• Preventive Nutrition and Food Science
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• v.8 no.1
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• pp.61-65
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• 2003

This study was investigated to identify the regulatory mechanism of ACC gene expression by hormones and nutrition. The fragment of ACC promoter I (PI) -220 bp region was recombined to pGL3-Basic vector with luciferase as a reporter gene. The primary hepatocyte from the rat was used to investigate the regulation of ACC PI activity. ACC PI (-220 bp)/luciferase chimeric plasmid was transfected into primary rat hepatocyte by using lipofectin. ACC PI activity was shown by measuring luciferase activity. The addition of insulin, dexamethasone, and triiodothyronine to the culture medium increased the activity of ACC PI by 2.5-, 2.3- and 1.8-fold, respectively. In the presence of 1 $\mu$M dexamethasone, the effects of insulin was amplified about 1.2-fold showing the additional effects of dexamethasone. Moreover the activity of luciferase was increased by insulin, dexamethasone, and triiodothyronine treatment approximately 4-fold. These results indicated that insulin, dexamethasone and thyroid hormone coordinately regulate ACC gene expression via regulation of promoter I activity. On the -220 to ＋21 region of ACC PI, the addition of the glucose to the culture medium increased the activity of ACC PI. With 25 mM glucose, luciferase activity increased by 7-fold. On the other hand, on the -220 bp region, ACC PI activity was not changed by polyunsaturated fatty acids. Therefore, it can be postulated that there are response elements for insulin, triiodothyronine, dexamethasone, and glucose, but not PUFAs on the -220 bp region of ACC PI.

• Journal of Ocean Engineering and Technology
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• v.27 no.6
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• pp.43-55
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• 2013

In general, huge caissons for breakwaters have been constructed on land or a floating dock. In the case of the construction on a floating dock, a 4 step installation procedure is involved: i) construction on a floating dock, ii) transportation by the floating dock to an area near the target sea, iii) launching from the floating dock, and iv) transference by tug-boats to the installation site. It is especially important to pay attention to the dynamic stability of the floating dock against the conditions in the sea during steps i) and iii). In this paper, the static and dynamic stabilities of a caisson on a floating dock are evaluated based on IMO rules during the construction and launching of the caisson on a floating dock by using independent commercial S/Ws such as NAPA, WAMIT, and CHARM3D.

• Journal of Pharmacopuncture
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• v.4 no.1
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• pp.123-124
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• 2001

Purpose. Free radicals are implicated in the pathophysiology of aging, ischemic injury and neurodegenerative disorders. To deform]no whether Hominis Placenta extract prevents $H_2O_2$-induced apoptosis, we have performed morphological and biochemical analyses for the detection of apoptotic phenomena in the pineal tumor cell line $PGT-{\beta}$ We have also peformed cytochemical and immunocytochemical analyses for the detection of changes in nitric oxide synthase (NOS) activity and estimated the expression . of apoptotic genes using reverse transcription-polymerase chain reaction (RT-PCR) Methods. $PGT-{\beta}\;cells$ were pretreated with Hominis Placenta extracts $(0,\;10^{-2}\;{\mu}g/ml)$ for 2 hours and then exposed to $H_2O_2\;(0,\;50\;{\mu}M)$ for 3 hours. Appearance of apoptotic characteristics were monitored using 4, 6-diamidino-2-phenylindole dihydrochloride (DAPI) staining assay, terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end labeling (TUNEL) assay and flow cytometric analysis. NOS activity was measured by NADPH-diaphorase cytochemistry. Expression of inducible NOS (iNOS) and nuclear factor kappa B (NF k B) was assessed via immunocytochemistry. The expression of apoptotic genes was examined by RT-PCR. Results. After 3 flours of exposure to $H_2O_2$, it was shown that $PGT-{\beta}\;cells$ treated with $H_2O_2(50\;{\mu}M)$ exhibit classical apoptotic features and increases in NOS activity and caspase-3 expression. Treatment with Hominis Placenta extract resulted in a reduced occurrence of apoptotic features. DAPI staining, TUNEL and flow cytometric assays revealed decreases in the occurrence of nuclear fragmentation and in the sub-Gl fraction in the $PGT-{\beta}\;cells$ treated with Hominis Placenta extract. Cells treated with Hominis Placenta extract also showed lower activity of NADPH-diaphorase and immunoreactivities of both iNOS and NF k B than those of $H_2O_2$-treated cells which were not treated with Hominis Placenta extract. By RT-PCR, it was shown that the level of caspase-3 mRNA was derreased In the cells treated with Hominis Placenta . extract. Conclusions. This study shows that Hominis Placenta extract prevents $H_2O_2$-induced apoptosis in $PGT-{\beta}\;cells$; inhibitions of iNOS and caspnse-3 are possible mechanisms of the protection against apoptosis.

