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Effect of storage condition of resin cement on shear bond strength of the orthodontic bracket (레진시멘트의 보관 조건이 치열교정용 브라켓의 전단접착강도에 미치는 영향)

  • Seul-Gi, Yi;Jin-Woo, Kim;Se-Hee, Park;Yoon, Lee;Eung-Hyun, Kim;Kyung-Mo, Cho
    • Journal of Dental Rehabilitation and Applied Science
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    • v.38 no.4
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    • pp.189-195
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    • 2022
  • Purpose: For orthodontic bracket bonding, light curing resin cement is widely used because the process is convenient, and it can be polymerized at the desired time. This study compared the difference of bonding strength of orthodontic resin cement according to storage condition. Materials and Methods: After acid etching the bovine enamel surface with 37% phosphoric acid, 15 orthodontic brackets for mandible incisors were bonded with Ortho Connect and Orthomite LC according to following three conditions; 1) Immediate after 4℃ refrigeration for 3 months (IR), 2) One day room temperature after 4℃ refrigeration for 3 months (OR), 3) Room temperature for 3 months (RT). The shear bond strength was measured with a universal material tester and failure pattern of the specimen was observed. Two-way ANOVA and One-way ANOVA were used at the 95% significance level. Results: Ortho Connect that was applied immediately after refrigeration showed the maximum shear bond strength. Orthomite that was applied immediately after refrigeration showed the lowest shear bond strength, and the group stored at room temperature for three months showed the highest shear bond strength, and the difference between the two groups was significant. Conclusion: Ortho Connect can be used without worrying about bond strength even if it is used immediately after refrigeration, but Orthomite should be kept at room temperature sufficiently after refrigeration.

Adaptable Tropical Japonica High quality New Rice Cultivar 'Japonica 6' (열대지역 적응 고품질 자포니카 벼 신품종 'Japonica 6')

  • Jeong, O-Young;Torollo, Gideon;Bombay, Maurene;Baek, Man-Kee;Ahn, Eok-keun;Hyun, Woong-Jo;Park, Hyun-Su;Jeong, Jong-Min;Cho, Jun-Hyeon;Lee, Jeong-Heui;Yeo, Un-Sang;Lee, Jeom-Sig;Jeong, Eung-Gi;Kim, Choon-Song;Suh, Jung-Pil;Kim, Bo-Kyeong;Lee, Jeom-Ho
    • Journal of the Korean Society of International Agriculture
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    • v.31 no.3
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    • pp.249-254
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    • 2019
  • 'Japonica 6' is a japonica rice variety developed from a cross between 'MS11', the beginning variety adaptable to tropical region, and 'IR86743-28-1-4', an elite line of high yield and good plant type by a Korea(RDA)-IRRI cooperative breeding program at IRRI in 2017. The growth duration of 'Japonica 6' is 121 days from sowing to harvest. It is 10 days later than that of the check variety 'MS11'. The culm length of 'Japonica 6' is 70 cm, and 1,000-brown rice grain weight is 26.7 g. It has a shorter culm and a larger grain. size than that of MS11. 'Japonica 6' is moderately resistant to blast disease but susceptible to bacterial blight, tungro virus and plant hoppers. The milled rice recovery rate of 'Japonica 6' is improved than that of 'MS11'. The head rice rate of 'Japonica 6' is significantly higher than that of 'MS11'. Yield of 'Japonica 6' is averagely 3.59 MT/ha of milled rice in 5 areas of the Philippines. The 'Japonica 6' was registered in Philippines and would be adaptable to the diverse regions of tropical Asia (Registration No in Philippines. BPI-NSIC-2017-Rc 484SR).

