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Spray-Dried Powder Preparation and Physicochemical Properties of Green Coffee Bean Extracts (커피 생두 추출물을 이용한 분무건조 분말의 제조 및 물리화학적 품질특성)

  • Kim, Ju-Hyeon;Hong, Joo-Heon
    • Journal of Chitin and Chitosan
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    • v.23 no.4
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    • pp.234-243
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    • 2018
  • In this study, the physicochemical properties of freeze dried (FD) and spray dried powders (SD-MD10, SD-MD20, SD-CD10, SD-CD20) from green coffee bean extract were investigated. Yields were 91.46% for FD powder and 71.63~87.98% for SD powders, respectively. The moisture content of SD powders (1.39~1.86%) was lower than that of FD powder (3.19%). L, a and b value of SD powders were higher than of FD powder. The particle size of the FD powder ($147.67{\mu}m$) was higher than that of the SD powders ($54.23{\sim}66.07{\mu}m$). The water absorption index of the SD powders (0.51-0.59) were lower than that of the FD powder (0.72). The water solubility index of the SD powders, 77.26-90.07%, was higher than that of the FD powder (70.07%). The total phenolic and flavonoid contents of SD-CD10 were 20.67 g/100 g and 8.92 g/100 g, respectively. The chlorogenic acid content of SD-CD10 (12.04 g/100 g) was higher than other SD powders. The DPPH and superoxide radical scavenging activities of SD-CD10 at $1,000{\mu}g/mL$ were 88.02% and 86.89%, respectively. The SD-CD10 was better than other SD powders on protective effects against oxidative stress in L-132 cells (87.33%). In conclusion, we provided experimental evidence that spray-dried powder of green coffee bean extracts have potential as functional food materials.

Effects of photobiomodulation on different application points and different phases of complex regional pain syndrome type I in the experimental model

  • Canever, Jaquelini Betta;Barbosa, Rafael Inacio;Hendler, Ketlyn Germann;Neves, Lais Mara Siqueira das;Kuriki, Heloyse Uliam;Aguiar, Aderbal Silva Junior;Fonseca, Marisa de Cassia Registro;Marcolino, Alexandre Marcio
    • The Korean Journal of Pain
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    • v.34 no.3
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    • pp.250-261
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    • 2021
  • Background: Complex regional pain syndrome type I (CRPS-I) consists of disorders caused by spontaneous pain or induced by some stimulus. The objective was to verify the effects of photobiomodulation (PBM) using 830 nm wavelength light at the affected paw and involved spinal cord segments during the warm or acute phase. Methods: Fifty-six mice were randomized into seven groups. Group (G) 1 was the placebo group; G2 and G3 were treated with PBM on the paw in the warm and acute phase, respectively; G4 and G5 treated with PBM on involved spinal cord segments in the warm and acute phase, respectively; G6 and G7 treated with PBM on paw and involved spinal cord segments in the warm and acute phase, respectively. Edema degree, thermal and mechanical hyperalgesia, skin temperature, and functional quality of gait (Sciatic Static Index [SSI] and Sciatic Functional Index [SFI]) were evaluated. Results: Edema was lower in G3 and G7, and these were the only groups to return to baseline values at the end of treatment. For thermal hyperalgesia only G3 and G5 returned to baseline values. Regarding mechanical hyperalgesia, the groups did not show significant differences. Thermography showed increased temperature in all groups on the seventh day. In SSI and SFI assessment, G3 and G7 showed lower values when compared to G1, respectively. Conclusions: PBM irradiation in the acute phase and in the affected paw showed better results in reducing edema, thermal and mechanical hyperalgesia, and in improving gait quality, demonstrating efficacy in treatment of CRPS-I symptoms.

Growth and Seedling Quality of Hovenia dulcis Thunb. Container Seedlings (1-0) According to Fertilization Levels of Water-soluble Fertilizers (수용성 비료의 시비수준에 따른 헛개나무 용기묘(1-0)의 생장 및 묘목품질)

