• Journal of Applied Biological Chemistry
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• 제61권4호
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• pp.305-314
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• 2018

'New Breeding Techniques (NBTs)' have been one of hot issues, since their future will be affected profoundly by national as well as international regulatory landscapes. In this review, we compare characteristics of NBTs with conventional and genetic modification, and analyze genetically modified organism (GMO) regulatory systems in the context of possible regulation of NBTs. NBTs are very heterogeneous in terms of principles, methodologies, and final products. As Living Modified Organisms (LMO) is defined in the Cartagena Protocol on Biosafety (CPB) as an organism containing novel combination of genetic materials obtained by the use of modern biotechnology, CPB as well as other national legislations locate itself somewhere in the middle between product-based and process-based regulations. It is also noted that jurisdictions with regulatory systems more oriented to product-based one tend to be more productive and decide or may decide to exempt site-directed nucleases-1 from GMO regulation. In this context, Korean legislations are reviewed to clarify the commons and differences in GMO definitions. Act on Transboundary Movement of LMO Act, Food Sanitation Act and Agricultural and Fishery Products Quality Control Act are three major acts to regulate GMOs. It is noted that there are differences in the definition of LMO or GM food/products especially between the LMO Act and the Food Sanitation Act. Such differences may cause conflicts between Acts when policy-decision regarding the regulation of NBTs is made. Therefore, it is necessary to reorganize legislations before policies regarding the regulation of any techniques from biotechnology are made.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2006년도 추계학술대회
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• pp.87-94
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• 2006

Genetically modified (CM) crops with agricultural traits including herbicide resistance and insect tolerance have been commercialized. The safety testing strategies conducted for food and feed ingredients from GM crops differ from those applied to food ingredients in that they are conducted to demonstrate similarity between the CM food and the appropriate non-CM comparator rather than for quantitative risk assessment. However, there are similarities in the design and conduct of the safety assessment studies between these types of studies that should be readily recognized by toxicologists. The current presentation reviews some of the basic principles of safety assessment of typical dietary ingredients and compares and contrasts them with the testing strategies applied to CM foods and products obtained from them.

• Asian-Australasian Journal of Animal Sciences
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• 제29권9호
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• pp.1322-1330
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• 2016

This experiment was conducted to determine standardized ileal amino acid digestibility (SIAAD) of commonly used feed ingredients in poultry diets in Pakistan. These feed ingredients included corn, rice broken (RB), rice polishings (RP), wheat bran (WB), sunflower meal (SFM), cottonseed meal (CSM), guar meal (GM), soybean meal (SBM) from India and Argentine and fish meal (FM). The SIAAD of each ingredient was determined in triplicate using 21-days-old broilers. Day-old male broiler chicks (Hubbard${\times}$ Hubbard) were reared on corn-SBM based diet from 1 to 13 days and thereafter birds were fed experimental diets from day 14 to 21. Each diet was fed to 36 birds kept in six replicate cages, each cage had six birds. In cereals, the SIAAD of corn's amino acid (AA) (90.1%) was similar (p>0.05) to RB (89.0%). Isoleucine (97.8%) and lysine (96.9%) were highly digestible AA in corn and RB, respectively. Among cereal-by products, WB's SIAAD (76.9%) was same (p>0.05) as RP (71.9%). Arginine from WB (82.5%) and RP (83.2%) was highly digestible. However, threonine in WB (72.7%) and leucine in RP (69.6%) were the lowest digestible AAs. In plant protein meals, AAs from Argentine-SBM (85.1%) and Indian-SBM (83.4%) had higher (p<0.5) SIAAD than other protein meals. However, SIAAD of SFM (77.1%) and CSM (71.7%) was intermediate while GM (60.3%) exhibited the lowest (p<0.05) SIAAD among all ingredients. Arginine from GM (76.9%), CSM (85.8%), SBM-India (89.5%) and SBM-Argentine (91.5%) was highly digestible from indispensable AAs. In SFM, methionine (91.4%) SIAAD was the greatest. The average SIAAD of FM was 77.6%. Alanine from FM had the highest (84.0%) but cysteine (62.8%) had the lowest SIAAD. In conclusion, cereals i.e. corn and RB had higher (p<0.05) SIAAD of the cereals by-products. The SIAAD of RP and WB was same (p>0.05). The SBM from plant protein meals had higher (p<0.05) SIAAD than other studied feed ingredients. However, the GM had the lowest (p<0.05) SIAAD among protein meals.

