• BMB Reports
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• v.46 no.12
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• pp.588-593
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• 2013

Apoptosis, programmed cell death, is a process involved in the development and maintenance of cell homeostasis in multicellular organisms. It is typically accompanied by the activation of a class of cysteine proteases called caspases. Apoptotic caspases are classified into the initiator caspases and the executioner caspases, according to the stage of their action in apoptotic processes. Although caspase-3, a typical executioner caspase, has been studied for its mechanism and substrates, little is known of caspase-6, one of the executioner caspases. To understand the biological functions of caspase-6, we performed proteomics analyses, to seek for novel caspase-6 substrates, using recombinant caspase-6 and HepG2 extract. Consequently, 34 different candidate proteins were identified, through 2-dimensional electrophoresis/MALDI-TOF analyses. Of these identified proteins, 8 proteins were validated with in vitro and in vivo cleavage assay. Herein, we report that HAUSP, Kinesin5B, GEP100, SDCCAG3 and PARD3 are novel substrates for caspase-6 during apoptosis.

• Geomechanics and Engineering
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• v.33 no.1
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• pp.1-9
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• 2023

The unconfined compression strength (UCS) of soils is commonly used either before or during the construction of geo-structures. In the pre-design stage, UCS as a mechanical property is obtained through a laboratory test that requires cumbersome procedures and high costs from in-situ sampling and sample preparation. As an alternative way, the empirical model established from limited testing cases is used to economically estimate the UCS. However, many parameters affecting the 1D soil compression response hinder employing the traditional statistical analysis. In this study, gene expression programming (GEP) is adopted to develop a prediction model of UCS with common affecting soil properties. A total of 79 undisturbed soil samples are collected, of which 54 samples are utilized for the generation of a predictive model and 25 samples are used to validate the proposed model. Experimental studies are conducted to measure the unconfined compression strength and basic soil index properties. A performance assessment of the prediction model is carried out using statistical checks including the correlation coefficient (R), the root mean square error (RMSE), the mean absolute error (MAE), the relatively squared error (RSE), and external criteria checks. The prediction model has achieved excellent accuracy with values of R, RMSE, MAE, and RSE of 0.98, 10.01, 7.94, and 0.03, respectively for the training data and 0.92, 19.82, 14.56, and 0.15, respectively for the testing data. From the sensitivity analysis and parametric study, the liquid limit and fine content are found to be the most sensitive parameters whereas the sand content is the least critical parameter.

• Computers and Concrete
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• v.31 no.3
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• pp.253-265
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• 2023

In this research, the gene expression programming (GEP) technique was employed to provide a new model for predicting the maximum loading capacity of concrete-encased steel (CES) columns. This model was developed based on 96 CES column specimens available in the literature. The six main parameters used in the model were the compressive strength of concrete (f_c), yield stress of structural steel (f_ys), yield stress of steel rebar (f_yr), and cross-sectional areas of concrete, structural steel, and steel rebar (A_c, A_s and A_r respectively). The performance of the prediction model for the ultimate load-carrying capacity was investigated using different statistical indicators such as root mean square error (RMSE), correlation coefficient (R), mean absolute error (MAE), and relative square error (RSE), the corresponding values of which for the proposed model were 620.28, 0.99, 411.8, and 0.01, respectively. Here, the predictions of the model and those of available codes including ACI ITG, AS 3600, CSA-A23, EN 1994, JGJ 138, and NZS 3101 were compared for further model assessment. The obtained results showed that the proposed model had the highest correlation with the experimental data and the lowest error. In addition, to see if the developed model matched engineering realities and corresponded to the previously developed models, a parametric study and sensitivity analysis were carried out. The sensitivity analysis results indicated that the concrete cross-sectional area (A_c) has the greatest effect on the model, while parameter (f_yr) has a negligible effect.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.10
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• pp.5775-5781
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• 2013

