• 대한의생명과학회지
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• 제6권4호
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• pp.261-268
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• 2000

할미송이버섯으로부터 혈전용해효소 (FE-2)를 DEAE-cellulose, Mono-S column으로 분리 정제하였다. 이 효소는 분자량이 18.2 kDa 이었으며, ICP-MS (inductively coupled plasma mass spectrometer) 분석 결과 $Zn^{2+}$을 포함하고 있었다. 15번째까지의 N-terminal amino acid 서열은 A-L-Y-V-G-X-S-P-X-Q-Q-S-L-L-V이고, pH 7.5에서 활성이 가장 큰 염기성 단백질 분해효소로, EDTA와 1,10-phenanthroline에 의해 활성이 감소되는 metalloprotease였다. $Mg^{2+}$, $Zn^{2+}$, Fe$^{2+}$, Co$^{2+}$의 첨가 시 활성이 증가하였으나 Hg$^{2+}$의 경우 활성이 완전히 소멸되었다. 이 효소 (FE-2)는 단백질 분해효소 저해제인 E-64 (trans-epoxysuccinyl-L-leucylamido-(4-guanidino)-butane), PMSF (phenylmethylsulfonyl fluoride), pepstatin 과 2-mercaptoethanol의 영향을 받지 않으며, 섬유소원 (fibrinogen)과 반응 시 $A\alpha$ chain과 B$\beta$ chain은 분해시키지만 $\gamma$ chain과는 반응하지 않았다.

• 한국균학회지
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• 제28권1호
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• pp.60-65
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• 2000

할미송이버섯으로부터 3단계를 거처 분자량이 각각 18 kDa(FE-1)과 18.2 kDa(FE-2)인 두 개의 혈전용해효소를 분리 정제하였다. 이 효소들은 지금까지 알려진 혈전용해 효소의 질량에 비해 비교적 작았고 두 효소(FE-1과 FE-2)의 15번째까지의 N-terminal amino acid서열은 A-L-Y-V-G-X-S-P-X-Q-Q-S-L-L-V로 같았다. FE-1은 ICP-MS의 결과 $Zn^{2+}$을 mole당 0.98 mole 포함하고 있는 metalloprotease로 pH 7.5와 $55^{\circ}C$에서 가장 큰 활성을 나타내고, 1,10-phenanthroline의 첨가 시 저하된 활성은 $Zn^{2+}$나 $Co^{2+}$의 첨가에 의해 재생되었다.

• Environmental Engineering Research
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• 제14권3호
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• pp.164-169
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• 2009

A powder form of aluminum (hydr)oxides is not suitable in wastewater treatment/filtration systems because of low hydraulic conductivity and large sludge production. In this study, aluminum (hydr)oxide-coated sand (AOCS) was used to remove phosphate from aqueous solution. The properties of AOCS were analyzed using a scanning electron microscopy (SEM) combined with an energy dispersive X-ray spectrometer (EDS) and an X-ray diffractometer (XRD). Kinetic batch, equilibrium batch, and closed-loop column experiments were performed to examine the adsorption of phosphate to AOCS. The XRD pattern indicated that the powder form of aluminum (hydr)oxides coated on AOCS was similar to a low crystalline boehmite. Kinetic batch experiments demonstrated that P adsorption to AOCS reached equilibrium after 24 h of reaction time. The kinetic sorption data were described well by the pseudo second-order kinetic sorption model, which determined the amount of P adsorbed at equilibrium ($q_e$ = 0.118 mg/g) and the pseudo second-order velocity constant (k = 0.0036 g/mg/h) at initial P concentration of 25 mg/L. The equilibrium batch data were fitted well to the Freundlich isotherm model, which quantified the distribution coefficient ($K_F$ = 0.083 L/g), and the Freundlich constant (1/n = 0.339). The closed-loop column experiments showed that the phosphate removal percent decreased from 89.1 to 41.9% with increasing initial pH from 4.82 to 9.53. The adsorption capacity determined from the closed-loop experiment was 0.239 mg/g at initial pH 7.0, which is about two times greater than that ($q_e$ = 0.118 mg/g) from the kinetic batch experiment at the same condition.