• Molecules and Cells
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• 제23권3호
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• pp.280-286
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• 2007

L-asparaginase (EC 3.5.1.1) catalyzes the hydrolysis of the amide group of L-asparagine, releasing aspartate and $NH_4{^+}$. We isolated a low temperature-inducible cDNA sequence encoding L-asparaginase from soybean leaves. The full-length L-asparaginase cDNA, designated GmASP1, contains an open reading frame of 1,258 bp coding for a protein of 326 amino acids. Genomic DNA blotting and fluorescence in situ hybridization showed that the soybean genome has two copies of GmASP1. GmASP1 mRNA was induced by low temperature, ABA and NaCl, but not by heat shock or drought stress. E. coli cells expressing recombinant GmASP1 had 3-fold increased L-asparaginase activity. A possible function of L-asparaginase in the early response to low temperature stress is discussed.

• 한국식품영양과학회지
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• 제42권10호
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• pp.1629-1637
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• 2013

Lipoxygenase(LOX)가 결핍된 non-GM콩인 개척#1(검정콩, LOX2,3-free), 개척#2(황색콩, LOX2,3-free) 및 진양콩(황색콩, LOX1,2,3-free)의 이화학적 특성과 두부 수율을 LOX가 존재하는 태광콩(황색콩, LOX1,2,3-present)과 비교하였다. 콩의 길이 및 백립중은 태광콩에 비해 개척#1이 유의적인 차이로 컸으며, 황색콩에서는 LOX가 결핍된 품종이 태광콩보다 크기 및 중량이 다소 작았다. 콩을 12시간 동안 수침한 후 콩의 중량 증가율은 217.07~226.55%로 개척#2가 유의적으로 높았다. 전체적인 색차(${\Delta}E$)는 개척#1이 유의적으로 높았으며, 3종의 황색콩은 시료 간에 비슷한 경향이었다. 조지방 함량은 태광콩이 17.30%로 유의적으로 높았으며, LOX가 결핍된 콩은 13.71~14.66%로 시료 간에 유의차가 없었다. 조단백질 함량은 개척#1(34.79%)이 타시료(32.93~33.47%)에 비해 유의적으로 높았다. 구성아미노산 함량은 모든 시료에서 glutamic acid의 함량이 가장 높았으며, 총량은 개척#1이 32.42 g/100 g으로 가장 많았고 개척#2와 태광콩은 비슷한 함량이었다. 이소플라본 함량은 LOX가 결핍된 콩이 태광콩에 비해 더 많았으며, 특히 개척#1(1672.54 ${\mu}g/g$)에서 가장 높았다. 콩의 총 페놀 함량은 개척#1이 276.91 mg/100 g으로 가장 높았으며, 플라보노이드 함량은 개척#1(26.93 mg/100 g)이 타 시료에 비해 2.6~2.8배 정도 높았다. DPPH, ABTS 라디칼 소거활성 및 환원력은 개척#1이 타 시료에 비해 높았다. Non-GM콩으로 제조한 두부의 수율은 개척#2에서 가장 높았다. 황색콩인 개척#2는 이화학적 특성 및 항산화 활성 측면에서 태광콩과 유사하였고 두부 수율이 높아 두부 제조에 적합한 품종인 것으로 사료된다.