  • Hyeok Lee;Jun-Hyuck Yoon;Do-Hyung Lee
    • Journal of Korean Society of Forest Science
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    • v.112 no.2
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    • pp.136-144
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    • 2023
  • This study aimed to determine the optimal fertilization level for the production of Hovenia dulcis Thunb. seedlings. The height, root collar diameter, biomass, seedling quality index, and chlorophyll content of Hovenia dulcis container seedlings (1-0) according to six fertilization treatments (control, 0.5, 1.0, 1.5, 2.0, 2.5 g·L-1) were investigated. The height, root collar diameter, and biomass of Hovenia dulcis container seedlings increased with increasing fertilization, but there were no significant differences among the ≥1.5 g·L-1 treatments, and the 2.5 g·L-1 fertilization treatment was associated with significantly lower height growth. The seedling-quality index showed a high but non-significant difference in the ≥1.5 g·L-1 treatment range, and the chlorophyll content was highest in the 1.5 g·L-1 treatment group. Considering the environmental and cost aspects of fertilization, the appropriate level of fertilization required for superior container seedling production of Hovenia dulcis was 1.5 g·L-1, and a minimum fertilization treatment of ≥0.5 g·L-1 should be applied to meet the minimum seedling specifications. These results are expected to be used as basic data for establishing an efficient seedling-production system and stable supply of Hovenia dulcis, which is used as a medicinal and honey plant.

Studies on the Microbiological Distribution for Fish Products (어육 연제품의 미생물 분포에 관한 조사)

  • 문조종;안장수;곽인신;박윤수;양화영
    • Journal of environmental and Sanitary engineering
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    • v.5 no.2
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    • pp.53-62
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    • 1990
  • There has been considerable organisms the most indicative of the sanitary quality of food products. Of the suggested indices of sanitary quality of foods are coliform organism and SPC. In addition to the usual index organisms on fish products it is also necessary to determining the sanitary quality The authors have tested with 282 fish products (spring : 39, summer : 109, autumn : 112, winter . 22) 1. The range of microbial organism in fish products are as follows Spring : SPC is $13{\times}10^3\; to\; 50{\times}10^8/g$, coliform group is $16{\times}10^2\; to\; 48{\times}10^8/g$ and 2. coli is 50 to $22{\times}10^4/100g$. Summer : SPC is 70 to$64{\times}10^9/g$. coliform group is 25 to $26{\times}10^8/g$ and E. coli is 20 to $22{\times}10^4/100g$. Autumn : SPC is $10{\times}10^3\; to\; 46{\times}10^8/g$, coliform group is 200 to $20{\times}10^5/g$ and E. coli is 20 to $22{\times}10^4/100g$. Winter : SPC is $30{\times}10^3\; to\;30{\times}10^8/g$. coliform group is $21{\times}10^2\;to\;16{\times}10^3/g$ and E. coli is 20 to 790/100g. Salmonella and Staphylococcus species were not in 282 fish products.

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Effects of Beneficial Microorganisms and Mycorrhizal Fungus Colonized Rhizoplane on the Suppression of Root Rot Pathogen, Fusarium solani (근면 정착 유용 미생물과 균근균이 근부병원균, Fusarium solani의 발병억제에 미치는 영향)

  • Han, Ki-Don;Lee, Sang-Sun;Kim, Sung-Ho;Lee, Min-Woong
    • The Korean Journal of Mycology
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    • v.24 no.1 s.76
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    • pp.38-48
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    • 1996
  • The survival or colonization of beneficial organsisms and suppression of root rot of ginseng (Panax ginseng) by two distinct bacteria, Pseudomonas cepacia, Bacillus cereus and three mycorrhiza in pot soil were investigated and compared with uninoculated root. In separate inoculation, colonization of roots by P. cepacia was maintained at 6.25 (log cfu/g root) during growth for 10 days under pot culture conditions comparing to $5.62{\sim}6.19$ by mixed treatment with other organisms. Colonizations of P. cepacia were gradually decreased from 6.25 (log cfu/g root) in 10 days growth to 3.01 (log cfu/g root) in 270 days incubation period. This reduction was also investgated in combination treatments by B. cereus or F. solani. The numbers of Fusarium spp. were colonized high number in rhizosphere soil from 3.33 to 3.67 (log cfu/g root) in control within $10{\sim}60$days after treatment of pathogen F. solani, but it's numbers were markedly decreased in 270 days cultivation of plant from 3.33 to 1.02 (log cfu/g root) after treatment. In treatment of beneficial strains of P. cepacia and B. cereus, P. cepacia significantly suppressed the development of root rot from 4.3 in control to 1.2 in treatment, whereas B. cereus alone had no effect on the rate of disease suppression. The disease index $(1.8{\sim}2.3)$ in combination of two bacteria was reduced in plants inoculated with both P. cepacia and B. cereus comparing to the index (4.3) of control. As an effect of inoculation with mycorrhiza on disease suppression, suppression of root rot by F. solani was reduced to $1.2{\sim}1.6$ in disease index in treatment of Glomus albidum and Acaulospora longular comparing to 4.3 of control. In the treatment of bacterial strain P. cepacia and mycorrhizal fungus Glomus albidum, the disease suppression was apparent to 1.2 and 1.2 comparing to 4.3 of control in disease index respectively.

