• Clinical and Experimental Pediatrics
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• 제48권8호
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• pp.857-864
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• 2005

목 적 : 인슐린 감수성 지표들은 신체계측지수인 체질량지수 및 비만도와 상관관계를 보이는 것으로 알려져 있다. 그러나 이러한 신체계측지수들이 체지방량을 잘 반영하는 것으로 알려져 있기는 하나 소아에서는 성인에 비해 그 정확도가 떨어지므로 실제로 측정한 체지방량과 인슐린 감수성 지표들 간에도 이와 같은 연관성이 있는지에 대한 연구는 없는 실정이다. 따라서 본 연구에서는 단순 소아 비만아들을 대상으로 하여, 체지방측정에 있어 비교적 객관적이며 정확한 방법으로 알려진 BIA와 DEXA로 측정한 체지방량과 인슐린 감수성 지표들 간에 어떠한 연관성이 있는지 알아보고 추후 외래에서 비만아들을 추적 관찰할 때 어느 지표가 가장 유용한지 알아보고자 한다. 방 법 : 2002년 1월부터 2004년 7월까지 중앙대학교 용산병원 비만클리닉을 방문한 28명의 단순비만 환자를 대상으로 하였다. 12시간 이상 금식 후 채혈을 하여 혈당과 인슐린 수치를 측정하였다. 같은 날 신장, 체중, 허리 둘레, 엉덩이 둘레를 계측하였고, BIA와 DEXA를 시행하여 체지방량을 구하였다. 얻어진 혈당과 인슐린 수치를 이용하여 G/I ratio, $log_{insulin}$, HOMA-IR, $log_{HOMA-IR}$, QUICKI를 구하여 체지방량과의 연관성을 알아보았다. 결 과 : 1) 인슐린 감수성 지표 중 G/I ratio만 triglyceride와 통계상 연관성이 있는 것으로 나왔다(r=-0.206, P<0.05). 이외의 $log_{insulin}$, HOMA-IR, $log_{HOMA-IR}$, QUICKI는 혈중 지질치와 관련이 없었다. 2) 비만도는 G/I ratio와 -0.209(P<0.05), $log_{insulin}$과 0.196(P<0.05), HOMA-IR과 0.238(P<0.01), $log_{HOMA-IR}$과 0.198(P<0.05), QUICKI와는 -0.224(P<0.05)로 연관성이 있는 것으로 나왔고, 체질량지수는 G/I ratio와 -0.463(P<0.01), $log_{insulin}$과 0.417(P<0.05), HOMA-IR과 0.301(P<0.01), $log_{HOMA-IR}$과 0.403(P<0.01), QUICKI와는 -0.451(P<0.01)로 나와 비만도 보다 더 인슐린 감수성 지표들과 상관성이 높은 것으로 나타났다. 3) BIA로 측정한 체지방량과 비만도 및 체질량지수 각각에 대한 상관계수는 0.612(P<0.01), 0.316(P<0.05)으로 나왔고, DEXA로 측정한 체지방량과는 각각 0.667(P<0.01), 0.512(P<0.05)로 나와 체지방량과 비만도 및 체질량지수 사이에 연관성이 있는 것으로 밝혀졌다. 4) G/I ratio는 BIA로 측정한 체지방량 및 체지방률과 각각 상관계수 -0.420(P<0.05), -0.366(P<0.05)으로 상관성을 보이고 있었고, DEXA로 측정한 체지방량 및 체지방률과도 -0.512(P<0.01), -0.449(P<0.01)로 높은 상관성을 보였다. HOMA-IR은 DEXA로 측정한 체지방량과만 상관계수 0.341(P<0.05)로 연관성을 나타냈고, 그밖에 다른 인슐린 감수성 지표들은 체지방량 및 체지방률 모두와 상관성이 없었다. 결 론 : 인슐린 감수성 지표들과 비만도 및 체질량지수와 같은 신체 계측지수들 간의 상관성에서, 인슐린 감수성 지표 특히 G/I ratio가 비만도 및 체질량지수 모두와 높은 상관성을 보였고, 체지방량 및 체지방률과 인슐린 감수성 지표와의 관계에서는 G/I ratio만이 BIA와 DEXA로 측정한 체지방량과 체지방률 모두와 연관성이 있는 것으로 나타났다. 따라서 단순 비만아의 진단 및 추적 관찰시 BMI 및 인슐린과 혈당치를 기초로 한 인슐린 감수성 지표 특히, G/I ratio의 활용이 임상에서 유용하게 사용될 수 있을 것으로 생각된다.

