• Korean Journal of Pharmacognosy
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• v.35 no.3 s.138
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• pp.207-214
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• 2004

This study was investigated antioxidatve activity for the purpose of developing antioxidant from Morus bombycis Koidzumi. Antioxidant activities of four different organs of Morus bombycis Koidzumi such as fruit, leaf, stem, and root were examined by radical scavenging effect with 1,1-diphenyl-2-picrylhydrazyl (DPPH). 80% methanol extract from the stem showed strongly antioxidative activity and 80% Ethanol extracts from the root, stem, and fruit had high antioxidative activity among 24 samples tested. The 80% ethanol extract has strong absorbency at UVA region (350 nm). The ethyl acetate (EtOAc) fraction exhibited antioxidative activity with $IC_{50}$ of $15.0\;{\mu}g/ml$ similar to those of synthetic antioxidant, BHT The EtOAc fraction has a good absorbency property as synthetic filter. In the absorbance of various extracts, the 80% ethanol and ethyl acetate extracts from the root of Morus bombycis Koidzumi showed higher absorbancy at 285 nm. The ethyl acetate fraction from the root of Morus bombycis Koidzumi contained total phenolic compounds of 654.8 mg/100 g. These results indicate that phenolic compounds are the major was biological components in the root of morus bombycis Koidzumi extracts. Considering these biological activities, the extracts of Morus bombycis Koidzumi showed a possibility to be used as a new material for natural anti-oxidants and substitutes for synthetic UV sunscreen agents.

• Korean Journal of Plant Taxonomy
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• v.43 no.4
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• pp.319-331
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• 2013

Records found on labels of specimens deposited at Kyoto University (KYO) and references about three Japanese taxonomists, Koidzumi, Gen'ichi (1883-1953), Ohwi, Jisaburo (1905-1977), Kitamura, Siro (1906-2002) were assembled to produce collector's itineraries from 1930 to 1935 in Korea. The quality of data on labels of the specimens varies, but most are only the collector's name and country of collection, often, the locality data are only textual, and the Chinese and Japanese names, as well as the ethnic dialects common to the region, varies widely. It is estimated that approximately 2,000 specimens collected from Korea by three taxonomists are currently held within the collections of Kyoto University herbarium (KYO). Koidzumi, who was the professor of Kyoto University, traversed different northern parts of the country, such as Island Jeju-do, Mt. Keumkang-san, Hamkyongbuk-do during summer (July to August) in 1932, 1933, and 1935. In 1930 and 1932, Ohwi spend three months in the unexplored mountains in northern parts, such as Hamkyeongnam-do, Hamkyeongbuk-do, and Gangwon-do. On the other hand, for two months in the middle of 1935 visited Jeju-do, Mt. Jirisan and travelled through southern parts. Unlike two previous botanists, major collections in Korea by Kitamura took place twice in one major area in northern part and Jeju-do and Mt. Keumgang-san in 1930, 1932, and 1935.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.34 no.2
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• pp.101-107
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• 2008

In order to investigate the potential of a Angelica keiskei Koidzumi extract and its fractions as an active ingredient for whitening cosmetics, we prepared Angelica keiskei Koidzumi extract(70% ethanol), and the aqueous suspension was successively extracted with hexane, ethyl acetate(EA), and n-butanol fraction. We measured their inhibitory effects on mushroom tyrosinase and melanin synthesis in B16 melanoma cells and normal human kerationocytes in vitro. They did not show inhibitory activity against mushroom tyrosinase and the melanin synthesis except hexane and EA fractions. Hexane and EA fractions markedly inhibited cellular tyrosinase activity at a lower concentration(25 and 5 ${\mu}g/mL$, respectively) than arbutin(250 ${\mu}g/mL$). We also quantified the released amount of endothelin-1(ET-1), a mitogen of melanocyte, and interleukin-$1{\alpha}$(IL-$1{\alpha})$, a mediator of UVB-induced inflammation. Hexane and EA fractions did not affect IL-$1{\alpha}$ production, but they decreased UVB-induced ET-1 production in normal human keratinocytes in a dose dependant manner. As a result, hexane and EA fractions could inhibit the melanogenesis through the inhibition of UVB-induced ET-1 production in normal human keratinocytes. This result suggests that hexane and EA fractions of Angelica keiskei Koidzumi extract could be used as an active ingredient for cosmetics.