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A Study on Nutritional Status and Food Habits According to Obese Index of High School Girls in Seoul (서울시내 여고생들의 비만도에 따른 영양상태와 식습관에 관한 연구)

  • 안호현;김주혜;송경희
    • The Korean Journal of Food And Nutrition
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    • v.9 no.4
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    • pp.521-528
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    • 1996
  • This study was conducted to investigate the nutritional status and food habits according to obese index of high school girls in seoul. Average height, weight, obesity rate, BMI and R hrer index of subjects were 160.2cm, 52.3kg, 96.3, 20.3 and 1.27, respectively. The average food habit score of subjects was 44.17 Subject's conceived ideal weight was lower than ideal body weight. Energy, carbohydrate, fats and protein intake of subjects were 1804kcal, 70g, 41g, 272g, respectively. Subjects had lower intake of energy, calcium, iron, thiamin, reboflavin and niacin. Nutrient intake showed positive correlation with educational level of parents, monthly family income, meal times, snack times and food habit score.

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Physiological Function in vitro of Biopolymer from Bacillus coagulans CE-74 (Bacillus coagulans CE-74에 의해 생산된 Biopolymer의 생리적 기능성)

  • Lee, Seon-Ho;Choi, Hee-Jin;Son, Jun-Ho;Bae, Du-Kyung;Bae, Jong-Ho;Kim, Sung;An, Bong-Jeon;Choi, Cheong
    • Applied Biological Chemistry
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    • v.44 no.3
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    • pp.137-142
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    • 2001
  • In order to prove physiological function of biopolymer from Bacillus coagulans CE-74, in vitro experiments simulating the passive membrane transport of gastrointestinal tract were carried out using dialysis membrane. And inhibition effect of isolated biopolymer on tyrosinase and angiotensin converting enzyme (ACE) were observed. The glucose retardation index after 30 min dialysis was 43.5% in the presence of 2% biopolymer. As the dialysis period became longer, the retarding effect toward glucose absorption decreased and the effect was close to zero after 5 hr dialysis. The bile acid retardation index after 30 min dialysis was 34% and 44.2% in the presence of 1% and 2% biopolymer, respectively. The effect decreased as the dialysis time elapsed. It was measured by arosinase inhibition activity of biopolymer that inhibition effect was 48.5% in $20\;{\mu}g/{\mu}l$. In a ACE inhibition activity, biopolymer showed inhibition activity as 97% in $10\;{\mu}g/{\mu}l$.

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Investigation for freshness and nutritive components of the eggs sold in Incheon (인천지역 유통 계란의 신선도 및 영양성분 함량 조사)

  • Hong, Seong-Hee;Ra, Do-Kyung;Yun, Ga-Ri;Joung, Yun-Joung;Nam, Ji-Hyeon;Cheong, Eun-Ha;Lee, Jung-Goo;Lee, Sung-Mo
    • Korean Journal of Veterinary Service
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    • v.35 no.2
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    • pp.119-128
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    • 2012
  • This study was performed to investigate the freshness and nutritive components of eggs from Incheon area. The eggs of 64 brands collected from 7 hypermarkets and 6 brands collected from farms were examined to test the Haugh unit (HU), egg yolk index and specific gravity. HU and specific gravity were higher in farm eggs than in hypermarket eggs (P<0.05), and there was no difference in the egg yolk index. Among hypermarket eggs, HU and egg yolk index were higher in the graded eggs than in non-graded eggs (P<0.05), and specific gravity was not different between them. HU and egg yolk index of the eggs collected in March were higher than those in August (P<0.05). To monitor the bacterial contamination, total coliforms, total bacterial counts (TBC) and Salmonella enteritidis were examined. Salmonella enteritidis and total coliforms were not detected from all eggs. TBC of farm eggs were less than $10^1$ CFU/mL in all samples and TBC of hypermarket eggs were less than $10^1$ CFU/mL in 187 samples, $10^1{\sim}10^2$ CFU/mL in 3 samples and $10^3{\sim}10^4$ CFU/mL in 2 samples, respectively. Antimicrobial residues were not detected from all eggs tested. The vitamin E contents in the vitamin E enriched eggs from 6 brands hypermarket were 1.98~22.96 mg/yolk100 g ($14.04{\pm}8.81$ mg/yolk100g), and those of 5 brands among them were higher than the average of ordinary eggs. In one brand egg, vitamin E content was lower than the average of ordinary eggs. The vitamin E contents of ordinary eggs were 1.75~16.36 mg/yolk100 g (average $5.57{\pm}2.76$ mg/yolk100 g) and there were no price differences between vitamin E enriched eggs and ordinary eggs. In the contents of vitamin E, docosahexaenoic acid (DHA) and cholesterol, there were no significant differences according to the price. These results suggests that egg production, distribution, and management system should be improved for supplying fresh eggs to consumers at reasonable prices.

