• Journal of Microbiology and Biotechnology
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• v.28 no.3
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• pp.498-502
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• 2018

Molecular imaging is a powerful method for tracking various infectious disease-causing pathogens in host organisms. Currently, a dual molecular imaging method that can provide temporal and spatial information on infected hosts at the organism, organ, tissue, and cellular levels simultaneously has not been reported for Burkholderia pseudomallei, a high-risk pathogen that causes melioidosis. In this study, we have established an experimental method that provides spatiotemporal information on infected hosts using luminescent and fluorescent dual-labeled B. pseudomallei. Using this method, we visualized B. pseudomallei infection at the organism, organ, and tissue levels in a BALB/c mouse model by detecting its luminescence and fluorescence. The infection of B. pseudomallei at the cellular level was also visualized by its emitted fluorescence in infected macrophage cells. This method could be an extremely useful and applicable tool to study the pathogenesis of B. pseudomallei-related infectious diseases.

• Genomics & Informatics
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• v.6 no.2
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• pp.91-94
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• 2008

Single nucleotide polymorphisms (SNPs) are the most abundant form of human genetic variation and are a resource for mapping complex genetic traits. A genome is covered by millions of these markers, and researchers are able to compare which SNPs predominate in people who have a certain disease. The International HapMap Project, launched in October, 2002, motivated us to start the Korean HapMap Project in order to support Korean HapMap infrastructure development and to accelerate the finding of genes that affect health, disease, and individual responses to medications and environmental factors. A Korean SNP and haplotype database system was developed through the Korean HapMap Project to provide Korean researchers with useful data-mining information about disease-associated biomarkers for studies on complex diseases, such as diabetes, cancer, and stroke. Also, we have developed a series of software programs for association studies as well as the comparison and analysis of Korean HapMap data with other populations, such as European, Chinese, Japanese, and African populations. The developed software includes HapMapSNPAnalyzer, SNPflank, HWE Test, FESD, D2GSNP, SNP@Domain, KMSD, KFOD, KFRG, and SNP@WEB. We developed a disease-related SNP retrieval system, in which OMIM, GeneCards, and MeSH information were integrated and analyzed for medical research scientists. The kHapMap Browser system that we developed and integrated provides haplotype retrieval and comparative study tools of human ethnicities for comprehensive disease association studies (http://www.khapmap.org). It is expected that researchers may be able to retrieve useful information from the kHapMap Browser to find useful biomarkers and genes in complex disease association studies and use these biomarkers and genes to study and develop new drugs for personalized medicine.

• Journal of Korean Medical classics
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• v.23 no.3
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• pp.23-48
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• 2010

Onbyeong(溫病) is called an acute epidemic febrile disease caused by warm pathogen, a major symptom of Onbyeong is high fever. Doctrine of Onbyeong is a study of an occurrence, progress and treatments of an acute epidemic febrile disease. Doctrine of Onbyeong is valid in the Cheong Dynasty at China. Now, a theory of doctrine of Onbyeong at China and Korea is being applied in not only an acute febrile disease but also many other lifestyle diseases. Onbyeongjobyeon is a book written by Oguktong(吳鞠通). Oguktong was influenced by Jangjung-gyeong(張仲景) "Sanghanron(傷寒論)". Oguktong had organized Seopcheonsa(葉天士)'s medical thoughts and Oguktong's medical experiences. A Samcho(三焦) deteriorated case is divided into three groups - Upper, Middle and Lower-energizer - that is discussed of a vertical progress of Onbyeong. And a Wigiyeonghyeol(衛氣營血) deteriorated case is divided into four groups - Wi, Gi, Yeong and Hyeol - that is discussed of a horizontal progress of Onbyeong. In Korean medicine, there are four types of diagnosis which are watching, listening, asking and taking. Informations, got by four types diagnosis are synthesized and classified for medical treatments. A pulse diagnosis belongs to a method by taking a wrist among four diagnosis. A Korean Medicine doctor makes a conclusion of cause, region and condition of disease by taking a pulse. Because all organs in human body are connected by a meridian system. organs conditions are reflected in a meridian system. So by taking a pulse, a progress and a prognosis of disease is diagnosed In this thesis, by taking a pulse on "Onbyeongjobyeon(溫病條辨)", a location and a feature of disease's cause with weakness and strength of a vital force are examined, and a character of a pulse diagnosis of Onbyeong is examined.