'Jungmo1033', a Derivative of High-quality Native Rice Variety 'Jagwangdo' (재래벼 '자광도' 유래 고품질 벼 '중모1033')

  • Jeong, Eung-Gi;Won, Yong-Jae;Ahn, Eok-Keun;Hyun, Ung-Jo;Cho, Young-Chan;Suh, Jung-Pil;Oh, Myoung-Kyu;Lee, Jeom-Ho;Hong, Ha-Cheol;Lee, Chung-Kuen;Jeon, Yong-Hee;Jeung, Ji-Ung;Chung, Hi-Che;Kim, Bo-Kyeong
    • Korean Journal of Breeding Science
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    • v.51 no.1
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    • pp.34-40
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    • 2019
  • 'Jungmo1033', a japonica rice variety, was developed by the rice breeding team at the National Institute of Crop Science (NICS) in 1992. It is derived from a cross between a native variety 'Jagwangdo', which has translucent milled rice and medium maturity; and 'Hwayeong', which is an elite line with bacterial blight resistance and mid-late maturity. The heading date of 'Jungmo1033' was August 10 in the middle plain area of Korea, which was two days later than that of 'Hwaseong'. 'Jungmo1033' has a culm length of 79 cm, which was 5 cm shorter than that of 'Hwaseong', and 105 spikelets per panicle. 'Jungmo1033' showed resistance to bacterial blight (K1, K2, and K3 races) and stripe virus, but susceptibility to the K3a race of bacterial blight, dwarf and black-streaked dwarf viruses, and planthoppers. The milled rice of this variety exhibited translucency and a medium short grain shape. It had an excellent appearance and lower amylose content (19.1%) than that of 'Hwaseong'. The characteristics related to grain milling were better than those of 'Hwaseong', especially head rice milling recovery ratio and head rice ratio (94.8%). 'Jungmo1033' showed a milled rice productivity of 5.38 MT/ha at 11 sites under ordinary cultivation conditions. (Registration No. 5723)

Distribution and Frequency of SSR Motifs in the Chrysanthemum SSR-enriched Library through 454 Pyrosequencing Technology (국화 SSR-enriched library에서 SSR 반복염기의 분포 및 빈도)

  • Moe, Kyaw Thu;Ra, Sang-Bog;Lee, Gi-An;Lee, Myung-Chul;Park, Ha-Seung;Kim, Dong-Chan;Lee, Cheol-Hwi;Choi, Hyun-Gu;Jeon, Nak-Beom;Choi, Byung-Jun;Jung, Ji-Youn;Lee, Kyu-Min;Park, Yong-Jin
    • Journal of the Korean Society of International Agriculture
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    • v.23 no.5
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    • pp.546-551
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    • 2011
  • Chrysanthemums, often called mums or chrysanths, belong to the genus Chrysanthemum, which includes about 30 species of perennial flowering plants in the family Asteraceae. We extracted DNA from Dendranthema grandiflorum ('Smileball') to construct a simple sequence repeat (SSR)-enriched library, using a modified biotin-streptavidin capture method. GS FLX (Genome Sequencer FLX System which provides the flexibility to perform the broad range of applications) sequencing (at the 1/8 run specification) resulted in 18.83 mega base pairs (Mbp) with an average read length of 280.06 bp. Sequence analyses of all SSR-containing clones revealed a predominance of di-nucleotide motifs (16,375, 61.5%) followed by tri-nucleotide motifs (6,616, 24.8%), tetra-nucleotide motifs (1,674, 6.3%), penta-nucleotide motifs (1,283, 4.8%), and hexa-nucleotide motifs (693, 2.6%). Among the di-nucleotide motifs, the AC/CA class was the most frequently identified (93.5% of all di-nucleotide types), followed by the GA/AG class (6.1%), the AT/TA class (0.4%), and the CG/GC class (0.03%). When we analyzed the distribution of different repeat motifs and their respective numbers of repeats, regardless of the motif class, of 100 SSR markers, we found a higher number of di-nucleotide motifs with 70 to 80 repeats; we also found two di-nucleotide motifs with 83 and 89 repeats, respectively, but their product lengths were within optimum size (297 and 300 bp). In future work, we will screen for polymorphisms of possible primer pairs. The results will provide a useful tool for assessing molecular diversity and investigating the population structure among and within Chrysanthemum species.

