• 한국미생물·생명공학회지
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• 제35권4호
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• pp.357-363
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• 2007

249 fungal strains were isolated from the roots of 26 plants, and the production of GAs was spectrophotometric ally examined. As a result 76 fungal strains were shown to produce GAs. Bioassay of culture broth from seventy six fungal strains producing GAs was carried out with waito-c rice, that is dwarf rice. The seventy six fungi with GAs-producing activity were incubated for seven days in 40 mL of Czapek's liquid medium at $30^{\circ}C$ and 180 rpm, and the culture broth of fungi were treated on the 2-leaf rice sprout. Fifteen of these showed plant growth promoting activity and the amount of each GAs in the medium was measured by Gas chromatographymass spectrometer (GC-MS). Nine of these fungi were also identified by genetic analysis of the nucleotide sequences in the internal transcribed spacer region of the ribosomal DNA.

• Journal of Microbiology and Biotechnology
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• 제22권9호
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• pp.1177-1184
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• 2012

In order to estimate how diverse the mating types in Pleurotus eryngii from different regions are, pairings between monokaryons derived from inter- and intra-groups were done. Sixteen and 15 alleles were identified at loci A and B from the 12 strains. In the P. eryngii KNR2312, widely used for commercial production, four mating loci, A3, A4, B3, and B4, were determined. Those loci, except A3, were found in 4 strains out of 12 strains. To improve breeding efficiency, especially in mating type determination, RAPD and BSA were performed to screen for a mating type specific marker. The SCAR marker 13-$2_{2100}$ was developed based on the RAPD-derived sequence typing B3 locus. The sequence analysis of 13-$2_{2100}$ revealed that it contained a conserved domain, the STE3 super-family, and consensus sequences like the TATA box and GC box. It seems likely that the SCAR marker region is a part of the pheromone receptor gene.

• Journal of Microbiology and Biotechnology
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• 제23권11호
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• pp.1598-1609
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• 2013

Organochlorine pesticide residues continue to remain as a major environmental threat worldwide. Lindane is an organochlorine pesticide widely used as an acaricide in medicine and agriculture. In the present study, a new lindane-degrading yeast strain, Pseudozyma VITJzN01, was identified as a copious producer of glycolipid biosurfactant. The glycolipid structure and type were elucidated by FTIR, NMR spectroscopy, and GC-MS analysis. The surface activity and stability of the glycolipid was analyzed. The glycolipids, characterized as mannosylerythritol lipids (MELs), exhibited excellent surface active properties and the surface tension of water was reduced to 29 mN/m. The glycolipid was stable over a wide range of pH, temperature, and salinity, showing a very low CMC of 25 mg/l. Bio-microemulsion of olive oil-in-water (O/W) was prepared using the purified biosurfactant without addition of any synthetic cosurfactants, for lindane solubilization and enhanced degradation assay in liquid and soil slurry. The O/W bio-microemulsions enhanced the solubility of lindane up to 40-folds. Degradation of lindane (700 mg/l) by VITJzN01 in liquid medium amended with bio-microemulsions was found to be enhanced by 36% in 2 days, compared with degradation in 12 days in the absence of bio-microemulsions. Lindane-spiked soil slurry incubated with bio-microemulsions also showed 20-40% enhanced degradation compared with the treatment with glycolipids or yeast alone. This is the first report on lindane degradation by Pseudozyma sp., and application of bio-microemulsions for enhanced lindane degradation. MEL-stabilized bio-microemulsions can serve as a potential tool for enhanced remediation of diverse lindane-contaminated environments.

