• Title/Summary/Keyword: G19

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Isolation and Identification of Lactic Acid Bacteria for Preparation of Probiotics (생균제용 유산균의 분리 및 동정)

  • 이진규;김운태;이준호;유재홍;신원철
    • Microbiology and Biotechnology Letters
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    • v.19 no.5
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    • pp.429-432
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    • 1991
  • In order to prepare probiotics, a strain which has resistant against acid, bile salts and antibiotics was isolated from infant and anirnat feces. Among these isolated strains, TK- 19 showed a strong acid tolerance and growth at 500 $\mu g$/ml of Na-deoxycholate. Also strain TK-19 was resistant to kanarnycin (30 $\mu g$/ml), streptomycin (10 $\mu g$/ml), nisin (50 $\mu g$/ml) and tetracycline (10 $\mu g$/ml). The isolated strain TK-19 was identified as Streptococcus faeciam, and was named Streptococcus faeciurn TK-19.

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Effects of Cholic Acid/CDCA and FGF-19 on the Protein Levels of the Endogenous Small Heterodimer Partner (SHP) in the Mouse Liver and HepG2 Cells (생쥐의 간과 HepG2 세포에 있어서 내인성 small heterodimer partner (SHP)의 단백질 수준에 미치는 cholic acid/CDCA 및 FGF-19의 효과)

  • Min, Gye-Sik
    • Journal of Life Science
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    • v.19 no.12
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    • pp.1731-1736
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    • 2009
  • Recent studies determined that a chronic western-style diet increased the endogenous small heterodimer partner (SHP) protein levels in mice. In experiments with cell cultures, chenodeoxy cholic acid (CDCA) treatment increased endogenous SHP protein levels and reduced the degradation rate of exogenously expressed flag-SHP levels in the human hepatoma cell line, HepG2 cells. In addition, bile acid-induced intestinal fibroblast growth factor-19 (FGF-19) increased the half-life of the exogenously expressed SHP when HepG2 cells were transfected with ad-flag-SHP. However, both the expression level and the degradation rate of the endogenous SHP in response to cholic acid and FGF-19 have not been well understood, either in mice or in cultured HepG2 cells. This study examined the effects of cholic acid treatment on the endogenous SHP protein levels in mice and the effects of FGF-19 on the degradation rate of the endogenous SHP protein in HepG2 cells. Mice fed 0.5% cholic acid in normal chow showed an increase in endogenous SHP protein levels during both 12 hr and 24 hr treatment periods as compared to control mice fed only normal chow. In cultured HepG2 cells, treatment with CDCA did not noticeably change the rate of degradation in the endogenous SHP protein from cells not treated with CDCA. Although consistent with the previous studies on the exogenous ad-flag-SHP protein, treatment with FGF-19 significantly decreased the degradation rate of the endogenous SHP protein when HepG2 cells were treated with cyclohexamide. These results suggest that both bile acids and FGF-19 increase the endogenous SHP protein levels in mouse liver and HepG2 cells.

Pseudomonas sp. G19 Alleviates Salt Stress and Promotes Growth of Chinese Cabbage (Pseudomonas sp. G19에 의한 배추의 염 스트레스 경감 및 생장 촉진)

  • Lee, Gun Woong;Lee, Kui-Jae;Chae, Jong-Chan
    • Korean Journal of Microbiology
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    • v.50 no.4
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    • pp.368-371
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    • 2014
  • A variety of abiotic stresses limit plant growth and crop productivity. Among the abiotic stress, salinity is one of the major harmful stresses to plants. Plant growth-promoting bacterium was isolated from reclaimed land soil of Kyehwa-do and identified as Pseudomonas. Pseudomonas sp. strain G19 produced $7.5{\mu}g/ml$ of indole acetic acid and solubilized 25% of insoluble phosphate after 36 h cultivation. Also, G19 was able to produce a protein that was structurally homologous to 1-aminocyclopropane-1-carboxylate deaminase of Pseudomonas fluorescens KACC10070 playing a role in reduction of ethylene in plant. The strain G19 increased the biomass of Chinese cabbage seedlings grown in the presence of 150 mM NaCl. The results indicated that the strain G19 promoted the growth of Chinese cabbage seedling under salinity stress through microbe-plant interactions.

Extablishment of Optimum Recipe on Persimmon Chokochujang using Persimmon Vinegar and Kochujang (감식초 및 감고추장을 이용한 감초고추장의 최적 배합비 설정)

  • 이기동;정용진;서지형;이명희
    • Journal of the East Asian Society of Dietary Life
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    • v.8 no.3
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    • pp.309-315
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    • 1998
  • The optimum recipe of persimmon chokochujang using persimmon vinegar and kochujang was established by four-dimensional response surface methodology. The organoleptic color of persimmon chokochujang showed maximum score in 18.16g of persimmon vinegar, 5.58g of garlic and 1.62 g of sucrose. The organoleptic aroma of persimmon chokochujang showed macimum score in 18.19g of persimmon vinegar, 4.57g of garlic and 2.06g of sucrose. The organoleptic taste of persimmon chokochujang showed maximum score in 19.28g of persimmon vinegar, 4.97g of garlic and 2.34g of sucrose. The organoleptic color of persimmon chokochujang showed maximum score in 18.81g of persimmon vinegar, 4.81g of garlic and 2.65g of sucrose. Optimum mixing ranges for organoleptic properties of persimmon chokkochujang were 18.25~19.25g of persimmon vinegar, 4.60~5.00g of garlic and 2.06~2.65g of sucrose.

