• 제목/요약/키워드: GFS

검색결과 79건 처리시간 0.022초

$CCl_4$로 급성 간손상을 유도한 백서에서 인삼엽과 경추출물의 간기능 개선과 항산화 작용 (Improvement of Liver Function and Suppressed Lipid Peroxidation of Extract from Ginseng Folium and Stem in Acute $CCl_4$ Intoxicated Rats)

  • 이민경;박성혜;서의석;김기영
    • 동의생리병리학회지
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    • 제18권5호
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    • pp.1485-1489
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    • 2004
  • Panax ginseng is the one of best famous phytochemical plant in the world and it's various positive effects such as antioxidant, regulation of immunity are very well known. In this study, we investigated primary the cell viability and morphological change and secondary an antioxidative effect and liver function improvement of extract from Ginseng folium and stem in CCl4 intoxicated rats. The NCTC cell line were used for cell viability and sirius red staining before the animal experiment. The female Sprague-Dawley rats (90-100g) were divided into 3 groups (Normal, AC: CCl₄ treated group, GFS: CCl₄+ extract of Ginseng folium and stem treated group) and acute liver damage was developed by one time administration of CCl₄ mixture (0.5㎖/rat). The liver tissue and sera were collected and used for quantitative measurement of enzyme activity (AST, ALT, ALP, BUN), MDA and Hyp. As a result, cell viability in GFS treated group (in concentration of 3.33-33.33㎎ GFS/200㎕ medium) was 180.9-241.0% significantly and dose dependently higher than in control group. And potential state of cell growth and differentiation and no criteria of cytoplasm lysis and nucleus breaking were observed in control and GFS group. The parameters of liver function (AST and ALP) in sera of GFS group showed significantly 93% and 67.6% lower than AC group (p<0.005-0.05). And the level of ALT and BUN showed fast similar in AC group and GFS group. The concentration of MDA in liver was decreased 576.5% significantly in GFS group when compared with AC group (p<0.005). The content of Hyp in GFS group is merely lower than in AC group. In conclusion, the water extract of Ginseng folium and stem such as Ginseng radix may be possessed the antioxidative effect and improvement of liver function in CCl₄ intoxicated rats.

생쥐 후기상실배의 Vitrification에 관한 연구 (Cryopreservation of Mouse Late Morulae by Vitrification)

  • 강민수
    • 한국수정란이식학회지
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    • 제8권1호
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    • pp.1-7
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    • 1993
  • This study was carried out to investigate the survival rates of late mouse molulae frozen in the state of vitrification and then thawed after equilibrating them separately in EFS 40, GFS 40 and DFS 40 at 1$0^{\circ}C$. The results obtained are as follows : 1. Freezing in the state of vitrification and thawing late mouse molulae after equilibrating them at l0$0^{\circ}C$ in EFS 40 for 30 seconds, one minute and two minutes, we obtained survival rates of 76.7%, 96.7% and 100%, respectively. 2. Freezing and thawing them after equilibrating at 1$0^{\circ}C$ in GFS 40 for 30 seconds, one minute and two minutes, we obtained survival rates of 60%, 96.7% and 10%, respectively. These results are as similar as in the case of EFS 40. 3. Freezing and thawing them after equilibrating at l$0^{\circ}C$ in DFS 40 for 30 seconds and one minute, we obtained survival rates of 62.1% and 0%, respectively. These results represent lower survival rates than those obtained with EFS 40 and GFS 40. In conclusion, even equilibrating late mouse molulae in EFS 40 and GFS 40 at 1$0^{\circ}C$ for more than one minute gives a survival rate of more than 97%, while equilibrating them in DFS 40 at 1$0^{\circ}C$ for more than one minute results in a 0% survival rate, which means that DFS 40 has a strong toxicity.