Feeding Habits of the Largehead Hairtail, Trichiurus japonicus in the Yellow Sea of Korea (우리나라 서해에서 출현하는 갈치(Trichiurus japonicus)의 식성)

  • Seong, Gi Chang;Kim, Do-Gyun;Kang, Da Yeon;Jin, Suyeon;Kim, Hoseung;Soh, Ho Young;Baeck, Gun Wook
    • Korean Journal of Ichthyology
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    • v.34 no.3
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    • pp.179-185
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    • 2022
  • The feeding habits of Largehead hairtail Trichiurus japonicus was studied using 377 specimens collected in the Yellow Sea of Korea. The specimens ranged from 4.5 to 33.7 cm in Anal length (AL). T. japonicus consumed mainly a piscivore, such as Engraulis japonicus [percent index of relative importance (%IRI) =74.1%]. We calculated the trophic level as 3.84 for T. japonicus. Fishes were the main prey items for all seasons. The main fish prey during autumn and winter was E. japonicus, whereas those during spring and summer was Larimichthys polyactis. Fishes were the main prey items for all size groups (<15 cm, 15~20 cm, 20~25 cm, ≥25 cm). T. japonicus also showed size-related dietary shift from Spratelloides gracilis and E. japonicus to L. polyactis and T. japonicus. As the anal length of T. japonicus increased, the mean number of preys per stomach (mN/ST) and the mean weight of preys per stomach (mW/ST) tended to increased (One-way ANOVA, P<0.05). Seasonal and size-related shifts in dietary composition were investigated by PERMANOVA analysis, which showed significant variations among size classes and seasons.

Development of Elite Lines with Improved Eating Quality Using RIL Population Derived from the Korean Weedy Rice, Wandoaengmi6 (국내 잡초벼(완도앵미6) 유래 RILs 집단의 식미 관련 특성분석 및 우량계통 선발)

  • Kim, Suk-Man;Park, Seul-Gi;Park, Hyun-Su;Baek, Man-Kee;Jeong, Jong-Min;Cho, Young-Chan;Suh, Jung-Pil;Lee, Keon-Mi;Lee, Chang-Min;Kim, Choon-Song
    • Journal of the Korean Society of International Agriculture
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    • v.31 no.4
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    • pp.428-436
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    • 2019
  • As the main objective of rice breeding programs, rice eating quality is one of critical factors directly determining the market price and the consumer preference. However, the genetic complexity of eating quality and the difficulty in accurate evaluation often constrain improvement of the eating quality in rice breeding programs. In addition, given that the rice eating quality of current cultivars has already reached some high-level, diversifying of genetic resources are demanded more than ever to improve the rice eating quality. In this study, we developed a recombinant inbred lines (RILs) population derive from Wandoaengmi6, a japonica-type Korean weedy rice with high eating quality and a high degree of glossiness of cooked rice. Year-to-year correlations between the traits in three years were shown normal distribution for major agronomic traits and physicochemical characteristics. After evaluating tested traits related to eating quality procedure, a total of ten lines were ultimately selected from the population. Increasement of the taste of cooked rice (TA) and the overall eating quality (OE) were confirmed in the selected lines, which are caused by alleles derived from Wandoaengmi6 without any linkage drag. These results indicate that the utility of wide genomic resources in Korean landrace could be of application in various rice breeding programs and countermeasure to contribute to properly response to climate change.

Bacterial Blight-Resistant Medium Maturing Rice Cultivar 'Haepum' with High Grain Quality (벼흰잎마름병 저항성 고품질 중생 벼 '해품')

