• Korean Journal of Acupuncture
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• v.19 no.1
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• pp.35-45
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• 2002

Acupuncture and herbal medicine have been used to prevent and treat the cerebrovascular accident, such as a stroke, and many studies of acupuncture and moxibustion concerning to the stroke have been undertaken in the human and various animals. However, it was not published the protective effect of the electroacupuncture (EA) of Huan-do (GB30) on the transient forebrain ischemia injury. The neuroprotective effects of EA (2 ms, 10 Hz, and 1 - 2 mA) of GB30 on the transient forebrain ischemia injury was investigated by western blot of nNOS and hematoxylin and eosin stain in Sprague-Dawley rats. The transient forebrain ischemia injury resulted in increased expression of nNOS in the brain for 6 hrs after ischemia, and EA decreased significantly expression of nNOS protein in brain increased by transient forebrain ischemia injury. The survived neuronal cell stained with hematoxylin and eosin (H&E) decreased in the hippocampus by the 7 days after ischemia comparing with the normal. Comparing with the normal, the survived neurons seriously decreased cell in the hippocampus after the injury. However, the proportion of survived neurons was increased in EA treatment. These results suggest that EA of GB30 have neuroprotective effects on transient forebrain ischemia injury.

• Korean Journal of Organic Agriculture
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• v.10 no.4
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• pp.37-45
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• 2002

This study was conduction to investigate the effects of natural materials such as $GB_{10}$ chitofarm, chaff charcoal, and chaff charcoal sap, on quality and yield of chinese cabbage, Natural materials were treated on seeds soil, and leaves. The total plant weight, head weight, head length, head width, leaf length, leaf width, and sugar content of chinese cabbage in treatment with natural materials showed significant difference compared with control. The yield of chinese cabbage in $GB_{10}$ soil treatment and 1% $GB_{10}$ leaf treatment was increased 53% compared with control. The total nitrogen $P_2O_5$, $K_2O$, CaO, MgO, $Na_2O$, and Mn in total plant were analyzed. The chemical components were increased in total plant of chinese cabbage treated with natural materials compared with control natural materials increased the quality of chinese cabbage.

• Journal of Korean Medicine Rehabilitation
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• v.29 no.1
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• pp.21-29
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• 2019

Objectives This study is designed to verify the effectiveness of electroacupuncture (EA) on both side acupuncture point GB12, GB20 for chronic tension type headache (CTTH). Methods Subjects aged 18-65 years who had suffered from CTTH for more than 3months were recruited from September 2016 to May 2017. Thirty subjects were voluntarily recruited and they were randomly divided into 2 groups; 15 to EA group, another 15 to sham-EA group. The treatment sessions were performed every 2-4 days for a total of three sessions over 1 week. The symptoms of headache were assessed before the treatment and after a week from the last treatment by visual analogue scale (VAS), headache frequency (HF), Korean Headache Impact Test-6 (KHIT-6). Demographic characteristics of all participants were compared between the groups upon using chi-square test. Wilcoxon signed rank test was used to evaluate the values between baseline and follow-up. Wilcoxon rank sum test was performed to evaluate the differences between groups. p-values less than 0.05 were considered as significant. Results Before and after the intervention, no difference in VAS and KHIT-6 was observed between groups. HF of EA group decreased significantly compared with that of sham-EA group. Within the EA group VAS, HF and KHIT-6 decreased significantly after the treatment. Meanwhile, only KHIT-6 decreased significantly after the treatment in sham-EA group. Conclusions This study suggests that EA on both side acupuncture point GB12 and GB20 is applicable to improve symptoms in patients with CTTH.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.22 no.6
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• pp.107-112
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• 2022

In an embedded system such as a drone, it is difficult to store, transfer and analyze the entire hyper-spectral image to a server in real time because it takes a lot of power and time. Therefore, the hyper-spectral image data is transmitted to the server through dimension reduction or compression pre-processing. Feature selection method are used to send only the bands for analysis purpose, and these algorithms usually take a lot of processing time depending on the size of the image, even though the efficiency is high. In this paper, by improving the temporal disadvantage of the band selection algorithm, the time taken 24 hours was reduced to around 60-180 seconds based on the 40000*682 image resolution of 8GB data, and the use of 7.6GB RAM was significantly reduced to 2.3GB using 45 out of 150 bands. However, in terms of pixel classification performance, more than 98% of analysis results were derived similarly to the previous one.