A Tuberculosis Contact Investigation on Health Care Workers in One Hospital (일개 병원 내 의료종사자에서의 결핵 접촉자 조사)

  • Kim, Tae Hee;Jang, Yoon Suk;Jung, Sun Ju;Kim, Yeon Jae;Pai, Hyun Joo;Oh, Sung Hee
    • Pediatric Infection and Vaccine
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    • v.23 no.2
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    • pp.94-101
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    • 2016
  • Purpose: This study aimed to describe the results of a contact investigation on health care workers after exposure to a house officer with smear-positive pulmonary tuberculosis (TB). Methods: Eighty nine out of 101 subjects who had close contact with the index patient agreed to be enrolled in the investigation. The first contact investigation was conducted approximately 30 days after the index patient's onset of symptoms, followed by the second investigation after 10 weeks. In both, clinical manifestations were studied, and chest X-ray and tuberculin skin test (TST)/QuantiFERON-TB Gold (QFT-G) in dual screening strategy were conducted. Results: The first TST resulted in positive in 34 subjects (38.2%). QFT-G was conducted on 16 subjects who tested positive in the first TST and aged under 36. Six of them (37.5%) were positive. The second TST was conducted on 41 subjects with negative results in the first TST. Seventeen (41.5%) were positive and among them, three (17.6%) showed positive QFT-G. None of the subjects were diagnosed with active TB. The probability of TB infection through contact with the index patient was 7.3% (3/41) in dual screening strategy while it was 41.5% (17/41) in TST strategy. Conclusions: This first hospital-setting contact investigation for tuberculosis in Korea revealed that latent tuberculosis infection (LTBI) rates vary depending on different diagnostic strategies. This indicates the need for systematic guidelines for diagnosing LTBI in health care workers who have professional exposure to TB.

Germination and Growth Performance of A Native Threatened Tree Species Quercus gomeziana A. Camus in Nursery Stage: Case of Bangladesh

  • Nandi, Rajasree;Dey, Soma;Hossain, Mohammed Kamal
    • Journal of Forest and Environmental Science
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    • v.36 no.1
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    • pp.1-6
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    • 2020
  • This study was conducted to investigate the effect of different pre-sowing treatments of seeds on germination and growth performance of native threatened tree species Quercus gomeziana A. Camus at the nursery of Chittagong University, Bangladesh. Furthermore, seedling growth attributes under different doses of fertilizer (urea) was also experimented to find the best dose of fertilizer on this tree species at the nursery stage for better field level growth. Seeds were placed to six pre-sowing treatments e.g. control (PT0), treated with sand paper rubbing (PT1), nicking (PT2), seeds immersed in cold water for 48 hours (PT3), seeds immersed in cold water for 7 days (PT4) and seeds sown at propagator house with increased temperature (PT5). It was found from the study that germination was started earlier (at 31 days) in treatments sand paper rubbing (PT1) and nicking (PT2). The highest germination percentage (93%) was in PT1 followed by 86% in seeds immersed in cold water for 7 days (PT4) and 80% in PT0 (control). Germination percentage was observed least (63%) in PT2 even though germination started earlier. For fertilizer dose experiment to seedlings at the nursery level, treatment FT1: 100 kg/ha (0.33679 g urea/pot/seedling) comparing with other treatments FT0: 0 kg/ha (Control), FT2: 200 kg/ha (0.67358 g urea/pot/seedling), FT3: 300 kg/ha (1.01037 g urea/pot/seedling) showed better performance in case of collar diameter (6.74 mm), number of leaves, shoot dry weight (19.74), total dry weight (28.16 g), total fresh weight (67.96 g), volume index (3904.82), sturdiness (127.69). Finally, it can be concluded that Quercus gomeziana seedlings revealed better performances under the treatment FT1 in growth and biomass production. Findings of this study will be helpful to take decision on organic fertilizer dose application to seedlings of Q. gomeziana for large scale plantation and conservation of this species.