• 제목/요약/키워드: G.H.G

검색결과 25,195건 처리시간 0.058초

Identification of Single Nucleotide Polymorphism of H-FABP Gene and Its Association with Fatness Traits in Chickens

  • Wang, Yan;Shu, Dingming;Li, Liang;Qu, Hao;Yang, Chunfen;Zhu, Qing
    • Asian-Australasian Journal of Animal Sciences
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    • 제20권12호
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    • pp.1812-1819
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    • 2007
  • Heart fatty acid-binding protein gene (H-FABP) is an important candidate gene for meat quality. One of the objectives of this study was to screen single nucleotide polymorphisms (SNP) of chicken H-FABP gene among 252 individuals that included 4 Chinese domestic chicken breeds (Fengkai Xinghua (T04), Huiyang Huxu (H), Qingyuan Ma (Q), Guangxi Xiayan (S1)), 2 breeds developed by the Institute of Animal Science, Guangdong Academy of Agricultural Sciences (Lingnan Huang (DC), dwarf chicken (E4)) and one introduced broiler (Abor Acre (AA)). Another objective of this study was to analyze the associations between polymorphisms of the H-FABP gene and fat deposition traits in chickens. PCR-SSCP was used to analyze SNPs in H-FABP and 4 SNPs (T260C, G675A, C783T and G2778A) were detected. Associations between polymorphic loci and intramuscular fat (IMF), abdominal fat weight (AFW) and abdominal fat percentage (AFP) were analyzed by ANCOVA method. The results showed that the T260C genotypes were significantly associated with IMF (p = 0.0233) and AFP (p = 0.0001); the G675A genotypes were significantly associated with AFW, AFP (p<0.01) and IMF (p<0.05); at the C783T locus, AFW and AFP differed highly between genotypes. However, the G2778A loci did not show any significant effect on fat deposition traits in this study. In addition, we found that there were some differences between AFP and definite haplotypes through a nonparametric statistical method, so the haplotypes based on the SNPs except G2778A loci were also significantly associated with IMF, AFW (g) (p<0.05) and AFP (%) (p<0.001). Significantly and suggestively dominant effects of H4H4 haplotype were observed for IMF and the H2H3 was dominant for AFW (g) and AFP (%). The results also revealed that H5H7 haplotype had a negative effect on IMF, while the H5H6 had a positive effect on AFW (g) and AFP (%).

식용유지 중 polycyclic aromatic hydrocarbons 화합물 함량 (Concentrations of Polycyclic Aromatic Hydrocarbons in Vegetable Oils and Fats)

  • 정소영;소유섭;박성국;이은주;서정혁;최우정;김정수;김미혜;권기성;이종옥;김희연;이철원
    • 한국식품과학회지
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    • 제36권4호
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    • pp.688-691
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    • 2004
  • 본 연구에서는 전국에서 유통되는 대두유, 참기름, 올리브유, 마아가린 등 식용유지 총 205건을 대상으로 대표적 PAHs 화합물인 benzo(a)pyrene 등 8개 PAHs 화합물에 대하여 분석하였다. 분석한 식용유지의 PAHs 평균 함량은 각각 benzo(a)anthracene $0.53{\mu}g/kg$, chrysene $0.82{\mu}g/kg$, benzo(b)fluoranthene $0.50{\mu}g/kg$, benzo(k)fluoranthene $0.18{\mu}g/kg$, benzo(a)pyrene $0.35{\mu}g/kg$, dibenzo(a,h)anthracene $0.16{\mu}g/kg$, benzo(g,h,i)perylene $0.31{\mu}g/kg$, indeno(1,2,3-c,d)pyrene $0.44{\mu}g/kg$이었다. 조사결과 우리나라에서 현재 유통되는 식용유지 중 benzo(a)pyrene 등 PAHs 함량은 외국의 모니터링 결과와 유사하거나 낮았다.

Reverse transcription Loop-mediated isothermal amplification을 이용한 Soybean mosaic virus의 진단 (Detection of Soybean mosaic virus by Reverse Transcription Loop-mediated Isothermal Amplification)

  • 이영훈;배대현;김봉섭;윤영남;배순도;김현주;;박인희;이수헌;강항원
    • 식물병연구
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    • 제21권4호
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    • pp.315-320
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    • 2015
  • Soybean mosaic virus(SMV)는 potyvirus 속에 속하며, 모자이크, 괴사, 기형 등의 병징을 야기하고 국내에서는 11개 계통(G1 to G7, G5H, G6H, G7H, G7a)이 보고되어있다. Reverse transcription loop-mediated isothermal amplification(RT-LAMP) 방법은 등온에서 유전자 증폭이 가능하게 하며, 이 방법은 PCR 과정이나 전기영동 없이도 바이러스에 감염된 식물을 검출할 수 있는 이점이 있다. RT-LAMP의 최적반응 조건은 $58^{\circ}C$, 60분으로 확인되었다. 특이성 검정을 위해 콩에서 발생하는 여러 바이러스들과 보유중인 SMV의 9 계통에서 그 특이성을 확인하였다. 그 결과 SMV에 대한 RT-LAMP primer들의 종 특이성이 확인되었으며, SMV의 계통들에 대해서도 적용이 가능한 것으로 확인되었다. 항온수조와 heating block과 같은 간편한 등온 장치에서 재현성을 확인하기 위해 Thermocycler 기기와 비교하여 증폭 여부를 확인한 결과 반응의 차이는 나타나지 않았다. RTLAMP 반응 이후, 반응물을 전기영동과 SYBR Green I을 이용하여 자연광과 UV광에서 증폭 여부를 확인하였다. 그 결과 전기 영동, 자연광, portable UV light와 UV transilluminator에서 모두 반응이 확인되었다.

