• 한국동물위생학회지
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• 제16권2호
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• pp.103-110
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• 1993

The Aeromonas strains were isolated from diseased cultured catfsh (Silurus asotus) in the ponds near by Gochang of Chunbuk. The present isolates were identified as A. hydrophila based on their biological and biochemica1 characteristics. The isolates of A. hydrophila were named GC-1, GC-2, GC-3, GC-4 and GC-5, Five strains were grew optimally at temperatures $35^{\circ}C,$ pH 7.5 and in 0.5 to l% NaCl. This bacterium(GC-1) was injected into health catfish in order to prove the causative agent of ascites and haemorrhagic ulcers. The symptoms in cat fish infected by this challenge method were observed to be very similar to the symptoms of a natural infection, but controls did not show any abnormal symptoms during the experimental period. At 24 post-injection, the red spot developed around the injection site and the haemorrhagic ulcers was extended near the gill and ventral fin. Five strains were tested for drug sensitivity by plate method. All strains were sensitivity to gentamicin and resistant to penicillin, streptomycin and tetracycline.

• 분석과학
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• 제23권3호
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• pp.278-283
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• 2010

본 연구에서는 국제적으로 관심의 대상이 되고 있는 폴리염화비페닐류 함유 액상폐기물의 효율적인 관리를 위해 국내에서 사용되고 있는 L2000DX을 이용한 스크리닝, 신속 GC/ECD 및 기존 GC/ECD 분석방법을 절연유 15건에 적용하였다. 분석 결과 L2000DX을 이용한 경우에는 4.4~182.8 mg/L의 농도 범위로 Aroclor 1242 환산농도가 검출된 반면, 기존 GC/ECD 및 신속 GC/ECD를 이용한 분석 결과 각각 불검출~14.63 mg/L 및 불검출~13.03 mg/L로 나타났다. GC/ECD 분석방법들 간에는 약 4 mg/L이하에서 거의 차이가 없었으나, 국내의 규제농도인 2 mg/L부근의 검출농도에서는 일부 시료의 경우 기존 GC/ECD 분석결과(2.71 mg/L)에 비해 신속 GC/ECD 방법이 다소 낮은(2.49 mg/L) 측정치를 보여 국내 규제기준 2 mg/L부근에서 저평가되는 경향이 있는 것으로 나타났다.

• 분석과학
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• 제26권1호
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• pp.80-85
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• 2013

본 연구는 유해화학물질관리법에 지정되어 있는 사고대비물질에 대한 실험실적 분석방법을 정립하기 위한 것으로, GC/MS 분석이 가능한 25종 사고대비물질을 대상으로 정량분석을 수행하였다. GC/MS 정량분석결과 69종 사고대비물질 중 산화프로필렌(propylene oxide) 등 21개 물질에 대한 결정계수($r^2$)는 0.998 이상을 보였고, 포스겐(phosgene)의 결정계수는 0.994, 노말-부틸아민(n-butyl amine) 외 1종은 0.987, 에틸렌디아민(ethylene diamine)은 0.958의 결정계수를 보였다. 동일한 GC/MS 분석 조건으로 표준물질을 분석한 결과 총 25종에 대한 검량선이 작성되었다. 현재 사고대비물질에 대한 분석 연구가 필요한 상황에서, 본 연구결과는 화학사고 사후 환경 영향 조사를 위한 정량분석에 도움이 될 것으로 판단된다.

• 분석과학
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• 제25권6호
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• pp.364-369
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• 2012

Isotianil은 쌀 도열병을 방제하기 위한 새로이 개발된 식물활성 살균제로 다른 식물활성제에 비해 소량으로도 장시간의 효과를 나타내는 특징을 가지고 있다. 신규 농약 Isotianil에 대한 기준은 국내의 경우 2010년 쌀에 대하여 0.1 mg/kg으로 최초 설정되었으며, 이에 따른 식품 중 안전관리를 위해 Isotianil을 분석하기위한 검사법을 개발하였다. 검체 일정량에 acetonitrile을 가하고 균질화하여 대상농약을 추출하였으며, 액-액분배하고 florisil 카트리지를 이용한 고체상 추출 후 GC-NPD 및 GC-MSD로 분석하였다. 분석결과 농산물에 의한 간섭물질은 없었으며, 대표 농산물 현미 등 6 종에 대한 Isotianil의 분석 시험법의 정량한계와 직선성은 각각 0.05 mg/kg과 0.999($r^2$)이었다. 회수율은 대표 농산물에서 70.0~103.9%이었으며, 분석오차는 10% 미만으로 CODEX 잔류분석 기준에 적합하였다.

• 동아시아식생활학회지
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• 제4권1호
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• pp.79-86
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• 1994

Food quality in food processing and storage were affected by the kinds of phenolics involved. To analyze phenolics of Chebulae Fructus by the way of GC-MSm methylation and trimethylsilyation(TMS) are necessary. The methods of methylation were dimethyl sulfate method and diazomethane method. so this study was undertaken to research the better methylation method before measuring GC-MS. But dimethyl sulfate method of methylation was not sufficient to analyze phenolics. So the phenolics of Chebulae Fructus were analyzed by the diazomethane methylation method and TMS with the pyridine, N-O-bis-trimethylsilyl-acetamide(BSTFA) and trimethylchlorosilane(TMCS). With the exception of pyrogallol and phloroglucinol in insoluble phenolics of Chebulae Fructus, the greater part of phenolics. analysis could be analyzed by GC-MS in company with diazomethane methylation method and TMS.