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Planning of Gap Filler Networks in Satellite DMB Systems for Wireless Multimedia Services

  • Noh, Sun-Kuk;Yun, Tae-Soon;Choi, Dong-You
    • Journal of Communications and Networks
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    • 제13권5호
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    • pp.494-498
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    • 2011
  • Satellite digital multimedia broadcasting (S-DMB) systems use gap fillers (GFs) to provide wireless multimedia services to non-line-of-sight locations. GFs act as repeaters, and S-DMB systems require GF networks in order to guarantee mobile reception. Each GF covers a cell or sector. In order to provide contiguous coverage of an area comprising two or more cells or sectors, multiple GFs are needed. However, when multiple GFs are situated close to each other, interference is likely to occur. As a result, in this study, we have investigated system-level environments for planning the design of interference-free GF networks in S-DMB systems. Our investigations revealed that S-DMB services are unavailable because of quality deterioration caused by interference when the delay attributable to a GF and the satellite signals exceeds ${\pm}$256 chips and the distance between the GF and its reception terminal is greater than 4.6 km. On the basis of this analysis, we conducted a field test that confirmed that the above-mentioned time delay can be controlled in such a way as to ensure high quality S-DMB services.

The Improvement of Laying Productivity and Egg Quality according to Providing Germinated and Fermented Soybean for a Feed Additive

  • Shin, Jin-Ho;Park, Jung-Min;Kim, Jin-Man;Roh, Kwang-Soo;Jung, Woo-Suk
    • 한국축산식품학회지
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    • 제32권4호
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    • pp.404-408
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    • 2012
  • This study was performed to investigate the effects of laying productivity and egg quality according to providing germinated and fermented soybean (GFS) as feed additive. Among the strain, we selected Monascus purpureus KCCM 12002 so that inoculated in soybean and fermented for 48 h at $20^{\circ}C$. A total of two-hundred forty 70-wk-old Hy-Line Brown layers were divided into four groups (4 treatment${\times}$6 replication${\times}$10 birds each) and fed diets containing 0 (as control) (T1), 0.5% (T2), 1.0% (T3) or 2.0% GFS (T4) for 6 wk. The laying productivity, egg quality and blood property in the egg yolk were experimented. There were no significant differences in the laying productivity, relative liver and spleen weights, egg yolk color and eggshell strength among another groups. The eggshell color, eggshell thickness and haugh unit significantly increased in the GFS-supplemented group (p<0.05) compared to control. However, no significant differences were observed in the blood property after supplementation. The amount of lactic acid bacteria present during storage increased by providing of GFS (p<0.05) compare to control group. Our study results suggested that GFS can be used as a favorable feed additive and feedstuff for the productivity of high quality eggs and promoted relative industry.

Feeding pattern, biochemical, anthropometric and histological effects of prolonged ad libitum access to sucrose, honey and glucose-fructose solutions in Wistar rats

  • Virgen-Carrillo, Carmen Alejandrina;Moreno, Alma Gabriela Martinez;Rodriguez-Gudino, Juan Jose;Pineda-Lozano, Jessica Elizabeth
    • Nutrition Research and Practice
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    • 제15권2호
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    • pp.187-202
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    • 2021
  • BACKGROUND/OBJECTIVES: The exposure to sucrose in rats has mimic abnormalities attributed to metabolic syndrome (MetS). The effects of honey bee and "free" glucose and fructose, have not been explored in this context. The aim was to expose Wistar rodents to sucrose solution (SS), honey solution (HS) and fructose/glucose solution (GFS) at 30% to assess their effects. SUBJECTS/METHODS: HS (n = 10), SS (n = 10) and GFS (n = 10) groups were formed. Solutions were ad libitum along 14-weeks. RESULTS: Between solutions consumptions, honey was significantly 42% higher (P = 0.000), while similar consumption was observed among GFS and SS. The feeding pattern of HS consumption was irregular along experiment; while the food intake pattern showed the similar trend among groups along time. Non statistical differences were obtained in any biochemical and anthropometric measure, however, a higher concentration of leptin (721 ± 507 pg/mL), lower concentration of total cholesterol (TC; 48.87 ± 2.41 mg/100 mL), very low density lipoprotein (VLDL; 16.47 ± 6.55 mg/100 mL) and triglycerides (82.37 ± 32.77 mg/100 mL) was obtained in SS group. For anthropometric values, HS showed less total adipose tissue (AT; average 26 vs. 31-33 g) and adiposity index (average 6.11 vs. 7.6). Due to sugar-sweetened beverages consumption increases the risk for the development of chronic diseases; correlations between fluid intake and anthropometric and biochemical parameters were assessed. A moderate correlation was obtained in groups with the weight of total AT and solution intake; for the weight gain in GFS group and for triglycerides in HS and GFS. The highest hepatic tissue damage was observed in SS group with multiple intracytoplasmic vacuoles, atypia changes, moderate pleomorphism and hepatocellular necrosis. CONCLUSIONS: In spite of the significantly higher consumption of HS, biochemical, anthropometrical and histological effects were not remarkably different in comparision to other sweeteners.