POISSON BRACKETS DETERMINED BY JACOBIANS

  • Ahn, Jaehyun;Oh, Sei-Qwon;Park, Sujin
    • 충청수학회지
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    • 제26권2호
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    • pp.357-365
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    • 2013
  • Fix $n-2$ elements $h_1,{\cdots},h_{n-2}$ of the quotient field B of the polynomial algebra $\mathbb{C}[x_1,x_2,{\cdots},x_n]$. It is proved that B is a Poisson algebra with Poisson bracket defined by $\{f,g\}=det(Jac(f,g,h_1,{\cdots},h_{n-2})$ for any $f,g{\in}B$, where det(Jac) is the determinant of a Jacobian matrix.

각종 유기성 폐수로부터 Clostridium butyricum 및 Rhodopseudomonas sphaeroides에 의한 수소생산 (Hydrogen gas production by fermentation from various organic wastewater using Clostridium butyricum NCIB 9576 and Rhodopseudomonas sphaeroides E15-1)

  • 윤영수;김현경;유혜연;이인구;김미선
    • 한국수소및신에너지학회논문집
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    • 제11권1호
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    • pp.29-41
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    • 2000
  • Anaerobic fermentation using Clostridium butyricum NCIB 9576, and photo-fermentation using Rhodopseudomonas sphaeroides E15-1 were studied for the production of hydrogen from Makkoli, fruits (orange & apple, watermelon & melon) and Tofu wastewaters. From the Makkoli wastewater, which contained $0.94g/{\ell}$ sugars and $2.74g/{\ell}$ soluble starch, approximately $49mM\;H_2/{\ell}$ wastewater was produced during the initial 18h of the anaerobic fermentation with pH control between 6.5-7.0. Several organic acids such as butyric acid, acetic acid, propionic acid, lactic acid and ethanol were also produced. From Watemlelon and melon wastewater, which contained $43g/{\ell}$ sugars, generated about approximately $71mM\;H_2/{\ell}$ wastewater was produced during the initial 24 h of the anaerobic fermentation. Tofu wastewater, pH 6.5, containing $12.6g/{\ell}$ soluble starch and $0.74g/{\ell}$ sugars, generated about $30mM\;H_2/{\ell}$ wastewater, along with some organic acids, during the initial 24 h of anaerobic fermentation. Makkoli and Tofu wastewaters as substrates for the photo-fermentation by Rhodopseudomonas sphaeroides E15-1 produced approximately 37.9 and $22.2{\mu}M\;H_2/m{\ell}$ wastewaters, respectively for 9 days of incubation under the average of 9,000-10,000 lux illumination at the surface of reactor using tungsten halogen lamps. Orange and apple wastewater, which contained 93.4 g/l, produced approximately $13.1{\mu}M\;H_2/m{\ell}$ wastewater only for 2 days of photo-fermentation and the growth of Rhodopseudomonas sphaeroides E15-1 and hydrogen production were stopped.

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혼합폐기물 및 폴리우레탄 담체를 충전한 연속회분식공정을 이용한 생물학적 수소생산 (Biological Hydrogen Production from Mixed Waste in a Polyurethane Foam-sequencing Batch Reactor)

  • 이정열;위대현;조경숙
    • 한국미생물·생명공학회지
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    • 제42권3호
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    • pp.307-311
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    • 2014
  • 폴리우레탄 담체가 혼합폐기물을 이용한 연속식수소생산에 미치는 영향을 조사하였다. 당밀폐수와 하수슬러지를 혼합하여 연속 회분식 공정에서 발효시켰다. 담체를 넣지 않고 12 h의 수리학적 체류시간으로 운전하였을 때, 대부분의 바이오매스가 외부로 유실된 반면, 담체를 반응조에 투입하였을 때에는 미생물 유실이 현저히 저감하였다. 또한, 담체를 이용한 경우, 수소생산속도 $0.4L-H_2L^{-1}d^{-1}$로 높게 나타났다. 반응조 내 부유 바이오매스에 의한 비수소생산속도가 $241{\pm}4ml-H_2g-VSS^{-1}d^{-1}$로서 담체 표면 부착바이오매스($133{\pm}10ml-H_2g-VSS^{-1}d^{-1}$) 및 담체 내부 부착 바이오매스에 의한 값($95{\pm}14ml-H_2g-VSS^{-1}d^{-1}$)보다 높게 나타났다.