• 한국미생물·생명공학회지
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• 제48권3호
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• pp.316-321
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• 2020

송이버섯이 첨가된 전통주를 제조한 후 송절편을 첨가하여 첨가량과 숙성 기간에 따른 향기 성분의 변화를 기체 크로마토그래피 질량분광법을 통해 조사하였다. 송절편을 첨가하지 않은 대조구에서는 총 3종의 향기 성분이 검출되었으나, 송절편을 첨가한 송이주에서는 총 15종의 향기 성분이 검출되었다. 송절편을 5% 첨가한 실험구에서 송이버섯 특유의 향기 성분인 β-fenchyl alcohol과 2-octanol, methyl cinnamate은 숙성기간 동안 상대적인 함량이 증가하여 8주 후에는 각각 67.57%, 2.14%와 0.94%를 나타냈다. 주성분 분석 결과 송절편 첨가에 의한 β-fenchyl alcohol의 상대적 함량 증가가 송이주의 향미 특성에 영향을 미치며, 송이주의 향미 특성은 송절편의 첨가량 보다 숙성 기간에 의해 더 큰 영향을 받는 것으로 판단된다.

• Journal of the Korean Wood Science and Technology
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• 제44권2호
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• pp.253-263
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• 2016

Fungi, such as the wood-rotting Polyporus brumalis, are excellent sources of pharmaceutically interesting natural products such as sesquiterpenoids. In this study, we investigated the biosynthesis of P. brumalis sesquiterpenoids on modified medium. Ten additional species of white rot fungi were inoculated in medium containing nutrients such as $C_6H_{12}O_6$, $C_4H_{12}N_2O_6$, $KH_2PO_4$, $MgSO_4$, and $CaCl_2$ at $28^{\circ}C$ for 25 days. After 10 days of incubation, eudesmane-type sesquiterpenes, ${\beta}$-eudesmane and ${\beta}$-eudesmol, were only synthesized during the growth phase of P. brumalis. Experiments excluding one nutrient at a time were conducted to determine the effects of inorganic nutrients on sesquiterpene biosynthesis. In conclusion, GC-MS analysis showed that biosynthesis of sesquiterpenes was differentially regulated by inorganic nutrients such as $MgSO_4$, $C_4H_{12}N_2O_6$, and $KH_2PO_4$. We found $MgSO_4$ supplementation to be vital for eudesmane-type sesquiterpene biosynthesis in P. brumalis; nitrogen ($C_4H_{12}N_2O_6$) and phosphate ($KH_2PO_4$) inhibited the synthesis of P. brumalis metabolites. Magnesium is a known cofactor of sesquiterpene synthase, which promotes ${\beta}$-eudesmol synthesis. To mechanistically understand eudesmane-type sesquiterpene biosynthesis in P. brumalis, further research into the genes regulating the dynamics of such biosynthesis is warranted.

• 한국환경과학회지
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• 제14권1호
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• pp.23-32
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• 2005

Surface sediments (0$\~$4 cm) were sampled at 40 stations from Ulsan Bay and its adjacent areas of Korea, to evaluate the contamination by sewage-derived organic matters using fecal sterols. Some sterols were quantified by gas chromatography coupled to mass spectrometry (GC/MS). Total concentrations of eight sterols (co­prostanol, cholesterol, cholestanol, epicholestanol, epicoprostanol, ${\beta}-sitosterol,$ brassicasterol, and stigmasterol) in the sediments from Ulsan Bay and its vicinity varied from 929 to 23,444 ng/g dry weight. The most predom­inant sterols were cholesterol and coprostanol, accounting for $33\~72{\%}$ of total sterols. The concentration of coprostanol known as the indicator of human feces ranged from 141 to 8,257 ng/g dry weight. In particular, the coprostanol concentrations in the sediments from Jansaengpo Harbor and Taehwa River exceeded the value of 1,000 ng/g dry weight, suggesting that these areas could be considered as the hot-spot zones by municipal sewage contamination. Some molecular indices and multivariate data analysis were used to assess the origin of these sterols in the sediments. Results showed that the major routes of sewage contamination in Ulsan Bay and its vicinity were the input through Jansaengpo Harbor and Taehwa River.

