• Biomolecules & Therapeutics
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• 제27권2호
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• pp.193-200
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• 2019

Ceramide metabolism is known to be an essential etiology for various diseases, such as atopic dermatitis and Gaucher disease. Glucosylceramide synthase (GCS) is a key enzyme for the synthesis of glucosylceramide (GlcCer), which is a main ceramide metabolism pathway in mammalian cells. In this article, we developed a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to determine GCS activity using synthetic non-natural sphingolipid C8-ceramide as a substrate. The reaction products, C8-GlcCer for GCS, could be separated on a C18 column by reverse-phase high-performance liquid chromatography (HPLC). Quantification was conducted using the multiple reaction monitoring (MRM) mode to monitor the precursor-to-product ion transitions of m/z $588.6{\rightarrow}264.4$ for C8-GlcCer at positive ionization mode. The calibration curve was established over the range of 0.625-160 ng/mL, and the correlation coefficient was larger than 0.999. This method was successfully applied to detect GCS in the human hepatocellular carcinoma cell line (HepG2 cells) and mouse peripheral blood mononuclear cells. We also evaluated the inhibition degree of a known GCS inhibitor 1-phenyl-2-decanoylamino-3-morpholino-1-propanol (PDMP) on GCS enzymatic activity and proved that this method could be successfully applied to GCS inhibitor screening of preventive and therapeutic drugs for ceramide metabolism diseases, such as atopic dermatitis and Gaucher disease.

• BMB Reports
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• 제43권8호
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• pp.524-529
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• 2010

Glucocorticoids (GCs) are useful drugs for the treatment of various diseases, but their use for prolonged periods can cause severe side effects such as osteoporosis. GCs have a direct effect on bone cells, where they can arrest bone formation, in part through the inhibition of osteoblast. On the other hand, GCs potently suppress osteoclast resorptive activity by disrupting its cytoskeleton based on the inhibition of RhoA, Rac and Vav3 in response to macrophage colony-stimulating factor. GCs also interfere with microtubule distribution and stability, which are critical for cytoskeletal organization in osteoclasts. Thus, GCs inhibit microtubule-dependent cytoskeletal organization in osteoclasts, which, in the context of bone remodeling, further dampens bone formation.

• 천문학논총
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• 제30권2호
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• pp.265-266
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• 2015

A search for luminous white dwarfs (WDs) in several nearby Galactic globular clusters (GCs) was carried out using the deep and homogeneous photometric catalog of Galactic GCs taken with the ACS/WFC aboard the Hubble Space Telescope (HST) by Sarajedini et al. and Anderson et al- It resulted in the identification of luminous WD candidates in the GCs M13 (NGC 6205) and M22 (NGC 6656). The purpose of the present study is to identify luminous WDs in the deep and homogeneous V versus V - I color-magnitude diagrams (CMDs) of several nearby Galactic GCs taken with the ACS/WFC aboard the HST. Using photometric data for the GCs M13 and M22 that are now in the public domain, the V versus V - I CMDs of the GCs M13 and M22 were constructed. Many spurious detections in the CMDs were removed using the photometric quality parameters qfit(V) and qfit(I), and a radial restriction was applied to the CMDs to remove the central stars with higher photometric errors due to central crowding. From each resultant V versus V - I CMD of the GCs M13 and M22, a dozen or so luminous WD candidates were identified. They were confirmed as stellar objects in the accompanying ACS/WFC images and their positions in the CMDs were in the bright part of the DA WD cooling curve. Therefore, the luminous WD candidates in the GCs M13 and M22 seem to be true luminous WDs, and spectroscopic observations are needed to confirm their true identity.

• 한국정보통신학회논문지
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• 제25권10호
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• pp.1353-1358
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• 2021

IoT(Internet of Things) 기술은 통신, 마이크로 프로세서 그리고 임베디드 소프트웨어 기술의 발전과 융합으로 인해 탄생 되었다. 그에 따른 응용 중 하나로 UAV(Unmanned Aerial Vehicle, 무인 항공기) 시스템에도 적용되고 있다. 운용상 유인용 항공기(manned aircraft)는 지상에 있는 관제 시스템을 통해 운항의 지시를 받아, 조종사가 직접 비행 기체를 제어하는 방식으로 운영되지만, UAV는 GCS(Ground Control System, 지상 제어시스템)를 통해 직접 비행 제어를 받는다. 따라서 UAV와 GCS 간의 통신을 위한 프로토콜로 개방형인 MAVLink (Macro Air Vehicle Link)가 주로 사용이 되고 있으나, 범용이고 공개적이다 보니 일부구간에서 제삼자에 의해 쉽게 노출되어 비행체의 상태 및 기타 제어 정보 등의 정보 보안에 취약하다. 본 제안은 UAV를 안전하게 운영하기 위한 GCS 내 통신메시지의 암호화 적용 방안을 제시한다.

