate drug use. The purpose of this study is to evaluate outpatient prescriptions by General Practitioner (GP) and Specialized Practitioner, especially indication for ENT referral including common cold which is the frequent indications that have patient see doctor. This study was reviewed retrospectively prescriptions for ENT referral collected at the A pharmacy for ENT Clinic in Cheong-Ju, B pharmacy for GP Clinic in BoEun from Feb 2nd, 2009 to Feb 28th, 2009. Each pharmacy located closed to the each enrolled clinic. The numbers of collected prescriptions were each A pharmacy (n=2501), B pharmacy (n=1343). This study was classified Drug Related Problems (DRPs) those prescriptions had as total 6 groups according to following 6 categories; 1) Unnecessary Drug, 2) Wrong Drug, 3) Low Dose, 4) Overdose, 5) Wrong Instruction, 6) Wrong Combination. In results, Specialized Practitioner's prescriptions had more DRPs than General Practitioner's prescriptions (ENT 155.34% vs GP 130.01%). In detail, Specialized Practitioner's prescriptions had more DRPs in Low Dose (ENT 16.95% vs GP 4.77%), Overdose (ENT 6.72% vs G.P 5.51%), Wrong Instruction (ENT 7.91% vs GP 5.81%), Wrong Combination (ENT 29.31% vs GP 25.09%). These DRPs would be caused from lack of consideration for dosage and drug interaction. General Practitioner's prescriptions had more DRPs in Unnecessary Drug (ENT 70.37% vs GP 78.85%), Wrong drug (ENT 4.12% vs GP 9.98%). These DRPs would be associated with drug selection. This study was assumed that Specialized Practitioner is better prescriber than General Practitioner because Specialized Practitioner complete additional intern and residency training. But, Specialized Practitioner is not always better prescriber than General Practitioner. Furthermore, prescriptions of both Specialized Practitioner and General Practitioner had many problems. In conclusion, It could be cut down the excessive medical expense and expected more efficient medical care by reducing DRPs, thus contributing to the improvement of national health. In order to pharmacist must have good professional ability of pharmacotherapy to help the physician for the drug selection.

• 정보학연구
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• 제6권3호
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• pp.65-73
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• 2003

본 논문에서는 IBM의 고성능 임베디드(embedded) 프로세서인 405GP와 Linux 2.4.21를 이용하여 5.2GHz 대역에서 최대 54Mbps의 대역폭을 제공할 수 있는 IEEE 802.11a 기반의 AP (Access point) 개발에 관하여 연구하였다. AP의 하드웨어는 PowerPC기반의 IBM 405GP 프로세서를 기반으로 설계하였고 AP 소프트웨어는 405GP을 위한 PowerPC 부트로더, open source인 최근 Linux 커널 2.4.21을 사용하여 구현하였다. 또한 시스템 패키징은 최신 Linux 커널이 지원하는 MTD 기술과 JFFS2 플래쉬 파일시스템을 이용하여 최적으로 구현하였다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.87-87
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• 2018

This study was conducted to investigate the effects of osmotic priming, liquid smoke (LS), sonication and modified drum priming treatments to improve the germination speed and uniformity of pepper seeds for high quality export seeds. Seeds were treated in 0.5, 1.0, 5.0, 10% LS solution only or with 100mM $K_2SO_4$ solution for 6days at $15^{\circ}C$. Sonication treatment was performed for 5, 10, and 20 minutes at an intensity of 5.2, 10.4, 15.7, 21.0, 26.1kHz in water at $15^{\circ}C$. After sonication treatment, seeds were primed with water or 100mM $K_2SO_4$ for 4days. 40, 50 and 60% seed moisture content (SMC) of hydrated seeds were incubated for 84, 96 and 108h in a container with a relative humidity of 99% at 26rpm for a modified drum priming treatments. 0.5% LS treatment with water or 100mM $K_2SO_4$ priming showed significant effects with 71% GP (9.0days MGT) and 66% GP (7.4days MGT), respective, while untreated seeds resulted in only 61% GP and 10days MGT. The healthy seed % was increased by 24% by $K_2SO_4$ priming with LS treatment compared to untreated seeds. However, sonication treatment showed less germination or no difference compared to untreated seed, regardless of intensity and treatment time. The modified drum priming treatment (108h incubation after 50% SMC hydration) significantly improved the GP (on the $4^{th}$days after sowing), germination rate (GR), and MGT to 65%, $28%{\cdot}day^{-1}$, and 3.6days, whereas untreated seed resulted only 7% GP, $17%{\cdot}day^{-1}$ GR and 5.9days MGT. Thus, modified drum priming treatment effectively improved seed germination and is considered as an industrially promising treatment methods considering the shortening of the treatment period and environment-friendly aspects.

