• Journal of Plant Biotechnology
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• 제5권2호
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• pp.69-77
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• 2003

The ornamental industry encompasses cut flower, pot plant, turfgrass and nursery stock production and is an important part of the agricultural sector. As internationally traded commodities, cut flowers and plants are an integral part of the economy of a number of developing countries in South America, the Caribbean and Africa. Genetic modification (GM) is a tool with great potential to the ornamental horticulture industry. The rapid progress in our knowledge of plant molecular biology can accelerate the breeding ornamental plants using recombinant DNA technology techniques. Not only is there the possibility of creating new, novel products the driver of the industry but also the potential to develop varieties requiring less chemical and energy inputs. As an important non-food agricultural sector the use of genetically modified (GM) ornamental crops may also be ideal for the intensive farming necessary to generate pharmaceuticals and other useful products in GM plants. To date, there are only a few ornamental GM products in development and only one, a carnation genetically modified for flower colour, in the marketplace. International Flower Developments, a joint venture between Florigene Ltd. in Australia and Suntory Ltd. of Japan, developed the GM carnations. These flowers are currently on sale in USA, Japan and Australia. The research, development and commercialization of these products are summarized. The long term prospects for ornamental GM products, like food crops, will be determined by the regulatory environment, and the acceptance of GM products in the marketplace. These critical factors will be analysed in the context of the current legislative environment, and likely public and industry opinion towards ornamental genetically modified organisms (GMO's).

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2015년도 학술발표회
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• pp.378-378
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• 2015

연안지역에서 발생하는 퇴적 및 침식현상으로 발생하는 피해를 분석하기 위하여, 고립파를 이용한 연구가 계속 진행되어왔다. 고립파는 파고의 크기에 따라 파형이 결정되는 특징을 가지고 있어 연안지역 파랑에 관한 연구에 적합하다. 기존연구는 sluice gate를 순간적으로 개방하는 방식을 통해 갑작스러운 수위의 변화를 유도하여 급변부정류흐름을 발생시키는 연구를 수행하였으나, 고립파 발생 조건에 대한 상세한 분석은 수행하지 못하였다. 본 연구는 기존연구에서 사용한 방식과 동일하게 sluice gate를 개방하여 고립파를 발생시켰으며, 실험조건을 상류 headtank 수위와 하류 수심의 수위차를 이용하는 경우와 sluice gate 개방 속도에 따라 두 가지 경우로 나누어 실험을 수행하였다. 실험에 사용된 직사각형 수리실험수조는 폭 0.8 m, 높이 0.75 m, 수로길이 12 m에서 상 하류의 수위차를 0.05 m 단위로 높이는 방식과 수문개방속도를 0.5 m/s 부터 1.0 m/s까지 범위를 설정하여 두 가지 재현방식에 따라 실험을 진행하였다. 또한 Flow-3D GMO(General Moving Objects)기능을 활용한 수치모의를 수행하였다. 실험조건에 따른 수리실험 결과를 파랑의 특성인 고립파 파고와 파속을 수치모의결과와 비교하여 분석하였다. 수리실험결과와 수치해석결과 유사한 경향을 나타내었으며, 본 연구의 결과는 Sluice gate를 이용한 고립파 수리실험으로 경사면에서 발생하는 침식과 퇴적현상에 관한 기초적인 연구가 될 것이다.

• 농업과학연구
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• 제49권4호
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• pp.795-807
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• 2022

The development and commercialization of industrial genetically modified (GM) organisms is actively progressing worldwide, highlighting an increased need for improved safety management protocols. We sought to establish an environmental monitoring method, using real-time polymerase chain reaction (PCR) and propidium monoazide (PMA) treatment to develop a quantitative detection protocol for living GM microorganisms. We developed a duplex TaqMan quantitative PCR (qPCR) assay to simultaneously detect the selectable antibiotic gene, ampicillin (AmpR), and the single-copy Escherichia coli taxon-specific gene, D-1-deoxyxylulose 5-phosphate synthase (dxs), using a direct cell suspension culture. We identified viable engineered E. coli cells by performing qPCR on PMA-treated cells. The theoretical cell density (true copy numbers) calculated from mean quantification cycle (Cq) values of PMA-qPCR showed a bias of 7.71% from the colony-forming unit (CFU), which was within ±25% of the acceptance criteria of the European Network of GMO Laboratories (ENGL). PMA-qPCR to detect AmpR and dxs was highly sensitive and was able to detect target genes from a 10,000-fold (10^-4) diluted cell suspension, with a limit of detection at 95% confidence (LOD_95%) of 134 viable E. coli cells. Compared to DNA-based qPCR methods, the cell suspension direct PMA-qPCR analysis provides reliable results and is a quick and accurate method to monitor living GM E. coli cells that can potentially be released into the environment.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2003년도 식물바이오벤처 페스티발
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• pp.39-48
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• 2003