주행속도 추정을 위한 Genetic Fuzzy System의 개발 (The Development of Genetic Fuzzy System for Estimating Link Traveling Speed)

  • 윤여훈;이홍철;김용식
    • 대한산업공학회지
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    • 제29권1호
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    • pp.32-40
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    • 2003
  • In this study, we develop the Genetic Fuzzy System(GFS) to estimate the link traveling speed. Based on the genetic algorithm, we can get the fuzzy rules and membership functions that reflect more accurate correlation between traffic data and speed. From the fact that there exist missing links that lack traffic data, we added a Case Base Reasoning(CBR) to GFS to support estimating the speed of missing links. The case base stores the fuzzy rules and membership functions as its instances. As cases are accumulated, the case base comes to offer appropriate cases to missing links. Experiments show that the proposed GFS provides the more accurate estimation of link traveling speed than existing methods.

사료작물 재배지에서 초지식생대가 PO4-P 및 토양유실 저감에 미치는 영향 (Effect of Grass Filter Strips on PO4-P and Soil Loss in Runoff from Forage Cropland)

  • 조남철;윤세형;김기용;이기원;김맹중;육완방;정민웅
    • 한국초지조사료학회지
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    • 제30권4호
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    • pp.309-316
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    • 2010
  • 본 연구는 사료작물 재배지에 가축분뇨를 시용한 사료작물 재배지에서 초지식생대를 설치에 따른 $PO_4$-P와 토양 유실 저감 효과를 구명하기 위하여 수행되었다. 본 시험은 국립축산과학원 초지사료과 시험포장(천안)에서 경사가 약 10%에서 ${\pm}3%$인 자연 경사지를 이용하여 2007년부터 2009년까지 3년간 수행하였다. 가축분뇨 시용은 화학비료, 우분퇴비 및 돈분퇴비를 시용하였으며, 초지식생대의 길이는 5 m, 10 m 및 15 m의 길이로 설치하였다. 초지식생대 길이에 따른 유거수 중의 $PO_4$-P의 농도는 초지식생대의 길이가 길어질수록 줄어드는 경향을 보였다. 유거수 중의 평균 $PO_4$-P 농도는 초지식생대 0 m인 지점에 비해 초지식생대의 길이가 10 m 및 15 m 일 경우 $PO_4$-P의 농도가 유의적으로 감소하였으나(p<0.05), 초지식생대의 길이 10 m와 15 m 사이의 유실량의 유의적인 감소는 일어나지 않았다. 가축분뇨 시용에 따른 2008~2009년 평균 $PO_4$-P 농도는 화학비료 > 우분퇴비 > 돈분퇴비 순으로 높은 경향을 보였으나, 통계적인 유의성은 나타나지 않았다. 초지식생대 설치에 따른 2년 평균 토양 유실량은 길이가 5 m, 10 m, 15 m로 길어질수록 감소하였으며, 5 m 및 10 m에 비해 15m 지점에서 유의적인 토양 유실량 감소를 가져왔다(p < 0.05). 따라서 경사지에서 초지식생대의 설치는 가축분뇨를 시용한 사료작물 재배 시 강우로 유실되는 $PO_4$-P와 토양의 유실을 줄일 수 있을 것으로 사료된다.