• 대한수의학회지
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• 제15권1호
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• pp.69-73
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• 1975

A Total of 173 streptococci isolated from 536 dairy cattle in Gyeonggi Province identified by Lancefield serological reaction and other biological properties. Antibiotics sensitivity test was also examined and the results obtained were as follows: 1. A total of 173 isolates of 167 strains (96.5%) were reacted with group A,B,C,D,E and G antisera. 2. Of the 167 positive strains, 75 strains were identified to Streptooccus uberis, 56 to Streptococcus agalactiage and 26 to Streptococcus dysgactiae, respectively. Also, Sreptococcus pyogenes, group D and group G streptococci were rarely isolated. 3. In results of antibiotic sensitivity tests, the most isolates were sensitive to leucomycin and erythromycin but less sensitive to streptomycin. and kanamycin.

• 전자공학회논문지D
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• 제35D권2호
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• pp.33-39
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• 1998

A six-beam accelerometer with self-compensated off-axis sensitivity was fabricated onthe selectively diffused (111)-oriented n/n$^{+}$/n silicon substrates by a unique porous silicon micromachining technique, which has self-stip characteristics and highly seletive formation of porous silicon layer during anodic reaction. Also, the characteristics of the fabricated accelerometer were investigated. The sensitivity of the acceleormeter added up outputs of three bridges through a summing circuit was 0.68 mV/g and the nonlinearity was less than 2% of the full scale output. The measured first resonant frequency was 4.236 kHz. When the outputs of three bridges were compared to summing output of bridges obtained through summing circuit, the normal output for Z-axis acceleration exhibited the same value s summing outputs of three bridges without reduction of sensitivity and thus the sensitivity decrease due to additional beam was compensated. Although a maximum off-axis sensitivity in one bridge of the accelerometer showed 17% of normal sensitivity, the off axis sensitivity obtained from summing output of three bridges decreased to 1.0%. Therefore, the self-elimination of off-axis sensitivity can be simply realized by obtaining the output of the sensor through summing circuit.t.

• 한국가족관계학회지
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• 제22권2호
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• pp.51-69
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• 2017

Objectives: The purpose of this study was to examine the associations and paths among parenting behaviors, cyber delinquency, and moral sensitivity in adolescents to reduce and prevent cyber delinquency. Method: The data was collected by a survey for 270 students from a middle school and a high school in G city. The valid 262 cases were analyzed by frequency, descriptive analysis with SPSS 18.0 and tested by structural equation model by using AMOS 18.0 program. Results: Results showed that 1) 'control' among the parenting behaviors has positive effects on the cyber delinquency in adolescents, 2) 'intimacy and rationality' among the parenting behaviors has positive impacts on the moral sensitivity, 3) it shows that moral sensitivity has negative effects on the cyber delinquency in adolescents, and 4) bootstrapping test for indirect effect of moral sensitivity was verified significantly. We founded the full mediation of moral sensitivity and the path of 'intimacy and rationality' to cyber delinquency through moral sensitivity. Conclusions: The results implied 1) provision of the parent education and program for parents and newlyweds to learn positive parenting behaviors and 2) application of moral education to develop moral sensitivity of adolescents in schools and communities.

• Journal of Ginseng Research
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• 제39권3호
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• pp.199-205
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• 2015

