• IMMUNE NETWORK
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• v.22 no.6
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• pp.51.1-51.20
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• 2022

Trypanosoma cruzi, the etiological agent of Chagas disease, is an intracellular protozoan parasite, which is now present in most industrialized countries. About 40% of T. cruzi infected individuals will develop severe, incurable cardiovascular, gastrointestinal, or neurological disorders. The molecular mechanisms by which T. cruzi induces cardiopathogenesis remain to be determined. Previous studies showed that increased IL-6 expression in T. cruzi patients was associated with disease severity. IL-6 signaling was suggested to induce pro-inflammatory and pro-fibrotic responses, however, the role of this pathway during early infection remains to be elucidated. We reported that T. cruzi can dysregulate the expression of host PIWI-interacting RNAs (piRNAs) during early infection. Here, we aim to evaluate the dysregulation of IL-6 signaling and the piRNAs computationally predicted to target IL-6 molecules during early T. cruzi infection of primary human cardiac fibroblasts (PHCF). Using in silico analysis, we predict that piR_004506, piR_001356, and piR_017716 target IL6 and SOCS3 genes, respectively. We validated the piRNAs and target gene expression in T. cruzi challenged PHCF. Secreted IL-6, soluble gp-130, and sIL-6R in condition media were measured using a cytokine array and western blot analysis was used to measure pathway activation. We created a network of piRNAs, target genes, and genes within one degree of biological interaction. Our analysis revealed an inverse relationship between piRNA expression and the target transcripts during early infection, denoting the IL-6 pathway targeting piRNAs can be developed as potential therapeutics to mitigate T. cruzi cardiomyopathies.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.29 no.3
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• pp.237-247
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• 2011

As of 2011, 72 Permanent GPS Stations are installed to control DGPS reference points by the National Geographic Information Institute in South Korea. As the center of the Earth's mass continues to move, the coordinates of the permanent GPS stations become inconsistent over time. Thus, a reference frame using a set of coordinates and their velocities of a global network of stations at a specific period has been used to solve the inconsistency. However, the relative movement of the permanent GPS stations can lower the accuracy of GPS relative positioning. In this research, we first analyzed the data collected daily during the past 30 months at the 40 permanent GPS stations within South Korea and the 5 IGS permanent GPS stations around the Korean Peninsula using a global network adjustment. We then calculated the absolute and relative amount of movement of the GPS permanent stations. We also identified the optimum renewal period of the permanent GPS stations considering the accuracy of relative GPS surveying. Finally, we developed a Korean a Korean crustal movement model that can be used to improvement of accuracy.

• Journal of Korean Society for Geospatial Information Science
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• v.19 no.4
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• pp.119-126
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• 2011

During precise survey on the top of High rise buildings and civil structures, optical surveying equipments like a Total Station are not recommended to use because of some reasons that uneasier alignment with reflectors located at the top of building, increasing error depends on increasement of observation distance and unavailable dynamic positioning etc. Recently various GPS positioning methods have been applied to this job however almost of them are post-processing method which is required much longer time during for whole process includes stake-out, cross checking, fixing positions and final inspections. Therefore, in this study, we applied with RTK surveying system which allows stake-out and inspection in realtime to avoid delaying of construction schedule and also applied with Quasi Static RTK measurement and network adjustment to get a high accuracy within a few millimeters in structure positioning to achieve a successful management for process and quality control of the project. As a result, very high accurate surveying for structures within approx. 2mm in realtime has been achieved when surveyor conduct a network adjustment using least square method for 4 base lines created by Quasi Static RTK data and we expect this method will be applied to construction survey for high rise buildings and civil structures in the future.