• 한국연초학회지
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• 제36권1호
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• pp.12-19
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• 2014

A matrix solid-phase dispersion (MSPD) method was developed for extracting and cleaning-up the selected agrochemicals in tobacco using gas chromatography-mass spectrometry with selected ion monitoring (GC/MS-SIM). Different parameters of the method were investigated and optimized, such as the type of solid-phase (alumina, $C_{18}$ and Florisil) and eluent (acetone, acetonitrile, ethylacetate and n-hexane). The best results were obtained using 0.5 g of tobacco sample, 1.0 g of $C_{18}$ as dispersant sorbent, 1.0 g of Florisil as clean-up sorbent and acetonitile saturated with n-hexane as eluting solvent. The method was validated using tobacco samples fortified with agrochemicals at their different concentration levels. This method gave good linearity for the selected agrochemicals of ranging from $0.01{\mu}g/mL$ to $0.1{\mu}g/mL$. Recoveries of the selected agrochemicals in tobacco were more than 80 % and reproducibilities were found to be better than 10 % RSD. Those results suggested that the analytical procedure including MSPD method in combining with GC/MS could be applicable to the rapid determination often the selected agrochemicals in tobacco.

• 분석과학
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• 제32권3호
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• pp.88-95
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• 2019

This study was conducted to establish the analytical method for the determination of cyanide in blood, urine, lung and skin tissues in rats. In order to detect or quantify the sodium cyanide in above biological matrixes, it was derivatized to Pentafluorobenzyl cyanide (PFB-CN) using pentafluorobenzyl bromide (PFB-Br) and then reaction substance was analyzed using gas chromatography mass spectrometer (GC/MS)-SIM (selected ion monitoring) mode. The analytical method for cyanide determination was validated with respect to parameters such as selectivity, system suitability, linearity, accuracy and precision. No interference peak was observed for the determination of cyanide in blank samples, zero samples and lower limit of quantification (LLOQ) samples. The lowest limit detection (LOD) for cyanide was $10{\mu}M$. The linear dynamic range was from 10 to $200{\mu}M$ for cyanide with correlation coefficients higher than 0.99. For quality control samples at four different concentrations including LLOQ that were analyzed in quintuplicate, on six separate occasions, the accuracy and precision range from -14.1 % to 14.5% and 2.7 % to 18.3 %, respectively. The GC/MS-based method of analysis established in this study could be applied to the toxicokinetic study of cyanide on biological matrix substrates such as blood, urine, lung and skin tissues.

• 한국식품과학회지
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• 제21권6호
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• pp.749-754
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• 1989

탱자(Poncirus trifoliata)의 향기성분을 분석하기 위해 gas co-distillation 방법으로 정유를 추출한 후 GC/retention index(RI)와 GC/MS를 이용해 multi-dimensional analysis를 행하였다. Gas co-distillation 방법으로 탱자의 향도 그대로 지니며 기기분석에 적합한 정유를 얻을 수 있었다. 향기성분의 확인작업에는 GC/RI와 GC/MS를 상보적으로 사용한 multi-dimensional analysis가 효과적이었으며 GC/RI로만 확인할 경우 극성이 다른 두 가지 column을 사용해서 신뢰도를 높일 수 있었다. 탱자에 다량 함유되어 있는 향기성분들은 limonene, myrcene, ${\beta}-caryophllene,\;trans-{\beta}-ocimene,\;{\beta}-pinene$, 3-thujene, 7-geranyloxycoumarin 등으로 밝혀졌으며 확인된 30개의 성분 중 cis-3-hexenyl acetate, n-hexyl acetate, 2-methyl acetophenone, elixene 그리고 elemicine 등은 이제까지 감귤류에서는 발견되지 않았던 새로운 성분들이다.

• 한국군사과학기술학회지
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• 제17권6호
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• pp.828-835
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• 2014

GC-TSQ CI technique was applied for analysis of samples for the $32^{nd}$ OPCW proficiency test. Eight chemical weapon convention(CWC) related chemicals were identified by product ion mode analysis with GC-TSQ in the samples. Choice of specific precursor ion made it possible to supply selective total ion chromatograms(TICs) of target molecule. GC-TSQ CI anaylsis technique was useful method for chemical warfare agent verification because analysis selectivity was improved by choice of mother molecule as precursor ion and gave mass spectra.

• Food Quality and Culture
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• 제1권1호
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• pp.13-17
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• 2007

The volatile flavor compounds of Saussurea lappa C.B. Clarke, a perennial, aromatic and medicinal herbaceous plant of the Asteraceae family, were isolated by the hydro distillation extraction method using a Clevenger-type apparatus, and analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC/MS). The plant yielded a light yellow colored oil (0.02%, v/w). From S. lappa C.B. Clarke root oil, sixty-three volatile flavor compounds were tentatively identified, among which sesquiterpene was predominant (21.70%). The identified compounds of the root oil constituted 87.47% of the total peak area. From the constituents making up more than 5% of the volatile flavor components, a long-chain aldehyde, (7Z, 10Z, 13Z)-7, 10, 13-hexadecatrienal, was the most abundant volatile flavor compound (21.20%), followed by dehydrocostuslactone (10.30%) belonging to sesquiterpene lactone, valerenol (5.30%) and vulgarol B (5.06%).