Background: Ginsenoside Rb1 (G-Rb1), the major active constituent of ginseng, improves insulin sensitivity and exerts antidiabetic effects. We tested whether the insulin-sensitizing and antidiabetic effects of G-Rb1 results from a reduction in ectopic fat accumulation, mediated by inhibition of lipolysis in adipocytes. Methods: Obese and diabetic db/db mice were treated with daily doses of 20 mg/kg G-Rb1 for 14 days. Hepatic fat accumulation was evaluated by measuring liver weight and triglyceride content. Levels of blood glucose and serum insulin were used to evaluate insulin sensitivity in db/db mice. Lipolysis in adipocytes was evaluated by measuring plasma-free fatty acids and glycerol release from 3T3-L1 adipocytes treated with G-Rb1. The expression of relevant genes was analyzed by western blotting, quantitative real-time polymerase chain reaction, and enzyme-linked immunosorbent assay kit. Results: G-Rb1 increased insulin sensitivity and alleviated hepatic fat accumulation in obese diabetic db/db mice, and these effects were accompanied by reduced liver weight and hepatic triglyceride content. Furthermore, G-Rb1 lowered the levels of free fatty acids in obese mice, which may contribute to a decline in hepatic lipid accumulation. Corresponding to these results, G-Rb1 significantly suppressed lipolysis in 3T3-L1 adipocytes and upregulated the perilipin expression in both 3T3-L1 adipocytes and mouse epididymal fat pads. Moreover, G-Rb1 increased the level of adiponectin and reduced that of tumor necrosis factor-${\alpha}$ in obese mice, and these effects were confirmed in 3T3-L1 adipocytes. Conclusion: G-Rb1 may improve insulin sensitivity in obese and diabetic db/db mice by reducing hepatic fat accumulation and suppressing adipocyte lipolysis; these effects may be mediated via the upregulation of perilipin expression in adipocytes.

• Journal of Nutrition and Health
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• 제24권5호
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• pp.450-457
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• 1991

본 연구는 생화학적 검사에 의한 철 영양상태의 판정시 검사항목에 따른 판정의 정확도를 보기 위하여 외견상 건강한 18~21세의 여대생 57명을 대상으로 혈액성분 중 Hb, Hct, serum ferritin을 측정하고 그 상관성을 분석하였다. 1) 조사대상자의 Hb는 평균이 $13.9\pm0.96g/dl,$ 중앙값이 14.1g/dl 였다. Hct의 평균은 $41.4\pm2.85%,$ 중앙값은 42.0%였고, ferritin은 평균 $20.7\pm15.5ng/ml,$ 중앙값 16.5ng/ml, 최빈값 3.40ng/ml 였다. Hb, Hct, ferritin값 모두 Kolmogorov-Smirnov test결과 정규분포 하는 것으로 나타났다. 2) Hb와 Hct간에는 r=0.9467(p<0.001)로 (Hct=2.28+2.81$\times$Hb)의 회귀관계를 보였다. Hb와 ferritin간에는 r=0.5396(p<0.001)로 $\times$Hb)를, Hct와 ferritin간에는 r=0.5591(p<0.001)로 (log(ferritin)=-1.73+0.07$\times$Hct>의 회귀식이 구해졌다. 3) 빈혈발현율은 Hb 12g/dl를 기준시 5.3%, Hct 36% 기준시 10.5%, ferritin 12ng/ml 기준시 36.8% 였다. 4) Ferritin 함량을 기준하여 Hb, Hct판정에 대한 신뢰도를 분석한 결과, 빈혈 판정시 많이 이용되는 Hb 12g/dl 미만이나 Hct 36% 이하를 기준했을 때 두 방법 모두 sensitivity가 매우 낮았으며 specificity는 매우 높은 값을 보여, 빈혈 발현율이 높은 우리나라의 경우 Hb나 Hct의 판정기준치를 높일 필요가 있는 것으로 나타났다. 5) 빈혈 판정을 위한 검사방법으로서의 Hb측정은 sensitivity, specificity 등을 고려할 때 판정 기준치를 14g/dl로 하는 것이 타당할 것으로 보인다. Hct의 경우 판정치를 40%로 높일 경우에 false-negative rate가 42.9%로 낮아졌다.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2011년도 춘계학술대회 논문집
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• pp.1561-1562
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• 2011

• Parasites, Hosts and Diseases
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• 제56권1호
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• pp.61-70
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• 2018