Background: The gastroenteropancreatic neuroendocrine neoplasm (GEP-NEN) is the most common type of neuroendocrine neoplasm. We summarized data in our centre to investigate the clinicopathological features, diagnostic methods, therapeutic approaches and prognosis for this neoplasm to increase knowledge of this disease in Asian populations. Method: A total of 122 patients treated at Sun Yet-san Memorial Hospital of Sun Yat-sen University between January 2000 and December 2011 were analyzed retrospectively. Results: Pancreas was the most common site of involvement (65/122, 53.3%); this disease has no special symptoms; positive rates of chromogranin A (CgA) and synaptophysin (Syn) were 81.1% and 87.7%, respectively. The positive rate of Syn had statistical difference among the three grades, but not CgA. Some 68 patients had G1 tumors, 32 G2 tumors and 22 G3 tumors, and Chi-square test showed that higher grading was correlated with worse prognosis (${\chi}^2=32.825$, P=0.0001). A total of 32 patients presented with distant metastasis, and 8 cases emerged during following up. Cox proportional hazards regression modeling showed that the tumor grade (P=0.01), lymphatic metastasis (P=0.025) and distant metastasis (P=0.031) were predictors of unfavorable prognosis. The overall 5-year survival rate was 39.6%, the 5-year survival rate of G1 was 55.7%, and the G2 and G3 were 34.2% and 0%, respectively. Conclusions: The incidence of gastroenteropancreatic neuroendocrine tumors has risen over the last 12 years. All grades of these diseases metastasize readily, and further research regarding the treatment of patients after radical surgery is needed to prolong disease-free survival.

• Applied Biological Chemistry
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• v.44 no.4
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• pp.211-214
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• 2001

Utilizing the foreign gene expression system of Drosophila melanogaster Schneider line 2(S2) cell, the degree of transient protein and mRNA expression was examined with different terminators. In the case of transient expression, the expression level of green fluorescent protein(GFP) was the highest when the transfection agent was eliminated and then cultivated for 36 to 48 hr. The terminators(SV40 p(A), SV40 small T-antigen and human gastrin 3'UTR) of the expression vector system were each cloned with endostatin; thereafter, the expression levels of the endostatin and its mRNA were compared. When the expression levels of endostatin were compared 36 hr after transfection, the SV40 p(A) terminator showed the highest expression level of endostatin and its mRNA.

• Journal of the Korean Geographical Society
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• v.28 no.2
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• pp.163-178
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• 1993

The purpose of this paper is to provide a better understanding of the unprecedented reform movement of geographic education in the United States since 1980 and extract some implications from this movement for geographic education in Korea. For the purpose, the history to this movement was reviewed through following three stages. In the first stage(1980~1984: form :HSGP" to :"Guideline"), the voluntary improvement movement appeared at California and the orgni-zational movement began in 1982 such as the Committee on Geography and International Knowledge. The national educational refrom imperatives, presented at "A Nation at Risk", and "Back to Basics" movement provided good opportunities to resurrect geography as a basic subject. For next real resurrection movement, the very important document "Guidelines for Geographic Education" was published at 1984. In the second stage(1985~1989: from "Guide-lines" to "Public"), the "Guideline" gave power-full motives and foci for reconstructiong the contents of geography, especially by the five fundamental themes(Location, Place, Relation-ships within Places, Movement, and Region). Also GENIP as the symbol of unity of all four major geography organization(AAG, NCGE, NGS, AGS) contributed to expanding and stren-gthening geography education. Also Geography Educagtion Program of NGS was a smart and well organized program to improve geographic education through it's a five strategies: Grass-roots organization(Alliances), Teacher education, Pu-blic awareness, Educational materials develo-pment, Targeted outreach to education decision-makers. In the late 1980s, the last focus of movement was the Public awareness and Edua-ction decision-making. In the third stage(1990-present: from "Public" to "Core Subject"), the initiative pendulum swung from geography organization to nation curricu-lum. In this National Curriculum, Geography was approved as a "Core Subject" and The 1994 National Geography Assessment Framework was constructed to assess the outcome of student's education in geography in grades, 4,8, and 12. Some Implications extracted from the process and contents of renaissance movement of geogr-aphic education in the Uinted States since 1980 are as follows. First, It shows the importance of the unity and target assignment among the geography organization. Second, interactive relationship between the academic geography and school geography develops each other. Third, teacher education, including pre-service education, including pre-service education and in-service education, is a key element to improve the quality of geography. And teacher organization is a good clearing house to exchange information for good geography. Forth, the positive and active response to changes in socketies such as globalism and inter-nationalizing, national education policy, and the trend of pedagogy is needed to rejuvenate geo-graphic education. Above all, we need to establish a well organized and powerfull program, sophisticated activities strategies, and long-term implementa-tion plan if we want more and better school geography.