마우스 상실배의 Vitrification에 관한 연구 (Vitrification of Mouse Morulae)

  • 강민수;손시환
    • 한국가축번식학회지
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    • 제15권3호
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    • pp.173-177
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    • 1991
  • In vitro survival of the mouse morulae frozen by vitrification method(Kasai et al., 1990) was investigated in the present study. The embryos were plunged into LN2 directly after exposure to the vitrification solutions(EFS, GFS and DFS). The results were obtained as follows. The viability of morulae after freezing and thawing was high in EFS(96.7∼100.0%) and GFS vitrification solution(93.3∼96.7%), and the lowest in DFS vitrification solution(0.00∼0.03%).

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아라미드 스트랩으로 프리스트레싱 횡구속된 RC 원형기둥의 일축압축거동에 관한 실험적 연구 (An Experimental Study on Uniaxial Compressive Behavior of RC Circular Columns Laterally Confined with Prestressing Aramid Fiber Strap)

  • 한상훈;홍기남;이재범
    • 콘크리트학회논문집
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    • 제21권2호
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    • pp.159-168
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    • 2009
  • 본 연구에서는 기존의 연구방법을 개선한 프리스트레스가 도입된 아라미드 스트랩으로 횡 구속된 RC 원형기둥의 일축압축 거동에 대하여 강도와 변위에 의한 강성 및 구속효과에 대하여 연구하였으며, 그 프리스트레싱 보강방법 및 과정에 대하여 비교적 상세히 소개하였다. 이 방법으로 보강된 기둥의 파괴형태는 초기 능동 구속압의 작용으로 인하여 변위 증가율이 매우 낮으며 이후 최종 파괴시까지도 그 형상이 유지되는 매우 안정적인 파괴형태를 보이게 된다. 파괴하중 비교 결과 일반 RC 기둥과 비교하여 73% 이상 강도증진 효과를 나타내었다. 또한 변형 증가율이 매우 작으며 이로 인한 변위구속효과가 매우 우수함을 확인할 수 있었다. 기존의 보강방법 (CFS, GFS)과의 비교를 통하여, 상대적으로 작은 면적으로 우수한 강도증진을 보이며 강성증가로 인한 구속효과 역시 우수하며 체적 증가율도 팽창하지 않는 결과를 보였다. 특히, 콘크리트 팽창 이후부터 발휘되는 기존 방법의 구속효과와는 달리, 초기부터 도입되는 프리스트레스트 긴장력에 의한 초기변형 구속효과가 매우 탁월하며, 최종 파괴시까지 체적 변형률이 팽창하지 않는 안정적인 결과를 나타낸다.

Inhibition of Cell Growth and Mitochondrial Activity in Human Gingival Fibroblasts by LED-Generated Red Light Exposure

  • Kim, Hee-Jeong;Hwang, Jung-Min;Kwak, So-Yeong;Kim, Jong-Ghee;Jeon, Young-Mi;Lee, Jeong-Chae
    • International Journal of Oral Biology
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    • 제34권4호
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    • pp.185-190
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    • 2009
  • This study examined the effects of red light generated from a light emitting diode (LED) upon proliferation and mitochondrial stress in human gingival fibroblasts (hGFs). Cells were exposed to LED-generated red light at a clinically relevant intensity and distance with a 610-630 nm wavelength for various times (0-48 min). At different exposure times, cells were processed for the analysis of succinate dehydrogenase (SDH) activity, proliferation, mitochondrial membrane potential (MMP) and cytotoxicity. Cell cycle progression was also investigated by flow cytometry after staining with propidium iodide. Red light exposure was found to inhibit SDH activity and DNA synthesis in hGFs in a time-dependent manner. Light exposure also reduced the MMP levels in these cells and this was closely associated with a $G_0/G_1$ arrest. In contrast, exposure of hGFs to red light for 48 min led to a dramatic loss of MMP with an attendant increase in cytotoxicity. These findings demonstrate that LED-generated red light may cause mitochondrial stress and growth inhibition in hGFs during tooth whitening therapy, depending on the length of the exposure.