• Journal of Practical Agriculture & Fisheries Research
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• v.7 no.1
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• pp.55-63
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• 2005

Tuber yield and content of general component and diosgenin which is a main bioactive property were investigated in order to determine the growth characteristics of round typed yam(Dungeun-ma, Dioscorea opposita L.) and the potential of artificial culture at Suwon, Korea. Sprouting rates of segmented small tubers such as 3, 5, 7, 9, and 12g were respectively 60.3, 80.5, 85.3, 98.6 and 99.3% when they were investigated 30 days after planting. Segmented small tubers of 3, 5, 7, 9, and 12g enlarged respectively to 66, 87, 98, 120, and 140g in the green-house cultivation. Segmented small tubers of 3, 7, 12, 18, and 23g enlarged respectively to 35.2, 124.7, 142.7, 174.8, and 200,7g in the open-air field cultivation.

• BMB Reports
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• v.46 no.12
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• pp.611-616
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• 2013

Luteolin-7-O-glucoside (LUT7G), a flavone subclass of flavonoids, has been found to increase anti-oxidant and anti-inflammatory activity, as well as cytotoxic effects. However, the mechanism of how LUT7G induces apoptosis and regulates cell cycles remains poorly understood. In this study, we examined the effects of LUT7G on the growth inhibition of tumors, cell cycle arrest, induction of ROS generation, and the involved signaling pathway in human hepatocarcinoma HepG2 cells. The proliferation of HepG2 cells was decreased by LUT7G in a dose-dependent manner. The growth inhibition was due primarily to the G2/M phase arrest and ROS generation. Moreover, the phosphorylation of JNK was increased by LUT7G. These results suggest that the anti-proliferative effect of LUT7G on HepG2 is associated with G2/M phase cell cycle arrest by JNK activation.

• Journal of Environmental Science International
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• v.12 no.7
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• pp.689-695
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• 2003

The purpose of this study is to evaluate and to predict of eutrophication in lakes by using Vollenweider-OECD model and total phosphorus concentration and inflow rate which were measured in 1993∼2001. The results of study were as follows. The annual total phosphorus loading from the watershed was calculated to be 55∼195tP/yr at lake Soyang, 221∼466tP/yr at lake Taechong, 123∼278tP/yr at lake Andong, 57∼109tP/yr at lake Seomjin. These are discharged, far the most parts, from population and fishfarm facility. TP loading on the surface area at lake Soyang was 3.01gP/㎡/yr, 2.82gP/㎡/yr, 2.84gP/㎡/yr, 3.03gP/㎡/yr, 2.34gP/㎡/yr, 1.78gP/㎡/yr, 0.91gP/㎡/yr, 0.89gP/㎡/yr, 0.86gP/㎡/yr, lake Taechong was 6.71gP/㎡/yr, 7.25gP/㎡/yr, 7.24gP/㎡/yr, 6.53gP/㎡/yr, 6.50gP/㎡/yr, 7.06gP/㎡/yr, 7.04gP/㎡/yr, 4.05gP/㎡/yr, 3.44gP/㎡/yr and TP loading on the surface area of lake Andong, lake Soemjin were 5.39gP/㎡/yr, 4.47gP/㎡/yr, 4.56gP/㎡/yr, 4.45gP/㎡/yr, 3.33gP/㎡/yr, 2.38gP/㎡/yr, 2.53gP/㎡/yr, 2.46gP/㎡/yr, 2.54gP/㎡/yr, 4.09gP/㎡/yr, 4.10gP/㎡/yr, 3.98gP/㎡/yr, 3.73gP/㎡/yr, 2.80gP/㎡/yr, 3.46gP/㎡/yr, 3.22gP/㎡/yr, 2.19gP/㎡/yr, 2.13gP/㎡/yr respectively. The tropic states of four lakes can be assessed as eutrophy because phosphorus leading exceeds the critical phosphorus loading by Vollenweider-OECD model.

