• Journal of Food Hygiene and Safety
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• v.12 no.2
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• pp.117-124
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• 1997

A simple, rapid and simultaneous analytical method is described for the detection of Sulfonamide and Tetracycline residues, i.e., Sulfamerazine (SMR), Sulfamethazine (SMT), Sulfamonomethoxine (SMM), Sulfadimethoxine (SDM), Sulfaquinoxaline (SQN), Oxytetracycline (OXY), Tetracycline (TC), Chlortetracycline (CTC). Blank control and sulfonamide and tetracycline fortified fish muscle samples (0.5 f) were blended with octadecylsilyl (C18, 40 ${\mu}{\textrm}{m}$, 21% load, 60$\AA$) derivatized silica packing material (2 g). Blended fish samples were washed with hexane, then, benzene and dichloromethace were used for the elution of tetracycline and sulfonamide were analyzed by HPLC. Correlation coefficients of standard curves for individual sulfonamide and tetracycline isolated from fortified samples were linear (0.9993$\pm$0.0003~0.9997$\pm$0.0003, 0.9493$\pm$0.078~0.9753$\pm$0.036), respectively. The average percentage recoveries of sulfonamide and tetracycline ranged as 80.86~96.52% to 85.88~92.23%, and 30.01~37.12% to 65.89~73.40%, for the concentration range (0.1~1.0 ppm) examined, respectively. Limit of detection for sulfonamide was 0.0012 ppm for SMR in Paralichthys Olivacleus and 0.0020 ppm for SMR, 0.015 ppm for SMM in Cyprinus Carpio. The applicability of this procedure is demonstrated by separation and detection of incurred tetracycline and sulfonamide residues in fish muscle tissue.

• Korean Journal of Materials Research
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• v.19 no.10
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• pp.562-568
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• 2009

Electrospinning is a technique that produces sub-micron sized continuous fibers by electric force from polymer solutions or melts. Due to its versatile manufacturability and the cost effectiveness, this method has been recently adopted for the fabrication of one-dimensional materials. Here, we fabricated polyacrylonitrile (PAN) polymer fibers, from which uniform carbon fibers with diameters of 100-200 nm were obtained after carbonization at 800 $^{\circ}C$ in N$_2$. Special emphasis was directed to the influence of the phase separated polymer solution on the morphology and the microstructure of the resulting carbon fiber. The addition of poly(stylene-co-acrylonitile) (SAN) makes the polymer solution phase separated, which allows for the formation of internal pores by its selective elimination after electrospinning. XPS and Raman Spectroscopy were used to confirm the surface composition and the degree of carbonization. At the PAN:SAN = 50:50 in vol%, the uniform carbon fibers with diameters of 300$\sim$500 nm and surface area of 131.6 m$^2$g$^{-1}$ were obtained.

• Journal of the Korean Institute of Gas
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• v.11 no.1 s.34
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• pp.18-22
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• 2007

The fault current through the earth originated from a power line ground fault might cause arcing through the soil to an adjacent pipeline, which might bring about not only a catastrophic accident such as gas explosion and oil leakage but also a hazard to the safety of workers responsible for the maintenance and repair of the pipeline. In this paper we investigated the arcing phenomena through soil between a power line tower and a pipeline and outlined the standards for the separation distance of a buried pipeline adjacent to the power line tower.

• BMB Reports
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• v.30 no.5
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• pp.315-319
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• 1997

It was discovered that monoclonal anti-erythropoietin (EPO) antibody CFC-6 can neutralize EPO-induced cell activation. To know the binding site of CFC-6, recombinant human erythropoietin (rhEPO) was digested with Glu-C, followed by a separation using high performance liquid chromato graphy (HPLC). Each HPLC fraction was blotted on the nitrocellulose membrane and the membrane was treated with anti-EPO antibody CFC-6 and anti-mouse antibody which is modified with peroxidase. Only one spot showed the color and the fraction was sequenced by Edman degradation. The results suggest that CFC-6 recognizes amino acid sequence V63-W-Q-G-L-A-L-L-S-E72 which is a part of helix II of the EPO molecule. Binding of CFC-6 to EPO may inhibit EPO binding to its receptor, which implies that the antibody binding site and the receptor binding site are close or overlapping.