The ornamental industry encompasses cut flower, pot plant, turfgrass and nursery stock production and is an important part of the agricultural sector. As internationally traded commodities, cut flowers and plants are an integral part of the economy of a number of developing countries in South America, the Caribbean and Africa. Genetic modification (GM) is a tool with great potential to the ornamental horticulture industry. The rapid progress in our knowledge of plant molecular biology can accelerate the breeding ornamental plants using recombinant DNA technology techniques. Not only is there the possibility of creating new, novel products the driver of the industry but also the potential to develop varieties requiring less chemical and energy inputs. As an important non-food agricultural sector the use of genetically modified (GM) ornamental crops may also be ideal for the intensive farming necessary to generate pharmaceuticals and other useful products in GM plants. To date, there are only a few ornamental GM products in development and only one, a carnation genetically modified for flower colour, in the marketplace. International Flower Developments, a joint venture between Florigene Ltd. in Australia and Suntory Ltd.of Japan, developed the GM carnations. These flowers are currently on sale in USA, Japan and Australia. The research, development and commercialisation of these products are summarised. The long term prospects for ornamental GM products, like food crops, will be determined by the regulatory environment, and the acceptance of GM products in the marketplace. These critical factors will be analysed in the context of the current legislative environment, and likely public and industry opinion towards ornamental genetically modified organisms (GMO's).

• BMB Reports
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• 제57권1호
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• pp.50-59
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• 2024

The application of gene engineering in livestock is necessary for various reasons, such as increasing productivity and producing disease resistance and biomedicine models. Overall, gene engineering provides benefits to the agricultural and research aspects, and humans. In particular, productivity can be increased by producing livestock with enhanced growth and improved feed conversion efficiency. In addition, the application of the disease resistance models prevents the spread of infectious diseases, which reduces the need for treatment, such as the use of antibiotics; consequently, it promotes the overall health of the herd and reduces unexpected economic losses. The application of biomedicine could be a valuable tool for understanding specific livestock diseases and improving human welfare through the development and testing of new vaccines, research on human physiology, such as human metabolism or immune response, and research and development of xenotransplantation models. Gene engineering technology has been evolving, from random, time-consuming, and laborious methods to specific, time-saving, convenient, and stable methods. This paper reviews the overall trend of genetic engineering technologies development and their application for efficient production of genetically engineered livestock, and provides examples of technologies approved by the United States (US) Food and Drug Administration (FDA) for application in humans.

• 한국동물생명공학회지
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• 제38권4호
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• pp.247-253
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• 2023

Revolutionary advancements, such as the reduction in DNA sequencing costs and genome editing, have transformed biotechnology, fostering progress in manipulating biomolecules, engineering cells, and computational biology. Agriculture and food production have significantly benefited from tools like high-throughput microarrays, accelerating the selection of desired traits. Genetic engineering, especially utilizing genome editing, facilitates precise alterations in plants and animals, harnessing microbiomes and fostering lab-grown meat production to alleviate environmental pressures. The emergence of new biotechnologies, notably genome editing, underscores the necessity for regulatory frameworks governing LM (living modified) organisms. Global regulations overseeing genetically engineered or genome-edited (GE) organisms, encompassing animals, exhibit considerable diversity. Nonetheless, prevailing international regulatory trends typically exclude genomeedited plants and animals, employing novel biotechnological techniques, from GMO/ LMO classification if they lack foreign genes and originate through natural mutations or traditional breeding programs. This comprehensive review scrutinizes ongoing risk and safety assessment cases, such as genome-edited beef cattle and fish in the USA and Japan. Furthermore, it investigates the limitations of existing regulations related to genome editing in Korea and evaluates newly proposed legislation, offering insights into the future trajectory of regulatory frameworks.

• Journal of Ecology and Environment
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• 제38권4호
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• pp.437-442
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• 2015

Studies to estimate seed longevity and dormancy of creeping bentgrass (Agrostis stolonifera L.) were conducted from 2000 to 2005 at Corvallis and Hermiston, Oregon. Seeds from three transgenic glyphosate resistant creeping bentgrass lines, 48-10, 48-13, and ASR368, and one non-transgenic glyphosate susceptible line, SR1020, were used. Creeping bentgrass seeds were buried at 3, 18 and 31 cm in 2000 and removed 6, 12, 18, 24, and 51 months later. Soil type and climatic conditions were different at the two locations. At Corvallis, the soil was a Malabon silty clay loam, and the winters wet and mild. The soil at Hermiston was an Adkins fine sandy loam, and winters drier and colder. Seeds of all creeping bentgrass lines deteriorated faster at Corvallis than at Hermiston. The estimated half-lives of creeping bentgrass lines buried at Corvallis were 8.4 to 20.2 months, while those buried at Hermiston were 8.4 to 37.7 months. At both sites, seeds of the glyphosate resistant lines, 48-10 and 48-13, deteriorated faster than the susceptible line, SR1020. However, seed deterioration in the resistant line, ASR368, was slower than all other creeping bentgrass lines. Based on the germination test, exhumed intact seeds at Corvallis were more dormant than those at Hermiston. If buried, it could be expected that viable creeping bentgrass seeds will persist more than 4 years after the seeds are introduced to a site, but environmental conditions can influence both seed longevity and dormancy.

