• Archives of Pharmacal Research
• /
• v.28 no.10
• /
• pp.1142-1146
• /
• 2005

Seven sesquiterpene lactones were isolated by the chromatographic separation of the MeOH extract of the aerial parts of Saussurea calcicola (Compositae). Their structures were determined spectroscopically to be cynaropicrin (1), arguerin B (2), cebellin F (3), 8${\alpha}$-hydroxy-11${\alpha}$, 13-dihydrozaluzanin C (4), desacylcynaropicrin (5), 3${\beta}$-hydroxy-8${\alpha}$-epoxymethylacriloiloxy-4(15), 10(14), 11(13)-trien-guaian-6, 12-olide (6), and kandavanolide (7). Compounds 1 and 2 showed significant cytotoxicity against five cultured human tumor cell lines with $ED_{50}$ values ranging from $0.23{\~}1.72\;{\mu}g/mL$.

• Proceedings of the KIEE Conference
• /
• 2007.07a
• /
• pp.384-385
• /
• 2007

The fault current through the earth originated from a power line ground fault might cause arcing through the soil to an adjacent pipeline, which might bring about not only a catastrophic accident such as gas explosion and oil leakage but also a hazard to the safety of workers responsible for the maintenance and repair of the pipeline. In this paper we experimented on the soil breakdown test using the fixture and outlined the standards for the separation distance of a buried pipeline adjacent to the power line tower.

• KSBB Journal
• /
• v.8 no.3
• /
• pp.287-294
• /
• 1993

For the purpose of combining the purification processes for several biologically active proteins form human urine, an efficient integrated fractionation procedure has been investigated. The procedure was started by concentration with ultrafiltration and pH precipitation followed by a selectable combination of chromatography on gel filtration, adsorption, ion exchanger, affinity, and reverse phase column. By this process, the purified urokinase, epidermal growth factor and albumin migrated as a single band on SDS-polyacrylamide gel electrophoresis and were fully active. The recoveries of these purified proteins were 48%, 17%, and 46%, respectively.

• Proceedings of the Korean Nuclear Society Conference
• /
• 2005.10a
• /
• pp.241-242
• /
• 2005

• Structural Engineering and Mechanics
• /
• v.41 no.6
• /
• pp.735-750
• /
• 2012

This paper presents a comparison between two different procedures to deal with the geometric nonlinear analysis of space trusses, considering its structural stability aspects. The first nonlinear formulation, called positional, uses nodal positions rather than nodal displacements to describe the finite elements kinematics. The strains are computed directly from the proposed position concept, using a Cartesian coordinate system fixed in space. The second formulation, called corotational, is based on the explicit separation between rigid body motion and deformed motion. The numerical examples demonstrate the performances and the convergence of the responses for both analyzed formulations. Two numerical examples were compared, including a lattice beam with postcritical behavior. Despite the two completely different approaches to deal with the geometrical nonlinear problem, the results present good agreement.

• Journal of the Korean Magnetics Society
• /
• v.5 no.5
• /
• pp.524-527
• /
• 1995

The temperature dependence of spin wave mode separation in amorphous $Co_{89.5}Zr_{10.5}$ thin film has been investigated at temperatures between 100 K and 300 K. The magnetization and the spectroscopic splitting factor were obtained for the main resonance mode in parallel and perpendicular magnetic field. ${\Delta}H_{2-3}$, the difference between resonance field of mode 2 and the resonance field of mode 3, increases with decreasing temperature. The linewidth increases for all the modes with decreasing temperature. Especially in mode 3 it increases rapidly below 200 K. This phenomenon could be caused by the increase of exchange stiffness constant or the decrease of surface magnetic anisotropy constant with decreasing temperature.

