• Title/Summary/Keyword: GOx

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Development of miniaturized glucose sensor based on glucose oxidase immobilized on polypyrrole-ferricyanide films on platinum electrodes (소형화된 glucose 센서 제작 및 전기 화학적 특성 분석)

  • Yun, Dong-Hwa;Yang, Jung-Hoon;Jin, Joon-Hyung;Min, Nam-Ki;Hong, Suk-In
    • Proceedings of the KIEE Conference
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    • 2002.11a
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    • pp.248-250
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    • 2002
  • 본 논문은 당뇨병의 지표물질인 glucose의 농도를 극미량의 시료를 사용하여 정량 할 수 있는 방법을 개발하기 위하여 효소 고정화 전극을 제작하였다. 전극은 실리콘 웨이퍼상에 마이크로 크기의 전극을 반도체 공정을 이용하여 제작하였고, 전기 화학적 방법으로 마이크로 전극에 전도성 고분자 Polypyrrole(PPy) 및 glucose oxidase(GOx)를 고정화한 고감도의 전기화학 전극을 개발하였다. 도전성 고분자의 전기 화학적 중합은 순환 전압 전류법으로 하였으며, 용액의 액성에 따른 효소의 표면 전하를 이용하여, 도전성 고분자를 코팅한 전극에 일정한 전압을 인가하고 GOx를 도우핑 하였다. 제작된 전극은 시간대 전류법으로 glucose의 농도에 따른 감도 측정결과 마이크로 리터의 시료에 $5{\mu}A$/decade를 얻었다. 전극의 표면분석은 Scanning electron microscopy(SEM), Energy dispersive X-ray spectroscopy(EDX)를 이용하였다.

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The Interaction of Vortex Shedding Behavior in Hybrid Rocket Combustion (와류간섭에 의한 하이브리드로켓 연소 특성)

  • Park, Kyung-Soo;Lee, Chang-Jin;Shin, Kyung-Hoon
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 2012.05a
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    • pp.244-248
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    • 2012
  • A series of hybrid rocket combustion experiments were carried out with PMMA/GOx changing diameter and length of the disk installed at pre-chamber. The disk can generate vortex shedding flow and change flow conditions prior to entering the fuel grain which could also alter the combustion characteristics and pressure oscillations. The interaction of vortex shedding in the pre-chamber and small-scale vortices adjacent to burning surfaces by using combustion test.

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Quantitative analysis of the errors associated with orbit uncertainty for FORMOSAT-3

  • Wu Bor-Han;Fu Ching-Lung;Liou Yuei-An;Chen Way-Jin;Pan Hsu-Pin
    • Proceedings of the KSRS Conference
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    • 2005.10a
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    • pp.87-90
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    • 2005
  • The FORMOSAT-3/COSMIC mission is a micro satellite mission to deploy a constellation of six micro satellites at low Earth orbits. The final mission orbit is of an altitude of 750-800 lan. It is a collaborative Taiwan-USA science experiment. Each satellite consists of three science payloads in which the GPS occultation experiment (GOX) payload will collect the GPS signals for the studies of meteorology, climate, space weather, and geodesy. The GOX onboard FORMOSAT -3 is designed as a GPS receiver with 4 antennas. The fore and aft limb antennas are installed on the front and back sides, respectively, and as well as the two precise orbit determination (POD) antennas. The precise orbit information is needed for both the occultation inversion and geodetic research. However, the instrument associated errors, such as the antenna phase center offset and even the different cable delay due to the geometric configuration of fore- and aft-positions of the POD antennas produce error on the orbit. Thus, the focus of this study is to investigate the impact of POD antenna parameter on the determination of precise satellite orbit. Furthermore, the effect of the accuracy of the determined satellite orbit on the retrieved atmospheric and ionospheric parameters is also examined. The CHAMP data, the FORMOSAT-3 satellite and orbit parameters, the Bernese 5.0 software, and the occultation data processing system are used in this work. The results show that 8 cm error on the POD antenna phase center can result in ~8 cm bias on the determined orbit and subsequently cause 0.2 K deviation on the retrieved atmospheric temperature at altitudes above 10 lan.

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A glucose biosensor based on deposition of glucose oxidase onto Au nanoparticles poly(maleic anhydride)-grafted multiwalled carbon nanotube electrode (금 나노입자/폴리(maleic anhydride) 그래프트 탄소나노튜브에 글루코스 옥시다아제 담지를 기반으로 한 글루코스 바이오센서)

  • Piao, Ming-Hua;Son, Pyeong-Soo;Chang, Choo-Hwan;Choi, Seong-Ho
    • Analytical Science and Technology
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    • v.23 no.2
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    • pp.165-171
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    • 2010
  • Glucose oxidase ($GOD_{ox}$) immobilized biosensor was fabricated by two methods. In one of the methods, gold nanoparticles (Au-NPs) prepared by ${\gamma}$-irradiation were loaded into the poly(maleic anhydride)-grafted multi-walled carbon nanotube, PMAn-g-MWCNT electrode via physical entrapment. In the other method, the Au-NPs were prepared by electrochemical reduction of Au ions on the surface of PMAn-g-MWCNT electrode and then GODox was immobilized into the Au-NPs. The $GOD_{ox}$ immobilized biosensors were tested for electrocatalytic activities to sense glucose. The sensing range of the biosensor based on the Au-NPs physically modified PMAn-g-MWCNT electrode was from $30\;{\mu}M$ to $100\;{\mu}M$ for the glucose concentration, and the detection limit was $15\;{\mu}M$. Interferences of ascorbic acid and uric acid were below 7.6%. The physically Au deposited PMAn-g-MWCNT paste electrodes appear to be good sensor in detecting glucose.