• 한국조경학회지
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• 제28권6호
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• pp.62-76
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• 2001

This study is to offer the basic information and materials for the restoration of the urban forest and the ecologically healthy and continuous green belt through the investigation and the analysis on the vegetation in Hwasan Park, Chonju-City. The flora of Hwasan Neighborhood park was composed of 86 families 230 genera, 261 species, 35 varieties, 1 formae or 298 Taxa. Among them, calculation of Pte-Q Index represented 0.60, Fisher's Index. Some characteristics of the plants in this area have represented such as Th-D1-R5 type in biological type, erect form(64.6%) in growth type, 30 taxa in naturalized plants. The life form spectra investigated from Hwasan neighborhood park was recognized as the following M"17.4%, N:11.4%, E:0.3%, Ch:0%, H:29.8%, G:6.7%, HH:0%, Th:34.2%. By the Z-M method 5 plant communities and 3 afforestation were recognized; Quercus variabilis community, Q.acutissima community, Q.serrata community, Sorbus alnifolia community, Pinus densiflora community, P.rigida afforestation, Robinia pseudo-acacia afforestation and Larix leptolepis afforestation. The actual vegetation map constructed on the grounds of the communities classified and other data. The pattern of successional trend of tree species might be suggested: L. leptoleipis, P. rigida and P. densifloralongrightarrowQ.variabilis and Q.acutissimalongrightarrowS.alnifolia and Q.serratalongrightarrowCarpinus tschonoskii community. Three measurements of species diversity (richness index(SR), Shannon-Wiener index(H'), evenness index(J')) and their relationship with community type were studied in the surveyed plant communities. Q.serrata community was higher in SR, H', J' than the other communities, whereas R.pseduo-acacia afforestation was low. Finally, The vegetation of the Hwasan neighborhood park must not allowed any more. Nothing ca be better than native states in preserving the ecosystems.tems.

• 한국학교보건학회지
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• 제6권1호
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• pp.78-92
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• 1993

The purpose of this study is to find out the stress causes of the primary students in Korea, through the three major methods, life event score, multiple regression about stress causes and G.H.Q. path-diagram. A survey was made of 889 students who were selected among students of six primary schools in seoul and Ka-pyeng, from Sep. 28 to Oct. 2. 1992. The results of this study are summarized as follows : 1. Life event score Among the children's life events, (deaths of family) (degrade of school record) (the increase of absence days) (divorce of parents) (trouble with relationship) are high ranking in life event score. Besides, (scolded by teacher and parents) (beginning of extracurricular work) are spread stress causes. In conclusion, these stress causes are shown by two aspects, home, school, and so children are under stress circumstance in both places. These stress causes vary with area and sex. 2. Multiple regression about stress causes and G.H.Q. This study analysed how a stress caused by home life affects the children's health of mind and body. The most influential factors are , and these factors vary with area and sex. 3. Path-diagram This study proved that socio-psychological school environment raises children to secede from school, and this secession that operates as a stress cause affects children's health of mind and body. This serial process is analysed by path analysis, and made out a path-diagram. In conclusion, as expected socio-psychological,school environment affects the children's health of mind and body. This is shown by two aspects : positive, negative. This stress causes vary with area and sex.

• 대한화장품학회지
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• 제37권2호
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• pp.143-152
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• 2011

본 연구에서는 참가시나무 추출물의 항산화 활성, tyrosinase 및 elastase 저해 활성, 그리고 추출물들의 성분을 확인하였다. 참가시나무는 50 % ethanol을 이용해 추출하였으며, 이 추출물을 이용해 ethyl acetate 분획, aglycone 분획을 만들어 실험에 사용하였다. 참가시나무 추출물의 DPPH (1,1-diphenyl-2- picrylhydrazyl) 소거활성($FSC_{50}$)은 aglycone 분획($8.25 \;{\mu}g$/mL)에서 가장 큰 활성을 나타내었다. Luminol 발광법을 이용한 $Fe^{3+}-EDTA/H_2O_2$ 계에서 생성된 활성산소종에 대한 aglycone 분획의 소거활성(총 항산화능, $OSC_{50}$)은 $0.039\;{\mu}g$/mL로 매우 큰 활성을 나타내었다. 참가시나무 추출물의 rose-bengal로 증감된 $^1O_2$에 의한 적혈구 파괴에 대한 세포보호 효과는 모든 분획에서 농도 의존적($1-25\;{\mu}g$/mL)으로 증가하였으며, 특히 aglycone 분획은 $10 \;{\mu}g$/mL 농도에서 ${\tau}_{50}$이 259.9 min으로 높은 세포 보호 활성을 나타내었다. 참가시나무 추출물중 aglycone 분획의 tyrosinase 저해활성($IC_{50}$)과 elastase 저해활성($IC_{50}$)은 각각 $21.82\;{\mu}g$/mL 및 $41.18\;{\mu}g$/mL 큰 활성을 보여주었다. TLC와 HPLC를 이용한 성분분석 결과 quercetin, keampferol, catechin, quercitrin, isoquercitrin, hyperoside 등이 존재함을 확인하였다. 이상의 결과들로부터 참가시나무 추출물은 활성산소종을 소거하는 항산화제로 이용가능하며, 특히 aglycone 분획의 현저한 항산화 작용 및 큰 tyrosinase 및 elastase 저해 효과로부터 이들 분획 또한 화장품원료로서 응용 가능성이 큼을 알 수 있었다.

