• Journal of Microbiology and Biotechnology
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• 제8권6호
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• pp.601-605
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• 1998

For the resolution of L-carnitine from DL-carnitine, resting cells of Enterobacter sp. NH-104, which had a higher capacity of D-carnitine decomposition, were harvested at maximal specific activity of D-carnitine decomposition of 47.05 unit/mg cell. The cells were frozen at $-80^{\circ}C$ to assess functions as enzyme sources. Optimal concentration of cells and DL-carnitine were 17 g/$\ell \; and \; 20 g/\ell$, respectively, and reaction buffer was best at 75 mM of Tris. HCl. Optimal temperature and pH were $36^{\circ}C$ and 8.2, respectively. When the reaction at optimal conditions was carried out for 14 h, the optical purity was 98.21 %, and the quantity and yield of remaining L-carnitine were 4.432 g/$\ell$ and 44.32%, respectively.

• 한국잠사곤충학회지
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• 제49권2호
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• pp.37-42
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• 2007

락토페린 cDNA 유전자를 도입시킨 누에 형질전환 실험을 수행한 결과, 다음과 같은 결과를 얻을 수 있었다. 1. 사람 GI-101 세포주의 mRNA로부터 클로닝 된 락토페린 cDNA 유전자의 개시코돈 ATG와 종결코돈 TAA를 포함하는 open reading frame(2,136 bp) 영역을 확인하였다. 2. Sf9 배양세포의 조추출물 시료에 의한 Western blot 분석 결과, 락토페린으로 추정되는 약 80kDa의 단백질 발현을 확인하였다. 3. 누에 형질전환에 높은 전이효율과 활성을 나타내는 트랜스포존을 이용한 전이벡터 pPIGA3GFP를 개조하여 락토페린 cDNA를 삽입시킨 전이벡터 pPT-HLf를 구축하였다. 4. DNA 미량 주사법에 의한 누에 형질전환 개체의 발현 비율은 약 6.7% 정도를 나타냈다. 5. 형질전환 누에(G0) 동일한 세대간 교배 및 처리하지 않은 성충간의 역교배에 의한 차세대(G1) 개체로부터 락토페린 유전자와 동일한 크기의 2.1 kb DNA 단편을 확인 할 수 있었으며, 형질전환 G1 세대의 조추출물 시료에 의한 Western blot 분석 결과, 표준 락토페린 항체와 반응하는 약 80 kDa의 단백질 발현을 확인할 수 있었다.

• 한국식품영양과학회지
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• 제36권8호
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• pp.1094-1097
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• 2007

주파수 20 Hz와 0.18 g의 가속도에 해당하는 진동을 1L 유리병에 담겨진 600 g의 물김치에 가하였다. $-3^{\circ}C$와 $-6^{\circ}C$에서 동결의 진행정도를 관찰하였고, $10^{\circ}C$에서는 총산과 $CO_{2}$ 발생을 측정하여 발효진행 정도를 측정하였다. 진동이 없는 대조구를 같은 온도조건에서 두어서 진동의 효과를 비교하였다. $-3^{\circ}C$와 $-6^{\circ}C$에서의 진동은 동결의 과정을 지연시키고, 계속되는 저장에서 작은 얼음결정의 혼합체를 유지하는 데에 있어서 긍정적인 결과를 보였다. $10^{\circ}C$에서 진동은 산의 생성과 $CO_{2}$ 발생을 촉진하여 숙성시간을 단축시키고 시원한 맛을 주는 데 긍정적인 역할을 할 수 있는 것으로 평가되었다.

• 한국균학회지
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• 제6권2호
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• pp.11-14
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• 1978

Attempts were made to investigate the sterol components of Boletus aerus Bull. which grows wildly in Korea. Its carpophores were collected in Gyeong-Gi Province and extracted with chloroform and methanol. A sterol was isolated and identified as ergosterol by T.L.C., G.L.C. and chemical tests.