  • Nam, Jeong-Kwon;Park, Hyun-Su;Baek, Man-Kee;Cho, Young-Chan;Kim, Woo-Jae;Kim, Jeong-Ju;Kim, Bo-Kyeong;Kim, Ki-Young;Shin, Woon-Chul;Ko, Jong-Cheol;Lee, Gun-Mi;Park, Seul-Gi;Lee, Chang-Min;Kim, Choon-Song;Suh, Jung-Pil;Lee, Jeom-Ho
    • Korean Journal of Breeding Science
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    • v.51 no.3
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    • pp.222-233
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    • 2019
  • 'Haepum' is a bacterial blight-resistant, medium maturing rice cultivar with high grain quality. It was derived from a cross between 'Iksan493' (cultivar name 'Jinbaek') and the F1 cross between 'Iksan495' ('Dacheong') and 'Iksan496' ('Jungmo1005'). Of these three cultivars, 'Jinbaek' is a bacterial blight-resistant mid-late maturing rice cultivar with high grain quality, 'Dacheong' is a mid-late maturing rice cultivar with multiple resistance to disease and insects, and 'Jungmo1005' is a mid-late maturing rice cultivar with lodging tolerance. To develop fixed lines, the anther culture method was applied to F1 plants. The cultivar 'Haepum' was selected using the pedigree method, yield trials, and local adaptability tests. The heading date of 'Haepum' was August 11th, three days earlier than that of 'Nampyeong' cultivar. Haepum' is a cultivar tolerant to lodging and it has short culms. Due to its low rate of viviparous germination, 'Haepum' could be useful for preventing pre-harvest sprouting in cultivation of medium maturing rice in the southern plain area of Korea. 'Haepum' carries two bacterial blight resistance genes (Xa3 and xa5), and in our study, it exhibited high-level and broad-spectrum resistance against bacterial blight, including K3a, the most virulent race in Korea. 'Haepum' is also resistant to the rice stripe virus and moderately resistant to rice blast. The yield of 'Haepum' was similar to that of 'Nampyeong'. 'Haepum' showed excellent grain appearance and good taste of cooked rice, and therefore it could contribute to the development of bacterial blight-resistant rice cultivars of improved quality. 'Haepum' would be suitable for cultivation in the southern plain area of Korea as well as in bacterial blight-prone areas. (Registration No. 6068)

A Wide Region of Tropical Asia Adaptable Japonica Rice 'Asemi' (아시아 광지역 적응성 자포니카 벼 '아세미')

  • Jeong, Eung-Gi;kang, Kyeong-Ho;Hong, Ha-Cheol;Cho, Young-Chan;Jung, O-Young;Jeon, Yong-Hee;Chang, Jae-Ki;Lee, Jeom-Ho;Won, Yong-Jae;Yang, Un-Ho;Jung, Kuk-Hyun;Yeo, Un-Sang;Kim, Bo-Kyeong
    • Journal of the Korean Society of International Agriculture
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    • v.31 no.1
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    • pp.76-81
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    • 2019
  • 'Asemi' is a rice variety derived from a cross between 'Jinmibyeo' which has translucent milled rice and medium maturity and 'Cheolwon46', an elite line with high yield and early maturity by the rice breeding team at NICS, RDA in 2013. The heading date of 'Asemi' is August 1, six days earlier than the check variety 'Hwaseong'. It has 82 cm culm length and 109 spikelets per panicle. 'Asemi' is resistant to blast disease, stripe virus and tungro virus, but susceptible to other viruses and planthoppers. The milled rice of this variety exhibits translucent, clear non-glutinous endosperm and short grain shape. It has protein content (6.7%) higher than 'Hwaseong', and amylose content (19.5%) similar to 'Hwaseong'. The milled rice recovery rate of 'Asemi' is similar to that of 'Hwaseong'. However, the head rice rate of 'Asemi' is higher than that of 'Hwaseong'. Milled rice yield of 'Asemi' is 5.23 MT/ha in ordinary cultivation. ' Asemi' could be adaptable to the wide region of tropical Asia (Registration No. 5639).

Development of Near-Isogenic Line of japonica Rice Cultivar Saenuri without Lipoxygenase-3 (새누리 벼 품종 배경 lipoxygenase-3 결핍 자포니카 근동질계통 개발)

