• Title/Summary/Keyword: GOT

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Studies on the amino acid metabolism of young rice root (Part 4) -Properties of glutamic oxalacetic transaminase isozymes of rice root- (수도근(水稻根)의 Amino 산(酸) 대사(代謝)에 관(關)한 연구(硏究) -제(第) 4 보(報) 수도근(水稻根)의 Glutamic Oxalacetic Transaminase Isozyme의 성질(性質))

  • Kim, Kwang Sik;Kim, Young Ung
    • Korean Journal of Soil Science and Fertilizer
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    • v.7 no.4
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    • pp.209-213
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    • 1974
  • The isozymes of glutamic oxalacetic transaminase from young rice root were isolated by DEAE-cellulose column chromatography, and investigated some of their enzymic properties. Obtained results were summarized as follows: 1. 93 percent of GOT activity in rice root existed in supernatant fraction, and their specific activity exibited the same pattern. 2. The rice root contained two types of GOT isozyme; cationic and anionic GOT. It seemed that both of them were distributed in supernatant fraction. 3. GOT isozymes of rice root on the pH dependance showed their maximum activity at 7.4. 4. On the stability of GOT isozymes for temperature, anionic GOT was more unstable than cationic GOT. 5. Apparent Michaelis constant for L-aspartate of GOT isozymes from rice root showed 12-14mM in the cationic GOT and 16mM in the anionic GOT, respectively.

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Study on the Activity of GOT and GPT in the Hepatotoxic Rat Treated (구기자 투여 간손상 흰쥐에서 GOT 및 GPT의 활성화 연구)

  • 김병원;노광수
    • Biomedical Science Letters
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    • v.6 no.3
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    • pp.187-192
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    • 2000
  • The present study was undertaken in order to investigate betaine production by tissue culture and its medicinal effect in Lycium chinone Mill. In order to ulvestigate the protective effect of L. chinense on the hepatoxicity induced by $CCl_4$, 0.5 g/kg water extract of the compound mixture (leaves, roots and shoots) of L. chinense and its callus were fed to rat (SD line) once a day. As a result, the activity of GOT and GPT in the group fed compound mixture (GOT 760.4 and GPT 540 Karmen unit) and callus (G0T 772.1 and GPT 556.4 Karmen unit) was decreased in the blood serume relative to the controlled rat group (GOT 949 and GPT 640 Karmen unit) and the same result was obtained in the group fed with 0.1 g/kg sylimarin (the activity of GOT and GPT was shown 492.6 and 320.4 Karmen unit respectively. These results strongly indicate that water extracts of the mixture and callus from L. chinense do have the same decreasing effect of GOT and GPT in the hepatotoxic rat induced by $CCl_4$.

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CFI Approach to Defend against GOT Overwrite Attacks (CFI(Control Flow Integrity) 적용을 통한 GOT(Global Offset Table) 변조 공격 방지 방안 연구)

  • Jeong, Seunghoon;Hwang, Jaejoon;Kwon, Hyukjin;Shin, Dongkyoo
    • Journal of Internet Computing and Services
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    • v.21 no.1
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    • pp.179-190
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    • 2020
  • In the Unix-like system environment, the GOT overwrite attack is one of the traditional control flow hijacking techniques for exploiting software privileges. Several techniques have been proposed to defend against the GOT overwrite attack, and among them, the Full Relro(Relocation Read only) technique, which blocks GOT overwrites at runtime by arranging the GOT section as read-only in the program startup, has been known as the most effective defense technique. However, it entails loading delay, which limits its application to a program sensitive to startup performance, and it is not currently applied to the library due to problems including a chain loading delay problem caused by nested library dependency. Also, many compilers, including LLVM, do not apply the Full Relro technique by default, so runtime programs are still vulnerable to GOT attacks. In this paper, we propose a GOT protection scheme using the Control Flow Integrity(CFI) technique, which is currently recognized as the most suitable technique for defense against code reuse attacks. We implemented this scheme based on LLVM and applied it to the binutils-gdb program group to evaluate security, performance and compatibility. The GOT protection scheme with CFI is difficult to bypass, fast, and compatible with existing library programs.

Genetic Analysis of Some Polymorphic Isozymes in Pinus densiflora (I) -Inheritance of Glutamate-Oxalate Transaminase and Leucine Aminopeptidase, and Linkage Relationship among Allozyme Loci- (소나무의 몇가지 다형적(多形的) 동위효소(同位酵素)의 유전분석(遺傳分析)(I) -Glutamate-Oxalate Transaminase 와 Leucine Aminopeptidase의 유전(遺傳)과 동위효소(同位酵素) 유전자좌(遺傳子座) 간(間)의 연관관계(連關關係)-)

