• Title/Summary/Keyword: GC analysis

Search Result 2,188, Processing Time 0.036 seconds

Attractiveness of Plautia stali (Hemiptera: Pentatomidae) Aggregation Pheromone Produced in Korea (국산 갈색날개노린재 집합페로몬의 유인력)

  • Jang, Sin-Ae;Cho, Jin-Hoon;Park, Chung-Gyoo
    • The Korean Journal of Pesticide Science
    • /
    • v.14 no.2
    • /
    • pp.164-169
    • /
    • 2010
  • Methyl (E,E,Z)-2,4,6-decatrienoate is the aggregation pheromone of brown-winged stink bug Plautia stali (Hemiptera: Pentatomidae). Attractiveness of the pheromone synthesized in Korea was compared with that of synthesized in Japan in the field. A lure filled with hexane was used as a control treatment. Catches in trap baited with Korean pheromone was significantly higher than that baited with Japanese one only in one orchard out of three sweet persimmon orchards in 2008. However there was no difference in trap catches between Korean and Japanese pheromones at three sites in 2009. Gas chromatography analysis showed that the components of both pheromones were not different each other. Monitoring of P. stali seasonal fluctuation using Korean pheromone showed that the bugs were most captured in August, 2008 and in September, 2009.

A New Single Nucleotide Polymorphism in the IGF-I Gene and Its Association with Growth Traits in the Nanjiang Huang Goat

  • Zhang, Chunxiang;Zhang, Wei;Luo, Hailing;Yue, Wenbin;Gao, Mingyu;Jia, Zhihai
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.21 no.8
    • /
    • pp.1073-1079
    • /
    • 2008
  • The objectives of this study were to identify polymorphisms of insulin-like growth factor I (IGF-I) gene and to investigate their association with growth traits in Nanjiang Huang goats. Five hundred and ninety-two animals were used to detect the polymorphisms in the complete coding sequence, part of introns and the 5'-regulatory region of the IGF-I gene by means of PCR-SSCP. A new single nucleotide polymorphism (G to C transversion) was identified at intron 4 of the IGF-I gene in the goats. Two alleles and three genotypes were observed in this group. The frequency of G and C alleles was 54.6 and 45.4%, respectively. The statistical analysis showed that polymorphism of the IGF-I gene had a significant association (p<0.05) with birth weight (BW), body weight at 6 months (W6) and at 12 months (W12), heart girth at 2 months (G2), body length at 6 months (L6), wither height at 6 months (H6) and at 12 months (H12) and heart girth at 12 months (G12). The goats with genotype CC had significantly higher BW, W6, W12, G2, L6, H6, H12 and G12 than those with genotype GC and had significantly higher W12, H6, H12 and G12 than those with genotype GG. Therefore, genotype CC may be the most advantageous for growth traits in the Nanjiang Huang goat. However, no significant association between SNP genotypes and other growth traits was observed. These results indicated that the SNP marker of the IGF-I gene may be a potential molecular marker for growth traits in Nanjiang Huang goats.

Transfer of Orally Administered Terpenes in Goat Milk and Cheese

  • Poulopoulou, I.;Zoidis, E.;Massouras, T.;Hadjigeorgiou, Ioannis
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.25 no.10
    • /
    • pp.1411-1418
    • /
    • 2012
  • The objective of the present study was to investigate the relationships between terpenes… intake and their presence in animal tissues (blood and milk) as well as in the final product (cheese). Eight dairy goats were divided in two balanced groups, representing control (C) and treatment (T) group. In T group oral administration of a mixture of terpenes (${\alpha}$-pinene, limonene and ${\beta}$-caryophyllene) was applied over a period of 18 d. Cheese was produced, from C and T groups separately, on three time points, twice during the period of terpenes… oral administration and once after the end of experiment. Terpenes were identified in blood by extraction using petroleum ether and in milk and cheese by the use of solid phase micro-extraction (SPME) method, followed by GC-MS analysis. Chemical properties of the milk and the produced cheeses were analyzed and found not differing between the two groups. Limonene and ${\alpha}$-pinene were found in all blood and milk samples of the T group after a lag-phase of 3 d, while ${\beta}$-caryophyllene was determined only in few milk samples. Moreover, none of the terpenes were traced in blood and milk of C animals. In cheese, terpenes' concentrations presented a more complicated pattern implying that terpenes may not be reliable feed tracers. We concluded that monoterpenes can be regarded as potential feed tracers for authentification of goat milk, but further research is required on factors affecting their transfer.