• 천문학회보
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• 제40권2호
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• pp.33.2-33.2
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• 2015

The color distribution functions (CDFs) of globular clusters (GCs) in individual early-type galaxies show great diversity in their morphology. Based on the conventional "linear" relationship between colors and metallicities of GCs, the inferred GC metallicity distribution functions and thus their formation histories should be as diverse as they appear. In contrast, an alternative scenario rooted in the "nonlinear" nature of the color-to-metallicity transformation finds the various CDFs pointing systematically to a simple picture, i.e., such a high degree of variety stems predominately from only one parameter, the mean metallicity of GCs. The simulated CDFs of GCs aimed to reproduce 67 massive early-type galaxies from the ACS Virgo & Fornax Cluster Survey show that over 70% of the CDFs concur fully with the nonlinearity scenario. We discuss our new findings in terms of early-type galaxy formation in the cluster environment.

• Animal cells and systems
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• 제6권3호
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• pp.247-252
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• 2002

The effect of paraquat (PQ, 1,1＇-dimethyl-4,4＇-bipyridium) on the histogene-sis and glycoconjugates (GCs) properties of the pyloric region of the stomach in a perinatal rat was examined by histological and histochemical methods. Oral administration of PQ (9 mg/kg per day in 0.2 mL of D.W.) on 7 to 14 days of gestation revealed growth retardation with significant reductions in the length of pyloric gland and their pit. As for histochemical properties of GCs in the pyloric region of the stomach, the PQ-treated rats showed some differences, such as delayed initial appearance of the sulfated GCs and lectin affinities compared with the vehicle group. These different GCs properties in the surface and gastric pit were usually detected in the fetal rats and more prominent and evident differences were revealed in the gland epithelium of the early postnatal rat. These results suggest that maternal PQ administration causes intrauterine growth retardation asso-ciated with delayed histogenesis and GCs immaturation of pyloric mucosa in developing rat.

• 천문학회보
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• 제40권2호
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• pp.57.3-57.3
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• 2015

The formation of the Milky Way stellar halo is thought to be the result of merging and accretion of building blocks such as dwarf galaxies and massive globular clusters. Recently, Deason et al. (2015) suggested that the Milky Way outer halo formed mostly from big building blocks, such as dwarf spheroidal galaxies, based on the similar number ratio of blue straggler (BS) stars to blue horizontal-branch (BHB) stars. Here we demonstrate, however, that this result is seriously biased by not taking into detailed consideration on the formation mechanism of BHB stars from helium enhanced second-generation population. In particular, the high BS-to-BHB ratio observed in the outer halo fields is most likely due to a small number of BHB stars provided by GCs rather than to a large number of BS stars. This is supported by our dynamical evolution model of GCs which shows preferential removal of first generation stars in GCs. Moreover, there are sufficient number of outer halo GCs which show very high BS-to-BHB ratio. Therefore, the BS-to-BHB number ratio is not a good indicator to use in arguing that more massive dwarf galaxies are the main building blocks of the Milky Way outer halo. Several lines of evidence still suggest that GCs can contribute a signicant fraction of the outer halo stars.

• Journal of Trauma and Injury
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• 제21권2호
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• pp.71-77
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• 2008