• 한국재료학회:학술대회논문집
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• 한국재료학회 2009년도 추계학술발표대회
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• pp.31.2-31.2
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• 2009

본 연구에서는 Graphene oxide를 $800^{\circ}C$, 질소분위기 하에서 환원시켜 얻은 $GP_{TR}$과 Hydrazine을 이용한 화학적 처리로 얻은 $GP_{CR}$의 분자구조를 제안하였다. 이때 grapheneoxide는 modified Hummers' method를이용하여 제조하였다. $GP_{TR}$과 $GP_{CR}$은 모두 표면에 몇몇의 oxide group이 존재하는데$GP_{TR}$은 six layer로 $C_{100}O_{3{\pm}1}$ 의 조성을 갖고 $GP_{CR}$은 three layer로 and $C_{100}O_{6.5{\pm}2}$의 조성을 갖는다. 그리고 면간간격은 $GP_{TR}$은 $3.432\;{\AA}$, $GP_{CR}$은 $3.760\;{\AA}$으로 전형적인 방법인 top downprocess로 얻은 graphene 보다 다소 크다는 것을 알 수 있다.

• 미생물학회지
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• 제55권2호
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• pp.103-111
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• 2019

DNA 메틸화는 유전체의 무결성의 유지 및 유전자 발현 조절과 같은 박테리아의 다양한 과정에 관여한다. Alphaproteobacteria 종에서 보존된 DNA 메틸 전이 효소인 CcrM은 S-아데노실 메티오닌을 공동 기질로 사용하여 $N^6$-아데닌 또는 $N^4$-시토신의 메틸 전이 효소 활성을 갖는다. Celeribacter marinus IMCC 12053는 해양 환경에서 분리된 알파프로테오박테리아로서 GpC 시토신의 외향고리 아민의 메틸기를 대체하여 $N^4$-메틸 시토신을 생산한다. 단일 분자 실시간 서열 분석법(SMRT)을 사용하여, C. marinus IMCC12053의 메틸화 패턴을 Gibbs Motif Sampler 프로그램을 사용하여 확인하였다. 5'-GANTC-3'의 $N^6$-메틸 아데노신과 5'-GpC-3'의 $N^4$-메틸 시토신을 확인하였다. 발현된 DNA 메틸전이 효소는 계통 발생 분석법을 사용하여 선택하여 pQE30 벡터에 클로닝 후 $dam^-/dcm^-$ 대장균을 사용하여 클로닝된 DNA 메틸라아제의 메틸화 활성을 확인하였다. 메틸화 효소를 코딩하는 게놈 DNA 및 플라스미드를 추출하고 메틸화에 민감한 제한 효소로 절단하여 메틸화 활성을 확인하였다. 염색체와 메틸라아제를 코드하는 플라스미드를 메틸화시켰을 때에 제한 효소 사이트가 보호되는 것으로 관찰되었다. 본 연구에서는 분자 생물학 및 후성유전학을 위한 새로운 유형의 GpC 메틸화 효소의 잠재적 활용을 위한 외향고리 DNA 메틸라제의 특성을 확인하였다.