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Photoprotective Effect and Antioxidative Activity from Different Organs of Morus Bombycis Koidzumi  

Sa, Jae-Hoon (Gangwon Research Institute of Health and Environment)
Jin, Ying-Shan (Division of Biotechnology Kangwon National University)
Shin, In-Cheol (Gangwon Research Institute of Health and Environment)
Shim, Tae-Heum (Gangwon Research Institute of Health and Environment)
Wang, Myeong-Hyeon (Division of Biotechnology Kangwon National University)
Publication Information
Korean Journal of Pharmacognosy / v.35, no.3, 2004 , pp. 207-214 More about this Journal
Abstract
This study was investigated antioxidatve activity for the purpose of developing antioxidant from Morus bombycis Koidzumi. Antioxidant activities of four different organs of Morus bombycis Koidzumi such as fruit, leaf, stem, and root were examined by radical scavenging effect with 1,1-diphenyl-2-picrylhydrazyl (DPPH). 80% methanol extract from the stem showed strongly antioxidative activity and 80% Ethanol extracts from the root, stem, and fruit had high antioxidative activity among 24 samples tested. The 80% ethanol extract has strong absorbency at UVA region (350 nm). The ethyl acetate (EtOAc) fraction exhibited antioxidative activity with $IC_{50}$ of $15.0\;{\mu}g/ml$ similar to those of synthetic antioxidant, BHT The EtOAc fraction has a good absorbency property as synthetic filter. In the absorbance of various extracts, the 80% ethanol and ethyl acetate extracts from the root of Morus bombycis Koidzumi showed higher absorbancy at 285 nm. The ethyl acetate fraction from the root of Morus bombycis Koidzumi contained total phenolic compounds of 654.8 mg/100 g. These results indicate that phenolic compounds are the major was biological components in the root of morus bombycis Koidzumi extracts. Considering these biological activities, the extracts of Morus bombycis Koidzumi showed a possibility to be used as a new material for natural anti-oxidants and substitutes for synthetic UV sunscreen agents.
Keywords
Morus bombycis Koidzumi; antioxidant; photoprotective effect; DPPH radical scavenging activity; phenolic compound;
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