• 한국수산과학회지
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• 제30권5호
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• pp.834-841
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• 1997

항균성 물질인 박테리오신을 생산하는 젖산균을 시판유제품에서 분리하여 그의 형태적, 생리 생화학적 특성을 실험한 결과, Lactobacillus sp. 와 유사한 성질을 나타내어 이를 Lactobacillus sp. GM7311로 명명하여 본 실험의 공시 균주로 사용하였다. 본 균이 생산한 박테리오신은 Gram 양성과 음성균에 고른 항균력을 나타내었고 그중 Proteus mirabilis에 대해 강한 항균력을 나타내어 이를 항균 지표세균으로 선정하여 실험하였다. Lactobacillus sp. GM7311은 초기 pH를 6.0으로 조정한 MRS broth에서 $37^{\circ}C$ 배양한 경우가 박테리오신 생산 최적조건이었고, 이 조건에서 박테리오신은 대수기 말기부터 정상기 초기에 걸쳐 최대로 생산되었고 그 이후에는 약간씩 감소하는 경향을 나타내었다. 생산된 박테리오신의 활성은 산성측 $(pH 2.0\~5.0)$에서는 안정하였으나, pH 6.0 이상에서는 활성이 급격히 감소되는 경향을 나타내었다. 열에 대한 안정성은, pH 5.0의 sodium acetate buffer에 녹인 박테리오신은 $100^{\circ}C$에서 60분 가열에도 활성 변화가 없었으나, 0.02N-HCl(pH 2.3)에 녹인 박테리오신은 $60\~121^{\circ}C$의 가열로 초기 15분 동안 활성이 $40\~50\%$ 정도 감소하였고, 그 이후 $100^{\circ}C$ 이하의 온도에서는 가열시간의 증가에도 활성 변화는 거의 나타나지 않았다. 그리고 유기용매에 대해서는 대체로 불안정하여 acetone, ethanol, iso-butanol, 그리고 ethyl ether을 박테리오신과 동량 혼합하여 $4^{\circ}C$에서 2시간 방치한 결과 초기 활성의 약 $40\%$가 감소하였다.

• Journal of Microbiology and Biotechnology
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• 제16권1호
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• pp.25-31
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• 2006

Changes of CP4 5-enolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS) in the glyphosate-tolerant Roundup Ready soybean were examined using purified CP4 EPSPS produced in cloned Escherichia coli as a control. CP4 EPSPS in genetically modified soybean was detected by twodimensional gel electrophoresis (2-DE) and identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and electrospray ionization tandem mass spectrometry (ESI-MS/MS) with databases. CP4 EPSPS in soybean products was resolved on 2-DE by first isoelectric focusing (IEF) based on its characteristic pI of 5.1, followed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) based on its molecular mass of 47.5 kDa. We quantified various percentages of soybean CP4 EPSPS. The quantitative analysis was performed using a 2D software program on artificial gels with spots varying in Gaussian volumes. These results suggested that 2-DE image analysis could be used for quantitative detection of GM soybean, unlike Western blotting.

• Journal of Nutrition and Health
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• 제15권3호
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• pp.171-180
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• 1982

The nutritional survey and blood sampling was conducted from May 23 to 27, 1971, in order to investigate the state of the nutritional intake in relation to blood contents of rural women. This survey covered a total of 47 women residing in Youngam-gun, Cholla-namdo, Korea. The daily diet of these subjects was observed by 24-hour recall method and weighing method for 3 days and hemoglobin (Hb), hematocrit(Hct), mean corpuscular hemoglobin concentration (MCHC), serum protein, serum albumin, A/G ratio, serum iron, total iron binding capacity (TIBC), transferrin saturation (TS), serum cholesterol and blood specific gravity (SG) were measured. The results were summarized as follows ; The rate of intake of vegetable foods was high, especially cereals, and that of animal foods was low, especially eggs and milk and milk products. The daily intake of niacin was sufficient, and the intakes of energy and iron were also tend to be adequate. On the contrary, the intakes of vitamin A, $B_2$ and C, calcium and protein were insufficient. The mean red blood cell number was $410.8{\pm}26.4{\times}10^{4}/mm^{3},$ the mean values of Hb, Hct and MCHC were $12.5{\pm}0.9gm%$, $37.5{\pm}2.4%$ and $33.2{\pm}0.5%$ thus the anemic prevalences were 21.3%, 12.8% and 93.6% comparing with the anemic criteria of WHO. The levels of serum protein and serum albumin were $7.1{\pm}0.4gm\;%$ and $4.3{\pm}0.3gm\;%$so the A/G ratio was indicated $1.6{\pm}0.2.$. The levels of serum iron and TIBC were $91.9{\pm}27.5{\mu}g/100ml and $297.5{\pm}31.4{\mu}g/100ml,$ so TS was calculated $31.2{\pm}9.7\;%$. The mean value of SG was $1.044{\pm}0.002,$ and the level of serum cholesterol was $164.3{\pm}25.4mg/dl$. In the relation between each of bloood content, Hb showed positive correlation not only with Hct but also protein at the level of significance of 1 per cent and 5 per cent, and protein also showed a positive correlation with serum cholesterol at the 1 per cent level of significance. In the relation between the nutrient intake and blood contents, vitamin C intake showed a positive correlation with Hb, serum protein and serum iron at the level of significance of 1 per cent, iron intake also showed a positive correlation with serum iron and serum cholesterol at the same significant level, and protein intake showed a positive correlation with serum iron at the at the level of significance of 5 per cent.