• Korean Journal of Veterinary Service
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• v.39 no.2
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• pp.87-92
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• 2016

Porcine edema disease (ED) is a communicable disease of pigs caused by infection with Shiga toxin (Stx)-producing Escherichia coli (STEC) which expresses F18 fimbriae and/or Stx type 2e (Stx2e). While STEC causes a severe illness including hemorrhagic colitis and hemolytic-uremic syndrome in humans, it induces damage to the vascular endothelium, which results in edema, hemorrhage, and microthrombosis, leading in high mortality in pigs. In the present study, we cultured Stx2e-producing E. coli field isolates from conventional pig farms that experienced sudden deaths previously with symptoms similar to porcine edema disease, which were further investigated with Shiga toxin profiles. A total of 43 strains were identified from the collected samples by F18 or Stx2e specific PCR. Based on the PCR, 42 isolates out of 43 isolates were proved to carry one of F18 or Stx2e genes and 14 isolates to carry both F18 and Stx2e genes. All of the 30 isolates that harbored Stx2e gene induced the cytopathic effect (CPE) in vero cells and especially, the isolate 150229 produced the highest level of Shiga toxin. Therefore, we identified the virulence genes (F18 and Stx2e) and demonstrated Shiga toxin-producing abilities from porcine edema disease causing E. coli filed isolates. These results suggested that one of the isolates could be a vaccine antigen candidate against STEC through further investigating to elicit an immune response.

• Genomics & Informatics
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• v.7 no.1
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• pp.32-37
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• 2009

The quality assurance (QA) is of utmost importance in biobanks when archived biomaterials are distributed to biomedical researchers. For sample authentication and cross-contamination detection, the two fundamental elements of QA, STR genotyping is usually utilized. However, the incorporated number of STR markers is highly redundant for biobanking purposes, resulting in time and cost inefficiency. An index to measure the cross-contamination detection capability of an STR marker, the mixture probability (MP), was developed. MP as well as other forensic parameters for STR markers was validated using STR genotyping data on 2328 normal Koreans with the commercial AmpFlSTR kit. For Koreans, 7 STR marker (D2S1338, FGA, D18S51, D8S1179, D13S317, D21S11, vWA) set was sufficient to provide discrimination power of ${\sim}10^{-10}$ and cross-contamination detection probability of ${sim}1$. Interestingly, similar marker sets were obtained from African Americans, Caucasian Americans, and Hispanic Americans under the same level of discrimination power. Only a small subset of commonly used STR markers is sufficient for QA purposes in biobanks. A procedure for selecting optimal STR markers is outlined using STR genotyping results from normal Korean population.

• Journal of Korean Medical classics
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• v.21 no.1
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• pp.43-62
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• 2008

After parturition, woman in childbed's health can grow worse. Because when woman in childbed gives birth, Genital organ can be damaged, and lost a lot of blood, and also consume a lot of physical strengths. So, after parturition, woman in childbed is easy to fall in disease if do not take care in health specially. So after parturition, woman in childbed should be careful in stress, sleep, food etc. This thesis described Postpartum Spasm[痙病], Oppressive Feeling and Dizziness[鬱冒], Constipation[便秘], Abdominal Pain[腹痛], Diarrhea[下利], Annoyance[煩亂] and Vomitting Sickness[嘔逆]. Postpartum Spasm[痙病]'s major symptom is rigided body. This disease corresponds to Tetanus in modem medicine, and bacterial infection is assumed for cause. By Oriental medicine, after parturition, when blood is lacking, if patient takes wind, this disease breaks out. Oppressive Feeling and Dizziness[鬱冒]'s major symptom is dizzy head and gloomy eye. After parturition, when blood is lacking, if patient sweats too much and take cold, this disease breaks out. After parturition, if patient consumes a lot of resins of human body, Constipation[使秘] breaks out. There are four causes to Abdominal pain[腹痛]. First, due to deficiency and cold treats by Danggwisaenggangyangyuktang(當歸牛薑羊肉湯). Second, due to stagnation of Gi(氣) treats by Jisiljakyaksan(枳實芍藥散). Third, due to blood stasis treats by Ha-eohyeoltang(下瘀血湯). Fourth, due to lochiorrhea treats by Daeseunggitang(大承氣湯). After parturition, Annoyance[煩亂] and Vomitting Sickness[嘔逆] due to fever of difficiency treat by Jukpidaehwan(竹皮大丸). After parturition, Febrile diarrhea[熱性下利] treats by Baekduonggagamchoagyotang(白頭翁加甘草阿膠湯). This disease is similar with present Bacillary Dysentery. After parturition, Exteroceptive disease due to taking wind treats by Yangdaltang. Due to wind and fever treats by Jukyeoptang(竹葉湯).