• 한국환경생태학회지
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• 제12권2호
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• pp.119-130
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• 1998

본 연구는 인천광역시 강화도 지역의 산지형 도시녹지를 대상으로 식생구조 분석을 통한 관리방향 제시를 목적으로 하였다. 총면적 11,331ha에 대한 현존식생 분석 결과 식물군집은 19개 유형으로 분리할 수 있었는데 이중 상수리나무군집, 소나무-상수리나무군집, 신갈나무군집 등 2차림이 전체의 92.32%(10,461ha)를 차지하고 있었다. 아까시나무림 등 인공식재림은 전체의 5.40%(612ha)로서 수도권지역 다른 도시들 보다 적은 면적을 나타내었다. 총 57개 조사구에 대한 TWINSPAN에 의한 classification분석 결과 총 7개 군집으로 분리되었는데 그것은 리기다소나무(군집 A), 상수리나무(군집 B), 소나무-상수리나무(군집 C), 상수리나무-소나무 (군집 D), 소나무-서어나무-졸참나무-상수리나무(군집 E), 졸참나무-신갈나무(군집 F), 느티나무-고로쇠나무 (군집 G)이었다. 강화도 지역은 온대 중부지방에서의 일반적인 천이진행단계인 소나무림에서 상수리나무림을 거쳐 신갈나무림, 졸참나무림, 서어나무림으로 생태적 천이가 진행중인 것으로 파악되었다. 토양 pH는 평균 4.17의 강산성 토양으로서 토양환경 개선을 위한 연구가 산림식생 관리계획에서 중요한 것으로 나타났다.

• Korean Journal of Mathematics
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• 제17권3호
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• pp.283-291
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• 2009

We investigate the existence of multiple solutions (${\xi},{\eta}$) for perturbations of the parabolic system with Dirichlet boundary condition $$(0.1)\;\begin{array}{lcr}{\xi}_t=-L{\xi}+g_1(3{\xi}+{\eta})-s{\phi}_1-h_1(x,t)\;in\;{\Omega}{\times}(0,\;2{\pi}),\\{\eta}_t=-L{\eta}+g_2(3{\xi}+{\eta})-s{\phi}_1-h_2(x,t)\;in\;{\Omega}{\times}(0,\;2{\pi}).\end{array}$$ We show the existence of multiple solutions (${\xi},{\eta}$) for perturbations of the parabolic system when the nonlinearity $g^{\prime}_1,\;g^{\prime}_2$ are bounded and $3g^{\prime}_1(-{\infty})+g^{\prime}_2(-{\infty})<{\lambda}_1,\;{\lambda}_n<3g^{\prime}_1(+{\infty})+g^{\prime}_2(+{\infty})<{\lambda}_{n+1}$.

• 충청수학회지
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• 제19권2호
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• pp.141-151
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• 2006

We investigate the multiplicity of $2{\pi}$-periodic solutions of the nonlinear Hamiltonian system with almost polynomial and exponential potentials, $\dot{z}=J(G^{\prime}(z)+h(t))$, where $z:R{\rightarrow}R^{2n}$, $\dot{z}=\frac{dz}{dt}$, $J=\(\array{0&-I\\I&o}\)$, I is the identity matrix on $R^n$, $H:R^{2n}{\rightarrow}R$, and $H_z$ is the gradient of H. We look for the weak solutions $z=(p,q){\in}E$ of the nonlinear Hamiltonian system.

• Asian-Australasian Journal of Animal Sciences
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• 제16권10호
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• pp.1421-1423
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• 2003

Cross fertility between wild Japanese quail and domestic quail was explored in an experiment conducted on 18（3♂, 15♀）wild Japanese quails in Weishan Lake area, 18（3♂, 15♀）medium-sized domestic quails and 18（3♂, 15♀）pint-sized domestic quails, which were divided into nine groups. This study demonstrated that wild quail could succeed in crossing with domestic quail,producing fertilized eggs and hatching first filial generation. The findings indicated that there were no reproduction isolation between the wild Japanese quail and domestic quail, and that the best cross combination was between wild male quail and medium-sized domestic female quail, in which the fertility rate and hatchability of fertilized eggs amounted to 42.86% and 29.63% respectively. Based on the results, a new way could be adopted to protect, exploit and utilize genetic resources of wild quail.