• 농촌지도와개발
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• 제10권1호
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• pp.115-128
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• 2003

국제연합 식량농업기구 (FAO)에 의하면 UR 협상과 WTO 체제 출범 이후 농산물 수출국가와 선진국에서의 소득균형은 일반적으로 향상되었으나 순식량수입국가나 개발도상국가에서는 소득균형이 더 악화되고 농업영역의 쇠퇴를 가져왔다. 더욱이 식량안전상황은 농산물의 급격한 수입으로 여전히 불안하고 농가판매의 지속적인 악화, 농촌과 도시간의 소득격차가 큰 것으로 나타나고 있다. 특히 인간의 건강과 환경에 유해한 GMO의 거래는 소비자의 식품안전과 잠재적 위해 때문에 적절한 조치를 필요로 하고 있다. 아시아 지역 개발도상국가들에서의 식량의 안정적 공급과 농촌 지역사회 개발의 핵심적 과제는 인적자원의 개발, 기술역량의 신장, 하부구조의 개선, 농업기술 이전의 효율화 등으로 요약될 수 있다. 이러한 상황에서 농업교육, 연구 그리고 지도, 특히 아시아의 주요작물 영역에 대한 더 많은 투자가 필요하며 많은 아시아국가들에서 우선적으로 요구되는 농촌청소년교육은 빈곤퇴치, 식량의 안정적 공급 및 식품의 안전 그리고 지속적이고 균형있는 농촌개발 등이다. 이를 위한 정부와 국민, 특히 젊고 열정적인 사람들이 함께 협력해야 빈곤퇴치와 식량의 안정적 공급 및 식품의 안전 등 농촌의 사회경제적 발전이 가능할 것이다. 진행되고 있는 WTO 체제 하에서의 아시아 지역 농촌사회 발전과 식량문제 해결을 위해서는 교역과 환경, 그리고 개발의 총체적 접근이 중요하며 식량의 안정적 공급을 위한 농업, 농촌의 하부구조가 열악한 개발도상국 소농에 대한 우선적 배려가 필수적이라 할 것이다. 특히, 농업분야 생산 유통 분야의 개선을 위한 농업교육, 농촌지도의 국가적 강조와 농촌 청소년 교육 훈련과 육성의 중요성에 대한 인식과 이에 따른 국가적, 국제적 협력이 필요할 것이다.

• Preventive Nutrition and Food Science
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• 제17권4호
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• pp.274-279
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• 2012

For the quantitative analysis of genetically modified (GM) maize in processed foods, primer sets and probes based on the 35S promoter (p35S), nopaline synthase terminator (tNOS), p35S-hsp70 intron, and zSSIIb gene encoding starch synthase II for intrinsic control were designed. Polymerase chain reaction (PCR) products (80~101 bp) were specifically amplified and the primer sets targeting the smaller regions (80 or 81 bp) were more sensitive than those targeting the larger regions (94 or 101 bp). Particularly, the primer set 35F1-R1 for p35S targeting 81 bp of sequence was even more sensitive than that targeting 101 bp of sequence by a 3-log scale. The target DNA fragments were also specifically amplified from all GM labeled food samples except for one item we tested when 35F1-R1 primer set was applied. A reference plasmid pGMmaize (3 kb) including the smaller PCR products for p35S, tNOS, p35S-hsp70 intron, and the zSSIIb gene was constructed for real-time PCR (RT-PCR). The linearity of standard curves was confirmed by using diluents ranging from $2{\times}10^1{\sim}10^5$ copies of pGMmaize and the $R^2$ values ranged from 0.999~1.000. In the RT-PCR, the detection limit using the novel primer/probe sets was 5 pg of genomic DNA from MON810 line indicating that the primer sets targeting the smaller regions (80 or 81 bp) could be used for highly sensitive detection of foreign DNA fragments from GM maize in processed foods.

• 한국해양바이오학회지
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• 제11권2호
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• pp.52-61
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• 2019

Over the past few decades, microalgae-based biotechnology conjugated with innovative CRISPR/Cas9-mediated genetic engineering has been attracted much attention for the cost-effective and eco-friendly value-added compounds production. However, the discharge of reproducible living modified organism (LMO) into environmental condition potentially causes serious problem in aquatic environment, and thus it is essential to assess potential environmental risk for human health. Accordingly, in this study, we monitored discharged genetically modified microalgae (GMM) near the research complex which is located in Daejeon, South Korea. After testing samples obtained from 6 points of near streams, several green-colored microalgal colonies were detected under hygromicin-containing agar plate. By identification of selection marker genes, the GMM was not detected from all the samples. For the lab-scale environmental risk assessment of GMM, acute toxicity test using rotifer Brachionus calcyflorus was performed by feeding GMM. After feeding, there was no significant difference in mortality between WT and transformant Chlamydomonas reinhardtii. According to further analysis of horizontal transfer of green fluorescence protein (GFP)-coding gene after 24 h of incubation in synthetic freshwater, we concluded that the GFP-expressed gene not transferred into predator. However, further risk assessments and construction of standard methods including prolonged toxicity test are required for the accurate ecological risk assessment.