Component Analysis of Softwood Vinegar (침엽수 목초액의 성분분석)

  • ;;;Sano Yoshihiro
    • Journal of Korea Foresty Energy
    • /
    • v.20 no.1
    • /
    • pp.28-34
    • /
    • 2001
  • To analyze chemical compositions of softwood vinegar prepared with continuous carbonized kiln, the chemical compositions were analyzed by GC-MS spectrometry. The results were summarized as follow : 1. The amounts of methylalcohol and acetic acid and of vinegar were 0.12% and 0.8% respectively, and acidity was 0.85 2. Perfume components of vinegar were frufual, 5-meayl-2-furancarboxyaldehyde, 2,3-pentanedione, 2-butanol, 2,3-dihydrofuran, 1-(2-furanyl)-etanone, benzaldehyde, 2-furan carboxyaldehyde and acetic acid. 3 Vinegar prepared from softwood, so that murk amount of guaiacyl compound and phenol derivetives are produced from lignin and extractives was analγzed. 4 The yield of 4-methyl-di-tert-butylphenol was the highest in the nutural and carbonyl and acetic acid in the acid fractions, 3-ethylpentane in the basic fraction, and guaiacol in the phenolic fraction.

  • PDF

Acacia ferruginea Inhibits Tumor Progression by Regulating Inflammatory Mediators-(TNF-α, iNOS, COX-2, IL-1β, IL-6, IFN-γ, IL-2, GM-CSF) and Pro-Angiogenic Growth Factor-VEGF

  • Sakthivel, Kunnathur Murugesan;Guruvayoorappan, Chandrasekaran
    • Asian Pacific Journal of Cancer Prevention
    • /
    • v.14 no.6
    • /
    • pp.3909-3919
    • /
    • 2013
  • The aim of the present investigation was to evaluate the effect of A ferruginea extract on Dalton's lymphoma ascites (DLA) induced tumours in BALB/c mice. Experimental animals received A ferruginea extract (10 mg/kg.b.wt) intraperitoneally for 14 consecutive days after DLA tumor challenge. Treatment with extract significantly increased the life span, total white blood cell (WBC) count and haemoglobin (Hb) content and decreased the level of serum aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), gamma glutamyl transferase (${\gamma}$-GT) and nitric oxide (NO) in DLA bearing ascites tumor models. In addition, administration of extract significantly decreased the tumour volume and body weight in a DLA bearing solid tumor model. The levels of pro-inflammatory cytokines such as tumor necrosis factor-alpha (TNF-${\alpha}$), interleukin-1 beta (IL-$1{\beta}$), interleukin-6 (IL-6) and granulocyte monocyte-colony stimulating factor (GM-CSF), as well as pro-angiogenic growth factors such as vascular endothelial growth factor (VEGF) and inducible nitric oxide synthase (iNOS) were elevated in solid tumour controls, but significantly reduced by A ferruginea administration. On the other hand, the extract stimulated the production of interleukin-2 (IL-2) and interferon-gamma (IFN-${\gamma}$) in animals with DLA induced solid tumours. Increase in $CD4^+$ T-cell population suggested strong immunostimulant activity for this extract. GC/MS and LC/MS analysis showed quinone, quinoline, imidazolidine, pyrrolidine, cyclopentenone, thiazole, pyrazole, catechin and coumarin derivatives as major compounds present in the A ferruginea methanolic extract. Thus, the outcome of the present study suggests that A ferruginea extract has immunomodulatory and tumor inhibitory activities and has the potential to be developed as a natural anticancer agent.