• Preventive Nutrition and Food Science
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• v.9 no.4
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• pp.361-366
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• 2004

Defatted methanol extracts of the medicinal plants, Rubus coreanus Miq. (RC) and Atractylodes japonica Koidzumi (AJ) were added at the levels of 0.1, 0.5, or $2\%$ (w/w) to high cholesterol diets and fed to ovariectomized Sprague-Dawley female rats, weighing 212.6 $\pm$ 1.8 g for four weeks. Weight gains were lower in RC and AJ groups than the control group, but there were no changes in uterus weights. Serum levels of triglyceride decreased by 20-$27\%$ in the experimental groups fed $0.1\%$ of each extract (O.1RC and O.1AJ), compared with that of control (Ovx). Serum cholesterol levels were not changed in the RC groups but increased in the group fed $2\%$ of the AJ extract. Liver levels of cholesterol and triglyceride were reduced in both the RC and AJ groups. Microscopic observation revealed that there were no morphological alterations in liver, lung, heart, spleen and kidney tissues of the experimental groups. Plasma levels of albumin, BUN, creatinine, sodium, potassium and phosphate in the IRC and AJ groups were in normal ranges. Serum GOT and GPT activities were, however, higher in the 2.0AJ than Ovx group. These results suggest that the extracts of the Rubus coreanus Miq. and Atractylodes japonica Koidzumi at dietary levels as low as $0.1\%$ may be utilized as hypotriglyceridemic ingredients for functional foods.

• Journal of the Korean Society of Food Science and Nutrition
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• v.45 no.10
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• pp.1391-1397
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• 2016

We investigated the hepatoprotective effects of Angelica keiskei Koidzumi extract (AK) in HepG2-overexpressing cytochrome P4502E1 (CYP2E1) and C57BL/6J mice. In HepG2 cells expressing CYP2E1, cell viability and catalase activity in the ethanol-AK co-treated group significantly increased compared to those in the ethanol-treated group. In the in vivo study with C57BL/6J mice, the AK-supplemented group with ethanol liquid diet showed significantly reduced hepatic markers, including serum aspartate aminotransferase, alanine aminotransferase, and ${\gamma}$-glutamyl transferase, compared to the ethanol group without AK supplementation. AK supplementation (20 mg/kg BW/d) also significantly attenuated reactive oxygen species generation and malondialdehyde level. Notably, a low dose of AK supplementation (20 mg/kg BW/d) suppressed expression of hepatic CYP2E1 and inhibited CYP2E1 enzyme activity. These data indicate that a low dose of AK supplementation could restrain alcohol-induced hepatic damage mediated by CYP2E1.

• Journal of Korean Society of Forest Science
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• v.99 no.4
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• pp.447-469
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• 2010

The recent publications of two major works from Ministry of Environment and Korea National Arboretum (Type specimens collected from Korea at the herbarium of the University of Tokyo, five volumes) and National Institute of Biological Resources (Korean type specimens deposited in foreign herbaria and museums, two volumes excluding other animal groups) on Korean vascular plants have resulted in discrepancies needing either correction or clarification. The former published the results of a portion of T. Nakai's collections deposited at TI, while the latter produced the results about collections of G. Koidzumi's, J. Ohwi's, and S. Kitamura's at Kyoto University and of E. H. Wilson's and H. Leveille's at Harvard University. Consequently some omissions, discrepancies, and conflicts resulted, such as the inclusion of conflicting designations of lectotype, isotype, syntype or holotype, erroneous citations of publications, and misinformation. Suh et al.'s publications about type specimens of Korean plants deposited at TI showed 47 conflicting typifications and 20 typographical and other errors. Type information offered by Yim et al. were discussed, and 75 conflicting typification and 30 typographical and other errors from these two volumes were also brought to notice. It is clear that Suh et al. and Yim et al. merely provided type information without consulting original description of each plant species. It is apparent that all these conflicting designation of types, erroneous citations of publications, and other types of misinformation were as a result of the disregard of original citation and the ignorance of basic type concept.

• The Korea Journal of Herbology
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• v.34 no.3
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• pp.1-7
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• 2019

Objectives : Amyloid ${\beta}(A{\beta})$ could induce cognitive deficits through oxidative stress, inflammation, and neuron death in Alzheimer's disease (AD). This study was investigated the effect of Angelica keiskei KOIDZUMI (AK) on memory in $A{\beta}$-induced an AD model. Methods : AK was extracted uses 70% ethanol solvent. Total polyphenol and flavonoids content were obtained by the Folin-Ciocalteu and the Ethylene glycol colorimetric methods, respectively. The antioxidant activities were assessed through free radical scavenging assays using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazolin-6-sulfonic acid) (ABTS) methods. Intracerebroventrical (i.c.v) injection of $A{\beta}$ 1-42 was used to induce AD in male ICR mice, followed by administrations of 5, 10 or 20 mg/kg AK on a daily. Animals were subjected to short and long term memory behavior in Y-maze and passive avoidance test. Results : The total polyphenol and flavonoids contents of the AK extract were $88.73{\pm}6.36mg$ gallic acid equivalent/g, $84.21{\pm}5.04mg$ rutin equivalent/g, respectively. The assays of DPPH and ABTS revealed that AK extract in treated concentrations (31.25, 62.5, 125, 250, 500, $1000{\mu}g/m{\ell}$) increased antioxidant activity in a dose-dependent manner. Oral administration of AK extract significantly reversed the $A{\beta}$ 1-42-induced decreasing of the spontaneous alternation in the Y-maze test and $A{\beta}$ 1-42-induced shorting of the step-through latency in the passive avoidance test. Conclusions : The findings suggest that AK indicated the antioxidant protective effects against $A{\beta}$-induced memory deficits, and therefore a potential lead natural therapeutic drug or agent for AD.

• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.6
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• pp.708-713
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• 2008

The effects of administration alone or mixed of Angelica keiskei and turmeric extract on blood lipids were evaluated in hypercholesterolemic diet or P-407-induced hyperlipidemic rats. In the study 1, female Sprague-Dawley rats weighing 160-180 g were divided into each groups and fed high cholesterol diets for 8 weeks. Experimental groups were administered with following diets: basal diet (Normal), high cholesterol diets (1% cholesterol). We did the oral administration for evaluation in experimental groups: C (vehicle), A (angelica extract), T (turmeric extract), AT (angelica extract, turmeric extract/ 1:1 complex). The concentrations of serum total cholesterol, and LDL-cholesterol were decreased by 6.8%, 9.8% in A group, by 22.1%, 28.8% in C group and by 28.2%, 35.6% in AT group, compared to the C group, respectively. HDL-cholesterol levels were not different among the experimental groups. In the study 2, we induced the hypertriglyceridemia in rats by intraperitoneal injection of P-407 (0.5 g/kg) once per three days. From the next day after P-407 injection beginning, we did the oral administration as the study 1. Angelica keiskei extract, turmeric extract and complex extract decreased serum triglyceride by 17.2%, 19.7% and 48.3%, respectively. These results suggested that Angelica keiskei and turmeric extract complex might have synergistic effect in lowering total cholesterol, LDL-cholesterol and triglyceride in hyperlipidemic rats.

• Journal of Korean Society of Forest Science
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• v.33 no.1
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• pp.1-32
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• 1977

This study has been made to find out more effective way of vegetation establishment on severely denuded forest land from the viewpoint of soil moisture regimes. Various environmental factors were measured to estimate soil moisture conditions of different sites. Soil moisture influence on growth of over vegetations, water requirement and drought resistance were analyzed. The efficiency of water use was also reckoned at different fertility levels and different soil moisture conditions. This research is composed of field experiment and green house experiment. Field experiment includes height growth, survival and coverage analysis of cover vegetations (Robinia pseudoacacia L., Lespedeza bicolor Turcz, Arundinella hirta Tanaka var. ciliare Koidzumi.) with 4 fertility level treatments on 3 slopes (Steep: $37^{\circ}$, Moderate: $25^{\circ}$, Gentle; $17^{\circ}$) during dry season (1 April-30 June) and wet season (1 July-10 September). At the same time temperature, relative humidity and precipitation were measured to understand the environmental changes. Soil moisture conditions were measured with soil moisture meter with 24 soil cells. Green house experiment comprised height, fresh weight and dry weight measurements of cover vegetations with 4 fertility levels and 3 moisture conditions for 70 days. The results extracted from experiments are as follews. 1. Cover vegtations have 3 patterns of water requirement at the early stage of growth. a) Robinia type has high water requirement and weaker drought resistance. b) Lespedeza type has low water requirement and stronger drought resistance. c) Arundinella type has moderate water requirement and weaker drought resistance. 2. The vegetations have different optimum fertility levels in different soil moisture supply condition. a) Robinia needs a low fertility level in dry condition and a high level in wet condition. b) Lespedeza needs only low fertility level in all conditions. c) Arundinella needs a low fertility level in dry condition and a high level in wet condition. 3. The efficiency of water use (Water/1g dry weight) by fertility levels is different from one another. Robinia and Arundinella have a good efficiency at low fertility level in dry condition and at high fertility level in wet condition. Lespedeza has a good efficiency at low fertility level in all conditions. 4. $P_2O_5$ requirement of Robinia and Lespedeza is high, but that of Arundinella is low. Soil moisture condition has a great influence on $P_2O_5$ absorption in dendued forest land. Once Vegetations are established on suitable sites with optimum fertitity level according to different moisture condition, even the small amount of soil water in denuded land can he used with high efficiency and the effect of fertility treatment can be maximized.