• Analytical Science and Technology
• /
• v.32 no.6
• /
• pp.217-224
• /
• 2019

In this study, to evaluate the effectiveness and safety of oral therapy using Gastrodiae Rhizoma, a new HPLC-PDA analysis method was developed for the simultaneous quantitation of the three major components: (1) gastrodin, (2) gastrodigenin, and (3) p-hydroxybenzaldehyde, in steam processed and unprocessed tubers of Gastrodia elata Blume. The clear separation of the three components was achieved on a C18 column (250 × 4.6 mm, 5 ㎛) by gradient elution using water (including 0.1 % formic acid) and acetonitrile as the mobile phase. The flow rate was 1.0 mL/min, and the UV detector wavelength was set at 270 nm. The results demonstrate satisfactory linearity, recovery, precision, accuracy, stability, and robustness. The established HPLC-PDA method was applied to quantify three major compounds in 59 samples of G. elata Blume tubers. Finally, the steam processed and unprocessed tubers of G. elata Blume were successfully distinguished by pattern recognition analysis.

• Korean Journal of Veterinary Research
• /
• v.43 no.3
• /
• pp.429-433
• /
• 2003

This study was aimed to develop a more rapid, simple and sensitive method to determine ceftiofur in bovine serum using LC/MS with electrospray interface. Separation was achieved on the Nova-Pak $C_{18}$ reverse phase column. The mobile phase consisted of 0.1% acetic add in water (A) and acetonitrile (B) and gradiently flowed at the rate of 0.4 mL/min. As a result of analysis of blank muscle samples, matrix interference was not shown. Limit of detection and limit of quantitaion was 5 ng/g and 20 ng/g, respectively. The values of precision and recovery satisfied the guideline of NVRQS. The precision and recovery developed in this method are suitable and sensitive to determine the concentration of ceftiofur in the bovine serum. These results could be applied for the confirmation and quantification in the biofluid.

• Food Science and Biotechnology
• /
• v.18 no.4
• /
• pp.978-984
• /
• 2009

An analytical method for the simultaneous determination of 9 quinolones (QNs) in porcine, chicken, and bovine muscles was developed and validated using liquid chromatography-fluorescence detector (LC-FLD). The samples were extracted using a liquid-liquid extraction (LLE) process. Chromatographic separation was achieved on a reverse phase $C_8$ column with a gradient elution using a mobile phase of 200 mM ammonium acetate buffer (pH 4.5) and acetonitrile (ACN). The proposed method was validated according to the Food and Drug Administration (FDA) guideline for bioanalytical assay procedures. Recoveries of QNs were 83.1-111.9% with relative standard deviations (RSDs) below 15%. Linearity within a range of 30-500 ${\mu}g/kg$ was obtained with the correlation coefficient ($R^2$) of 0.9967-0.9999. The limits of detection (LOD) were 1-16 ${\mu}g/kg$. These values were lower than the maximum residues limits (MRLs) established by the European Union (EU). The present method was successfully applied to determine QNs in edible muscles.

• Journal of Pharmaceutical Investigation
• /
• v.29 no.2
• /
• pp.99-103
• /
• 1999

Self-Emulsifying System(SES), an isotropic mixture of oil and surfactant which forms oil-in-water emulsion, is expected to improve in vitro drug dissolution and enhance in vivo drug absorption. A poorly water soluble drug, ibu-profen(IBP) was incorporated into the SES to improve absorption, and enhance bioavailability of drug. Medium chain triglyceride, glyceryl tricaprylate(GTC) as an oil, and Tween 85 as a surfactant were used to formulate SES. To characterize SESs with various concentrations of Tween 85, the phase separation and solubility of IBP-SEDDS containing IBP as a function of Tween 85 concentration were conducted, and the particle size was measured using photon correlation spectroscopic method. The SES with optimal concentration of Tween 85(35%(w/w)) was selected based on its high drug loading, small particle size and low surfactant concentration. After an oral administration of IBP-SEDDS and IBP suspension in methyl cellulose equivalent to 40.0 mg/kg to rats, the pharmacokinetic parameters were compared. The $C_{max}(163.17\;vs\;88.82\;{\mu}g/ml)$, $AUC(12897.01\;vs\;8751.13\;{\mu}g\;min/ml)$ and Bioavailability(86.44 vs 58.65%) significantly increased but $T_max(10\;vs\;20\;min)$ was significantly advanced. The current SEDDS containing IBP provide an alternative to improve an oral bio-availability of IBP.