• Pediatric Infection and Vaccine
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• v.11 no.1
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• pp.59-72
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• 2004

Purpose : Rotaviruses are the major cause of gastroenteritis in infants and young children worldwide. It is important to get the epidemiologic data of rotavirus genotype for the application of rotavirus vaccine. So we tried to investigate the distribution of rotavirus genotypes with RT-PCR. Methods : A total of 120 rotavirus latex agglutinin test positive stool samples were collected continually from 120 children from Sep. 2000 to Apr. 2001. Rotavirus P(VP4), G (VP7) genotypes were determined by RT-PCR. Results : The genotype was identified in 116 stool samples of total 120 samples(96%). The incidence of G genotype was as follow; G1 17(14.2%), G2 74(61.7%), G4 1(0.8%), G9 1(0.8%). There were four cases of multiple genotypes; G1/G2, G1/G4, G1/G9, G8/G9 and genotype of G3, G8 were not found. Twenty three(19.2%) samples were nontypeable. The incidence of P was as follow; P[4] 77(64.2%), P[6] 22(18.3%), P4/P6 12(10%), P[4]/P[8] 1(0.8%) p[8] 1(0.8%). Seven(5.9%) samples were nontypeable. Conclusion : Various combinations of G and P genotypes were observed. Most rotavirus strains were P[4]G2 62(51.74%), followed by P[6]G2 7(5.8%), and P[6]G1 7(5.8%), P[4/P[6] G1 4(3%), P[4]/P[6]G2 4(3%), P[4]G1 3(2.5%), P[8]G2 1(0.8%), P[4]G4 1(0.8%) in Kyoungsangnamdo, Korea during 2000~2001.

• Journal of the Korean Graphic Arts Communication Society
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• v.31 no.1
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• pp.65-78
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• 2013

In gravure printing ink in printed flexible packaging considering the characteristics of the study more accurate color management options and results were as follows. When applying G7 calibration, P2P of the target GRACol G7 evaluate the delta $L^*$ and delta $F^*$, CMY and K-scale average of the results of delta $L^*$ and the highest tolerance G7 average of 1.5 and 3.0 are included in all the best. In addition, the average delta $F^*$ and super delta $F^*$, G7 tolerance by being included within the scope of color management, G7 calibration was possible. Target IT 8.7/4 CMYK, when applied to the calibration G7, Color gravure printing machine is applied to the average of the previous decreased from 12.4 to 3.6. In addition, if a digital proof is EPSON WT 7900 the average color applied to the previous reduction from 5.24 to 0.74 because of the gravure printing color proofing system was effective in the management. G7 calibration by applying the reference print profile of the Epson WT 7900-G-icc, the average was 0.74 coloration, and gravure-G-icc cases, the average color of the 3.60 per GRACol average of all the five colors below were included within the allowable range. Thus, the flexible packaging gravure printing color management of printed after applying the first G7 calibration, the results refer to the press by the profiling, and where best to take advantage of the profile creation was good.

• Journal of Ginseng Research
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• v.37 no.2
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• pp.201-209
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• 2013

Ginsenoside, one of the active ingredients of Panax ginseng, has a variety of physiologic and pharmacologic effects. The purpose of this study was to explore the effects of ginsenoside Rd (G-Rd) on melastatin type transient receptor potential 7 (TRPM7) channels with respect to the proliferation and survival of AGS and MCF-7 cells (a gastric and a breast cancer cell line, respectively). AGS and MCF-7 cells were treated with different concentrations of G-Rd, and caspase-3 activities, mitochondrial depolarizations, and sub-G1 fractions were analyzed to determine if cell death occurred by apoptosis. In addition, human embryonic kidney (HEK) 293 cells overexpressing TRPM7 channels were used to confirm the role of TRPM7 channels. G-Rd inhibited the proliferation and survival of AGS and MCF-7 cells and enhanced caspase-3 activity, mitochondrial depolarization, and sub-G1 populations. In addition, G-Rd inhibited TRPM7-like currents in AGS and MCF-7 cells and in TRPM7 channel overexpressing HEK 293 cells, as determined by whole cell voltage-clamp recordings. Furthermore, TRPM7 overexpression in HEK 293 cells promoted G-Rd induced cell death. These findings suggest that G-Rd inhibits the proliferation and survival of gastric and breast cancer cells by inhibiting TRPM7 channel activity.