• Biomolecules & Therapeutics
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• 제22권5호
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• pp.400-405
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• 2014

G-protein coupled receptor 119 (GPR119) has emerged as a novel target for the treatment of type 2 diabetes mellitus. GPR119 is involved in glucose-stimulated insulin secretion (GSIS) from the pancreatic b-cells and intestinal cells. In this study, we identified a novel small-molecule GPR119 agonist, HD047703, which raises intracellular cAMP concentrations in pancreatic ${\beta}$-cells and can be expected to potentiate glucose-stimulated insulin secretion from human GPR119 receptor stably expressing cells (CHO cells). We evaluated the acute efficacy of HD047703 by the oral glucose tolerance test (OGTT) in normal C57BL/6J mice. Then, chronic administrations of HD047703 were performed to determine its efficacy in various diabetic rodent models. Single administration of HD047703 caused improved glycemic control during OGTT in a dose-dependent manner in normal mice, and the plasma GLP-1 level was also increased. With respect to chronic efficacy, we observed a decline in blood glucose levels in db/db, ob/ob and DIO mice. These results suggest that HD047703 may be a potentially promising anti-diabetic agent.

• 한국식품과학회지
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• 제36권2호
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• pp.226-232
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• 2004

음료 제조를 위한 수박 원료 희석 농도, 당, 구연산, 비타민 C 첨가농도, 살균 방법, 천연 색소 추출 방법, 풍미증진 재료의 첨가량에 따른 색과 맛에 대하여 조사하였다. 주스 제조를 위한 조건을 실험한 결과 수박의 맛과 색이 남아있는 수박즙의 최적 희석 농도는 40%이었고, 음료로서 적정 당도는 $12^{\circ}Brix$였으며, 산미와 항산화 효과를 위한 구연산과 비타민 C첨가 농도는 0.5+0.3 g/L 또는 1.0+0.3 g/L이 적절하였다. 가열살균과 시간을 비교한 결과 수박 음료를 $70^{\circ}C$이상의 온도에서 살균 시 수박 고유의 적색도가 감소되었으며, 관능 검사 결과 $60^{\circ}C$ 10-30분 또는 $70^{\circ}C$에서 10 분간 살균 시 양호하였다 또 $60^{\circ}C$이상으로 살균 시 $30^{\circ}C$에서 한 달 동안 저장 후 처리시간에 관계없이 부패된 것은 발견되지 않았다. 천연 색소 추출 방법에 있어서는 열수 추출에 비해 알콜 추출은 전반적으로 효과가 낮았으며, 첨가 재료에 있어서도 알콜 추출보다는 열수 추출에 유리한 재료인 복분자와 오미자가 효과적이었고 복분자 20 g/L와 오미자 30g/L를 열수추출한 것을 7:3으로 배합하여 음료를 제조했을 때 가장 기호도가 높았다. 추후 다른 재료를 탐색 할 때도 수용성 가용분이 많은 재료가 적합하다고 생각된다. 본 실험에서는 주스 제조 시 침전의 문제를 해결하기 위해 희석과 예열 처리로 응고되는 침전물을 미리 여과하는 방법을 사용했으나 이 보다 더 좋은 개선 방법을 찾기 위한 연구가 앞으로 더 필요하리라 생각된다.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2006년도 추계학술대회 논문집 전기물성,응용부문
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• pp.165-166
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• 2006

343 kV GIS 다빈도 조작 CB 내부에 접점이 마모되어 많은 파티클이 발생한다. 이러한 파티클이 GIS 네부에 존재할 때 어떠한 영향을 미치는지 이론적으로 근거를 제시하고, 이를 실험적으로 검증을 하였다. 현장에서 수거한 파티클을 설치 운영중인 PDMS 장비와 HAEFELY 장비를 이용하여 실험용 GIS에 삽입후 발생하는 현상들을 동시에 비교 분석하고자 한다.

• The Korean Journal of Physiology and Pharmacology
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• 제14권1호
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• pp.29-35
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• 2010