  • Kim, Zin Suh;Hong, Yong Pyo
    • Journal of Korean Society of Forest Science
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    • v.58 no.1
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    • pp.1-7
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    • 1982
  • Megagametophyte and embryo tissue of Pinus densiflora were subjected to study the inheritance of glutamate-oxalate transaminase(GOT) and leucine aminopeptidase(LAP), and linkage relationship among isozyme loci coding both enzymes by starch gel zone-electrophoresis. Four zones of activity were observed for GOT. No variation was found in the fastest migrating zone (GOT-A). Electrophoretic phenotypes of the other two zones (GOT-B and GOT-C) showed 1:1 segregation ration, suggesting that each zone is controlled by a single locus. Foru and three alleles were identified at both loci respectively. The isozyme pattern of the fourth zone(GOT-D), migrated cathodally, coincided precisely with that of GOT-C. Whether the two zones are controlled by the same locus or by two tightly linked loci remained unknown. In all three variant GOT zones, heterozygoes embryos produced triple band patterns, indicating that GOT isozyme in Pinus densiflora is a dimer. Two zones of activity stained for LAP were found. The segregation of the two zones (LAP-A and LAP-B) suggested that tow loci control each of both isozymes. Two and three alleles were identified at both loci. GOT-B and LAP-B were found to be tightly linked, showing an average recombination frequency of 12.5 percent. Slight deviation from independent assortment was observed between GOT-B and GOT-C, with recombination frequency of 41 percent.

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A Study on the Understanding of Aromatherapy of University Students (대학생의 아로마테라피에 대한 인식도 분석)

  • Lee, Ju-Yeon
    • The Research Journal of the Costume Culture
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    • v.18 no.1
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    • pp.150-160
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    • 2010
  • This research was intended to furnish basic data which can be helpful to maintain and improve the skin health and to activate aromatherapy by analyzing the knowledge, attitude and behavior on the aromatherapy skin care of college students. As their grade got higher, students of beauty class showed the level of knowledge on aromatherapy higher than those of non-beauty class, and that of girl students was higher than that of boy students and as the living standard of students got higher, and pocket money of a month of them got more, the level of knowledge on aromatherapy of them was high, and as the interest in appearance and satisfaction on appearance of them got higher, the level of knowledge on aromatherapy of them was high. In common characteristics, as the living standard of them got higher, and pocket money of a month of them got more, the level of attitude on aromatherapy of them was high. As the interest in appearance of them got higher, the level of attitude on aromatherapy on the interest in appearance of them was high. In the characteristics related with the skin, as the skin health condition of students got healthier, the level of attitude on aromatherapy of them was high, and it was high in the students who had much agony on the skin color.

Glutamic-oxaloacetic transaminase 1 regulates adipocyte differentiation by altering nicotinamide adenine dinucleotide phosphate content

  • Yang, Yang;Cheng, Zhimin;Zhang, Wanfeng;Hei, Wei;Lu, Chang;Cai, Chunbo;Zhao, Yan;Gao, Pengfei;Guo, Xiaohong;Cao, Guoqing;Li, Bugao
    • Animal Bioscience
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    • v.35 no.2
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    • pp.155-165
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    • 2022
  • Objective: This study was performed to examine whether the porcine glutamic-oxaloacetic transaminase 1 (GOT1) gene has important functions in regulating adipocyte differentiation. Methods: Porcine GOT1 knockout and overexpression vectors were constructed and transfected into the mouse adipogenic 3T3-L1 cells. Lipid droplets levels were measured after 8 days of differentiation. The mechanisms through which GOT1 participated in lipid deposition were examined by measuring the expression of malate dehydrogenase 1 (MDH1) and malic enzyme (ME1) and the cellular nicotinamide adenine dinucleotide phosphate (NADPH) content. Results: GOT1 knockout significantly decreased lipid deposition in the 3T3-L1 cells (p<0.01), whereas GOT1 overexpression significantly increased lipid accumulation (p<0.01). At the same time, GOT1 knockout significantly decreased the NADPH content and the expression of MDH1 and ME1 in the 3T3-L1 cells. Overexpression of GOT1 significantly increased the NADPH content and the expression of MDH1 and ME1, suggesting that GOT1 regulated adipocyte differentiation by altering the NADPH content. Conclusion: The results preliminarily revealed the effector mechanisms of GOT1 in regulating adipose differentiation. Thus, a theoretical basis is provided for improving the quality of pork and studies on diseases associated with lipid metabolism.

Behavior Problem According to Children's Temperament and Self-Regulation (유아의 기질과 자기조절능력에 따른 문제행동)