• Microbiology and Biotechnology Letters
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• v.23 no.5
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• pp.556-558
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• 1995

A strain which showed cephalosporin C resistance and 7-aminocephalosporanic acid sensitivity was isolated from nature. Among the isolates, SS5 was sensitive to cephalosporin C, penicillin G, ampicillin, 7-aminocephalosporanic acid, 6-aminopenicillanic acid, and 7-aminodeacetoxy cephatosporanic acid at concentrations of 1,000 $\mu $g/ml, 2,000 $\mu $g/ml, 3,000 $\mu $g/ml, 30 $\mu $g/ml 100 $\mu $g/ml and 100 $\mu $g/ml, respectively. But SS5 was sensitive at very low concentration of chloramphenicol, kanamycin, neomycin, streptomycin and tetracycline. Since SS5 was sensitive to 7-ACA (30 $\mu $g/ml) and didn't have $\beta $-lactamase activity on the cephalosporin C, SS5 could be useful as an indicator strain for the production of 7-ACA, which is an important precursor for the synthesis of many semisynthetic cephalosporins.

• Analytical Science and Technology
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• v.18 no.3
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• pp.224-231
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• 2005

In this study, the optimum conditions for the LPME (liquid phase microextraction) were investigated to overcome several shortcomings of traditional liquid-liquid extraction method. The LPME, which is automatic and dynamic, was used to analyze the five pesticides (dementon-S-methyl, diazinon, parathion, fenitrothion, EPN) extracted from vegetable, and HP 6890 GC/NPD was used as an analytic instrument. It was possible to optimize the extraction condition using the automatic LPME. The optimum extraction rate was obtained at pH 3.0 and $100{\mu}g/mL$ of salt concentration and standard curve showed linearity with over $R^2=0.9921$ in the range of $0.2{\sim}10{\mu}g/g$. The relative standard deviations were 7.7%, 9.8%, 7.8%, 9.7% and 8.2% in the $5.0{\mu}g/g$ concentration of dementon-S-methyl, diazinon, parathion, fenitrothion and EPN, respectively. The acquired accuracies were satisfactory showing 12.7%, 7.8%, 10.4%, -6.7% and -0.7% for dementon-S-methyl, diazinon, parathion, fenitrothion and EPN respectively.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.9 no.2
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• pp.147-152
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• 2006

Purpose: Efficacy of the new rotavirus vaccines ($Rotarix^{(R)}$, $RotaTeq^{(R)}$) recently developed can be affected by the rotavirus genotypes prevalent in communities. We performed this study to identify the recent distribution of rotavirus genotypes prevalent in Jeju. Methods: Genotyping of human rotaviruses was performed using 81 samples collected from 154 inpatients and outpatients with rotavirus gastroenteritis at Cheju National University Hospital between July 2005 and June 2006. All six (1, 2, 3, 4, 8, 9) G serotypes were identified by amplification of segments of the gene for VP7 using the reverse transcription-polymerase reaction (RT-PCR). Results: The results of RT-PCR for 81 samples were all positive. G typing of the VP7 protein showed that G1 was the most dominant circulating genotype (65.5%) followed by G2 (14.8%), G3 (13.6%), G8 (1.2%), G9 (1.2%), G4 (0%), and a combination of G1/G3 (3.7%). Conclusion: This distribution of rotavirus VP7 genotypes in Jeju is different from that in other domestic areas; the most dominant circulating genotype was G1.

• Journal of Nutrition and Health
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• v.23 no.2
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• pp.124-134
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• 1990

To investigate nutritional and growth status of premature infants, survey was conducted against 19 premature infants admitted to Nursery of Pediatrics, Young nam University Hospital from Jan. 1984 to July 1988. Variations in feeding patterns as analyzed through the five feeding phases of dextrose, dextrose/hyperal, dextrose/formula, dextrose/hyperal/formula and formula. During 30days from birth, dietary intake, feeding phase, selective anthropometric measurements, biochemical parameters and demograpic information of premature infants were as follows: Distributions of birth weight by gestational age and apgar score showed preamture infants below 1499g were 31 weeks, 4/7(1min/5min) and premature infants from 1500g to 1999g were 33 weeks, 8/9(1min/5min) and premature infants from 2000g to 2500g were 33 weeks, 8/9(1min/5min). Average calorie intakes of premature infants below 1499g, premature infants from 1500g to 1999g and premature infants from 2000g to 2500g were 102.0㎉/kg/day, 119.3㎉/kg/day and 101.7㎉/kg/day. The mean values Na, K, Ca, P for all premature infants remained within the normal level for full term infants throughtout the 25days of this study period. Percentages of inital weight loss showned premature infants below 1499g were 5.4%, premature infants from 1500g to 1999g were 6.4%, premature infants from 2000g to 2500g were 11.4%. Percentages of inital weight loss of the amino acid injection group and the amino acid control group were 9.4% and 9.0%.