We developed a Rapid Diagnostic Test (RDT) kit for detecting IgG/IgM antibodies against Zika virus (ZIKV) using monoclonal antibodies to the envelope (E) and non-structural protein 1 (NS1) of ZIKV. These proteins were produced using baculovirus expression vector with Sf9 cells. Monoclonal antibodies J2G7 to NS1 and J5E1 to E protein were selected and conjugated with colloidal gold to produce the Zika IgG/IgM RDT kit (Zika RDT). Comparisons with ELISA, plaque reduction neutralization test (PRNT), and PCR were done to investigate the analytical sensitivity of Zika RDT, which resulted in 100% identical results. Sensitivity and specificity of Zika RDT in a field test was determined using positive and negative samples from Brazil and Korea. The diagnostic accuracy of Zika RDT was fairly high; sensitivity and specificity for IgG was 99.0 and 99.3%, respectively, while for IgM it was 96.7 and 98.7%, respectively. Cross reaction with dengue virus was evaluated using anti-Dengue Mixed Titer Performance Panel (PVD201), in which the Zika RDT showed cross-reactions with DENV in 16.7% and 5.6% in IgG and IgM, respectively. Cross reactions were not observed with West Nile, yellow fever, and hepatitis C virus infected sera. Zika RDT kit is very simple to use, rapid to assay, and very sensitive, and highly specific. Therefore, it would serve as a choice of method for point-of-care diagnosis and large scale surveys of ZIKV infection under clinical or field conditions worldwide in endemic areas.

• Asian Pacific Journal of Cancer Prevention
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• 제15권4호
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• pp.1667-1670
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• 2014

Background: Calprotectin in feces seems to be a more sensitive marker for gastrointestinal (GI) cancers than fecal occult blood, but its specificity may be too low for screening average risk populations. This study aims at evaluating the diagnostic value of fecal calprotectin as a screening biomarker for GI malignancies. Materials and Methods: In a case-control study, 100 patients with GI malignancies (50 patients with colorectal cancer and 50 patients with gastric cancer) and 50 controls were recruited in Tabriz Imam Reza and Sina hospitals during a 24-month period. One to two weeks after the last endoscopy/colonoscopy, fecal specimens were collected by the patients and examined by ELISA method for quantitative measurement of calprotectin content. The results were compared between the three groups. Results: The mean fecal calprotectin level was $109.1{\pm}105.3$ (2.3-454.3, median:74), $241.1{\pm}205.2$ (3.4-610.0, median:19.3) and $45.9{\pm}55.1{\mu}g/g$ (1.3-257.1, median:19.3) in gastric cancer, colorectal cancer and control group, respectively, the differences being significant (p<0.001) and remaining after adjustment for age. The optimal cut-off point for fecal calprotectin was ${\geq}75.8{\mu}g/g$ for distinguishing colorectal cancer from normal cases (sensitivity and specificity of 80% and 84%, respectively). This value was ${\geq}41.9{\mu}g/g$ for distinguishing gastric cancer from normal cases (sensitivity and specificity of 62%). Conclusions: Our results revealed that fecal calprotectin might be a useful and non-invasive biomarker for distinguishing colorectal cancer from non-malignant GI conditions. However, due to low sensitivity and specificity, this biomarker may not help physicians distinguishing gastric cancer cases from healthy subjects.

• Smart Structures and Systems
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• 제19권6호
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• pp.625-631
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• 2017

In the fields of civil engineering and seismology, it is essential to detect and tracking the vibrations, and the fiber Bragg gratings (FBGs) are typically used as sensors to measure vibrations. Where, one of the most popular and detailed approaches to use FBGs as vibration sensors involves the use of cantilever beam designs, which adds a mass to measure low and moderate frequencies (from 20 Hz up to 1 kHz) with high sensitivities (greater than 10 pm/g). The design consists of a bending strain in the cantilever that is simultaneously transferred to the FBG, resulting in a shift in the wavelength that is proportional to the strain experienced by the cantilever. In this work, we present the experimental results of a vibration sensor design using a cantilever beam to generate an axial uniform strain in the FBG in-line with the vertical axis, which modifies the cantilever's natural frequency that allows the sensor to have a wide frequency broadband without losing sensitivity. This sensor achieved a sensitivity of about 339 pm/g and a natural frequency of 227.3 Hz. The presented design compared with the traditional cantilever beam-based FBG vibration sensors, has the advantages of a simple design for detection on vibration-sensitive structures and its physical parameters can be easily modified in order to satisfy the requirements of the desired vibration measurements.