• 한국환경과학회지
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• 제17권7호
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• pp.767-774
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• 2008

This study was carried out to evaluate the characteristics of atmospheric concentrations of volatile organic compounds(VOCs) and aldehydes for near a large shipyard. Most of the painting work in marine coating is performed indoor and outdoor. Most of the VOCs are emitted to the atmosphere as the paint is applied and cures. The massive scale of a ship makes it difficult to capture the emissions from outdoor painting. The VOCs are an important health and contributors to photochemical smog. The VOCs and aldehydes samples were collected using adsorbent tube and 2,4-DNPH cartridge, and were determined by an automatic thermal desorption coupled with GC/MS and HPLC-UV analysis, respectively. A total of 16 aromatic VOCs and 12 aldehydes of environmental concern were determined. At indoor coating facilities, the most abundant compound among 16 target VOCs appeared to be m,p-xylene, being followed by o-xylene. But most of the aldehydes were extremely lower concentrations. The atmospheric concentration of VOCs, m,p-xylene concentrations were the highest and the mean value were outdoor workshop 11.323 ppb, residental area 5.134 ppb, and green area 2.137 ppb, respectively. However, the most aldehydes were extremely lower concentrations such as formaldehyde, acetaldehyde and non-detection such as iso-valeraldehyde, n-valeraldehyde and o-tolualdehyde.

• Journal of Forest and Environmental Science
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• 제33권2호
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• pp.113-121
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• 2017

In flight particulate matter particularly emissions generated by incomplete combustion processes has become a subject of global concern due to the health problems and environmental impacts associated with them. This has compelled most countries to set standards for coarse and fine particles due to their conspicuous impacts on environment and public health. This contribution therefore explores forest fire emissions and how its particulates affects air quality, damage to vegetation, water bodies and biological functions as architects for lung diseases and other degenerative illnesses such as oxidative stress and aging. Soot was collected from simulated forest fire using a clean glass surface and carefully transferred into amber vials for analysis. Volatile components of soot were collected over 10 mL dichloromethane and analyzed using a QTOF Premier-Water Corp Liquid Chromatography hyphenated to a mass selective detector (MSD), and Gas Chromatograph coupled to a mass spectrometer (GC-MS). To characterize the size and surface morphology of soot, a scanning electron microscope (SEM) was used. The characterization of molecular volatiles from simulated forest fire emissions revealed long chain compounds including octadec-9-enoic acid, octadec-6-enoic acid, cyclotetracosane, cyclotetradecane, and a few aromatic hydrocarbons (benzene and naphthalene). Special classes of organics (dibenzo-p-dioxin and 2H-benzopyran) were also detected as minor products. Dibenzo-p-dioxin for instance in chlorinated form is one of the deadliest environmental organic toxins. The average particulate size of emissions using SEM was found to be $11.51{\pm}4.91{\mu}m$. This study has shown that most of the emissions from simulated forest fire fall within $PM_{10}$ particulate size. The molecular by-products of forest fire and particulate emissions may be toxic to both human and natural ecosystems, and are possible precursors for various respiratory ailments and cancers. The burning of a forest by natural disasters or man-made fires results in the destruction of natural habitats and serious air pollution.

• Preventive Nutrition and Food Science
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• 제16권1호
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• pp.55-61
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• 2011

A survey aiming to find out the chemical and textural properties of commercial fermented soy bean curd called sufu was conducted. Sixteen brands of plain sufu produced in the Northern or the Southern part of China were collected and examined for their crude protein, crude fat, texture profiles, free amino acids, and free fatty acid contents. Twenty-one free amino acids were extracted and derivatized using a commercial kit followed by separation and analyzed by the gas chromatography-mass spectrometry (GC-MS). Similarly, ten free fatty acids were extracted using alumina, eluted, separated and analyzed. The content ranges of crude fat and protein were 22~36% and 31~38%, respectively. In texture profile analysis, ranges of the texture parameters were 131~493 g (hardness), 0.4~0.5 (cohesiveness), -137 to -50 gs (adhesiveness), 0.6~1 (springiness), 47~220 g (gumminess) and 32~177 g (chewiness). Twenty-one different free amino acids, especially alanine, glycine, $\alpha$-aminobutyric acid, valine, leucine, allo-isoleucine, aspartic acid, glutamic acid and lysine in large amount, as well as ten fatty acids in total, notably linoleic acid (9-octadecanoic acid), oleic acid (9,12-octadecadienoic acid), linolenic acid (9,12,15-octadecadienoic acid), hexadecanoic acid and octadecanoic acid were found. This information provides important quality reference ranges for product developers and manufacturers to optimize and produce the plain sufu.