A Study on Oxidative Degradation of Chlorophenols by Heat Activated Persulfate (열적활성화된 과황산에 의한 염화페놀의 산화분해특성 연구)

  • Son, JiMin;Kwon, Hee-Won;Hwang, Inseong;Kim, Jeong-Jin;Kim, Young-Hun
    • Journal of Environmental Science International
    • /
    • v.29 no.1
    • /
    • pp.69-77
    • /
    • 2020
  • Oxidative degradation of phenol, three monochlorophenols (2-chlorophenol, 2-CP; 3-chlorophenol, 3-CP; 4-chlorophenol, 4-CP), four dichlorophenols (2,3-dichlorophenol, 2,3-DCP; 2,4-dichlorophenol, 2,4-DCP; 2,5-dichlorophenol, 2,5-DCP; 2,6-dichlorophenol, 2,6-DCP), and two trichlorophenols (2,4,5-trichlorophenol, 2,4,5-TCP; 2,4,6-trichlorophenol, 2,4,6-TCP) was conducted with heat activated persulfate. As the number of chlorinations increased, the reaction rate also increased. The reaction rate was relatively well fitted to the zero-order kinetic model, rather than the pseudo-first order kinetic model for the reactions at 60 ℃, which can be explained by insufficient activation of the persulfate at 60 ℃, and the oxidation reaction of 2,4,6-TCP at 70 ℃ was relatively well fitted to the pseudo-first order kinetic model. The oxidation reaction rate generally increased with increase of persulfate concentration in the solution. 2,6-dichloro-2,5-cyclohexadiene-1,4-dione was found as a degradation product in a GC/MS analysis. This compound is a non-aromatic compound, and one chlorine was removed. This result is similar to the result of previous studies. The current study proved that heat activated persulfate activation could be an alternative remediation technology for phenol and chlorophenols in soil and groundwater.

Antibacterial, Anti-Diarrhoeal, Analgesic, Cytotoxic Activities, and GC-MS Profiling of Sonneratia apetala (Buch.-Ham.) Seed

  • Hossain, Sheikh Julfikar;Islam, M Rabiul;Pervin, Tahmina;Iftekharuzzaman, M;Hamdi, Omer AA;Mubassara, Sanzida;Saifuzzaman, M;Shilpi, Jamil Ahmad
    • Preventive Nutrition and Food Science
    • /
    • v.22 no.3
    • /
    • pp.157-165
    • /
    • 2017
  • Fruits of Sonneratia apetala (Buch.-Ham.), (English: mangrove apple, Bengali: keora) both seeds and pericarps, are largely consumed as food besides their enormous medicinal application. The fruit seeds have high content of nutrients and bioactive components. The seeds powder of S. apetala was successively fractionated using n-hexane, diethyl ether, chloroform, ethyl acetate, and methanol. The fractions were used to evaluate antibacterial, anti-diarrhoeal, analgesic, and cytotoxic activities. Methanol fraction of seeds (MeS) stronly inhibited Escherichia coli strains, Salmonella Paratyphi A, Salmonella Typhi, Shigella dysenteriae, and Staphylococcus aureus except Vibrio cholerae at $500{\mu}g/disc$. All the fractions strongly inhibited castor oil induced diarrhoeal episodes and onset time in mice at 500 mg extract/kg body weight (P<0.001). At the same concentration, MeS had the strongest inhibitory activity on diarrhoeal episodes, whereas the n-hexane fraction (HS) significantly (P<0.05) prolonged diarrhoeal onset time as compared to positive control. Similarly, HS (P<0.005) inhibited acetic acid induced writhing in mice at 500 mg extract/kg, more than any other fraction. HS and diethyl ether fractions of seed strongly increased reaction time of mice in hot plate test at 500 mg extract/kg. All the fractions showed strong cytotoxic effects in brine shrimp lethality tests. Gas chromatography-mass spectrometry analysis of HS led to the identification of 23 compounds. Linoleic acid (29.9%), palmitic acid (23.2%), ascorbyl palmitate (21.2%), and stearic acid (10.5%) were the major compounds in HS. These results suggest that seeds of S. apetala could be of great use as nutraceuticals.