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Comparison of Genetic Profiles and Prognosis of High-Grade Gliomas Using Quantitative and Qualitative MRI Features: A Focus on G3 Gliomas

  • Eun Kyoung Hong;Seung Hong Choi;Dong Jae Shin;Sang Won Jo;Roh-Eul Yoo;Koung Mi Kang;Tae Jin Yun;Ji-hoon Kim;Chul-Ho Sohn;Sung-Hye Park;Jae-Kyoung Won;Tae Min Kim;Chul-Kee Park;Il Han Kim;Soon-Tae Lee
    • Korean Journal of Radiology
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    • v.22 no.2
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    • pp.233-242
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    • 2021
  • Objective: To evaluate the association of MRI features with the major genomic profiles and prognosis of World Health Organization grade III (G3) gliomas compared with those of glioblastomas (GBMs). Materials and Methods: We enrolled 76 G3 glioma and 155 GBM patients with pathologically confirmed disease who had pretreatment brain MRI and major genetic information of tumors. Qualitative and quantitative imaging features, including volumetrics and histogram parameters, such as normalized cerebral blood volume (nCBV), cerebral blood flow (nCBF), and apparent diffusion coefficient (nADC) were evaluated. The G3 gliomas were divided into three groups for the analysis: with this isocitrate dehydrogenase (IDH)-mutation, IDH mutation and a chromosome arm 1p/19q-codeleted (IDHmut1p/19qdel), IDH mutation, 1p/19q-nondeleted (IDHmut1p/19qnondel), and IDH wildtype (IDHwt). A prediction model for the genetic profiles of G3 gliomas was developed and validated on a separate cohort. Both the quantitative and qualitative imaging parameters and progression-free survival (PFS) of G3 gliomas were compared and survival analysis was performed. Moreover, the imaging parameters and PFS between IDHwt G3 gliomas and GBMs were compared. Results: IDHmut G3 gliomas showed a larger volume (p = 0.017), lower nCBF (p = 0.048), and higher nADC (p = 0.007) than IDHwt. Between the IDHmut tumors, IDHmut1p/19qdel G3 gliomas had higher nCBV (p = 0.024) and lower nADC (p = 0.002) than IDHmut1p/19qnondel G3 gliomas. Moreover, IDHmut1p/19qdel tumors had the best prognosis and IDHwt tumors had the worst prognosis among G3 gliomas (p < 0.001). PFS was significantly associated with the 95th percentile values of nCBV and nCBF in G3 gliomas. There was no significant difference in neither PFS nor imaging features between IDHwt G3 gliomas and IDHwt GBMs. Conclusion: We found significant differences in MRI features, including volumetrics, CBV, and ADC, in G3 gliomas, according to IDH mutation and 1p/19q codeletion status, which can be utilized for the prediction of genomic profiles and the prognosis of G3 glioma patients. The MRI signatures and prognosis of IDHwt G3 gliomas tend to follow those of IDHwt GBMs.

Analysis for 16 Polynuclear Aromatic Hydrocarbons (PAH) in Sewage Sludge and Soil (하수슬러지와 토양 중 다핵방향족 탄화수소의 정량)

  • Kim, Jong-Hun
    • Analytical Science and Technology
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    • v.13 no.3
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    • pp.357-367
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    • 2000
  • The polycyclic aromatic hydrocarbon (PAH) content in sewage sludge and in farm soils were determined by gas chromatography linked to mass spectrometry (GC/MS) with use 2-ethylantracene as internal standards. Twelve PAH were identified in both sludges with naphthalene ($0.78{\mu}g/g$) being the most predominant in industrial sludge and pyrene ($0.26{\mu}g/g$) in municipal sludge. The total PAR content in industrial sludge and in municipal sludge were $1.74{\mu}g/g$ and $1.19{\mu}g/g$ respectively. PAH were contained in paddy soils and the concentration were very low in the range of $0.01-0.04{\mu}g/g$. The total PAH content in industrial and in municipal sludge were about 9.2 times and 6.3 times greater than in paddy soils ($0.19{\mu}g/g$).

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Patent Application Research Analysis on Domestic Smart Factory Technology Through SNA (SNA를 통한 국내 스마트공장 기술에 관한 특허 출원 조사 분석)

  • Jae-Hyo Hwang;Ki-Jung Kim
    • The Journal of the Korea institute of electronic communication sciences
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    • v.19 no.1
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    • pp.267-274
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    • 2024
  • In this paper, we investigated the number of domestic patent applications by year, the number of domestic patent disclosures by year, and the number of domestic registrations by year regarding smart factories. The number of patent applications by applicant type was investigated. Based on the patents studied, it was found that the IPC appearing in the most patents was G05B 19/418. In addition, through social network analysis of smart factory patented IPCs, it was found that G05B 19/418 was the IPC with the highest degree of centrality. From the above, if the IPC of the core technology of the patent submitted for smart factory is G05B 19/418, the technology combined with G05B 23/02, that is, the technology combining "factory control" and "monitoring" is the most patented. When the IPC of the core technology was G06Q 50/04, it was confirmed that the technology combined with G06Q 50/10, that is, the technology combining "manufacturing" and "service" was the most applied for patents. Through this, it was found that in order to apply for a patent for a smart factory, it would be necessary to file a patent application that takes into account the connectivity between IPCs.