Purpose: The Glasgow Coma Scale (GCS), though it is widely used for triage, has been criticized as being unnecessarily complex. Recently, a 3-point Simplified Motor Score (SMS, defined as obeys commands=2; localizes pain=1; withdrawals to pain or worse=0) was developed from the motor component of the GCS and was found to have a similar test performance for triage after traumatic brain injury when compared with the GCS as the criterion standard. The purpose of this study was to validate the SMS. Methods: We analyzed the patients who visited Kyungpook National University Hospital emergency center after traumatic brain injury from 2006 January to 2006 June. The test performance of the GCS, its motor component, and SMS relative to three clinically relevant traumatic brain injury outcomes (abnormal brain CT scans, Abbreviated Injury Scale $(AIS){\geq}4$, and mortality) were evaluated with areas under the receiver operating characteristic curves (AUCs). Results: Of 504 patients included in the analysis, 25.6% had an abnormal brain CT scans, 13.1% had $AIS{\geq}4$, and 5.0% died. The AUCs for the GCS, its motor component, and SMS with respect to the abnormal CT scans were 0.776, 0.715, and 0.716, and respectively, those for $AIS{\geq}4$ and mortality, were 0.969, 0.973, and 0.968, and 0.931, 0.909, and 0.909, respectively. Conclusion: The 3-point SMS demonstrated similar test performance when compared with the 15-point GCS score and its motor component for triage after traumatic brain injury in our populations.

• Asian-Australasian Journal of Animal Sciences
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• 제24권9호
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• pp.1204-1210
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• 2011

Objective of this study was to examine the effect of sodium nitroprusside (SNP), a nitric oxide (NO) donor on steroid synthesis, growth and apoptosis of buffalo granulosa cells (GCs) in vitro. Follicular fluid of antral follicles (3-5 mm diameter) was aspirated and GCs were cultured in 0 (control), $10^{-3}$, $10^{-5}$, $10^{-7}$, $10^{-9}\;M$ of SNP for 48 h. To evaluate whether this effect was reversible, GCs were cultured in presence of $10^{-5}\;M$ SNP+1.0 mM $N^{\omega}$-nitro-L-arginine methyl ester (L-NAME) a NO synthase (NOS) inhibitor or hemoglobin (Hb, $1.0{\mu}g$) as NO scavenger. Nitrate/nitrite concentration was evaluated by Griess method, progesterone and estradiol concentrations by RIA and apoptosis by TUNEL assay. SNP ($10^{-3}$, $10^{-5}$, $10^{-7}\;M$) significantly (p<0.05) inhibited estradiol and progesterone synthesis, growth, disorganized GCs aggregates and induced apoptosis in a dose dependent manner. However, $10^{-9}\;M$ SNP induced the progesterone synthesis and stimulated GCs to develop into a uniform monolayer. Combination of SNP $10^{-5}$ M+L-NAME strengthened the inhibitory effect while, SNP+Hb together reversed these inhibitory effects. In conclusion, SNP at greater concentrations ($10^{-3}$, $10^{-5}$ and $10^{-7}\;M$) has a cytotoxic effect and it may lead to cell death whereas, at a lower concentration ($10^{-9}\;M$) induced progesterone synthesis and growth of GCs. These findings have important implications that NOS derived NO are involved at physiological level during growth and development of buffalo GCs which regulates the steroidogenesis, growth and apoptosis.

• 한국산학기술학회:학술대회논문집
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• 한국산학기술학회 2012년도 춘계학술논문집 1부
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• pp.22-24
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• 2012

The tools that classify the severity of patients based on the prediction of mortality include APACHE, SAPS, and MPM. Theses tools rely crucially on the evaluation of patients' general clinical status on the first date of their admission to ICU. Nursing activities are one of the most crucial factors influencing on the quality of treatment that patients receive and one of the contributing factors for their prognosis and safety. The purpose of this study was to identify the goodness-of-fit of CPSCS of critical patient severity classification system(CPSCS) and Glasgow coma scale(GCS) and the clinical usefulness of its death rate prediction. Data were collected from the medical records of 187 neurological patients who were admitted to the ICU of C University Hospital. The data were analyzed through $x^2$ test, t-test, Mann-Whitney, Kruskal-Wallis, goodness-of-fit test, and ROC curve. In accordance with patients' general and clinical characteristics, patient mortality turned out to be statistically different depending on ICU stay, endotracheal intubation, central venous catheter, and severity by CPSCS. Homer-Lemeshow goodness-of-fit tests were CPSCS and GCS and the results of the discrimination test using the ROC curve were $CPSCS_0$, .734, $GCS_0$,.583, $CPSCS_{24}$,.734, $GCS_{24}$, .612, $CPSCS_{48}$,.591, $GCS_{48}$,.646, $CPSCS_{72}$,.622, and $GCS_{72}$,.623. Logistic regression analysis showed that each point on the CPSCS score signifies1.034 higher likelihood of dying. Applied to neurologically ill patients, early CPSCS scores can be regarded as a useful tool.