We have shown that myosin light chain kinase (MLCK) was required for the off-contraction in response to the electrical field stimulation (EFS) of feline esophageal smooth muscle. In this study, we investigated whether protein kinase C (PKC) may require the on-contraction in response to EFS using feline esophageal smooth muscle. The contractions were recorded using an isometric force transducer. On-contraction occurred in the presence of $N^G$-nitro-L-arginine methyl ester (L-NAME), suggesting that nitric oxide acts as an inhibitory mediator in smooth muscle. The excitatory composition of both contractions was cholinergic dependent which was blocked by tetrodotoxin or atropine. The on-contraction was abolished in $Ca^{2+}$-free buffer but reappeared in normal $Ca^{2+}$-containing buffer indicating that the contraction was $Ca^{2+}$ dependent. 4-aminopyridine (4-AP), voltage-dependent $K^+$ channel blocker, significantly enhanced on-contraction. Aluminum fluoride (a G-protein activator) increased on-contraction. Pertussis toxin (a $G_i$ inactivator) and C3 exoenzyme (a rhoA inactivator) significantly decreased on-contraction suggesting that Gi or rhoA protein may be related with $Ca^{2+}$ and $K^+$ channel. ML-9, a MLCK inhibitor, significantly inhibited on-contraction, and chelerythrine (PKC inhibitor) affected on the contraction. These results suggest that endogenous cholinergic contractions activated directly by low-frequency EFS may be mediated by $Ca^{2+}$, and G proteins, such as Gi and rhoA, which resulted in the activation of MLCK, and PKC to produce the contraction in feline distal esophageal smooth muscle.

• 방사선산업학회지
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• 제12권4호
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• pp.355-363
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• 2018

A novel $N_3S_1$ chelate, Pro-Lys-Cys (PKC) to cyclic RGD to radiolabel with $^{99m}Tc$ was conjugated in an effort to decrease the high intestinal accumulation observed for $^{99m}Tc$-labeled PGC-RGD. The target specificity of the resulting PKC-RGD was similar to that of PGC-RGD as determined by a cell binding assay and a competition binding assay. The $^{99m}Tc$ radiolabeling of PKC-RGD resulted in radiochemical yields of 98% under mild conditions at high specific activities. Biodistribution data in normal mice clearly showed a significant decrease in intestinal uptake at 2 h postinjection for the $^{99m}Tc-PKC-c$ (RGDyK) compared to the $^{99m}Tc-GC-c$ (RGDyK) (from $19.65%ID{\cdot}g^{-1}$ to $7.31%ID{\cdot}g^{-1}$ for the GI tract). The $^{99m}Tc-PKC-c$ (RGDyK) biodistribution was also shown by a higher retention of radioactivity in the whole body, but with kidney accumulation over 8-fold higher than observed with $^{99m}Tc-PGC-c$ (RGDyK) at 2 h ($12.62%ID{\cdot}g^{-1}$ for PKC-RGD and $1.54%ID{\cdot}g^{-1}$ for PGC-RGD, respectively). These results show that the biodistribution may be altered especially concerning lipophilicity resulting in renal rather than hepatobiliary excretion. This comparative study made it possible to explore the effects of lipophilicity on the biodistribution of $^{99m}Tc$-labeled c (RGDyK) through the use of different tripeptide $N_3S_1$ chelators. Therefore, $^{99m}Tc-PKC-c$ (RGDyK) may be an attractive alternative for the in vivo imaging of integrin receptors.

• Journal of Acupuncture Research
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• 제19권2호
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• pp.78-91
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• 2002

Objective : This study was designed to investigate the anti-cancer effects of bee venom on melanoma in C57BL mice. Materials and Methods : For the induction of melanoma, C57BL mice were treated by DMBA(7, 12-dimethylbenz[a]anthracene). Each group of C57BL mouse was treated with DMBA $50{\mu}g$, $75{\mu}g$, $100{\mu}g$ respectively once a week for 15 weeks. Tumor generation in each group of 10 mice was observed. Cumulative curves were showed in the density and frequency of skin tumor generation. To know the effects of pre-treatment of bee venom on tumor generation by DMBA treatment(frequency of tumor generation), Each group of C57BL mouse was pretreated and treated with bee venom $5{\mu}{\ell}$, $25{\mu}{\ell}$, $50{\mu}{\ell}$ respectively once a week for 3 weeks, whereafter each mouse was treated with DMBA $100{\mu}g$ once a week for 15 weeks. Results and Conclusion (1) There was chemotherapeutic effect, but not chemopreventive effect. (2) Cpp32 activity was increased by $50{\mu}{\ell}$ bee venom treatment. (3) Bee venom treatment inhibited expression of cell-cycle regulating, growth-promoting genes such as c-Jun, c-Fos, and Cyclin Dl, and increased tumor suppressors p53 and p21/Wafl. (4) Bee venom treatment activated expression of a representative apoptosis-inducing gene Bax.