• Title/Summary/Keyword: G19

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Simultaneous Analysis Method for Polar and Non-polar Ginsenosides in Cultivated Wild Ginseng by Reversed-phase HPLC-CAD (HPLC-CAD에 의한 산양삼의 극성 및 비극성 ginsenoside 동시 분석)

  • Ok, Seon;Kang, Jae Seon;Kim, Kang Min
    • Journal of Life Science
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    • v.26 no.2
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    • pp.247-252
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    • 2016
  • Cultivated wild ginseng is a widely used dietary supplement and medicinal herb. The aim of this study was to optimize the ginseng using high performance liquid chromatography (HPLC)- charged aerosol detection (CAD) for ginsenoside analysis. CAD measures the physical property of an analyte and responds to almost all non-volatile species, independent of their nature, spectral properties, or particle size. It has become widely employed in pharmaceutical analysis. The cultivated wild ginseng extracts were analyzed for compositions of ginsenosides Rb1, Rd, Rg1, Rf, Re, and Rh1. The optimal analysis condition was set up from an experiment using a gradient. Ten grams of cultivated wild ginseng were extracted with 95% EtOH 100 ml for 24 hr at 80℃. The contents of the 6six major ginsenosides in the cultivated wild ginseng extract were Rb1 (5.48±0.12 mg/g), Rd (5.33±0.14 mg/g), Rg1 (12.80± 0.05 mg/g), Rf (19.08±0.68 mg/g), Re (19.87±0.05 mg/g), and Rh1 (16.47±0.16 mg/g), respectively. HPLC showed that the protopanaxatriol group (Rg1, Rf, Re, Rh1) had more content than the protopanaxadiol group (Rb1, Rd) in cultivated wild ginseng extract. In summary, the ginsenosides were identified with HPLC-CAD analysis, and their presence and quantity imply the importance of quality control, as well as the pharmacological activity of the ginseng root.

Effects of Crude Protein Levels on Nitrogen Balance in Adult Jindo Dog (사료내 조단백질 수준이 진도성견의 질소균형에 미치는 영향)

  • Kang, Tae-Il;Maeng, Won-Jai;Kim, Myeong-Hwa;Lee, Sang-Rak
    • Journal of Animal Science and Technology
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    • v.51 no.2
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    • pp.155-162
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    • 2009
  • This study was conducted to determine the protein requirement level in adult Jindo dog (Korea Jindo Dog) through nitrogen balance experiments. 12 female dogs aged 36~38 weeks old (for early stage) or aged 45~49 weeks old (for late stage) were fed one of 3 diets containing 17, 19 and 21% of crude protein. Body weight of dogs were measured during the 12 days of feeding trial, and feces and urine were collected last 3 days of the trial period. In early stage of Jindo dog, average daily gain of dogs fed experimental diets containing 17, 19 and 21% of crude protein were -38.33, -16.25 and -12.71g/d, respectively. There was a significant differences between 17 and 21% of crude protein treatments (p<0.05) and calculated retained nitrogen were -0.29, -0.04 and -0.03g/kg$BW^{.75}$/d, respectively. In late stage of Jindo dog, average daily gain of dogs fed experimental diets containing 17, 19 and 21% of crude protein were -34.05, -28.71 and -28.28g/d, and calculated retained nitrogen were -0.33, -0.06 and 0.09g/kg$BW^{.75}$/d, respectively, and were significantly higher (p<0.05) in dogs fed 21% of crude protein diet than those of other dogs. In early stage and late stage of adult Jindo dog, a calculated linear regression equation for nitrogen intake (x) and nitrogen retention (y) were y = 0.7484x-1.18 ($R^2$ = 0.9923) and y = 0.88231x-1.0894 ($R^2$ = 0.9982) and the requirement of crude protein were estimated as 9.85g/kg$BW^{.75}$/d and 7.72g/kg$BW^{.75}$/d from above equation.

Bioactive Effects of Domestic Cherry Tomatoes (국내산 방울토마토의 생리활성 효과)

  • Choi, Suk-Hyun
    • The Journal of the Korea Contents Association
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    • v.22 no.3
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    • pp.567-575
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    • 2022
  • This study was carried out to elucidate bioacive effects of three domestic cherry tomato cultivars. Total polyphenol and flavonoid of Summerking, Qutiquti, and Minichal cultivar were 12.56±1.88, 12.50±1.92, 11.65±1.85 mg/g and 4.58±1.03, 4.19±0.40, 4.30±0.49 mg/g(dry weight) respectively. Domestic cherry tomatoes showed antioxidative activity(DPPH and ABTS radical scavenging activities). All of the cherry tomatoes had no cytotoxicity for normal liver cell, but showed strong inhibitory effect against cervical cancer cell(HeLa) growth. These results revealed that domestic cherry tomatoes can be used as a bioactive food material.

Food component characteristics and antioxidant activities of commercial shiitake mushrooms (Lentinula edodes) (시판 표고의 식품성분 특성 및 항산화 활성)

  • Lee, Soo-Jung;Ryu, Ji-Hyeon;Kim, In-Soo
    • Journal of Mushroom
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    • v.19 no.3
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    • pp.216-224
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    • 2021
  • This study analyzed food component characteristics and antioxidant activities in five kinds of domestic commercial shiitake mushrooms (Lentinula edodes). Moisture content (79.17-82.90 g/100 g) showed little difference, but there was a significant difference in crude protein content. Total mineral content showed a significant difference, and potassium (K) content was the highest. Total and essential amino acid contents were higher in mushroom A than in the other samples. Nonetheless, the ratios of essential amino acids, aspartic acid, and glutamic acid to total amino acids were similar in all samples. 𝛽-Glucan content was in the range of 10.28-5.68 g/100 g, exhibiting a significant difference. The color intensity and overall texture of the mushrooms showed no remarkable difference. Total phenol and flavonoid contents were in the range of 19.92-30.77 mg/100 g and 6.95-10.39 mg/100 g, respectively; the ratio of flavonoids to total phenols ranged from 31.53% to 38.21%. DPPH and ABTS radical scavenging activities were 74.92-79.04% and 80.47-84.97%, respectively, and showed little difference. However, the reducing power varied between 195.23 𝜇M and 317.85 𝜇M, displaying a significant difference in all samples. Therefore, this study observed similar trends in the food component characteristics and antioxidant activities of domestic commercial shiitake mushrooms.

Different chemical and dynamical environments in two massive star forming regions, G19.61-0.23 and G75.78+0.34

  • Baek, Giseon;Lee, Jeong-eun;Cho, Se-Hyung;Yun, Youngjoo
    • The Bulletin of The Korean Astronomical Society
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    • v.42 no.1
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    • pp.55.4-56
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    • 2017
  • Complex organic molecules (COMs) are commonly detected in star forming regions and considered important species since they are seeds of prebiotic molecules. Although COMs form in ice mantles on dust grains, they are preferentially detected in the gas phase. The origin of the gaseous COMs highly depends on the circumstance of each star forming region. Therefore, the distribution of COMs emission reflects the physical and chemical conditions of the region. We present the newly detected COMs, especially methanol emission lines toward two massive star forming regions, G19.61-0.23 and G75.78+0.34 in the Atacama Large Millimeter/submillimeter Array Band 3 observations during the Cycle 2 phase. Multiple transitions of methanol are detected in both regions but show different emission morphology. The origin of the desorption (e.g. shocks or high energy photons) is discussed.

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