• Korean Journal of Exercise Nutrition
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• v.14 no.2
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• pp.63-68
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• 2010

This study evaluated the effects of high pressured red ginseng(HRG) and ginseng berry(GB) supplementation on lipid profiles and run to exercise time in SD rats. Five weeks-old male rats were randomly assigned into six groups, ie. control group(AC: n = 8), exercise group(AE: n = 7), general extracted red ginseng group(C: n = 8), HRG group(high density D: n = 8, low density E: n = 7), and HRG + GB group(F: n = 7). The AC and AE groups had a supplementation of AIN-93G, and AE group performed 40 min treadmill exercise five times per week for five weeks. E, C, D, and F groups had a supplementation composed of general extracted red ginseng(GRG), HRG(250 mpk and 500 mpk), and HRG(250 mpk) + GB(167 mpk) in the AIN-93G. Main results of the study were as follow 1) the duration of running time to exhaustion was higher in exercise group compared to other groups. HRG had no effect on run to exhaustion time, but, GRG and HRG + GB showed longer run time than control groups. 2) Blood TC, TG, HDL-C and liver TG showed no difference between groups, but, liver TC showed lower level in the HRG groups than other groups. It was concluded that the 5 weeks of supplementation of HRG extract would have positive effects on liver cholesterol, and GRG and HRG + GB have a positive effect on exercise performance tested run to exhaustion time, but, we can not find a positive effect of supplementation of HRG on exercise performance.

• Microbiology and Biotechnology Letters
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• v.38 no.3
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• pp.283-288
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• 2010

Yam (Dioscorea spp.) has been used as important edible and medicinal natural resource in worldwide and D. alata L. is most popular nourishment among the yam. In this study the composition, color, antioxidation and antimicrobial activity of D. alata Gyeongbuk No. 6 (GB-6), which was established in Gyeongbuk Agricultural Research & Extension Services, Andong, Korea, was compared to those of D. batatas Gyeongbuk No. 1 (GB-1), a major domestic cultivation species. Water content of GB-6 was $78.02{\pm}0.16%$, which is slightly lower than that of GB-1 ($82.6{\pm}0.07%$). The contents of crude protein, crude fat, crude fiber and ash of GB-6 were 0.95, 0.26, 0.85 and 0.70%, whereas those of GB-1 were 1.58, 0.15, 1.39 and 0.88%, respectively. Analysis of color using colormeter showed that the GB-6 is slight dark-yellow than GB-1, and total polyphenol content of GB-6 was 2-fold higher compared than that of GB-1. Sequential organic solvent fractions from methanol extract of GB-6 showed that the ethylacetate fraction has highest total polyphenol ($144.1{\pm}3.20\;mg/g$). Determination of antioxidation activity showed that the ethylacete fraction and water fraction has strong DPPH radical scavenging activity ($IC_{50}=78.32\;{\mu}g/mL$) and reducing power, respectively. In antimicrobial activity assay, the n-hexane and ethylacetate fraction showed antibacterial activity against B. subtilis, L.monocytogenes, S. epidermidis, S. aureus, P. vulgaris, and S. typhimurium. These results provide the possibility of domestic cultivated D. alata GB-6 as a healthy food.

• Journal of Veterinary Clinics
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• v.24 no.4
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• pp.608-612
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• 2007

A 4-year-old castrated male Cocker spaniel was referred with chief complaint of pelvic limbs paralysis and trembling. This dog was diagnosed as a case of myelomalacia complicated with intervertebral disc disease(IVDD) by radiography and magnetic resonance imaging(MRI). This patient was treated by injection-AP with apitoxin, herbal medicine and moxibustion. The acupoints such as GV02-1, GV04, GV11, GV12, GV13, GV14, BL11, BL18, BL23, BL25, BL28, GB21, HT07, KI03, LI04, LU07, SI03, SI06, ST36, ST38, ST39, ST40, ST41, GB30, GB31, GB34, Liv03, SP06 and trigger points($T1{\sim}T4\;and\;T9{\sim}L1$) were used for treatment. At the session 1, the dog could not stand and move the limbs. However, gradual improvement of clinical signs was observed after 7, 14 and 21 session of treatment. Although the clinical signs related to pelvic paralysis were not completely abolished with TVM methods, this case showed the marked improvement of clinical signs after 21 sessions of treatment. In conclusion, the present patient was a case with canine myelomalacia complicated with IVDD which showed not so complete but somewhat improvement by TVM method.