• Journal of Environmental Science International
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• v.18 no.7
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• pp.747-754
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• 2009

In this study, which aims to estimate the volume of greenhouse gas emitted by road transportation vehicles in Changwon City, the emission rate was calculated on the basis of the actual traffic volume measured at major crossroads and compared with the results obtained from the methodology used to estimate the greenhouse gas emissions of road transportation provided in the IPCC 2006 GL guidelines (Tier 1, Tier 3). Analysis of the results of the comparison showed that the Tier 1 methodology, which was applied in the estimation of the rate of greenhouse gas emissions, carries a high probability of underestimation, while the Tier 3 methodology carries a relatively high probability of overestimation. Therefore, when considering the assignment of permissible rates of emission to local governments, the application of the methodology, i.e. whether one uses Tier 1 or Tier 3, may result in a large difference in the rate of allowable emissions. It is suggested that a method based on the actual volume of traffic would be the most reasonable one with regard to the development of a realistic plan.

• Journal of Advanced Marine Engineering and Technology
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• v.30 no.1
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• pp.93-101
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• 2006

This research makes a new attempt to apply the activated seawater by electrolysis in the development of two-stage wet scrubber system to control the exhaust gas of large marine diesel engines. First, with using only seawater that is naturally alkaline (pH typically around 8.1). the $SO_2\;and\;SO_3$ are absorbed by relatively high solubility compared to other components of exhaust pollutants, and PM (Particulate Matter) is removed through direct contact with sprayed seawater droplets. Besides, the electrolyzed alkaline seawater by electrolysis, which contains mainly NaOH together with alkali metal ions $(i.e.\;Na^+,\;Mg^{2+},\;Ca^{2+})$, is used as the absorption medium of NOx and $CO_2$. Especially, to increase NOx absorption rate into the alkaline seawater. nitric oxide (NO) is adequately oxidized to nitrogen dioxide $(NO_2)$ in the acidic seawater, which means both volume fractions are adjusted to identical proportion. The results found that the strong acidic seawater was a valid oxidizer from NO to $NO_2$ and the strong alkaline seawater was effective in $CO_2$ absorption In the scrubber test, the SOx reduction of nearly $100\%$ could be achieved and also led to a sufficientPM reduction. Hence, the author believes that applying seawater and its electrolyte would bring the marine air pollution control system to an economical measure. Additionally it is well known that NOx and SOx concentration has a considerable influence on the $N_2O$ emission of green house gas. Although the $N_2O$ concentration exhausted from diesel engines is not as high, the green house gas effect is around 300 times greater than an equivalent volume of $CO_2$. Therefore, we investigated the $N_2O$ removal efficiency with using the electrolyzed seawater too. Finally this research would also plan to treat the effluent by applying electro-dialysis and electro-flotation technique s in the future.