  • Park, Hyun-Su;Lee, Keon-Mi;Kim, Ki-Young;Kim, Jeong-Ju;Shin, Woon-Cheol;Baek, Man-Kee;Kim, Choon-Song;Park, Seul-Gi;Lee, Chang-Min;Suh, Jung-Pil;Cho, Young-Chan
    • Korean Journal of Breeding Science
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    • v.51 no.3
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    • pp.190-200
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    • 2019
  • It is reported that the absence of lipoxygenase-3 (LOX-3) may contribute to a reduction in stale flavor after the storage of rice. To improve the quality of stored rice of the Korean japonica rice cultivar, we conducted a breeding program to develop near-isogenic rice without LOX-3 in the genetic background of Saenuri, a mega variety of Korea. In the first step of the breeding program, we used a donor parent of LOX-3 null, Daw Dam, and a recurrent japonica parent, Sindongjin, to develop HR27873-AC12 by backcross (BC1), color test for introgression of lox-3, and anther culture for rapid fixation. In the second step, we used the donor parent, HR27873-AC12, and the recurrent parent, Saenuri, to develop HR28896-31-3-1-1 by backcross (BC1), marker-assisted selection (MAS) for lox-3, and phenotypic selection (PS) for agronomic traits. Finally, in the third step, we developed HR30960-186-2-1-2-1 (Jeonju624), derived from a cross between Saenuri and HR28896-31-3-1-1, by MAS for lox-3 and PS with high selection pressure for agronomic characteristics. Jeonju624 was confirmed with the introgression of lox-3 by molecular marker. Jeonju624 was a mid-late maturing rice with similar agronomic characteristics to Saenuri, lodging tolerance with short culm, erect plant architecture, and resistance to bacterial blight and rice stripe virus. The yield components of Jeonju624 were mostly similar to Saenuri, except for the 1,000-grain weight of brown rice. The appearance of the grain of Jeonju624 was better than that of Saenuri, and the characteristics of cooked rice were similar to those of Saenuri. In the genetic background analysis using 406 KASP (Kompetitive Allele-Specific PCR) markers, Jeonju624 was confirmed to be the near-isogenic line (NIL) of Saenuri with a 95.8% recovery rate. Jeonju624 is the NIL of Saenuri without LOX-3, and overcomes the linkage drag of Daw Dam with similar agronomic characteristics and genetic background to Saenuri. Jeonju624 can be utilized as a practical cultivar to improve the quality of stored rice, breeding material for the introgression of lox-3, and genetic material to elucidate the effect of introgressed genes.

High Grain Quality Mid-late Maturing Rice Cultivar 'Yechan' with Lodging Tolerance and Multiple Disease Resistance (내도복 복합내병 최고품질 중만생 벼 '예찬')

  • Baek, Man-Kee;Park, Hyun-Su;Nam, Jeong-Kwon;Cho, Young-Chan;Kim, Ki-Young;Kim, Jeong-Ju;Kim, Woo-Jae;Shin, Woon-Chul;Jeung, Ji-Ung;Kim, Choon-Song;Jeong, Jong-Min;Lee, Keon-Mi;Park, Seul-Gi;Lee, Chang-Min;Suh, Jung-Pil;Lee, Jeom-Ho
    • Korean Journal of Breeding Science
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    • v.51 no.4
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    • pp.504-514
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    • 2019
  • 'Yechan' is a high grain quality mid-late maturing rice cultivar with lodging tolerance and multiple disease resistance. It was a derived from a cross between 'Hopum' and 'Iksan537' (cultivar name 'Haepum'). 'Hopum' is a high grain quality mid-late maturing rice cultivar with strong lodging tolerance and 'Haepum' is a high grain quality medium maturing rice cultivar with multiple disease resistance. To shorten the breeding period, another culture method was applied to the F1 plants. 'Yechan' was selected through the pedigree method, yield trials, and local adaptability tests, with a high selection pressure for grain quality, lodging, and disease resistance. The heading date of 'Yechan' was August 14, one day later than that of 'Nampyeong'. 'Yechan' is a cultivar tolerant to lodging and it has short culms. It has multiple disease resistance against rice blast, rice stripe virus, and bacterial blight, including the K3a race, the most virulent race in Korea. The yield of 'Yechan' was similar to that of 'Nampyeong'. 'Yechan' showed excellent grain appearance, superior taste when cooked, and enhanced milling performance; thus, we concluded that it could contribute to the improvement of Korean japonica rice cultivar quality. 'Yechan', a high grain quality mid-late maturing rice cultivar with lodging tolerance and multiple disease resistance, would be suitable for cultivation in the southern plain area in Korea and has been utilized in the breeding programs aimed at enhancing the grain quality and stability for the cultivation of Korean japonica rice (Registration No. 7647).