• Journal of Mushroom
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• v.15 no.2
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• pp.78-83
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• 2017

Microsatellite SSR markers were developed and utilized to reveal the genetic diversity of 32 strains of Flammulina velutipes collected in Korea, China, and Japan. From the SSR-enriched library, 490 white colonies were randomly selected and sequenced. Among the 490 sequenced clones, 85 (17.35%) were redundant. Among the remaining 405 unique clones, 201 (49.6%) contained microsatellite sequences. We used 12 primer pairs that produced reproducible polymorphic bands for four diverse strains, and these selected markers were further characterized in 32 Flammulina velutipes strains. A total of 34 alleles were detected using the 12 markers, with an average of 3.42 alleles, and the number of alleles ranged from two to seven per locus. The major allele frequency ranged from 0.42 (GB-FV-127) to 0.98 (GB-FV-166), and values for observed ($H_O$) and expected ($H_E$) heterozygosity ranged from 0.00 to 0.94 (mean = 0.18) and from 0.03 to 0.67 (mean = 0.32), respectively. SSR loci amplified with GB-FV-127 markers gave the highest polymorphism information content (PIC) of 0.61 and mean allele number of five, whereas for loci amplified with GB-FV-166 markers these values were the lowest, namely 0.03 and two. The mean PIC value (0.29) observed in the present study with average number of alleles (3.42). The genetic relationships among the 32 Flammulina velutipes strains on the basis of SSR data were investigated by UPGMA cluster analysis. In conclusion, we succeeded in developing 12 polymorphic SSRs markers from an SSR-enriched library of Flammulina velutipes. These SSRs are presently being used for phylogenetic analysis and evaluation of genetic variations. In future, these SSR markers will be used in clarifying taxonomic relationships among the Flammulina velutipes.

• Journal of Acupuncture Research
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• v.22 no.5
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• pp.67-77
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• 2005

Objectives : The purpose of this study is to examine if Bee Venom Acupuncture may be effective to the neuropathic pain(mechanical allodynia, cold allodynia) in a rat model of neuropathic pain. Methods : To produce the model of neuropathic pain, under isoflurane 2.5% anesthesia, tibial nerve and sural nerve was resected. After the neuropathic surgery, the author examined if the animals exhibited the behavioral signs of alloynia. The allodynia was assessed by stimulating the medial malleolus with von Frey filament and acetone. Three weeks after the neuropathic surgery, Bee Venom Acupuncture was injected at Hwando(GB30) one time a day for one week. After that, the author examined the withdrawl response of neuropathic rats' legs by yon Frey filament and acetone stimulation. And also the author examined c-Fos in the midbrain central gray of neuropathic rats and the change of WBC count in the blood of neuropathic rats. Results : The Bee Venom Acupuncture injected Hwando(GB30) decreased the withdrawl response of mechanical allodynia in BV-2, BV-3 group as compared with control group. The Bee Venom Acupuncture injected Hwando(GB30) decreased the withdrawl response of chemical allodynia(cold allodynia) in BV-2, BV-3 group as compared with control group. The Bee Venom Acupuncture injected Hwando(GB30) showed the significant difference between control group and BV-2 group, control group and BV-3 group in the c-Fos expression and U count. Conclusion : We have noticed that Bee Venom Acupuncture at Hwando(GB30) decreased mechanical allodynia and cold allodynia in the model of neuropathic pain compared with the control group. C-Fos expression in the central gray of that group was also decreased compared with the control group. Psin control using Bee Venom Acupuncture was accumulated as time goes by. This study can be used as a basic resource on a study and a treatment of pain.

• Journal of the korean academy of Pediatric Dentistry
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• v.36 no.3
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• pp.348-357
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• 2009

The purpose of this study was to evaluate the micro-tensile bond strength (${\mu}TBS$) of four luting resin to regional dentin of human primary teeth. Dentin from non-carious primary molars were prepared from different regions (s, superficial dentin; d, deep dentin; c, cervical dentin), and divided into groups based on anatomical locations and types of luting resins (Scotchbond Multi-purpose : SB ; One-Step : OS ; AdheSE Bond : ASE ; G-Bond : GB) : SB-s, SB-d, SB-c; OS-s, OS-d, OS-c; ASE-s, ASE-d, ASE-c ; GB-s, GB-d, GB-c. Luting resins were used according to the manufacturers' instructions, to bond $Light-Core^{TM}$ Core Build-Up Composite) to the exposed dentin specimens in the light-curing mode. After storage for 1 day, ${\mu}TBS$ was tested at a cross-head speed of 1 mm/min. Data were analyzed with T-test and two-way ANOVA. The bonding interface and fractography analyses were performed with SEM. The results were as follows : 1. ${\mu}TBS$ to superficial dentin was significantly higher than to deep dentin for SB(p<0.05). But there were no significant differences in regional ${\mu}TBS$ among OS, ASE, GB(p>0.05). 2. There were no significant differences in ${\mu}TBS$ to superficial dentin among each groups. But, in deep dentin, ${\mu}TBS$ of SB-d was significantly lower than those of OS-d, ASE-d, and GB-d(p<0.05). ${\mu}TBS$ of OS-d was significantly higher than those of GB-d(p<0.05), but there were no significant differences in ${\mu}TBS$ of ASEd. There were no significant differences among ${\mu}TBS$ of ASE-d, OS-d, and GB-d.