• 한국축산식품학회지
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• 제40권2호
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• pp.209-220
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• 2020

Milk fat globule membrane (MFGM) is a lipid carrier in mammals including humans that consists mainly of polar lipids, like phospholipids and glycolipids. In this study, a process to enrich polar lipids in commercial butter and whey powder, including polar lipids of MFGM, was developed. WPC (whey protein concentrate) 60 was selected as the most suitable raw material based on the yield, phospholipid, protein, and lactose content of the polar lipid fraction obtained by ethanol extraction of two WPC (WPC60 and WPC70) and two buttermilk (A and B). After fractionation under optimum conditions, the polar-lipid enriched fraction from WPC60 contained 38.56% phospholipids. The content of glycolipids, cerebroside, lactosylceramide, ganglioside GM3, ganglioside GD3, was 0.97%, 0.55%, 0.09%, and 0.14%, respectively. Rancimat results showed that the oxidation stability of fish oil increased with an increase in the polar-lipid fraction by more than 30 times. In addition, the secretion of IL-6 and TNF-α decreased in a concentration-dependent manner after treatment of RAW 264.7 cells with 0.1 to 100 ppm of the polar lipid fraction. In this study, polar lipid concentrates with antioxidant and anti-inflammatory activity, were prepared from milk processing by-products. The MFGM polar lipid concentrates made from by-products are not only additives for infants, but are also likely to be used as antioxidants in cooking oils and as active ingredients for functional foods.

• 가정과삶의질연구
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• 제20권4호
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• pp.89-102
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• 2002

Genetically modified foods may be defined as the foods deemed as safe by current technology among the many kinds of agricultural and stockbreeding products that are now under research and development using contemporary gene-modification techniques. This study examines hotly debated arguments, both for and against genetically modified- foods, in various countries. This study also investigates consumers'rights and responsibilities. Countries that are developing and exporting genetically modified organisms(GMO) have maintained that GMO can help produce more crops while reducing labor and other production-related costs, and that the genetically modified foods signify ″the second green revolution,″ which will solve future food and environmental problems by strengthening specific nutritive substances and extending shelf-life. But consumer groups, environmental organizations. and food-importing countries are more cautious about importing and consuming those foods because the potential dangers of GMO to human bodies and the environment have not been tested thoroughly yet. South Korea, following suit with others such as EU, Japan, Australia, and New Zealand, introduced a law on 'Labeling of Genetically Modified Foods', which went in effect in March, 2001, on the basis of customers'rights to make informed choices. The law takes the ″precautionary principle″ into consideration, rather than stopping at insuring ″substantial equivalence″ in developing and consuming GM foods. The actual impact of the law will depend on the level of citizens'Participation more than on the government's willingness to carry out the law. So far the level of Korean consumers'consciousness about genetically modified foods is very low. Therefore, it is hard to expect consumers to exercise their ″rights not to buy″ foods that are potentially unsafe. The Korean government must devise an effective plan to inform and educate the people about the labeling of genetically modified foods.