  • Jung, Hye-Jin;Lee, Wan-Jeong
    • Journal of Families and Better Life
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    • v.27 no.3
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    • pp.159-170
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    • 2009
  • The purpose of this study was (1) to investigate the difference of temperament, self-regulation, and problem behavior according to children's gender and age, (2) to investigate the relationship among three variables by use of canonical correlation analysis. The subjects were 210 children ages from 3 to 5 in five day-care center in Incheon city. The SPSS WIN 12.0 Program was used to analyze the collected data. The statistical methods were Frequency, Percentage, Cronbach's ${\alpha}$, Two-way ANOVA, Correlation analysis, and Canonical Correlation analysis. The major results of this research are summarized as follows: (1) Children's temperament were significantly different by their gender and age. The boys got higher score than girls in adaptability, activity, and total temperament. Girls got higher score than boys in reactivity. The age 3 got higher score than the age 5 in activity and the ages 3, 4 got higher than the age 5 in total temperament. (2) Children's self-regulation were significantly different by their age. The children who were older got higher scores in self-regulation ability. (3) Children's problems behavior were significantly different by their gender and age. The boys got higher score than girls in anxiety behavior, aggressive behavior, hyperactive/distractive behavior, and total problem behavior. And the ages 3, 4 got higher score than the age 5 in anxiety behavior and hyperactive/distractive behavior. The age 4 got higher score the ages 3, 5 in aggressive behavior and the age 3 got higher score the ages 4, 5 in total problem behavior. (4) Canonical correlation results showed that stable temperament and self-regulation were positively correlated and self-regulation and problem behavior were negatively correlated.

Serum Enzyme (GOT, LDH, ALP) Activities in Relation to Stage of Gestation in Korean Native Cattle (한우(韓牛)의 임신기간(姙娠期間)에 따른 혈청효소(血淸酵素) 활성도(活性度) (GOT, LDH 및 ALP) 의 변화(變化))

  • Jo, Chung-Ho
    • Korean Journal of Veterinary Research
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    • v.22 no.1
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    • pp.75-79
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    • 1982
  • Serum glutamic oxaloacetic transaminase (GOT), latic dehydrgenase (LDH), and alkaline phosphatase (ALP) activities were determined in 5 Korean native cattle to obtain the base line of blood enzyme activities in relation to the stage of gestation. Serum GOT activities showed an increasing tendency with the course of gestation period, serum LDH levels were within normal range through all the stage of pregnancy, and the slight increase in serum ALP activities was acknowledged at the 2nd and 3rd stage of gestation.

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A study on 119 Rescue and First aids' satisfaction level of Occupation (119구조·구급대원의 직업만족도에 관한 연구 -전국을 대상으로-)

  • Kim, Soon-Sim;Kwon, Hayrran
    • The Korean Journal of Emergency Medical Services
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    • v.6 no.1
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    • pp.169-184
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    • 2002
  • The purpose of this study was to find out the 119 rescue and first aid's satisfaction level for their occupations, understand the influential factors of the satisfaction level and also analyze the level of each working districts. Therefore to provide basic information of policy decisions to increase the satisfaction level. Date were collected from 400 national the 119 rescue and first aids by questionnaire and using the tools, which has developed by Slavitt, from August 2 to September 30, 2002. The data were analyzed by percentage, mean t-test and ANOVA. Divided 39 items into 7 provinces to find out the satisfaction level of each province, follow as the influential factors of the satisfaction level. 1. The most of the 119 rescue and first aids were satisfying about their occupations as they got 3.02 marks out of 5, which is the full mark. 2. As the results after analyzed the relation ship between the satisfaction level and the general characteristics of analyzed the relation ship between the satisfaction level and the general characteristics of the 119 rescue and first aids, there were not statistically significant differences of sex distinction, age, scholarship, not married and has or have married, grade and qualification. There were statistically significant differences follow as working period, applying motive, moving, seeking a job, working place, an adviser, work overtime and compensation follow as work overtime. 3. It appeared that all the 119 rescue and first aids were satisfying enough about duty itself but were dissatisfying about working condition. As the results; job itself got 4.00, reciprocal action got 3.74, business demand got 3.28, autonomy got 3.06, organization demand got 2.96, pay got 2.43 and working condition got 1.72, each out of 5 full marks. As you see the results, most of the 119 rescue and first aids are satisfying about a job itself but they are dissatisfying about their working condition. Therefore we should formulate a plan that can increase the satisfaction level by improving duty system for 24 working hours per week.

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Effect of Nonylphenol on Plasma Glutamate Oxaloacetate Transaminase (GOT) and Glutamate Pyruvate Transaminase (GPT) in the Juvenile Rockfish, Sebastes schlegeli

  • Hwang Un-Gi;Kang Ju-Chan
    • Fisheries and Aquatic Sciences
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    • v.5 no.4
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    • pp.308-310
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    • 2002
  • Effect of 4-nonylphenol (4-NP), endocrine disrupting compounds (EDCs), on glutamate oxaloacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT) were investigated in the plasma of juvenile rockfish, Sebastes schlegeli. Fish were injected with 4­NP (10, 50, 100 and 200 mg/kg body weight) in $70\%$ ethanol twice at 3-day intervals and plasma sampling were extracted at 7 days after the last injection. Controls received solvent only. 4-NP significant increased GOT in a dose-dependent manner. GPT was markedly elevated to $61\%$ (P<0.05) and $82\%$ (P<0.01) than that of the control at the 4-NP doses of 100 and 200mg, respectively. These results suggest that the estrogenic activity of 4-NP increase plasma GOT and GPT by toxic effect on hepatocyte.