• The Journal of Internal Korean Medicine
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• v.41 no.6
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• pp.993-1014
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• 2020

Objective: Parkinson's disease (PD) is the second most common neurodegenerative disease after Alzheimer's disease. Antioxidant stress and inflammatory reactions are important causes of neurodegenerative diseases and are major causes of PD. Many animal experiments have been aimed at treating PD using the antioxidant effects of various traditional medicines and dietary supplements. This review reports the research investigating the antioxidant effects of herbs in in vivo PD models. Methods: The study consisted of a database search for articles related to PD and herbal treatments using the OASIS, NDSL, KTKP, Korean KISS, PubMed, Science Direct, CNKI, Wanfang, and J-STAGE databases. The search period was limited from the start of the search engine application to November 14, 2019. Studies were selected to confirm the antioxidant effects of herbal medicines in an in vivo PD model. Results: Eighty-two studies were summarized for plant species, extracts (or compounds), animal models, neurotoxins, and functional results. The most frequently used herbal materials were Bacopa monnieri, Camellia sinensis, Centella asiatica, and Withania somnifera. MPTP and 6-OHDA were the most commonly used neurotoxins for inducing PD. Most studies confirmed an increased expression and activation of antioxidant enzymes and a decrease in oxidative stress. Herbal materials showed their antioxidant effects regardless of the order of treatment and confirmed their possible use as treatments for the prevention and treatment of neurodegeneration. Conclusion: Many herbal medicines have antioxidant effects and are likely to be effective in delaying neurodegenerative damage by inhibiting or reducing oxidative stress by expression of antioxidant enzymes.

• Pediatric Infection and Vaccine
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• v.27 no.3
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• pp.184-189
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• 2020

Lyme disease is a common vector-borne disease caused by Borrelia burgdorferi. Erythema migrans represents the most common manifestation during the early phase of this disease; however, systemic manifestations involving the nervous system, joints, or heart are known to occur. We report a case of Lyme disease accompanied by cardiac complications in a 13-year-old Korean male adolescent. The patient developed annular erythematous lesions on his lower extremities after a field trip during his visit to Connecticut, USA, for a boarding school camp, and his skin lesions were consistent with erythema migrans. Indirect immunofluorescence assay and Western blot analysis for Lyme immunoglobulin M showed positive results. Electrocardiography revealed a first-degree atrioventricular block, and he was diagnosed with Lyme carditis and received a 4-week course of oral doxycycline. Follow-up electrocardiography performed a week later revealed normal findings, and the patient showed an uneventful recovery. Lyme carditis often presents as an asymptomatic heart block that can progress to an advanced heart block; however, this condition is reversible with appropriate antibiotic treatment.

• Korean Journal of Head & Neck Oncology
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• v.31 no.2
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• pp.36-38
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• 2015

Small cell carcinoma is mainly found in the lungs and extrapulmonary origins of small cell carcinoma in GI tract, head and neck, genitourinary system consists only 4% of the tumor. Thus, small cell carcinoma of the parotid gland is an extremely rare disease. Extrapulmonary small cell carcinoma is characterized by an aggressive clinical course with early metastasis. We report a case of small cell carcinoma of parotid gland in a 82-year-old woman with painless neck mass, who was managed with radiation therapy after surgery and review of relevant literatures.

• Journal of Korean Medical classics
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• v.23 no.4
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• pp.35-37
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• 2010

The Medical Beauty in "Hwangjenaegyeong(黃帝內經)" is characterized by the establishment of the unity of man and nature, human and social unity, the unity of human body organs and meridians, Gi-Blood[氣血] human essence unity, the unity of body and spirit. The harmony of Jang(臟) and Bu(腑), blood and Gi(氣), Eum and Yang and mental and physical health are essential to the beauty, and the harmony of internal and external environment must not be overlooked. In a word, the Medical Beauty in "Hwangjenaegyeong(黃帝內經)" is the thought of strong and handsome which based on cultivating health to prevent disease and aging.