• 한국전문물리치료학회지
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• 제2권2호
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• pp.1-8
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• 1995

이 논문의 주목적은 정상인을 대상으로 각기 다른 3가지의 (체중의 1.5 %, 3.0 %, 9.0 %) 부하를 통해서 자세의 불균형을 유발시켰을 때 나타나는 postural movement patterns을 기술하기 위한 연구이다. 연구대상의 허리중심에 체중부하를 주어 균형이 뒤로 이동하게 하여, surface EMG(표면 근전도)를 통하여 Tibialis anterior(Ta), Gastrocnernius(Gc), Quadriceps femoris(Qc), Hamstring(Ha), Rectus abdominalis(Ab)와 Paraspinalis(Pa) 근육들의 motor recruitment pattern(운동회집형태)를 측정하였다. 그리고 비디오 촬영은 고관절, 슬관절, 족관절의 움직임을 보기 위해 사용하였다. 특히 근전도 (EMG)는 자세반응 검사에 있어 첫 근반응(근수축) 경과시간(FR)과 분절간격간의 (ID)시간을 조사하는데 사용되었다. 이 연구의 결과는 4가지 중요한 사실을 전해주고 있다. 첫 번째로서, 연구대상자에게 체중의 1.5 %의 부하를 적용하였을 때 Ta가 가장 먼저 수축을 시작하였고(FR:$88{\pm}19.4$ ms) 발목과 대퇴사이의 분절 간격간(ID)의 평균은 +9.3 ms였다. 또한 족관절의 변화가 가장 뚜렷하여 Nashner(1985)의 족기전을 뒷받침하고 있다. 둘째로는 연구대상자에게 체중의 3.0 %의 부하를 주었을 때 Qc와 Ab근육이 원위부에서 근위부 순서로 수축하였고, 첫 번째 근육수축시간은 ($82{\pm}39.2$ ms)였다. 그리고 이때 분절간격의 평균은 +8.3ms 이였고 Ta는 거의 반응하지 않았다. 족관절과 슬관절에 비해 고관절의 변화가 가장 현저하게 나타났고, 이 또한 Nashner의 고관절 기전과 같은 현상을 보였다. 셋째로 연구 대상자에게 체중의 9.0 % 부하를 허리에 적용하였을 때 근수축은 근위부에서 원위부 순서를 이루어졌다. 즉 Ab,Qc, Ta, Ps순으로 근수축 되었다. Ab가 처음으로 수축하여 첫 반응(FR)은 $73{\pm}3.2$ ms 이였고 슬관절과 고관절의 변화가 가장 뚜렷하였다. 넷째로 연구 대상자에게 체중의 9.0 %부하를 적용하였을 때, 균형을 잡기 위해 뒷걸음치는 것이 관찰되었고 이때 근수축 순서는 Ta,Ab.Ps,Qc,Hs였다. 이 결과는 Nasher의 결과와 불일치하였다. 이상과 같은 결과에서 연구대상자의 자세운동형태(postural movement patterns)는 각기 다른 부하 정도와 시간에 따라 합성적으로 이루어지는 것으로 보여졌다. 특히, 자세운동형태는 부하의 적용위치와 연구대상자의 최근의 경험에 영향을 받은 것으로 밝혀졌다. 결론적으로 말하면 자세운동형태는 중앙신경계의 제한적(한정적) 명령 시스템에 의해서 움직임(movement)이 발생하기 전에 조직된다는 Nashner의 가설을 뒷받침하였다.