Floral Nectary-specific Gene NTR1 Encodes a Jasmonic Acid Carboxyl Methyltransferase

  • Seo, Hak Soo;Song, Jong Tae;Koo, Yeon Jong;Jung, Choonkyun;Yeu, Song Yion;Kim, Minkyun;Song, Sang Ik;Lee, Jong Seob;Hwang, Ingyu;Cheong, Jong-Joo;Choi, Yang Do
    • Journal of Applied Biological Chemistry
    • /
    • v.44 no.3
    • /
    • pp.119-124
    • /
    • 2001
  • NTR1 gene of Brassica campestris L. ssp. perkinensis encodes a floral nectary-specific methyltransferase. In this study, the NTR1 cDNA was expressed in E. coli to examine the enzymatic characteristics of the protein product. The GST-NTR1 fusion protein was purified to near homogeneity, showing that the size of NTR1 was 44 kDa. The protein reacted specifically with jasmonic acid (JA), consuming methyl group from S-adenosyl-L-methionine (SAM). GC-MS analysis revealed that the compound produced was authentic methyl jasmonate (MeJA), suggesting that NTR1 is an S-adenosyl-L-methionine: jasmonic acid carboxyl methyltransferase. Km values of NTR1 for JA and SAM were 38.0 and $6.4{\mu}M$, respectively. Optimal activity of the NTR1 was observed at $20^{\circ}C$, pH 7.5, in the presence of 100-150 mM KCl. Thus, kinetic properties, thermal characteristics, optimal pH, and ion-dependency of the NTR1 activity were almost identical to those of Arabidopsis JA methyltransferase JMT, indicating that these two proteins are orthologues of each other.

  • PDF

The chloroform fraction of Citrus limon leaves inhibits human gastric cancer cell proliferation via induction of apoptosis

  • Osman, Ahmed;Moon, Jeong Yong;Hyun, Ho Bong;Kang, Hye Rim;Cho, Somi Kim
    • Journal of Applied Biological Chemistry
    • /
    • v.59 no.3
    • /
    • pp.207-213
    • /
    • 2016
  • Little information about the biological activities of Citrus limon (lemon) leaves has been reported, whereas the fruit of Citrus limon (lemon) has been well-documented to contain various pro-health bio-functional compounds. In the present study, the antiproliferative activities of the lemon leaves were evaluated using several cancer cell lines. From the n-hexane, chloroform, ethyl acetate, n-butanol, and water fractions of methanolic extract of the leaves, the chloroform fraction of lemon leaves (CFLL) showed the most potent antiproliferative activity in the AGS human gastric cancer cells. The current study demonstrates that CFLL induces apoptosis in AGS cells, as evidenced by an increase in apoptotic bodies, cell population in the sub-G1 phase, Bax/Bcl-2 ratio, and cleavage of poly (ADP-ribose) polymerase (PARP), caspase-3 and caspase-9. Compositional analysis of the CFLL using gas chromatography mass spectrometry (GC-MS) resulted in the identification of 27 compounds including trans, trans-farnesol (3.19 %), farnesol (3.26 %), vanillic acid (1.45 %), (-)-loliolide (5.24 %) and palmitic acid (6.96 %). Understanding the modes of action of these compounds individually and/or synergistically would provide useful information about their applications in cancer prevention and therapy.

Occurrence of Phthalates in Indoor Dust from Children's Facilities and Apartments in Seoul

  • Kim, Ho-Hyun;Lim, Young-Wook;Yang, Ji-Yeon;Shin, Dong-Chun
    • Journal of Korean Society for Atmospheric Environment
    • /
    • v.25 no.5
    • /
    • pp.382-391
    • /
    • 2009
  • The aim of the study was to assess the levels in typical central borough of phthalate exposure by monitoring children's facilities (19 kindergartens and 21 elementary schools) and households (17 old apartments and 22 new apartments) via sampling indoor floor dust. A vacuum cleaner specifically prepared for sampling dust was attached to a filter of a vacuum cleaner dust collecting container. During the sample preparation process, containers made of glass were used and analysis was performed using GC-MSD. The mean concentration of DEHP was $412{\mu}g/g$, DnBP was $24{\mu}g/g$, and BBzP was $10{\mu}g/g$ dust. The indoor characteristics including floor, wall materials, years after construction, water leakage history for the past three years, and ventilation were also examined to categorize phthalate esters from the dust samples. From the flooring and wallpaper materials of kindergartens and elementary schools, DEHP in the dust sample appeared at a statistically significant level (p<0.05) when PVC materials were present. DEHP in the indoor dust also increased significantly (p<0.01) during the construction period. The daily intake of DEHP measured from indoor dust was 0.08~$19.7{\mu}g/kg/day$ for children and 0.02~$1.1{\mu}g/kg/day$ for adults (women). The results clearly revealed that phthalate exposure is greater in children than in adults (women) due to the indoor dust.