• Journal of the Korean Society of Radiology
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• v.14 no.5
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• pp.529-535
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• 2020

Geant4 is compatible with the Windows operating system in C++ language use, enabling interface functions that link DICOM or software. It was simulated to address the basic structure of the simulation using Geant4/Gate code and to specifically verify the density composition and lung cancer process in the human phantom. It was visualized using the Gate Graphic System, i.e. openGL, Ray Tracer: Ray Tracing by Geant4 Tracing, and using Geant4/Gate code, lung cancer is modeled in the human phantom area in 3D, 4D to verify the simulation progress. Therefore, as a large number of new functions are added to the Gate Code, it is easy to implement accurate human structure and moving organs.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.40 no.7
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• pp.616-622
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• 2012

In this paper, we examined the problems of the flare operation of tactic maneuvering flight of fighter aircraft with 150GL center external fuel tank and proposed the possible solution of it's operation. The damage scope of horizontal fin of fuel tank and flare trajectory when the flare ejected from the maneuvering aircraft were analyzed by the wind tunnel test and the numerical analysis. We investigated the two different option to avoid the damage of fin; i) the adjustment of flare dispenser angle and ii) the change of horizontal fin's shape. For the considering of practical operation of present system, we chose the second option. We estimated the drop safety of external fuel tank with redesigned fin by the wind tunnel experiments.

• Archives of Pharmacal Research
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• v.24 no.6
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• pp.557-563
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• 2001

Oral tolerance is thought to play a role in preventing allergic responses and immune-mediated diseases. An improved mouse model of the oral tolerance to Japanese cedar pollen (JCP) as antigen was developed in order to detect induction of the tolerance, and the immunological characteristics of this model were also elucidated. Oral tolerance was induced by C3H/ HeN mice given an oral administration of 10 mg JCP 7 days before immunization with an i.p. injection of 0.1 mg JCP in complete Freunds adjuvant (CFA). The effects of oral JCP on systemic immunity were assessed by enzyme-linked immunosorbent assay (ELISA) of immunoglobulin (Ig) levels in serum collected on day 7 or 14 after immunization. Oral tolerance to JCP was adequately induced on day 7 after immunization and was more effective in C3H/HeN mice than in BALB/c mice. The tolerance was primarily concerned with the decreased serum levels of antigen-specific IgG. In these mice, oral administration of JCP also suppressed various immune responses to the antigen including delayed-type hypersensitivity (DTH), total Igl level and anti-JCP IgGl level. The suppression of these immune responses by the oral antigen was associated with a significant reduction in interleukin-4 (IL-4) production. These findings therefore indicate that this C3H/HeN mice model has potential use in detecting the induction of oral tolerance by JCP and suggest that this tolerance model may be effective in the treatment and prevention of allergic responses caused by the antigen.

• Journal of the Korea Convergence Society
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• v.12 no.2
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• pp.193-200
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• 2021

As the degree of environmental pollution caused by the use of fossil fuels intensifies, many countries continue to invest in the development of alternative energy. PEMFC, one of the alternative energies, consists of four main components: bipolor plate, electrolyte, gas diffusion layer, and electrode. Among them, bipolor plate, the gas diffusion layer, and electrode are generally manufactured using carbon materials such as carbon black and carbon fiber. These carbon materials are expensive in manufacturing process or have disadvantages such as corrosion, and research is being conducted in many fields to improve this. This paper collects several research results conducted to improve the shortcomings of these three components and examines the trends of PEMFC by grasping what problems have been and how they have improved.

• Food Science of Animal Resources
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• v.43 no.5
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• pp.914-937
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• 2023

The objective of this study was to establish a multi-residue quantitative method for the analysis of anthelmintic and antiprotozoal drugs in various livestock products (beef, pork, and chicken) using ultra-high-performance liquid chromatography-tandem mass spectrometry. Each compound performed validation at three different levels i.e., 0.5, 1, and 2× the maximum residue limit according to the CODEX guidelines (CAC/GL 71-2009). This study was conducted according to the modified quick, easy, cheap, effective, rugged, and safe procedure. The matrix-matched calibrations gave correlation coefficients >0.98, and the obtained recoveries were in the range of 60.2%-119.9%, with coefficients of variation ≤32.0%. Furthermore, the detection and quantification limits of the method were in the ranges of 0.03-3.2 and 0.1-9.7 ㎍/kg, respectively. Moreover, a survey of residual anthelmintic and antiprotozoal drugs was also carried out in 30 samples of beef, pork, and chicken collected in Korea. Toltrazuril sulfone was detected in all three samples. Thus, our results indicated that the developed method is suitable for determining the anthelmintic and antiprotozoal drug contents in livestock products.