Enzyme Sensors Modified with Avidin/Biotin Systembased Protein Multilayers

  • Anzai, Jun-Ichi;Du, Xiao-Yan;Hoshi, Tomonori;Suzuki, Yasuhiro;Takeshita, Hiroki;Osa, Tetsuo
    • Analytical Science and Technology
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    • v.8 no.4
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    • pp.591-596
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    • 1995
  • Enzyme multilayers composed of avidin and biotin-labeled enzymes were prepared on the surface of electrode, through a strong affinity between avidin and biotin (binding constant: ca $10^{15} M^{-1}$). The enzyme multilayers were useful for the improvement of the performance characteristies of enzyme sensors. The output current of the enzyme sensors depended linearly on the number of enzyme layers deposited. Thus, lactate oxidase (LOx) and alcohol oxidase (AlOx) were deposited after being modified with biotin for constructing enzyme sensors sensitive to L-lactate and ethanol respectively. It was also possible to deposit two different kinds of enzymes successively in a single multilayer. The glucose oxidase (GOx) and ascorbate oxidase (AsOx) were built into a multilayer structure on a Platinum electrode. The GOx, AsOx multilayer-modified electrode was useful for the elimination of ascorbic acid interference of the glucose sensor.

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Visualization device of solid fuel combustion in hybrid rocket (하이브리드 로켓에서의 고체 연료 연소 가시화 장치)

  • Moon, Keun-Hwan;Cho, Jung-Tae;Kim, Soo-Jong;Lee, Jung-Pyo;Kim, Hak-Chul;Oh, Ji-Sung;Moon, Hee-Jang;Sung, Hong-Gye;Kim, Jin-Kon
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 2010.05a
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    • pp.206-209
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    • 2010
  • The visualization device for hybrid rocket is fabricated to investigate the combustion phenomena. Visualization device were composed with ignition system, oxidizer supply system, control system and data acquisition system, combustion visualization system. GOX as oxidizer and HDPE, Paraffin-LDPE Blending, Paraffin sd were used. As results, combustion phenomena and fuel droplet entrainment were observed.

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The Patterns of Streamwise Vortex on the Fuel Surface in Hybrid Rocket Combustion (하이브리드 로켓 모터 연소 중 발생하는 streamwise 와류 특성)

  • Shin, Kyung-Hoon;Park, Kyung-Su;Mon, Khin Oo;Lee, Chang-Jin
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 2011.11a
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    • pp.649-652
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    • 2011
  • A series of hybrid rocket combustion experiments were carried out with PMMA/GOx changing diameter and length of the disk installed at pre-chamber. The disk can generate vortex shedding flow and change flow conditions prior to entering the fuel grain which could also alter the combustion characteristics and pressure oscillations. Isolated dimple-like surface roughness patterns distributed all over the fuel surface, which can be thought of as a realization of the inherent flow instability. It is very likely that the formation of cell structures is originated from the modification of boundary layer characteristics of an entering oxidizer flow caused by a blowing effect mainly taking place near the wall. This coincided with our LES results. It would be a meaningful basis to understand combustion instability of hybrid rocket motor.

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Preparation of Enzyme Electrodes for Biofuel Cells Based on the Immobilization of Glucose Oxidase in Polyion Complex (폴리이온복합체를 이용하여 글루코스 산화효소를 고정화한 바이오전지용 효소전극 제조)

  • Nguyen, Linh Thi My;Li, Nan;Yoon, Hyon Hee
    • Applied Chemistry for Engineering
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    • v.24 no.1
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    • pp.99-103
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    • 2013
  • An emzymatic bioanode for a glucose/oxygen biofuel cell was prepared by the sequential coating of carbon nanotube (CNT), charge transfer complex (CTC) based on tetracyanoquinodimethane (TCNQ) and tetrathiafulvalene (TTF), glucose oxidase (GOx), and polyion complex (mixture of poly-L-lysine hydrobromide and poly (sodium 4-styrenesulfonate)) on a glassy carbon electrode. A biocathode was also prepared by the sequential coating of CNT, bilirubin oxidase (BOD), 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), and polyion complex. The effect of CNT and CTC on the electrochemical performance was investigated. The biofuel cell exhibited a promising performance with maximum power densities of 3.6, 10.1, and $46.5{\mu}W/cm^2$ at 5, 20, and 200 mM of glucose concentration, respectively. The result indicates that the biofuel cell architecture prepared in this study can be used in the development of biofuel cells and biosensors.

A Study on Combustion Characteristics of Paraffin Wax Fuel for Content of Micron-sized Aluminum Particles (마이크로 알루미늄 입자 함유량에 따른 파라핀 연료의 연소 특성 연구)

  • Park, Younghoon;Ryu, Sunghoon;Han, Seongjoo;Moon, Heejang;Kim, Jinkon;Kim, Junhyung;Ko, Seungwon
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 2017.05a
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    • pp.489-494
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    • 2017
  • This paper describes the combustion characteristics of aluminized paraffin fuel on the contents of micron-sized aluminum particles with nominal diameters of $8{\mu}m$. Aluminized paraffin fuels with mixture ratio of aluminum 0 wt%, 5 wt% and 10 wt% as fuel and GOx(Gaseous Oxygen) as oxidizer were used to perform the experiments. The experimental investigations were performed on the regression rate, the chamber pressure and the combustion efficiency. Increasing a content of micron-sized aluminum particles, the results of regression rate, chamber pressure and combustion efficiency show minor increase compared to those without particles.

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