• Korean Journal of Pharmacognosy
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• v.48 no.1
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• pp.88-92
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• 2017

In the present study, we carried out quantitative analysis of chrysin and pinocembrin in Korean propolis by ultra performance liquid chromatography (UPLC) equipped with diode array detector. The separation was done using BEH C18 ($2.1{\times}50mm$, $1.7{\mu}m$) column with a mobile phase consisting of MeCN and 0.1% $H_3PO_4$ at 280 nm. The chromatographic method was validated for specificity, limit of detection, limit of quantification, linearity, precision, and accuracy. A quantitative analysis exhibited that the contents of the two compounds in Korean propolis collected from 8 inland areas except Jeju-do ranged from 3.1-46.0 mg/g. These results will be valuable as basic data for standardization of Korean propolis.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.10
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• pp.1383-1388
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• 2008

Because of high growth rate and large deposition of fat in the abdomen, the chicken has been used as a model organism for understanding lipid metabolism, fattening and growing. In this study, differentially expression of proteins in chicken liver, one of the important organs for lipid metabolism, has been investigated at four different growing stages. After separation of proteins using two-dimensional electrophoresis (2-DE), more than 700 protein spots were detected. Among them, 13 growing stage specific proteins in chicken liver were selected and further investigated by matrix-assisted laser adsorptions ionization-time of flight mass spectrometry (MALDI-TOF MS). Of these, 12 proteins were matched to existing proteins based on a database search. The identified fat-related proteins in this study were fatty acid synthase (FASN) and malic enzyme (ME1). These proteins were more highly expressed at week 32 than at other weeks. In order to confirm the differential expression, one of the proteins, FASN, was confirmed by western blotting. The identified proteins will give valuable information on biochemical roles in chicken liver, especially for lipid metabolism.

• 한국전산유체공학회:학술대회논문집
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• 1995.10a
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• pp.55-63
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• 1995

The flow field around a high-speed train including cross-wind effects has been simulated. This study solves 3-D unsteady incompressible Navier-Stokes equations in the inertial frame using the iterative time marching scheme. The governing equations are differenced with 1st-order accurate backward difference scheme for the time derivatives, 3th-order accurate QUICK scheme for the convective terms and 2nd-order accurate central difference scheme for the viscous terms. The Marker-and-Cell concept was applied to efficiently solve continuity equation, which is differenced with 2nd-order accurate central difference scheme. The 4th-order artificial damping is added to the continuity equation for numerical stability. A C-H type of elliptic grid system is generated around a high-speed train including ground. The Baldwin-Lomax turbulent model was implemented to simulate the turbulent flows. To validate the present procedure, the flow around a high speed train at constant yaw angle of $45^{\circ}\;and\;90^{\circ}$ has been simulated. The simulation shows 3-D vortex generation in the lee corner. The flow separation is also observed around the rear of the train. It has concluded that the results of present study properly agree with physical flow phenomena.

• Computers and Concrete
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• v.1 no.3
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• pp.261-284
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• 2004

Two enhancements to a recently developed plastic-damage-contact model for concrete are presented. The model itself, which uses planes of degradation that can undergo damage and separation but that can regain contact according to a contact law, is described. The first enhancement is a new damage evolution function which provides a completely smooth transition from the undamaged to the damaged state and from pre-peak to post-peak regions. The second is an improved contact function that governs the potential degree of contact with increasing opening on a crack plane. The use of a damage evolution function with a pre-peak has implications for the consistent tangent matrix/stress recovery algorithm developed for the model implementation, and amendments to this algorithm to accommodate the new function are described. A series of unpublished experimental tests on notched specimens undertaken in Cardiff in the mid 1990s are then described. These include notched beam tests as well as prismatic and cylindrical torsion tests. The tests are then considered in three dimensional finite element analyses using the modified Craft model implemented in the finite element program LUSAS. Comparisons between experimental and numerical data show reasonable agreement except that the numerical simulations do not fully describe the latter stages of the softening responses for the torsion examples. Finally, it is concluded that the torsion tests described provide useful benchmark examples for the validation of three-dimensional numerical models for concrete.

• Proceedings of the KIEE Conference
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• 2008.07a
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• pp.466-467
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• 2008

In case of a line-to-ground fault at transmission lines, a portion of fault current will flow into the earth through the footings of the faulted tower causing electrical potential rise nearby the faulted tower footings. In this situation, any buried pipelines or structures nearby the faulted tower can be exposed to the electrical stress by earth potential rise. Although many research works has been conducted on this phenomena, there has been no clear answer of the required separation distance between tower footings and neary buried pipeline because of its dependancy on the soil electrical charactersics of the concerned area and the faulted system.

• BMB Reports
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• v.34 no.5
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• pp.415-420
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• 2001

Chloramphenicol acetyltransferase (CAT) was purified to homogeneity from Morganella morganii starting with ammonium sulphate fractionation, followed by separation on DEAE-Sephadex A50, and G-100 Sephadex gel filtration. The enzyme was purified 133.3 fold and showed a final specific activity of 60 units/mg protein with a yield of 37%. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of the purified enzyme revealed it as a heterotetramer that consists of four subunits with close molecular weights (19.5, 19, 18, and 17.5 kDa). The molecular weight of the native enzyme was calculated to be 78 kDa, as determined by gel filtration, which approximated to that of the four subunits (74 kDa). The enzyme showed a maximum activity at pH 7.8 when incubated at $35^{\circ}C$. A Lineweaver-Burk analysis gave a Km of 5.0 uM and Vmax of 153.8 U/ml. The amino acid composition of the purified enzyme was also determined.